| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 360,173 | C→A | 6.4% | intergenic (‑107/+488) | lacZ ← / ← lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 360,173 | 0 | C | A | 6.4% | 720.9 / 6.7 | 253 | intergenic (‑107/+488) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (75/151); new base A (16/10); total (91/162) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.41e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 260 total aligned reads displayed.
CAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGAT > CP009273/360025‑360322 | ttcaccgacaaacaacagataaaccgacaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCGGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATcc > 2:3169126‑M1/80‑150 (MQ=255)cAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATcc < 2:34781/150‑1 (MQ=255) gggCTTACCAACCCGCGCCACCATCCTGTGCTGGAGCTCGTATTTTATGCTTACAAACATGAGAATTCCGACCCCCATTGCGGGGCTTTTTTGGGGGGGCGTCCGGCCCCCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCt < 1:912348/149‑1 (MQ=12) ggCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTc > 2:238792/1‑150 (MQ=255) ttACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCcg < 2:3238763/150‑1 (MQ=255) ttACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCcg < 2:4989502/150‑1 (MQ=255) caaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgtgttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGGTGCTTCGC‑AACGTTCAAATCCGCTCCcgg < 2:3954919‑M1/79‑1 (MQ=255) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCcggcg < 1:154895/150‑1 (MQ=255) ccATCCAGCGCCACCATCAAGTGCTGGAGCTCCGATGATAAGCTGTAAAACATGAGAATTCCGATCCCAAATTCCTGGAAGTTTATGGCGGGCTTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCcggcg < 1:118353/150‑1 (MQ=25) cATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCcggcgg < 2:2183217/150‑1 (MQ=255) aacagataaaacgaaaggcccagtctttcgactgagcctttcgttgtatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGGTGGTTCGC‑AACGGTATAAACCCCTccccgcggct < 1:5111563‑M1/84‑3 (MQ=255) acagataaaacgaaaggcccagtctttcgactgagccgttcgtgttatttgatgcctggggacttAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑CACGTGCAAATCcgcgcccggggggtg < 2:572289‑M1/85‑7 (MQ=255) agataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTg < 2:4799427‑M1/87‑1 (MQ=255) agataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTAAAATCCGCTCCCGGCGGATTTg < 2:463078‑M1/87‑1 (MQ=255) ataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTc < 2:1202572‑M1/89‑1 (MQ=255) cacccTCCCGGGCCGGAGCTCCGATGATACGCGGTCACCCATGAGAAGTCCGAGCCCCAATTCCTGGCAGTTTAGGGGGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTa < 1:2471156/150‑1 (MQ=17) caccaTCCAGTGCAGGAGCTCCGATGATACGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTTGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTa < 1:4358853/150‑1 (MQ=255) caccaTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑CACCGTAAAATCCGCGCCCGGCGGAGTTGtgcga > 2:1819403/1‑146 (MQ=25) caTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTc < 2:2034120/150‑1 (MQ=255) caTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTc > 1:3114405/1‑150 (MQ=255) aaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGGGCAAATCCGCTCCCGGCGGATTTGTCCTACTCAg < 2:5053077‑M1/97‑1 (MQ=255) ccAGTCCAGGCGCTCCGATGATCAGCTGTAAAACATGCGAATACCGATACCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCCgg < 2:2541272/150‑1 (MQ=18) aGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGCCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGgag < 1:5296397/150‑1 (MQ=255) gTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGACTTCCGATCCCCAATTCCTGGCTGTTTCTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGgaga < 1:120569/150‑1 (MQ=255) cccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGTTCGC‑AAAGTTCAAATACGCTCCCGGCGGATTTGTCCTACTCAGGagag < 1:682195‑M1/102‑1 (MQ=255) gCAGGAGCTCCGATGATAATCTGTCAAACATGAGAATTCCTATCCCAAATGCCTGGCAGTTTTTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGc < 2:1403416/150‑1 (MQ=18) cAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCg > 2:3738597/1‑150 (MQ=255) cAGGAGCGCCGAGGATAAGCTGTCAAACATGAGAATTCCGATCCCCACGTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCg < 2:2739679/150‑1 (MQ=255) ggAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGtt < 2:4153517/150‑1 (MQ=255) cGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGTTGGC‑AAAGGTGAAATCAGCGCCCCGCGGATTTGTGCAACACAGGAGAGCGTTCACCCac > 1:1992482/1‑150 (MQ=18) ctgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaa < 2:1335318‑M1/115‑1 (MQ=255) atAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGGTAGC‑AACGTTAAAATACGCTACCGGCGGATTTGTGCGAAACAGGGGTGGGGTAACCGacaaaac > 1:1598512/1‑148 (MQ=18) agcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaaca < 2:1142065‑M1/118‑1 (MQ=255) agcctttcgttttatgtgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCTTCGC‑AACGTTCAAATACCCTCCCGGCGGATATGTCCTAATCACGATATCGTTAACCCacaaaca < 1:864304‑M1/118‑1 (MQ=255) tAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGGTCGC‑AACGGTCAAATACGCTCCCGGCGGCTTTGTCCTACTAACGAGAGCGTTGACCGacaaaaaa > 1:1659660/1‑150 (MQ=18) cctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTTCTTCGC‑AACGTTAAAATACGCGCCCGGCGGATTTGTCCAACTCAGGAGAGCGTTAACCCACAaacaac < 2:2403197‑M1/120‑1 (MQ=255) atgCTGTCAAACCTGAGAATTCCGATCCCCAATTCCTGGCAGTTTAGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaacacc < 2:1045089/148‑3 (MQ=255) aGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAacaaca < 2:3396300/150‑1 (MQ=255) tttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAg < 1:2165190‑M1/122‑1 (MQ=255) ttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTAGC‑AACGTTCAAATCCGCTCCCGGCGGCTTTGTCCTACTCAGGAGAGCGTTAACCGACAAACAACAGa < 2:1469367‑M1/123‑1 (MQ=255) cgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGCTTTGTCCTACTCAGGAGAGCGTTCACCGACCAACAACagaaa < 2:4038159‑M1/125‑3 (MQ=255) cgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAACCAACAGATa < 2:3009600‑M1/125‑1 (MQ=255) cgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACCTTAAAATACGCTCCCCGCGGATTTGTCCTACTCATGTTAGCGTTGACCGACAAACAACAGATa < 1:110904‑M1/125‑1 (MQ=255) tCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaa < 2:1598715/150‑1 (MQ=255) ttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaa > 1:1338207‑M1/24‑150 (MQ=255) cAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACACACAACAGATaaa > 2:4854329/1‑150 (MQ=255) gttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCTTCGC‑AACGTTCAAATGCGCTCCCGGCGGAGTTGTCCGACTCAGGTGGGCGGTCACCGACCAACAACAGAtaaat < 1:4808848‑M1/128‑2 (MQ=255) aaCATGAGAATTCCGATCCCCAATTCCTGGCTGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAAc < 1:3644010/150‑1 (MQ=255) aaaTGTGAATGCCGATCCCCAATTCCTGGCTGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 2:4496535/148‑1 (MQ=255) aCATGAGAATTCCGATCCCCAATTCCTGGAAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 2:1895604/150‑1 (MQ=255) aTGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCGTCGC‑AACGGTCAAATCCGCGCCCGGCGGATTTGTCCTACACAGGAGAGCGTTCACCGACCAACAACACAAAAAACGaa > 1:2927538/1‑150 (MQ=14) ttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGaaa > 2:4390626‑M1/18‑150 (MQ=255) tGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGaaa < 2:1562703/150‑1 (MQ=255) tgatgcgtggggaattAATTCCTGGCTGTTTAGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg > 1:1486785‑M1/17‑150 (MQ=255) gagaATTCCGATCCCCAATTCCTGGCTTTTTTTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg < 2:1992482/150‑1 (MQ=255) gagaATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGGTGCGC‑ACCGGTCAAATACGCACCCGGCGGATTTGTGCGACTCAGGAGAGCGGTCACCGACAAAAAACAGATAAAACGAAAg > 1:4716824/1‑150 (MQ=14) gatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAg < 2:2687391‑M1/122‑1 (MQ=255) agaATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGGTCGC‑AACGGTCAAATCCGCTCCCGGCGGATTTGTCCTGCGCCGGAGTGCGGTGACCGACACACCACAGATAGATCGAAAgg > 1:3835466/1‑150 (MQ=14) atgcctggggaattATTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGc > 1:3726002‑M1/15‑150 (MQ=255) ttCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCa < 2:5190703/150‑1 (MQ=255) cctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCa > 1:1296677‑M1/12‑150 (MQ=255) tggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGt < 1:3075426‑M1/141‑1 (MQ=255) cGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGGTGGTTGGCAAAGGTACAATACCGAACCC‑GCGGTTTTGTGGTAATCAGGAGGGGGGTGACCCGCCAACACACGATAAAACGAAAGGGCCAGTc > 1:1907454/1‑150 (MQ=9) gggttttATTTCCTGGTTGTTTTTGGGGGGCGTCCTGCCCGCCACCCTCGGGGCCGTTGCTTCGC‑AACGTTCATTTCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt > 1:4799312‑M1/8‑150 (MQ=255) gATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCTTGGC‑AACGGTAAAATACGCTCCCGGCGGATTTGTCCAACGCAGGGGGGCGGTCACCGACAAACAACAGATAAAACGAAAGGGCCAGTca > 1:1779075/1‑149 (MQ=11) aTCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCtt < 2:539248/150‑1 (MQ=38) aTCCCCAATTCCTGGCAGTTTAGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCtt < 2:8017/150‑1 (MQ=38) ccccAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc < 1:3912691/150‑1 (MQ=35) ccccAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGGGTCGC‑AACGGTAAAATACGCGCCCGGCGGCGTTGTCCAACGCCGGAGAGCGGTCACCGACCAACAACAGATAAAACGAAAGGCCCCGTCTTTc > 1:3359928/1‑150 (MQ=11) ccccAATTCCGGGCTGTTTATGGGGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc < 1:2785839/150‑1 (MQ=255) cccAATTCCTGGCTGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg < 1:4859220/150‑1 (MQ=25) cccAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg < 1:5263319/150‑1 (MQ=35) cccAATTCCTGGCAGTTTAGGGCGGGCGTCCTGCCCGCCACACTCCGGGCCGTTGATTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg < 2:3884653/150‑1 (MQ=255) ccATTTCCTGGCTGTTTATGGGGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGa < 1:3202369/150‑1 (MQ=12) atttCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa < 1:5239714/126‑1 (MQ=14) aaTTCCTGGCAGTTTATGGGGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGAt < 1:1026072/84‑1 (MQ=25) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCcg < 1:4180340/78‑1 (MQ=35) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt > 2:5302409/1‑150 (MQ=32) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTCGC‑AAAGGTCAAAACCGCTCCCGGCGGTTTTGTCCGACACAGGAGAGCGTTCACCGACAAACAACAGATAAAAAGAAATGGCCAGTCattagtca > 1:2624346/1‑145 (MQ=11) aaTTCCTGGCAGTTTATGGAGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGAt > 2:1026072/1‑84 (MQ=25) cccGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGggga < 1:4739193/150‑1 (MQ=32) cGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCCGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat < 2:2498453/150‑1 (MQ=18) cGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat < 2:835760/150‑1 (MQ=33) gCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaatt < 1:4152344/150‑1 (MQ=34) aCCCTCCGGGCCGTTGCTTCGC‑AACGGTCAAATCCGCTCCCGGCGGATTTGTCCAACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTAGTCTGAGCCTTTCGTTTTATTTGTTGCCTGgggcattggg > 2:4232590/1‑150 (MQ=11) cTCCGGGCCGTTGCTTCGC‑AACGTTCAAATACGCTCCCGGCGGATTTGTCCTACACAGGAGAGCGTTCACCGACAAAAAACAGATAAAACGAAAGGCCCAGTCTTTAGACAGAGCCGTTCGTTTTATTTGATGCCTGgggaattaaatca > 2:477143/1‑144 (MQ=11) cGGGCCGTTGCTTCGC‑AAAGGTCAAATCCGCTCCCGGCGGATTTGTCCTAATCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCCGTCTTTCGACTGAGCCTTTCGTTTTATTTGAGGGCTGGGGTATTGGGcattaa > 2:3660719/1‑150 (MQ=11) ccGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCt < 1:565081/150‑1 (MQ=255) ccGTTGCGTCGC‑AACGTTCAAATCCGCGCCCGGCGGATTTGTCCTACTCCGGAGAGCGTTCACCGACACACCACAGATAAACCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCt < 2:5111563/150‑1 (MQ=14) gTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGACTTAATTCTTg > 1:2586129/1‑150 (MQ=255) tGCTTCCC‑AACGTACAAATCCGCACCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATACAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGaa < 1:4016623/150‑1 (MQ=255) aaCGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAAGTGGGAATTAATTCTTGAAGACGAAAg > 1:3268377/1‑150 (MQ=255) aCGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAACACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAgg < 2:864304/150‑1 (MQ=255) aCGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAgg > 2:1787234/1‑150 (MQ=255) ttCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGcc < 2:682195/150‑1 (MQ=255) ttCAAATCCGCTCCCGGCGGAGTTGTCCTACTCAGGAGAGCGGTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTGTCGTGTTCTTTGAGGCCTGGGGACTTGGGACTTAATTCTTGCAGACGACAGGGcc > 2:4777014/1‑150 (MQ=17) aaaTCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCGGGGGACTTGGGAATTAATTCTTGAAGACGAAAGGGCCTCg > 2:3048320/1‑150 (MQ=255) aTCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTg > 1:275061/1‑150 (MQ=255) aTCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTg > 1:3439281/1‑150 (MQ=255) aTCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTg > 1:4081456/1‑150 (MQ=255) | CAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGAT > CP009273/360025‑360322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |