BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,513,759	T→A	6.0%	G51G (GGA→GGT)	yjhG ←	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,513,759	0	T	A	6.0%	900.3 / 29.1	293	G51G (GGA→GGT)	yjhG	putative dehydratase
Reads supporting (aligned to +/- strand): ref base T (132/143); new base A (7/11); total (139/154)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

TCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAATATTGCGAACAG  >  CP009273/4513610‑4513907

  ggCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTcc                                                                                                                                                    <  2:1320350/150‑1 (MQ=255)

  ggCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCAAGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTcc                                                                                                                                                    <  2:2583477/150‑1 (MQ=255)

   gccgccGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCg                                                                                                                                                   <  1:4119727/150‑1 (MQ=255)

     cgccgcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGc                                                                                                                                                 <  2:3886908/150‑1 (MQ=255)

     cgccgcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGc                                                                                                                                                 >  2:3141915/1‑150 (MQ=255)

       ccgcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAt                                                                                                                                               >  2:4206788/1‑150 (MQ=255)

        cgcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAtt                                                                                                                                              <  2:4997543/150‑1 (MQ=255)

        cgcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAtt                                                                                                                                              >  2:416228/1‑150 (MQ=255)

        cgcTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAtt                                                                                                                                              <  2:3726388/150‑1 (MQ=255)

          cTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCa                                                                                                                                            >  1:3814196/1‑150 (MQ=255)

           tttCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCAt                                                                                                                                           <  2:1188480/150‑1 (MQ=255)

           tttCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATACCGGCATTCAt                                                                                                                                           >  2:3816450/1‑150 (MQ=255)

             tCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATgg                                                                                                                                         <  2:3307454/150‑1 (MQ=255)

               aTCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTc                                                                                                                                       <  2:2182375/150‑1 (MQ=255)

               aTCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTc                                                                                                                                       <  1:4604428/150‑1 (MQ=255)

                tCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCa                                                                                                                                      >  1:2693277/1‑150 (MQ=255)

                 cTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCAt                                                                                                                                     <  1:1695714/150‑1 (MQ=255)

                 cTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCAt                                                                                                                                     >  1:1301341/1‑150 (MQ=255)

                   ggATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATAc                                                                                                                                   >  1:2349183/1‑150 (MQ=255)

                    gATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATAcc                                                                                                                                  <  2:2766362/150‑1 (MQ=255)

                     aTGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCg                                                                                                                                 >  1:2481150/1‑150 (MQ=255)

                       gTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGaa                                                                                                                               >  2:4679086/1‑150 (MQ=255)

                         ccAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACa                                                                                                                             >  2:748461/1‑150 (MQ=255)

                          cAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAATCATGGTCATACCGAACAg                                                                                                                            >  2:3035414/1‑150 (MQ=255)

                             ttCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATc                                                                                                                         <  1:3508806/150‑1 (MQ=255)

                                gTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCTCCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATcccc                                                                                                                      <  1:1884774/150‑1 (MQ=255)

                                gTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATcccc                                                                                                                      <  1:214840/150‑1 (MQ=255)

                                  aaTGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGc                                                                                                                    >  2:1107618/1‑150 (MQ=255)

                                    tGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTc                                                                                                                  <  2:2956537/150‑1 (MQ=255)

                                     gCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCg                                                                                                                 <  2:1471840/150‑1 (MQ=255)

                                       cccTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCggg                                                                                                               <  2:1853043/150‑1 (MQ=255)

                                        ccTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGc                                                                                                              <  1:3911549/150‑1 (MQ=255)

                                         cTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCg                                                                                                             <  2:503806/150‑1 (MQ=255)

                                           ggTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGt                                                                                                           >  2:5051622/1‑150 (MQ=255)

                                            gTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGtt                                                                                                          <  2:3358972/150‑1 (MQ=255)

                                             tGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTg                                                                                                         >  1:4954338/1‑150 (MQ=255)

                                             tGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTg                                                                                                         >  1:4895158/1‑150 (MQ=255)

                                               cAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGAt                                                                                                       >  2:2336398/1‑150 (MQ=255)

                                               cAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGAt                                                                                                       <  2:2492476/150‑1 (MQ=255)

                                               cAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGAt                                                                                                       >  1:3821637/1‑150 (MQ=255)

                                                aaCGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATa                                                                                                      >  1:4190133/1‑150 (MQ=255)

                                                 aCGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATaa                                                                                                     >  1:2276783/1‑150 (MQ=255)

                                                      aGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCAtc                                                                                                >  1:3578704/1‑150 (MQ=255)

                                                         gcCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATctct                                                                                             <  2:5275640/150‑1 (MQ=255)

                                                              gggTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGt                                                                                        >  2:3670248/1‑150 (MQ=255)

                                                              gggTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAAACCATTCCGGCATTCATGGTCATAACGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGt                                                                                        >  2:4440279/1‑150 (MQ=255)

                                                                gTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTa                                                                                      <  1:2842579/150‑1 (MQ=255)

                                                                  ttACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAAt                                                                                    >  2:3375730/1‑150 (MQ=255)

                                                                      cGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGa                                                                                >  1:2975143/1‑150 (MQ=255)

                                                                        tCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGt                                                                              <  2:609766/150‑1 (MQ=255)

                                                                          tGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTc                                                                            <  2:1707179/150‑1 (MQ=255)

                                                                          tGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGAACCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTc                                                                            <  2:1431877/150‑1 (MQ=255)

                                                                           gcgcCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCg                                                                           >  1:4378531/1‑150 (MQ=255)

                                                                               cgcgTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGt                                                                       >  2:4975790/1‑150 (MQ=255)

                                                                                 cgTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTcc                                                                     >  2:3842733/1‑150 (MQ=255)

                                                                                  gTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCg                                                                    >  1:101960/1‑150 (MQ=255)

                                                                                    aaGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCggg                                                                  >  2:3504251/1‑150 (MQ=255)

                                                                                      gCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGcc                                                                <  1:395024/150‑1 (MQ=255)

                                                                                       ccACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCa                                                               <  1:1471578/150‑1 (MQ=255)

                                                                                            gAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATACCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGgc                                                          >  1:2929667/1‑150 (MQ=255)

                                                                                               tgtACTGAGCAGTAAACTACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCCTACCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATACGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGgcgtg                                                       <  1:1109701/150‑1 (MQ=255)

                                                                                                 tACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCAGTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATACGCATCTCTGCGGTTACTGGGAGTTCGCCGACCGGGCCATCGgcgtgcg                                                     >  2:4499218/1‑150 (MQ=255)

                                                                                                 tACTGAGCAGTAAAATAACGTCCCGATCCAGCTAGTCCGGAGACAAAACCATTCCGTCATTCATGGTCATACCGAACAGATCACCGCTCGGGAGGTTGATAAGCATCTCTTCGGTTAATGGGAGTTCGCCGTCCGGTCCATCGgcgtgcg                                                     >  1:4500570/1‑150 (MQ=255)

                                                                                                    tGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTc                                                  <  1:1267944/150‑1 (MQ=255)

                                                                                                     gAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCt                                                 <  1:2211398/150‑1 (MQ=255)

                                                                                                     gAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCt                                                 <  1:3625069/150‑1 (MQ=255)

                                                                                                      aGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTg                                                >  1:611108/1‑150 (MQ=255)

                                                                                                        cAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGAc                                              <  2:659039/150‑1 (MQ=255)

                                                                                                         aGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACg                                             >  2:5213777/1‑150 (MQ=255)

                                                                                                         aGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACg                                             >  2:3996975/1‑150 (MQ=255)

                                                                                                            aaaaTACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGgtg                                          <  1:891070/150‑1 (MQ=255)

                                                                                                            aaaaTACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGgtg                                          <  2:127310/150‑1 (MQ=255)

                                                                                                             aaaTACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATACCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGgtgt                                         >  1:3117321/1‑150 (MQ=255)

                                                                                                              aaTACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTa                                        >  1:3373817/1‑150 (MQ=255)

                                                                                                                 aCCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAat                                     <  1:1047171/150‑1 (MQ=255)

                                                                                                                 aCCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAat                                     >  1:1746147/1‑150 (MQ=255)

                                                                                                                  ccGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAata                                    <  1:1416126/150‑1 (MQ=255)

                                                                                                                   cGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGTTTGATAAGCATCTCTGCGGTTAATGGGAGATAGCCGTCCGCGCCATCGTCGTGCGTTCTGACTGTGTAAatat                                   >  2:3592801/1‑150 (MQ=255)

                                                                                                                   cGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAatat                                   <  1:1251512/150‑1 (MQ=255)

                                                                                                                   cGTCCCGATCCAGCTCGTCCGGAGACCAACCCATACCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAatat                                   <  1:2592338/150‑1 (MQ=255)

                                                                                                                    gTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATc                                  >  1:3316213/1‑150 (MQ=255)

                                                                                                                     tCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCg                                 >  1:555998/1‑150 (MQ=255)

                                                                                                                        cGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTgg                              <  1:2070307/150‑1 (MQ=255)

                                                                                                                         gATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGc                             >  1:720877/1‑150 (MQ=255)

                                                                                                                         gATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGc                             <  1:4467849/150‑1 (MQ=255)

                                                                                                                            ccAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGctct                          >  2:576616/1‑150 (MQ=255)

                                                                                                                             cAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCtctc                         <  2:5084621/150‑1 (MQ=255)

                                                                                                                              aGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCg                        >  1:5053028/1‑150 (MQ=255)

                                                                                                                                 tCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATCCCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCa                     <  2:1797493/150‑1 (MQ=255)

                                                                                                                                  cGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAg                    <  2:161373/150‑1 (MQ=255)

                                                                                                                                  cGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAg                    <  2:2075032/150‑1 (MQ=255)

                                                                                                                                       ggAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCaaaa               >  1:799070/1‑150 (MQ=255)

                                                                                                                                       ggAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCaaaa               >  2:2458745/1‑150 (MQ=255)

                                                                                                                                         agaCCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAat             <  2:1596510/150‑1 (MQ=255)

                                                                                                                                             cAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAATATTg         >  1:1624905/1‑150 (MQ=255)

                                                                                                                                               aCCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAATATTGCg       <  1:3756282/150‑1 (MQ=255)

                                                                                                                                               aCCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAATATTGCg       <  1:4651457/150‑1 (MQ=255)

                                                                                                                                                  cATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAATATTGCGAAc    <  1:3597485/150‑1 (MQ=255)

                                                                                                                                                   aTTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAATATTGCGAACa   >  2:1916683/1‑150 (MQ=255)

                                                                                                                                                    ttCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAATATTGCGAACAg  <  2:1413004/150‑1 (MQ=255)

TCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTGATAAGCATCTCTGCGGTTAATGGGAGTTCGCCGTCCGGGCCATCGGCGTGCGTTCTGACGGTGTAAATATCGTGGCTCTCGTCAGCAAAAATATTGCGAACAG  >  CP009273/4513610‑4513907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`