BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,495,463	G→T	6.8%	M120I (ATG→ATT)	yjhC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,495,463	0	G	T	6.8%	1430.8 / 41.2	395	M120I (ATG→ATT)	yjhC	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (190/176); new base T (17/12); total (207/188)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

TATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACA  >  CP009273/4495314‑4495612

tATCTGCATAAAGCACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATg                                                                                                                                                       <  1:3714584/150‑1 (MQ=255)

tATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATg                                                                                                                                                       >  1:1204629/1‑150 (MQ=255)

   cTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAAt                                                                                                                                                    <  2:2664844/150‑1 (MQ=255)

   cTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAAt                                                                                                                                                    <  2:2854885/150‑1 (MQ=255)

        tAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAAtttttt                                                                                                                                               >  2:292436/1‑150 (MQ=255)

         aaaGAACCATTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTc                                                                                                                                              <  1:3126729/150‑1 (MQ=255)

           aGAACCAGTAATTAAAGCAGCACAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCaa                                                                                                                                            >  2:4661171/1‑150 (MQ=255)

            gAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAAt                                                                                                                                           <  1:47521/150‑1 (MQ=255)

              aCCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATgg                                                                                                                                         >  1:2788499/1‑150 (MQ=255)

               ccAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATggg                                                                                                                                        <  1:1655370/150‑1 (MQ=255)

               ccAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATggg                                                                                                                                        <  2:1736766/150‑1 (MQ=255)

               ccAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATggg                                                                                                                                        <  2:5035433/150‑1 (MQ=255)

                 aGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGt                                                                                                                                      <  1:1054459/150‑1 (MQ=255)

                 aGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGt                                                                                                                                      <  2:551366/150‑1 (MQ=255)

                  gTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGtt                                                                                                                                     <  1:2166739/150‑1 (MQ=255)

                   tAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTc                                                                                                                                    <  1:1885761/150‑1 (MQ=255)

                     aTTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCaa                                                                                                                                  <  2:1704571/150‑1 (MQ=255)

                      ttAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAat                                                                                                                                 <  2:2152940/150‑1 (MQ=255)

                      ttAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAat                                                                                                                                 <  1:831842/150‑1 (MQ=255)

                      ttAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAat                                                                                                                                 >  2:4031549/1‑150 (MQ=255)

                        aaaGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAatat                                                                                                                               <  2:1439995/150‑1 (MQ=255)

                         aaGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATg                                                                                                                              >  1:764648/1‑150 (MQ=255)

                          agcagcAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGc                                                                                                                             >  1:1664472/1‑150 (MQ=255)

                            cagcaAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATTAATTTTTTCAATGGGGTTCAATATGCAc                                                                                                                           >  2:3298883/1‑150 (MQ=255)

                             agcaAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACg                                                                                                                          <  1:1796745/150‑1 (MQ=255)

                                aaaGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGaa                                                                                                                       >  2:3488325/1‑150 (MQ=255)

                                aaaGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGaa                                                                                                                       >  2:207960/1‑150 (MQ=255)

                                 aaGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAg                                                                                                                      <  2:1136437/150‑1 (MQ=255)

                                   gAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGtt                                                                                                                    >  2:617615/1‑150 (MQ=255)

                                   gAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGtt                                                                                                                    <  1:4678361/150‑1 (MQ=255)

                                     aTAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGttaa                                                                                                                  >  2:3646824/1‑150 (MQ=255)

                                       aagaagCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGAGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGttaatt                                                                                                                >  2:4496746/1‑150 (MQ=255)

                                        agaagCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGttaatta                                                                                                               >  1:2320015/1‑150 (MQ=255)

                                          aagCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTaaa                                                                                                             >  1:4594317/1‑150 (MQ=255)

                                           agCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATTAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAaag                                                                                                            <  1:147628/150‑1 (MQ=255)

                                            gCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAaaga                                                                                                           <  2:4917675/150‑1 (MQ=255)

                                             cATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCCATGGGGTTCAATATGCACGGAAGTTAATTAaagaa                                                                                                          >  2:3008542/1‑150 (MQ=255)

                                              aTGTTTTTTGTGAAAAACAAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAaagaag                                                                                                         <  2:3982235/150‑1 (MQ=255)

                                                gTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGgt                                                                                                       <  2:2491760/150‑1 (MQ=255)

                                                      tgtgAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATc                                                                                                 >  1:4567147/1‑150 (MQ=255)

                                                       gtgAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCg                                                                                                <  1:2921588/150‑1 (MQ=255)

                                                       gtgAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCg                                                                                                <  1:4037803/150‑1 (MQ=255)

                                                          aaaaaCCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCg                                                                                             >  2:3876184/1‑150 (MQ=255)

                                                          aaaaaCCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCg                                                                                             <  2:1322378/150‑1 (MQ=255)

                                                            aaaCCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCT‑GTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGaaa                                                                                          >  2:4113753/1‑150 (MQ=255)

                                                              aCCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAat                                                                                         >  2:1502680/1‑150 (MQ=255)

                                                                cAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAatat                                                                                       <  2:1043690/150‑1 (MQ=255)

                                                                cAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAatat                                                                                       <  2:1210547/150‑1 (MQ=255)

                                                                cAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGATGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAatat                                                                                       >  2:4191122/1‑150 (MQ=255)

                                                                 aaTTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAAtatt                                                                                      >  1:3522082/1‑150 (MQ=255)

                                                                 aaTTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAAtatt                                                                                      >  1:5318821/1‑150 (MQ=255)

                                                                    tGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATc                                                                                   <  1:4159624/150‑1 (MQ=255)

                                                                      cATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCAt                                                                                 >  2:4368722/1‑150 (MQ=255)

                                                                      cATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCAt                                                                                 >  1:1401425/1‑150 (MQ=255)

                                                                      cATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCAt                                                                                 <  2:771060/150‑1 (MQ=255)

                                                                        ttAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGt                                                                               <  1:2582984/150‑1 (MQ=255)

                                                                         tAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATTAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTc                                                                              >  1:2447839/1‑150 (MQ=255)

                                                                                 gtaGATTGTGTGGTTATGGTCTTAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTGTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGTAATATTATCATGTCATACTAag                                                                      <  1:3055728/148‑1 (MQ=255)

                                                                                  aaGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAaga                                                                     >  2:1809910/1‑150 (MQ=255)

                                                                                   aGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAagag                                                                    >  1:4809800/1‑150 (MQ=255)

                                                                                     aTTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGaa                                                                  <  1:3241861/150‑1 (MQ=255)

                                                                                     aTTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGaa                                                                  >  2:2436761/1‑150 (MQ=255)

                                                                                     aTTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGaa                                                                  >  1:5200009/1‑150 (MQ=255)

                                                                                      ttGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGaaa                                                                 <  1:2717433/150‑1 (MQ=255)

                                                                                            ggATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATTAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTg                                                           >  2:2006194/1‑150 (MQ=255)

                                                                                              atatGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTggg                                                         <  1:658821/150‑1 (MQ=255)

                                                                                              atatGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTggg                                                         >  2:5232859/1‑150 (MQ=255)

                                                                                              atatGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTggg                                                         >  1:4282709/1‑150 (MQ=255)

                                                                                              atatGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTggg                                                         <  1:3913248/150‑1 (MQ=255)

                                                                                              atatGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGAATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTggg                                                         <  1:1591207/150‑1 (MQ=255)

                                                                                                atGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGaa                                                       >  2:4701947/1‑150 (MQ=255)

                                                                                                  ggTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAATGAAGGTGTTATCGGCGAAATATTATCATTTCATACTAAGAGAAATGGCTGGGaaaa                                                     >  2:3249634/1‑150 (MQ=255)

                                                                                                   gTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAaaac                                                    >  1:5081110/1‑150 (MQ=255)

                                                                                                          cGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATTAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAag                                             <  1:1348804/150‑1 (MQ=255)

                                                                                                          cGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGACATGGCTGGGAAAACAAACAag                                             >  2:4267710/1‑150 (MQ=255)

                                                                                                            tgtAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAagag                                           <  2:2559293/150‑1 (MQ=255)

                                                                                                              tAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAagagag                                         <  2:2775253/150‑1 (MQ=255)

                                                                                                               aaaGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATTAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAgagaga                                        >  1:1531099/1‑150 (MQ=255)

                                                                                                                 agaagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATGAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACt                                      <  1:2848442/150‑1 (MQ=255)

                                                                                                                   aagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACttt                                    <  1:5235006/150‑1 (MQ=255)

                                                                                                                   aagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACttt                                    >  1:2497899/1‑150 (MQ=255)

                                                                                                                          tgtgACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGaa                             <  1:4360933/150‑1 (MQ=255)

                                                                                                                           gtgACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGaaa                            >  1:2640499/1‑150 (MQ=255)

                                                                                                                              aCCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAg                         >  2:4744657/1‑150 (MQ=255)

                                                                                                                                 tttATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATg                      <  1:4191122/150‑1 (MQ=255)

                                                                                                                                 tttATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATg                      >  1:1989942/1‑150 (MQ=255)

                                                                                                                                      ggCCGGGCATATTATTAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGa                 <  2:1553118/150‑1 (MQ=255)

                                                                                                                                      ggCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGa                 >  2:2344612/1‑150 (MQ=255)

                                                                                                                                        ccGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAAc               <  1:207960/150‑1 (MQ=255)

                                                                                                                                         cGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTAATACTAAGAGAAATGTCTGGGAAAACAAACAAGAGAGAATTTCCTGGAAAAAGATGAAAGaaaa              >  1:2366985/1‑148 (MQ=255)

                                                                                                                                           ggCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAAt            <  2:2241401/150‑1 (MQ=255)

                                                                                                                                           ggCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAAt            >  2:5180057/1‑150 (MQ=255)

                                                                                                                                           ggCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAAt            <  2:3133138/150‑1 (MQ=255)

                                                                                                                                            gCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATc           <  1:3488325/150‑1 (MQ=255)

                                                                                                                                            gCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATc           <  1:4981476/150‑1 (MQ=255)

                                                                                                                                              atatTATGAATTGTTTAAATGGGGTTCAATATGCAAGGAAGTTAATTAAAGAAGGAGTTATCGGCGAATTATTATCATGTCATACTAATAGAAATGGCTGTGAAAACAAAATATATATACTTTCCTGGAAAAAGATGAAAGAACAATCtg         >  2:2325178/1‑150 (MQ=255)

                                                                                                                                               tattatTAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtgg        >  1:4441673/1‑150 (MQ=255)

                                                                                                                                               tattatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtgg        >  1:336839/1‑150 (MQ=255)

                                                                                                                                               tattatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtgg        <  1:4470724/150‑1 (MQ=255)

                                                                                                                                                attattAATTTTTTCAATGGTGTTCAATATGCACGGAAGTTAATTTAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtggt       <  2:749568/150‑1 (MQ=255)

TATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACA  >  CP009273/4495314‑4495612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`