Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,412,786 G→T 100% E42* (GAA→TAA)  ulaE → L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,412,7860GT98.6% 929.9 / 2.9 290E42* (GAA→TAA) ulaEL‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (161/124);  total (163/127)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 290 total aligned reads displayed.

GAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCG  >  CP009273/4412639‑4412932
                                                                                                                                                   |                                                                                                                                                  
gAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTaa                                                                                                                                                  >  2:2221938/1‑150 (MQ=255)
  aCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAAc                                                                                                                                                <  2:5165756/150‑1 (MQ=255)
      tgGGGCTAAGGAGCGGATATGTTGTCCAAACAACTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGAc                                                                                                                                            <  2:3591783/150‑1 (MQ=255)
      tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGACATGTCGGTAGATTAAACTGAc                                                                                                                                            >  1:4122640/1‑150 (MQ=255)
      tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGAc                                                                                                                                            >  1:1424321/1‑150 (MQ=255)
      tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGAc                                                                                                                                            >  1:5153021/1‑150 (MQ=255)
       ggggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACg                                                                                                                                           >  2:3885648/1‑150 (MQ=255)
       ggggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACg                                                                                                                                           <  2:2151558/150‑1 (MQ=255)
        gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGa                                                                                                                                          >  2:4417305/1‑150 (MQ=255)
        gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGa                                                                                                                                          <  1:1063156/150‑1 (MQ=255)
         ggCGAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGAt                                                                                                                                         >  2:1080779/1‑150 (MQ=255)
           cTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCg                                                                                                                                       >  2:584943/1‑150 (MQ=255)
           cTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCg                                                                                                                                       >  1:628879/1‑150 (MQ=255)
           cTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCg                                                                                                                                       <  1:51763/150‑1 (MQ=255)
             aaGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGcc                                                                                                                                     >  1:443050/1‑150 (MQ=255)
              aGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                                    <  2:4903115/150‑1 (MQ=255)
               ggAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTg                                                                                                                                   <  1:857822/150‑1 (MQ=255)
                gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCACAAACGTGAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGt                                                                                                                                  >  2:1007870/1‑150 (MQ=255)
                 atcGGATATGTTCTCCAAACAAAACACGCTAGGCATCTAAGAAAAAGACCTCCCCGCCGCGCAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTAAGCCTTCGAATTACTCGAAATGTCGGTATAGAAAACAGACGAACGcaagtc                                                                                                                                 >  2:2291163/3‑145 (MQ=255)
                      atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGcc                                                                                                                            <  2:1850683/150‑1 (MQ=255)
                      atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGcc                                                                                                                            >  2:1019388/1‑150 (MQ=255)
                       tatGTTGTCCACACAAATCCCGCTTGGCATCTATGACAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCt                                                                                                                           <  2:5067105/150‑1 (MQ=255)
                            tgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACt                                                                                                                      >  1:2979119/1‑150 (MQ=255)
                            tgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACt                                                                                                                      <  1:3257405/150‑1 (MQ=255)
                               ccAAACAACTCCCGCTTGGCATCTATGAAAAAGCGCGCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTGAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGa                                                                                                                   <  1:5255770/150‑1 (MQ=255)
                               ccAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGa                                                                                                                   <  1:1332541/150‑1 (MQ=255)
                                caaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAg                                                                                                                  <  1:3056834/150‑1 (MQ=255)
                                 aaacaaaTCCCGCTTGGCATCTAGGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGTCAAAAACGTTAGGCTTCGATTTTGTCTAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGc                                                                                                                 >  1:373626/1‑150 (MQ=255)
                                  aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGcc                                                                                                                >  1:5069792/1‑150 (MQ=255)
                                       aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                           >  1:4664587/1‑150 (MQ=255)
                                       aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                           >  2:5034305/1‑150 (MQ=255)
                                       aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                           >  2:1953791/1‑150 (MQ=255)
                                         cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagca                                                                                                         >  1:1205791/1‑150 (MQ=255)
                                            gCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCg                                                                                                      >  2:1166811/1‑150 (MQ=255)
                                               tGGCATCTATGACACAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGGTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt                                                                                                   <  2:4251004/150‑1 (MQ=255)
                                               tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt                                                                                                   >  1:3312223/1‑150 (MQ=255)
                                                ggCATCTATTAAAAAGCGCTCCCCGCCGGCGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTATGCTTCGATTTTGACGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCTTcag                                                                                                  >  2:1200545/1‑148 (MQ=255)
                                                ggCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTg                                                                                                  >  2:5096069/1‑150 (MQ=255)
                                                  cATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATc                                                                                                                                        <  1:2739107/110‑1 (MQ=255)
                                                  cATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgc                                                                                                >  1:2779287/1‑150 (MQ=255)
                                                  cATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgc                                                                                                <  1:4373322/150‑1 (MQ=255)
                                                    tCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcgc                                                                                              >  1:747394/1‑150 (MQ=255)
                                                     cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCt                                                                                             >  1:2222500/1‑150 (MQ=255)
                                                      tATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAGTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                                                                                            >  2:2313377/1‑150 (MQ=255)
                                                        tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGt                                                                                          <  2:242791/150‑1 (MQ=255)
                                                           aaaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCaa                                                                                       >  1:3525622/1‑150 (MQ=255)
                                                            aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAAt                                                                                      <  2:3213909/150‑1 (MQ=255)
                                                            aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAAt                                                                                      >  2:3666739/1‑150 (MQ=255)
                                                            aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAAt                                                                                      >  2:3666803/1‑150 (MQ=255)
                                                              aGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGc                                                                                    <  1:2041384/150‑1 (MQ=255)
                                                                 gcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACCTTAGGCTTCGATTTTGTCGAAATGTCGGTAGAATAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGac                                                                                 <  1:4089169/150‑2 (MQ=255)
                                                                         ccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaaa                                                                         <  1:4203774/150‑1 (MQ=255)
                                                                         ccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaaa                                                                         <  1:4258525/150‑1 (MQ=255)
                                                                           ggggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAAcc                                                                       <  2:2973472/150‑1 (MQ=255)
                                                                           ggggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAAcc                                                                       >  1:1839342/1‑150 (MQ=255)
                                                                            gggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCg                                                                      >  1:2149021/1‑150 (MQ=255)
                                                                            gggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCg                                                                      >  2:3933203/1‑150 (MQ=255)
                                                                             ggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCgg                                                                     >  1:1599105/1‑150 (MQ=255)
                                                                               aGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcg                                                                   <  2:1562201/150‑1 (MQ=255)
                                                                               aGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcg                                                                   <  1:2456805/150‑1 (MQ=255)
                                                                                     ggctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcg                                                             <  1:3231377/150‑1 (MQ=255)
                                                                                      gattgAACGCCTGCAACTGGCAAAAACGTTAGTCTTCGATTTTGTCTAAATGTCTGTAGATTAAACTGACGATCTCCTGTAGAGCCTCAACTGGATACGCGAGCAGCGTCTGGAGCTGGTCAATGATATTGTTGAAACCTgcgtgcgcgt                                                            >  2:3055891/5‑150 (MQ=255)
                                                                                       ctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgtg                                                           <  2:1384575/150‑1 (MQ=255)
                                                                                              gCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCa                                                    <  2:1710604/150‑1 (MQ=255)
                                                                                               ccTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAt                                                   <  1:4797198/150‑1 (MQ=255)
                                                                                                cTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtg                                                  >  2:4104494/1‑150 (MQ=255)
                                                                                                cTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtg                                                  >  2:6702/1‑150 (MQ=255)
                                                                                                  gCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtgtg                                                <  1:1776499/150‑1 (MQ=255)
                                                                                                  gCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATGGTTGAAACCGGCGTGCGCGTGCCGTCCAtgtg                                                >  2:893031/1‑150 (MQ=255)
                                                                                                   cAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGc                                               >  1:307313/1‑150 (MQ=255)
                                                                                                     aCTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCt                                             <  2:756894/150‑1 (MQ=255)
                                                                                                        ggCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTc                                          <  1:26730/150‑1 (MQ=255)
                                                                                                           aaaaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGc                                       >  2:1604181/1‑150 (MQ=255)
                                                                                                           aaaaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGc                                       >  1:4306285/1‑150 (MQ=255)
                                                                                                            aaaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCt                                      <  2:3516387/150‑1 (MQ=255)
                                                                                                             aaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTc                                     >  2:3797244/1‑150 (MQ=255)
                                                                                                              aaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCa                                    >  2:1443592/1‑150 (MQ=255)
                                                                                                                cGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtc                                  >  1:1954196/1‑150 (MQ=255)
                                                                                                                cGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtc                                  >  1:1032539/1‑150 (MQ=255)
                                                                                                                 gTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcg                                 >  1:2296298/1‑150 (MQ=255)
                                                                                                                   tAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                               >  2:2279555/1‑150 (MQ=255)
                                                                                                                     ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATcgtcgt                             >  1:3929370/1‑150 (MQ=255)
                                                                                                                      gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGtt                            >  1:2980617/1‑150 (MQ=255)
                                                                                                                      gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGtt                            <  2:4645212/150‑1 (MQ=255)
                                                                                                                          cGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTccc                        <  2:1515162/150‑1 (MQ=255)
                                                                                                                          cGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTccc                        >  2:2697133/1‑150 (MQ=255)
                                                                                                                           gATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCg                       >  2:3315547/1‑150 (MQ=255)
                                                                                                                           gATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTACCg                       >  1:236372/1‑150 (MQ=255)
                                                                                                                             ttttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCt                     <  2:1503509/150‑1 (MQ=255)
                                                                                                                               ttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTgg                   >  1:4033166/1‑150 (MQ=255)
                                                                                                                                 gTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGc                 >  2:130027/1‑150 (MQ=255)
                                                                                                                                          tCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGAt        <  1:5034305/150‑1 (MQ=255)
                                                                                                                                          tCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGAt        <  2:2855728/150‑1 (MQ=255)
                                                                                                                                            ggTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGa      >  2:25344/1‑150 (MQ=255)
                                                                                                                                             gTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAc     <  1:982365/150‑1 (MQ=255)
                                                                                                                                              tAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCCGTGAAGATGAcg    >  1:1485567/1‑150 (MQ=255)
                                                                                                                                              tAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAcg    <  1:4581849/150‑1 (MQ=255)
                                                                                                                                               aGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAcgc   >  1:270336/1‑150 (MQ=255)
                                                                                                                                                gATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAcgcg  >  2:1948232/1‑150 (MQ=255)
                                                                                                                                                gATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAcgcg  <  2:1121852/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCG  >  CP009273/4412639‑4412932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑