Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,471,930 T→A 100% P575P (CCA→CCT)  valS ← valyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,471,9300TA98.2% 1051.0 / 4.8 324P575P (CCA→CCT) valSvalyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base A (150/168);  total (152/172)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 324 total aligned reads displayed.

GGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGAT  >  CP009273/4471783‑4472076
                                                                                                                                                   |                                                                                                                                                  
ggTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTTCCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAgg                                                                                                                                                  >  1:1354755/1‑150 (MQ=255)
ggTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAgg                                                                                                                                                  >  2:5114246/1‑150 (MQ=255)
ggTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAgg                                                                                                                                                  <  1:828467/150‑1 (MQ=255)
  tAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCa                                                                                                                                                <  1:4146464/150‑1 (MQ=255)
   aGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTTCGATTACGGATTTTGTCAGCCATCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAg                                                                                                                                               <  1:322994/150‑1 (MQ=255)
   aGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAg                                                                                                                                               >  1:3540728/1‑150 (MQ=255)
   aGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAg                                                                                                                                               >  1:597004/1‑150 (MQ=255)
     aCGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCg                                                                                                                                             >  2:4176978/1‑150 (MQ=255)
         cAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAAt                                                                                                                                         <  1:3363177/150‑1 (MQ=255)
         cAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAAt                                                                                                                                         >  2:4341496/1‑150 (MQ=255)
          aGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATa                                                                                                                                        <  1:2644229/150‑1 (MQ=255)
           gcgcCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATAc                                                                                                                                       <  1:5316530/150‑1 (MQ=255)
                  cAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAAc                                                                                                                                >  1:3502291/1‑150 (MQ=255)
                    gggTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCa                                                                                                                              <  1:4839109/150‑1 (MQ=255)
                     ggTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCat                                                                                                                             <  1:2015925/150‑1 (MQ=255)
                     ggTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCat                                                                                                                             >  2:3365968/1‑150 (MQ=255)
                        gAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATc                                                                                                                          <  2:124112/150‑1 (MQ=255)
                        gAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATc                                                                                                                          <  2:4165675/150‑1 (MQ=255)
                          agcgcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCa                                                                                                                        <  2:4220452/150‑1 (MQ=255)
                           gcgcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAg                                                                                                                       >  2:4041949/1‑150 (MQ=255)
                              cagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTgg                                                                                                                    >  1:2191827/1‑150 (MQ=255)
                               agcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTggg                                                                                                                   >  2:1816911/1‑150 (MQ=255)
                               agcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTggg                                                                                                                   <  2:2174494/150‑1 (MQ=255)
                                gcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGt                                                                                                                  <  1:3783490/150‑1 (MQ=255)
                                gcgcGTCAGTACCGTGCGGCTCAAGACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGt                                                                                                                  <  1:4335267/150‑1 (MQ=255)
                                 cgcgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTc                                                                                                                 <  1:5085917/150‑1 (MQ=255)
                                   cgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGTAGCGAAATACCGTCAACCATATCCAGTGGGTCGa                                                                                                               <  1:1001876/150‑1 (MQ=255)
                                   cgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGa                                                                                                               >  2:4380762/1‑150 (MQ=255)
                                    gTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGAt                                                                                                              >  2:1339925/1‑150 (MQ=255)
                                    gTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGAt                                                                                                              >  2:2498791/1‑150 (MQ=255)
                                      cAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATaa                                                                                                            >  1:4396764/1‑150 (MQ=255)
                                       aGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAAc                                                                                                           <  1:4645917/150‑1 (MQ=255)
                                       aGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAAc                                                                                                           >  2:3900370/1‑150 (MQ=255)
                                             gTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTAcc                                                                                                     >  1:4059988/1‑150 (MQ=255)
                                                cGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCtt                                                                                                  <  2:3935455/150‑1 (MQ=255)
                                                 ggCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTg                                                                                                 <  2:4491923/150‑1 (MQ=255)
                                                 ggCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTg                                                                                                 >  2:1155466/1‑150 (MQ=255)
                                                 ggCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTg                                                                                                 <  1:1333291/150‑1 (MQ=255)
                                                  gCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTgg                                                                                                >  2:2672148/1‑150 (MQ=255)
                                                    tCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAt                                                                                              <  2:4108512/150‑1 (MQ=255)
                                                     cAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAtt                                                                                             <  1:1416262/150‑1 (MQ=255)
                                                      aaTACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAttt                                                                                            >  2:3120285/1‑150 (MQ=255)
                                                       aTACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTg                                                                                           >  1:5190193/1‑150 (MQ=255)
                                                           cGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACa                                                                                       >  1:952168/1‑150 (MQ=255)
                                                             ttCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtc                                                                                     <  2:2811408/150‑1 (MQ=255)
                                                             ttCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtc                                                                                     >  2:1265599/1‑150 (MQ=255)
                                                                 ggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttct                                                                                 >  2:678930/1‑150 (MQ=255)
                                                                 ggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttct                                                                                 >  2:294464/1‑150 (MQ=255)
                                                                  gAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTg                                                                                >  2:2896543/1‑150 (MQ=255)
                                                                  gAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTg                                                                                >  1:1657575/1‑150 (MQ=255)
                                                                        cTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTc                                                                          <  2:4788294/150‑1 (MQ=255)
                                                                         ttCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCg                                                                         <  1:3801971/150‑1 (MQ=255)
                                                                           ctcGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtc                                                                       <  2:3869400/150‑1 (MQ=255)
                                                                            tcGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtca                                                                      >  1:1558178/1‑150 (MQ=255)
                                                                              ggTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACTTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcatc                                                                    <  1:3417716/150‑1 (MQ=255)
                                                                              ggTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcatc                                                                    <  1:2123157/150‑1 (MQ=255)
                                                                                 gcgcTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACg                                                                 <  2:1004116/150‑1 (MQ=255)
                                                                                    cTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAAt                                                              <  2:1654871/150‑1 (MQ=255)
                                                                                    cTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAAt                                                              <  2:230729/150‑1 (MQ=255)
                                                                                     ttACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATc                                                             <  1:295537/150‑1 (MQ=255)
                                                                                     ttACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATc                                                             >  2:1982644/1‑150 (MQ=255)
                                                                                     ttACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTAGTCATCACTAATc                                                             >  1:684834/1‑150 (MQ=255)
                                                                                        cGGATTTTGTCCGCCAGCTGCGGCTGCATCATAGTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAgg                                                          <  1:497072/150‑1 (MQ=255)
                                                                                           aTTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCg                                                       >  1:2387057/1‑150 (MQ=255)
                                                                                             tttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGt                                                     <  1:329268/150‑1 (MQ=255)
                                                                                              ttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTc                                                    >  1:3897655/1‑150 (MQ=255)
                                                                                                gTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCAt                                                  >  1:1098660/1‑150 (MQ=255)
                                                                                                gTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCAt                                                  >  1:2724058/1‑150 (MQ=255)
                                                                                                gTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCAt                                                  <  1:358639/150‑1 (MQ=255)
                                                                                                      cAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAAc                                            >  1:2743354/1‑150 (MQ=255)
                                                                                                             ggCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTgg                                     >  2:1600490/1‑150 (MQ=255)
                                                                                                             ggCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTgg                                     >  2:1325706/1‑150 (MQ=255)
                                                                                                              gCTGCATCATATTGCCGGTACGTTTTTCCTGCAGTTCAGGCTGCGTAAGTGCGTCATCCATATCCAGTGGGTCTATTACGTTACCCTTTTATTTGGTCATCTTCTTGCCTTCGTCATCACGAATCAGGCCGGTAATGTAAACGGTGTGGa                                    <  2:783044/150‑1 (MQ=255)
                                                                                                               cTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGaa                                   >  1:3795731/1‑150 (MQ=255)
                                                                                                                     catATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCAc                             >  1:2288390/1‑150 (MQ=255)
                                                                                                                      atatTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCAACACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCAcc                            >  1:4003120/1‑150 (MQ=255)
                                                                                                                       tatTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCt                           >  2:3630500/1‑150 (MQ=255)
                                                                                                                       tatTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCt                           <  2:139965/150‑1 (MQ=255)
                                                                                                                        atTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTg                          >  2:899332/1‑150 (MQ=255)
                                                                                                                        atTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTg                          >  2:5150575/1‑150 (MQ=255)
                                                                                                                         ttGACGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGTTCGATAACGTTACCCTTGGTTTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTTAACGGTGTGGAACGGCACCTGc                         <  2:5278450/150‑1 (MQ=255)
                                                                                                                          tGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCg                        <  2:863056/150‑1 (MQ=255)
                                                                                                                                  cGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCa                >  1:263225/1‑150 (MQ=255)
                                                                                                                                   gTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt               >  1:4696047/1‑150 (MQ=255)
                                                                                                                                   gTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt               <  1:3304212/150‑1 (MQ=255)
                                                                                                                                   gTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt               >  1:3256668/1‑150 (MQ=255)
                                                                                                                                   gTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt               >  1:1241581/1‑150 (MQ=255)
                                                                                                                                   gTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt               >  2:4597360/1‑150 (MQ=255)
                                                                                                                                      tttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAtttt            >  2:826426/1‑150 (MQ=255)
                                                                                                                                      tttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAtttt            <  1:4724900/150‑1 (MQ=255)
                                                                                                                                       ttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTc           >  1:3104140/1‑150 (MQ=255)
                                                                                                                                       ttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTc           <  1:2836034/150‑1 (MQ=255)
                                                                                                                                       ttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTc           <  2:2439322/150‑1 (MQ=255)
                                                                                                                                       ttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTc           <  2:3726003/150‑1 (MQ=255)
                                                                                                                                        tCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCa          <  2:1450311/150‑1 (MQ=255)
                                                                                                                                         ccAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCAt         <  2:4709772/150‑1 (MQ=255)
                                                                                                                                           agcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCt       >  2:2548928/1‑150 (MQ=255)
                                                                                                                                            gcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCtt      <  2:4932912/150‑1 (MQ=255)
                                                                                                                                              agTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTtg    <  1:4454763/150‑1 (MQ=255)
                                                                                                                                                ttCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTtgat  <  1:1786768/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGAT  >  CP009273/4471783‑4472076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑