Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,456,233 G→T 100% I277I (ATC→ATA)  treR ← trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,456,2330GT99.3% 964.4 / ‑2.2 303I277I (ATC→ATA) treRtrehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (140/161);  total (140/163)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 303 total aligned reads displayed.

GAAGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAAT  >  CP009273/4456086‑4456379
                                                                                                                                                   |                                                                                                                                                  
gAAGATCACAATTTATCGTTCAGGAATCGATCAGGACAGGGTGGCGGGGAGGATGATTTGTTGCGGGTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAt                                                                                                                                                  <  2:1422279/150‑1 (MQ=255)
gAAGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAt                                                                                                                                                  <  2:2275563/150‑1 (MQ=255)
  aGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAtct                                                                                                                                                >  1:4567291/1‑150 (MQ=255)
   gATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAtctc                                                                                                                                               >  1:1380071/1‑150 (MQ=255)
    aTCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTcc                                                                                                                                              <  1:2015744/150‑1 (MQ=255)
       acaATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGa                                                                                                                                           <  2:4583123/150‑1 (MQ=255)
        caATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGAt                                                                                                                                          <  1:4412713/150‑1 (MQ=255)
         aaTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATg                                                                                                                                         <  2:154654/150‑1 (MQ=255)
         aaTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATg                                                                                                                                         <  2:473277/150‑1 (MQ=255)
         aaTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATg                                                                                                                                         <  2:3096941/150‑1 (MQ=255)
          aTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATgg                                                                                                                                        >  1:2634246/1‑150 (MQ=255)
           gtgaTCGTTCAGGAATCGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATgga                                                                                                                                       <  2:5063645/147‑1 (MQ=255)
            ttATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggag                                                                                                                                      <  2:62083/150‑1 (MQ=255)
            ttATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggag                                                                                                                                      <  1:5061686/150‑1 (MQ=255)
             tATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggagg                                                                                                                                     >  2:2176273/1‑150 (MQ=255)
              aTCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGCTggagga                                                                                                                                    >  2:4622390/1‑150 (MQ=255)
               tCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGaa                                                                                                                                   >  2:1260118/1‑150 (MQ=255)
                cGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAt                                                                                                                                  >  2:1524065/1‑150 (MQ=255)
                 gTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGCTTGGTTGCGGTTCGCTGCGCCCGGTGACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAtt                                                                                                                                 <  2:1604014/150‑1 (MQ=255)
                  ttCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAttt                                                                                                                                >  2:4238182/1‑150 (MQ=255)
                  gtCAGGAACCGATCAGGCCCGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAttt                                                                                                                                <  1:1494100/149‑1 (MQ=255)
                     aGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCAt                                                                                                                             <  2:3340966/150‑1 (MQ=255)
                       gAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTa                                                                                                                           <  1:4933476/150‑1 (MQ=255)
                         aaCGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAAc                                                                                                                         <  1:3386861/150‑1 (MQ=255)
                            gATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGc                                                                                                                      >  2:563548/1‑150 (MQ=255)
                            gATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGc                                                                                                                      >  2:4167799/1‑150 (MQ=255)
                            gATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGc                                                                                                                      >  2:1742807/1‑150 (MQ=255)
                            gATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGc                                                                                                                      >  2:4035273/1‑150 (MQ=255)
                              tCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGt                                                                                                                    >  1:3010492/1‑150 (MQ=255)
                              tCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGGTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGt                                                                                                                    <  2:341319/150‑1 (MQ=255)
                                aGGACCGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTAt                                                                                                                  <  2:4810663/150‑1 (MQ=255)
                                  gACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTa                                                                                                                >  1:4258865/1‑150 (MQ=255)
                                    cAGGGGGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTAcc                                                                                                              <  1:4965264/150‑1 (MQ=255)
                                     aGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCg                                                                                                             <  2:1154028/150‑1 (MQ=255)
                                     aGGGGGGCGGGGATGATGATTTGGGGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCg                                                                                                             <  2:2962925/150‑1 (MQ=255)
                                         tGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGc                                                                                                         >  2:2692458/1‑150 (MQ=255)
                                         tGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGc                                                                                                         >  1:4784288/1‑150 (MQ=255)
                                         tGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGc                                                                                                         >  2:1208450/1‑150 (MQ=255)
                                          ggCGGGGTTGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCt                                                                                                        <  1:9625/150‑1 (MQ=255)
                                          ggCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCt                                                                                                        <  2:1117746/150‑1 (MQ=255)
                                          ggCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCt                                                                                                        <  2:5278687/150‑1 (MQ=255)
                                             ggggATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGc                                                                                                     >  1:900554/1‑150 (MQ=255)
                                              gggATGATGATTAGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGcc                                                                                                    >  2:2865738/1‑150 (MQ=255)
                                                 atgatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGt                                                                                                 >  1:246721/1‑150 (MQ=255)
                                                   gatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTg                                                                                               >  2:5196297/1‑150 (MQ=255)
                                                   gatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTg                                                                                               <  1:288390/150‑1 (MQ=255)
                                                     tgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCa                                                                                             >  2:3431173/1‑150 (MQ=255)
                                                       atTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAg                                                                                           <  1:133911/150‑1 (MQ=255)
                                                         ttgttgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGgt                                                                                         <  2:3924764/150‑1 (MQ=255)
                                                               cGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGAt                                                                                   <  1:3469023/150‑1 (MQ=255)
                                                                ggTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATg                                                                                  >  2:2627501/1‑150 (MQ=255)
                                                                  ttCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCg                                                                                >  1:727667/1‑150 (MQ=255)
                                                                  ttCGCTGCGCCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCg                                                                                <  2:2093939/150‑1 (MQ=255)
                                                                          gcCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCtt                                                                        <  1:4238182/150‑1 (MQ=255)
                                                                              ggTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAg                                                                    >  1:2283247/1‑150 (MQ=255)
                                                                              ggTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAg                                                                    >  1:1002352/1‑150 (MQ=255)
                                                                               gTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAgg                                                                   <  1:3539995/150‑1 (MQ=255)
                                                                                 tACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTTTTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTa                                                                 <  2:2797464/150‑1 (MQ=255)
                                                                                   ccTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAtt                                                               >  2:2396139/1‑150 (MQ=255)
                                                                                    cTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCGTGCAGGTAttt                                                              <  2:635112/150‑1 (MQ=255)
                                                                                     tGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTCTCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTa                                                             >  2:1912783/1‑150 (MQ=255)
                                                                                     tGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTa                                                             >  1:2720426/1‑150 (MQ=255)
                                                                                     tGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTa                                                             >  2:2649062/1‑150 (MQ=255)
                                                                                     tGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTa                                                             >  2:3188674/1‑150 (MQ=255)
                                                                                       cgcgATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGGTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACt                                                           <  2:5287775/150‑1 (MQ=255)
                                                                                         cgATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTg                                                         <  1:4668988/150‑1 (MQ=255)
                                                                                          gATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgc                                                        <  1:3202916/150‑1 (MQ=255)
                                                                                                cTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAg                                                  <  2:2132241/150‑1 (MQ=255)
                                                                                                 tGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGt                                                 >  1:1622831/1‑150 (MQ=255)
                                                                                                 tGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGATGCTCTTGCAGGTATTTACTTGCGCCAAGt                                                 >  1:4648341/1‑150 (MQ=255)
                                                                                                  ggCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTACTGATTGAGGAATTTCATTAACGGCGTATTACCGACGCTCGACAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTg                                                <  1:3923603/150‑1 (MQ=255)
                                                                                                     aaGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCa                                             <  1:4093983/150‑1 (MQ=255)
                                                                                                            cTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTc                                      <  2:2461285/150‑1 (MQ=255)
                                                                                                             tGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCg                                     >  2:4843888/1‑150 (MQ=255)
                                                                                                               gCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGt                                   <  2:1870108/150‑1 (MQ=255)
                                                                                                                cGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGtt                                  <  1:4192608/150‑1 (MQ=255)
                                                                                                                 gTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTg                                 >  1:5288346/1‑150 (MQ=255)
                                                                                                                   ccAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTgcg                               <  1:2367233/150‑1 (MQ=255)
                                                                                                                     aGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGca                             >  1:4122915/1‑150 (MQ=255)
                                                                                                                      gCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGcac                            <  2:3661645/150‑1 (MQ=255)
                                                                                                                       cTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGcaca                           >  1:4542059/1‑150 (MQ=255)
                                                                                                                         tCGGCGTAACCGGGATCGACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGt                         <  1:2584319/150‑1 (MQ=255)
                                                                                                                           ggCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTaa                       <  2:4438980/150‑1 (MQ=255)
                                                                                                                             cGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAgg                     <  2:360286/150‑1 (MQ=255)
                                                                                                                              gTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGc                    >  1:1846316/1‑150 (MQ=255)
                                                                                                                              gTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGc                    >  2:587562/1‑150 (MQ=255)
                                                                                                                                aaCCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCgg                  >  1:1541408/1‑150 (MQ=255)
                                                                                                                                  ccGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTa                <  2:1990396/150‑1 (MQ=255)
                                                                                                                                  ccGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTa                <  2:894638/150‑1 (MQ=255)
                                                                                                                                   cGGGATCGACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAg               <  2:724246/150‑1 (MQ=255)
                                                                                                                                    gggATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGt              <  1:2532820/150‑1 (MQ=255)
                                                                                                                                     ggATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGtt             <  1:2438290/150‑1 (MQ=255)
                                                                                                                                      gATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGttt            >  2:3827611/1‑150 (MQ=255)
                                                                                                                                      gATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGttt            >  2:2221879/1‑150 (MQ=255)
                                                                                                                                       aTCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCCAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACAAGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTc           >  2:792093/1‑150 (MQ=255)
                                                                                                                                        tCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCa          <  2:4651010/150‑1 (MQ=255)
                                                                                                                                          tACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAgg        <  1:2859216/150‑1 (MQ=255)
                                                                                                                                            cGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCg      <  1:2176273/150‑1 (MQ=255)
                                                                                                                                             ggTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGt     >  1:3301266/1‑150 (MQ=255)
                                                                                                                                                tACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAAt  >  2:4776510/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAAT  >  CP009273/4456086‑4456379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑