Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,457,499 C→A 100% R20S (CGT→AGT)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,457,4990CA100.0% 1141.5 / ‑6.7 347R20S (CGT→AGT) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (187/159);  minor base T (0/1);  total (187/160)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 347 total aligned reads displayed.

GTCCCTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAA  >  CP009273/4457352‑4457645
                                                                                                                                                   |                                                                                                                                                  
gTCCCTGCTCATCTTTATTACCTTCAGGTAAGTCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACACTGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGt                                                                                                                                                  >  2:4141101/1‑150 (MQ=255)
 tCCCTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTg                                                                                                                                                 <  1:1331296/150‑1 (MQ=255)
   ccTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGAt                                                                                                                                               >  1:2678333/1‑150 (MQ=255)
    cTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATc                                                                                                                                              <  1:1185486/150‑1 (MQ=255)
        tCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCAtt                                                                                                                                          <  1:4684117/150‑1 (MQ=255)
        tCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCAtt                                                                                                                                          <  2:2537562/150‑1 (MQ=255)
          aGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGc                                                                                                                                        >  1:73657/1‑150 (MQ=255)
          aGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGc                                                                                                                                        >  2:203435/1‑150 (MQ=255)
           gCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGcc                                                                                                                                       <  2:4807210/150‑1 (MQ=255)
            cTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCt                                                                                                                                      >  2:3462522/1‑150 (MQ=255)
                attaCCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGgcg                                                                                                                                  >  1:3360116/1‑150 (MQ=255)
                     cTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCgcag                                                                                                                             >  1:3225450/1‑150 (MQ=255)
                        cAGGTAAGGCTTCGCCACGCCTGAAGACAGTTCTGTACTGTGTCAGACAGGGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCCTTTACCTTCCCGTCTGGTTCAGAGTGATCCAGTGCCTGGCGCGcagcag                                                                                                                          >  2:329129/1‑150 (MQ=255)
                         aGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGa                                                                                                                         >  2:5076448/1‑150 (MQ=255)
                          ggTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGaa                                                                                                                        <  1:975466/150‑2 (MQ=255)
                          ggTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGAc                                                                                                                        >  1:4216476/1‑150 (MQ=255)
                           gTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACa                                                                                                                       <  1:2961042/150‑1 (MQ=255)
                           gTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACa                                                                                                                       <  1:461600/150‑1 (MQ=255)
                             aaGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGt                                                                                                                     >  2:1221918/1‑150 (MQ=255)
                             aaGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGt                                                                                                                     >  2:3225308/1‑150 (MQ=255)
                              aGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTg                                                                                                                    >  1:2640879/1‑150 (MQ=255)
                              aGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTg                                                                                                                    <  1:1461360/150‑1 (MQ=255)
                              aGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTg                                                                                                                    >  1:3742335/1‑150 (MQ=255)
                                 cTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAAt                                                                                                                 <  2:2752280/150‑1 (MQ=255)
                                  ttCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATa                                                                                                                <  2:2050100/150‑1 (MQ=255)
                                    cGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACg                                                                                                              <  2:1067427/150‑1 (MQ=255)
                                     gCCACGCCTGAGGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACgg                                                                                                             >  2:2221682/1‑150 (MQ=255)
                                     gCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACgg                                                                                                             <  2:694571/150‑1 (MQ=255)
                                        aCGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACggtgg                                                                                                          >  2:4170224/1‑150 (MQ=255)
                                            cTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTccc                                                                                                      <  1:386902/150‑1 (MQ=255)
                                            cTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTccc                                                                                                      <  2:217081/150‑1 (MQ=255)
                                              gAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCccgc                                                                                                    <  1:389312/150‑1 (MQ=255)
                                                  aCATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTc                                                                                                <  2:1264453/150‑1 (MQ=255)
                                                   cATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTcc                                                                                               <  2:2500750/150‑1 (MQ=255)
                                                     tttCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCtt                                                                                             >  2:4015347/1‑150 (MQ=255)
                                                       tCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTaa                                                                                           >  1:3766628/1‑150 (MQ=255)
                                                        cTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAg                                                                                          <  1:4538533/150‑1 (MQ=255)
                                                            aCTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTgcg                                                                                      >  1:1917/1‑150 (MQ=255)
                                                            aCTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTgcg                                                                                      >  1:1816/1‑150 (MQ=255)
                                                                tttCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAtt                                                                                  >  1:394502/1‑150 (MQ=255)
                                                                 ttCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTg                                                                                 <  2:3770896/150‑1 (MQ=255)
                                                                    aGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCt                                                                              >  1:4065940/1‑150 (MQ=255)
                                                                    aGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTGAAGTGCGCATTGCCt                                                                              >  1:1522706/1‑150 (MQ=255)
                                                                        aGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGaaa                                                                          >  2:3118519/1‑150 (MQ=255)
                                                                         gTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGaaaa                                                                         >  2:4487721/1‑150 (MQ=255)
                                                                          tGCGGAGGGACTCCTTATGTTTAAAGTATTTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCTGCAGACAGTGAATTCGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAAt                                                                        <  1:1423482/150‑1 (MQ=255)
                                                                           gCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATg                                                                       >  1:1059283/1‑150 (MQ=255)
                                                                             ggAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGc                                                                     <  1:675260/150‑1 (MQ=255)
                                                                              gAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCg                                                                    <  1:4264053/150‑1 (MQ=255)
                                                                                 ggACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTg                                                                 >  2:2974656/1‑150 (MQ=255)
                                                                                 ggACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGAACCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGAGGTg                                                                 >  2:3754204/1‑150 (MQ=255)
                                                                                  gACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGa                                                                >  1:4886835/1‑150 (MQ=255)
                                                                                   aCTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGAt                                                               >  2:2287176/1‑150 (MQ=255)
                                                                                     tCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGc                                                             >  1:1622567/1‑150 (MQ=255)
                                                                                       cTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGccc                                                           >  2:210830/1‑150 (MQ=255)
                                                                                       attATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCTCAGCAGACAGTGAATACTGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGccc                                                           <  2:5239833/149‑1 (MQ=255)
                                                                                             tttAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaagaa                                                     <  2:2243188/150‑1 (MQ=255)
                                                                                                  aGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAAtt                                                <  2:5199022/150‑1 (MQ=255)
                                                                                                   gAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGCGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATtg                                               <  2:1546281/150‑1 (MQ=255)
                                                                                                    aaaTTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATtgt                                              >  1:2204805/1‑150 (MQ=255)
                                                                                                    aaaTTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATtgt                                              >  1:865047/1‑150 (MQ=255)
                                                                                                    aaaTTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAGTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATtgt                                              <  2:3738211/150‑1 (MQ=255)
                                                                                                     aaTTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATtgtg                                             <  2:4065940/150‑1 (MQ=255)
                                                                                                      aTTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTgg                                            >  2:4673392/1‑150 (MQ=255)
                                                                                                        ttttACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGaa                                          <  2:496853/150‑1 (MQ=255)
                                                                                                          ttACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGaaaa                                        >  2:788764/1‑150 (MQ=255)
                                                                                                          ttACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGaaaa                                        >  2:2885971/1‑150 (MQ=255)
                                                                                                           tACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAAc                                       >  1:2808550/1‑150 (MQ=255)
                                                                                                           tACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAAc                                       <  1:896470/150‑1 (MQ=255)
                                                                                                            aCCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACg                                      >  1:1626198/1‑150 (MQ=255)
                                                                                                            aCCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACg                                      >  1:3103232/1‑150 (MQ=255)
                                                                                                            aCCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACg                                      <  1:5199471/150‑1 (MQ=255)
                                                                                                                 gCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGa                                 >  2:887111/1‑150 (MQ=255)
                                                                                                                  cTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGac                                >  1:2354164/1‑150 (MQ=255)
                                                                                                                  cTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGac                                >  1:2363236/1‑150 (MQ=255)
                                                                                                                   tCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGaca                               >  2:1101098/1‑150 (MQ=255)
                                                                                                                    cATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGacac                              <  1:1001120/150‑1 (MQ=255)
                                                                                                                    cATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGacac                              >  2:3983643/1‑150 (MQ=255)
                                                                                                                     aTTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAcc                             >  2:2312221/1‑150 (MQ=255)
                                                                                                                      ttCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAccc                            >  2:919626/1‑150 (MQ=255)
                                                                                                                        cGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCAt                          >  1:2110889/1‑150 (MQ=255)
                                                                                                                         gCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATc                         <  2:652788/150‑1 (MQ=255)
                                                                                                                          ccATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATcc                        >  2:1432045/1‑150 (MQ=255)
                                                                                                                           cATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCg                       <  1:4079444/150‑1 (MQ=255)
                                                                                                                             tttACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGa                     >  1:4447541/1‑150 (MQ=255)
                                                                                                                             tttACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGa                     >  2:2648661/1‑150 (MQ=255)
                                                                                                                              ttACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGaa                    >  2:844896/1‑150 (MQ=255)
                                                                                                                              ttACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGaa                    >  2:177190/1‑150 (MQ=255)
                                                                                                                               tACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAg                   <  1:4298892/150‑1 (MQ=255)
                                                                                                                                  cTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGt                <  2:1394340/150‑1 (MQ=255)
                                                                                                                                        gTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATc          >  2:4250424/1‑150 (MQ=255)
                                                                                                                                        gTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATc          <  1:5076448/150‑1 (MQ=255)
                                                                                                                                          cTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAg        <  1:3618051/150‑1 (MQ=255)
                                                                                                                                           tGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAgg       >  1:3485138/1‑150 (MQ=255)
                                                                                                                                            ggTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGc      >  2:2608256/1‑150 (MQ=255)
                                                                                                                                             gTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCg     >  2:5136411/1‑150 (MQ=255)
                                                                                                                                             gTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCg     <  2:3639735/150‑1 (MQ=255)
                                                                                                                                              ttCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCgg    >  2:4355371/1‑150 (MQ=255)
                                                                                                                                              ttCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCgg    >  1:25654/1‑150 (MQ=255)
                                                                                                                                                cATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGaa  >  1:5320947/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTCCCTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAA  >  CP009273/4457352‑4457645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑