Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,948 G→T 100% D503Y (GAT→TAT)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,9480GT99.3% 968.8 / ‑2.1 301D503Y (GAT→TAT) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (146/152);  total (146/155)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 304 total aligned reads displayed.

GTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAACC  >  CP009273/4458801‑4459094
                                                                                                                                                   |                                                                                                                                                  
gtggtAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTAt                                                                                                                                                  <  1:1006670/150‑1 (MQ=255)
gtggtAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTAt                                                                                                                                                  >  2:3666934/1‑150 (MQ=255)
  ggtAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATAc                                                                                                                                                >  1:4269087/1‑150 (MQ=255)
   gtAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACg                                                                                                                                               <  2:4862317/150‑1 (MQ=255)
      gTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTg                                                                                                                                            >  2:3265595/1‑150 (MQ=255)
      gTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTg                                                                                                                                            >  1:1954299/1‑150 (MQ=255)
          cAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATc                                                                                                                                        <  1:4569971/150‑1 (MQ=255)
           aGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCg                                                                                                                                       >  1:4556545/1‑150 (MQ=255)
           aGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCg                                                                                                                                       <  2:815236/150‑1 (MQ=255)
            gAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGt                                                                                                                                      >  2:5258445/1‑150 (MQ=255)
            gAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAAGGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGt                                                                                                                                      >  2:3393230/1‑150 (MQ=255)
              aaaTACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCa                                                                                                                                    <  2:1078133/150‑1 (MQ=255)
               aaTACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAg                                                                                                                                   <  1:1336169/150‑1 (MQ=255)
               aaTACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAg                                                                                                                                   >  1:5208330/1‑150 (MQ=255)
                  aCCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAgggg                                                                                                                                <  2:2260564/150‑1 (MQ=255)
                     gAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTg                                                                                                                             >  1:574415/1‑150 (MQ=255)
                     gAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTg                                                                                                                             >  2:2641931/1‑150 (MQ=255)
                     gAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTg                                                                                                                             >  2:3074558/1‑150 (MQ=255)
                      aGCACCCTCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTgc                                                                                                                            >  2:1623649/1‑150 (MQ=255)
                      aGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTgc                                                                                                                            <  1:3672777/150‑1 (MQ=255)
                       gCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTgcg                                                                                                                           >  1:279388/1‑150 (MQ=255)
                              cAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGtt                                                                                                                    <  2:1232707/150‑1 (MQ=255)
                               aGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTg                                                                                                                   >  2:3952651/1‑150 (MQ=255)
                                gCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGc                                                                                                                  >  1:2367032/1‑150 (MQ=255)
                                 cTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCg                                                                                                                 <  2:4365502/150‑1 (MQ=255)
                                 cTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCg                                                                                                                 >  1:3258809/1‑150 (MQ=255)
                                  tGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCgg                                                                                                                >  1:839707/1‑150 (MQ=255)
                                   ggTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGt                                                                                                               >  1:3948250/1‑150 (MQ=255)
                                    gTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTg                                                                                                              <  1:1170992/150‑1 (MQ=255)
                                       tGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGcg                                                                                                           <  2:477948/150‑1 (MQ=255)
                                          aaaGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGcgacg                                                                                                        >  1:1676934/1‑150 (MQ=255)
                                           aaGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGcgacga                                                                                                       >  1:1839030/1‑150 (MQ=255)
                                             ggTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGaaa                                                                                                     >  1:2221279/1‑150 (MQ=255)
                                                gCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATAc                                                                                                  >  2:1967479/1‑150 (MQ=255)
                                                   ttGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTg                                                                                               <  2:285671/150‑1 (MQ=255)
                                                   gtGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTg                                                                                               <  2:3720021/149‑1 (MQ=255)
                                                     gCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGcc                                                                                             <  1:4752303/150‑1 (MQ=255)
                                                      cAGGAAATCCTCTATGTGTGTTCGCAGGTGCGTCACAAGGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCa                                                                                            <  2:169415/150‑1 (MQ=255)
                                                      cAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGACa                                                                                            <  1:2132140/150‑1 (MQ=255)
                                                       aGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGAGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAg                                                                                           >  1:5056155/1‑150 (MQ=255)
                                                        ggAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAgc                                                                                          >  1:532231/1‑150 (MQ=255)
                                                         gAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGAGTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAgcg                                                                                         >  2:3341500/1‑150 (MQ=255)
                                                          aaaTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAgcgc                                                                                        >  1:1762068/1‑150 (MQ=255)
                                                          aaaTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAgcgc                                                                                        >  1:2284900/1‑150 (MQ=255)
                                                           aaTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGcgcg                                                                                       >  1:1365775/1‑150 (MQ=255)
                                                           aaTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGcgcg                                                                                       >  1:1451795/1‑150 (MQ=255)
                                                           aaTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGcgcg                                                                                       >  1:767211/1‑150 (MQ=255)
                                                              ccTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACACCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTCGCGACGAAATACCTGCCAGCGCGTGa                                                                                    <  2:2525215/150‑1 (MQ=255)
                                                                 cAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAgg                                                                                 >  1:4449099/1‑150 (MQ=255)
                                                                 cAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAgg                                                                                 <  2:5070584/150‑1 (MQ=255)
                                                                      tgtgtTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGAtt                                                                            <  1:290071/150‑1 (MQ=255)
                                                                       gtgtTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTa                                                                           >  1:2532989/1‑150 (MQ=255)
                                                                        tgtTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTAc                                                                          <  2:76306/150‑1 (MQ=255)
                                                                         gtTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTAcc                                                                         <  1:4359668/150‑1 (MQ=255)
                                                                               aGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcggg                                                                   >  1:2545180/1‑150 (MQ=255)
                                                                                   gCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGa                                                               <  2:206582/150‑1 (MQ=255)
                                                                                    cGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGAt                                                              >  1:2773631/1‑150 (MQ=255)
                                                                                    cGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGAGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGAt                                                              >  1:876927/1‑150 (MQ=255)
                                                                                       cacaATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGaa                                                           >  1:4059718/1‑150 (MQ=255)
                                                                                          aaTGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATcc                                                        <  1:360732/150‑1 (MQ=255)
                                                                                           ctGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCg                                                       <  2:4247859/149‑1 (MQ=255)
                                                                                               cGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCt                                                   <  1:3393230/150‑1 (MQ=255)
                                                                                                gagaTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTg                                                  <  2:842513/150‑1 (MQ=255)
                                                                                                 agaTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGa                                                 >  1:4838830/1‑150 (MQ=255)
                                                                                                  gaTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGAt                                                >  1:2614569/1‑150 (MQ=255)
                                                                                                    ttGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATcc                                              >  2:4817206/1‑150 (MQ=255)
                                                                                                      gtgCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTc                                            <  1:2657325/150‑1 (MQ=255)
                                                                                                        gCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGa                                          <  1:2163505/150‑1 (MQ=255)
                                                                                                         ccGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGaa                                         <  1:3167089/150‑1 (MQ=255)
                                                                                                          cGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAg                                        <  2:1867584/150‑1 (MQ=255)
                                                                                                          cGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAg                                        <  2:3556608/150‑1 (MQ=255)
                                                                                                          cGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAg                                        >  2:2170247/1‑150 (MQ=255)
                                                                                                          cGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAg                                        <  2:2221279/150‑1 (MQ=255)
                                                                                                           gCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAgg                                       <  1:975394/150‑1 (MQ=255)
                                                                                                           gCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAgg                                       <  1:3085324/150‑1 (MQ=255)
                                                                                                            cTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGa                                      <  2:1318685/150‑1 (MQ=255)
                                                                                                             tCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGat                                     <  1:1056734/150‑1 (MQ=255)
                                                                                                                 tGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGatatat                                 <  1:1737964/150‑1 (MQ=255)
                                                                                                                  gACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATAtt                                >  1:4773759/1‑150 (MQ=255)
                                                                                                                  gACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATAtt                                <  2:5163324/150‑1 (MQ=255)
                                                                                                                       catGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCttt                           <  2:2997099/150‑1 (MQ=255)
                                                                                                                       catGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCttt                           >  2:1914501/1‑150 (MQ=255)
                                                                                                                       catGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCttt                           <  2:3302880/150‑1 (MQ=255)
                                                                                                                       catGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCttt                           <  2:4556545/150‑1 (MQ=255)
                                                                                                                           ctgcGTAAGATTAAGCGGGTTACTTATTCGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCtt                       <  2:2707255/150‑1 (MQ=255)
                                                                                                                           ctgcGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCtt                       <  1:288601/150‑1 (MQ=255)
                                                                                                                           ctgcGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCtt                       <  2:595400/150‑1 (MQ=255)
                                                                                                                            tgcGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTg                      >  1:2837997/1‑150 (MQ=255)
                                                                                                                              cGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGAt                    >  2:4090174/1‑150 (MQ=255)
                                                                                                                               gTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATc                   >  1:3732742/1‑150 (MQ=255)
                                                                                                                               gTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATc                   <  1:3986108/150‑1 (MQ=255)
                                                                                                                                 aaGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCg                 >  2:2363681/1‑150 (MQ=255)
                                                                                                                                 aaGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCg                 <  1:2641931/150‑1 (MQ=255)
                                                                                                                                    aTTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCa              >  1:753705/1‑150 (MQ=255)
                                                                                                                                       aaGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCaaaa           >  1:2198511/1‑150 (MQ=255)
                                                                                                                                           gggTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAgaga       <  1:4215233/150‑1 (MQ=255)
                                                                                                                                             gTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACa     <  2:2738306/150‑1 (MQ=255)
                                                                                                                                                aCTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAAcc  <  1:3471012/150‑1 (MQ=255)
                                                                                                                                                aCTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAAcc  >  2:4754954/1‑150 (MQ=255)
                                                                                                                                                aCTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAAcc  <  1:3830316/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAACC  >  CP009273/4458801‑4459094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑