Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,531,143 A→T 100% I124F (ATT→TTT)  fimB → tyrosine recombinase/inversion of on/off regulator of fimA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,531,1430AT100.0% 1195.9 / ‑6.7 371I124F (ATT→TTT) fimBtyrosine recombinase/inversion of on/off regulator of fimA
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base T (187/180);  total (188/183)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 374 total aligned reads displayed.

TTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCAT  >  CP009273/4530996‑4531289
                                                                                                                                                   |                                                                                                                                                  
ttttCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAtttt                                                                                                                                                  <  2:3125661/150‑1 (MQ=255)
     aacaacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTAtctc                                                                                                                                             >  2:3408734/1‑150 (MQ=255)
       caacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGa                                                                                                                                           <  1:4532528/150‑1 (MQ=255)
       caacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGa                                                                                                                                           >  1:3281794/1‑150 (MQ=255)
       caacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGa                                                                                                                                           >  1:1436316/1‑150 (MQ=255)
           gCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCATTCTCGGCAACAGTTTTAACATTTTATCTCGACTTc                                                                                                                                       >  1:635756/1‑150 (MQ=255)
            cACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTcc                                                                                                                                      <  2:1718109/150‑1 (MQ=255)
            cACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTcc                                                                                                                                      >  1:2282570/1‑150 (MQ=255)
             aCCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCg                                                                                                                                     >  1:3161949/1‑150 (MQ=255)
             aCCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCg                                                                                                                                     >  1:935410/1‑150 (MQ=255)
              cccGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCgg                                                                                                                                    <  1:3096940/150‑1 (MQ=255)
               ccGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggt                                                                                                                                   >  2:4552995/1‑150 (MQ=255)
                 gCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtgg                                                                                                                                 <  1:4138339/150‑1 (MQ=255)
                  cTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtggt                                                                                                                                <  1:1764301/150‑1 (MQ=255)
                    aTTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTaa                                                                                                                              >  1:1444513/1‑150 (MQ=255)
                    aTTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGAATTCCGGTGGTaa                                                                                                                              >  1:2338953/1‑150 (MQ=255)
                     ttGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAAt                                                                                                                             <  2:3218237/150‑1 (MQ=255)
                     ttGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAAt                                                                                                                             <  1:1726807/150‑1 (MQ=255)
                        aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGcc                                                                                                                          >  1:4636332/1‑150 (MQ=255)
                        aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGcc                                                                                                                          >  2:1756090/1‑150 (MQ=255)
                        aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGcc                                                                                                                          >  1:3218878/1‑150 (MQ=255)
                        aaTAAAGAAGTTCAGGCTTTAAAAAACAGGTTGAGTAACCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTACGGGGAATCCGCGTTCTCGGCACCAGTTTTACCATTTTATCTCGACTTCCGGTGGTAAGGcc                                                                                                                          >  1:278023/1‑150 (MQ=255)
                           aaaGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCggg                                                                                                                       >  2:5126829/1‑150 (MQ=255)
                           aaaGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCggg                                                                                                                       >  2:574627/1‑150 (MQ=255)
                            aagaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAATGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTAGACTTCCGGTGGTAATGCCGGGt                                                                                                                      >  1:2061272/1‑150 (MQ=255)
                             agaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTTATGCCGGGtt                                                                                                                     <  2:762877/150‑1 (MQ=255)
                             agaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAAGGCCGGGtt                                                                                                                     <  2:1893495/150‑1 (MQ=255)
                                agTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTc                                                                                                                  >  1:2751549/1‑150 (MQ=255)
                                agTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTc                                                                                                                  >  2:3521043/1‑150 (MQ=255)
                                agTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTc                                                                                                                  >  1:4351362/1‑150 (MQ=255)
                                agTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTc                                                                                                                  >  1:5175730/1‑150 (MQ=255)
                                   tgatgCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACt                                                                                                               <  1:1816140/146‑1 (MQ=255)
                                    cAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTg                                                                                                              >  1:3629036/1‑150 (MQ=255)
                                       gCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGgag                                                                                                           >  1:3704148/1‑150 (MQ=255)
                                          ttAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGAtt                                                                                                        <  1:870878/150‑1 (MQ=255)
                                            aaaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCa                                                                                                      <  2:2609876/150‑1 (MQ=255)
                                            aaaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCa                                                                                                      >  2:958937/1‑150 (MQ=255)
                                                         aGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                         >  2:3900834/1‑150 (MQ=255)
                                                         aGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                         <  2:36671/150‑1 (MQ=255)
                                                          gTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTAc                                                                                        >  1:1533669/1‑150 (MQ=255)
                                                          gTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTAc                                                                                        <  1:5002336/150‑1 (MQ=255)
                                                          gTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTAc                                                                                        <  2:2750163/150‑1 (MQ=255)
                                                           tATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACg                                                                                       <  1:1021760/150‑1 (MQ=255)
                                                            aTCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGc                                                                                      >  1:4046103/1‑150 (MQ=255)
                                                               cGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCAt                                                                                   >  2:3005915/1‑150 (MQ=255)
                                                                gTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCAtt                                                                                  <  1:3527462/150‑1 (MQ=255)
                                                                gTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCAtt                                                                                  >  2:2820306/1‑150 (MQ=255)
                                                                  aCTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCg                                                                                >  1:5295062/1‑150 (MQ=255)
                                                                    ttCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgtg                                                                              <  2:618685/150‑1 (MQ=255)
                                                                      cGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGtgtg                                                                            <  2:4240702/150‑1 (MQ=255)
                                                                         aCCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGtt                                                                         >  1:2423526/1‑150 (MQ=255)
                                                                         aCCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGtt                                                                         <  2:1794906/150‑1 (MQ=255)
                                                                                tGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCttt                                                                  <  1:4592272/150‑1 (MQ=255)
                                                                                   tGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGc                                                               >  1:4859157/1‑150 (MQ=255)
                                                                                      gagCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGaa                                                            >  2:2660939/1‑150 (MQ=255)
                                                                                         cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                         >  2:4327285/1‑150 (MQ=255)
                                                                                         cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                         <  1:2643921/150‑1 (MQ=255)
                                                                                         cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                         <  1:4181638/150‑1 (MQ=255)
                                                                                          gAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATg                                                        <  2:1109587/150‑1 (MQ=255)
                                                                                            gTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATggg                                                      <  1:1414699/150‑1 (MQ=255)
                                                                                               ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                                   >  2:3292/1‑150 (MQ=255)
                                                                                               ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                                   >  2:4354422/1‑150 (MQ=255)
                                                                                               ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                                   <  2:436638/150‑1 (MQ=255)
                                                                                               ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                                   <  2:4804508/150‑1 (MQ=255)
                                                                                                 tATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAg                                                 <  2:874809/150‑1 (MQ=255)
                                                                                                  aTTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGa                                                >  1:3350055/1‑150 (MQ=255)
                                                                                                     tttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAc                                             <  2:1274450/150‑1 (MQ=255)
                                                                                                      ttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcg                                            <  1:1370553/150‑1 (MQ=255)
                                                                                                       tATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcgc                                           >  1:3805206/1‑150 (MQ=255)
                                                                                                        aTCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcgcg                                          >  2:4107976/1‑150 (MQ=255)
                                                                                                          cACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGAc                                        >  2:3949502/1‑150 (MQ=255)
                                                                                                           aCGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACt                                       <  2:1617840/150‑1 (MQ=255)
                                                                                                            cGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACtt                                      <  1:1680290/150‑1 (MQ=255)
                                                                                                              tAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTAt                                    >  2:154738/1‑150 (MQ=255)
                                                                                                              tAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTGATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACGGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTAt                                    <  2:278022/150‑1 (MQ=255)
                                                                                                               aaGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                                   <  1:765155/150‑1 (MQ=255)
                                                                                                                aGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATcc                                  <  1:1980941/150‑1 (MQ=255)
                                                                                                                aGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATcc                                  <  2:3975777/150‑1 (MQ=255)
                                                                                                                     aaTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAt                             >  1:4900871/1‑150 (MQ=255)
                                                                                                                      aTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAtt                            >  1:1207149/1‑150 (MQ=255)
                                                                                                                           cTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCtt                       >  2:5005762/1‑150 (MQ=255)
                                                                                                                                  ggCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATc                >  1:4750439/1‑150 (MQ=255)
                                                                                                                                   gCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCg               <  1:5059007/150‑1 (MQ=255)
                                                                                                                                   gCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCg               <  1:5254769/150‑1 (MQ=255)
                                                                                                                                    cAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGc              >  1:1115212/1‑150 (MQ=255)
                                                                                                                                     aaCAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCa             >  2:3739097/1‑150 (MQ=255)
                                                                                                                                        atttttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAata          >  2:5234123/3‑150 (MQ=255)
                                                                                                                                        aGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAata          <  2:692080/150‑1 (MQ=255)
                                                                                                                                        aGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAata          >  2:2221137/1‑150 (MQ=255)
                                                                                                                                         gTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAatat         >  1:1396892/1‑150 (MQ=255)
                                                                                                                                          ttttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATAtt        <  2:1409551/150‑1 (MQ=255)
                                                                                                                                          ttttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATAtt        <  2:2061272/150‑1 (MQ=255)
                                                                                                                                           tttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTc       <  1:3900834/150‑1 (MQ=255)
                                                                                                                                            ttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCg      <  2:2338953/150‑1 (MQ=255)
                                                                                                                                            ttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTAAGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCg      <  1:1329613/150‑1 (MQ=255)
                                                                                                                                             tACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGt     >  1:2583516/1‑150 (MQ=255)
                                                                                                                                             tACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGt     >  2:2927349/1‑150 (MQ=255)
                                                                                                                                               ccATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCa   <  2:1909695/150‑1 (MQ=255)
                                                                                                                                                cATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCAt  >  2:4676427/1‑150 (MQ=255)
                                                                                                                                                cATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCAt  >  1:4273084/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCAT  >  CP009273/4530996‑4531289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑