Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 32,802 C→A 100% I662I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927332,8020CA98.9% 857.5 / ‑1.7 296I662I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (1/7);  new base A (133/152);  total (134/162)
Fisher's exact test for biased strand distribution p-value = 7.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 296 total aligned reads displayed.

CGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACC  >  CP009273/32655‑32947
                                                                                                                                                   |                                                                                                                                                 
cGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg                                                                                                                                                 <  1:4023743/150‑1 (MQ=255)
cGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg                                                                                                                                                 <  1:1236625/150‑1 (MQ=255)
cGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGGTATAgg                                                                                                                                                 >  2:4360055/1‑150 (MQ=255)
 gACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGc                                                                                                                                                >  1:1189191/1‑150 (MQ=255)
 gACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGc                                                                                                                                                <  1:1282688/150‑1 (MQ=255)
   cagCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCAc                                                                                                                                              >  2:2242802/3‑150 (MQ=255)
    agCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCAcc                                                                                                                                             >  2:1479690/2‑150 (MQ=255)
      ccTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAACCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAg                                                                                                                                           <  2:127266/150‑1 (MQ=255)
      ccTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAg                                                                                                                                           <  2:108780/150‑1 (MQ=255)
      ccTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAg                                                                                                                                           >  2:1471923/1‑150 (MQ=255)
      ccTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAAATGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAg                                                                                                                                           >  1:584032/1‑150 (MQ=255)
       ctctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGc                                                                                                                                          >  2:4912970/1‑150 (MQ=255)
         ctACTTCGAGCCGGTAACTCTGGAAGATGTGTTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGccc                                                                                                                                        <  1:639312/150‑1 (MQ=255)
            cTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGa                                                                                                                                     >  2:704474/1‑150 (MQ=255)
            cTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGa                                                                                                                                     <  2:4244558/150‑1 (MQ=255)
             ttCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGAt                                                                                                                                    <  1:3054485/150‑1 (MQ=255)
              tCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATg                                                                                                                                   <  2:4748504/150‑1 (MQ=255)
                    cGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCg                                                                                                                             <  1:289397/150‑1 (MQ=255)
                      gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGAc                                                                                                                           <  2:3383401/150‑1 (MQ=255)
                         aCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGt                                                                                                                        <  1:4745313/150‑1 (MQ=255)
                            ctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAATGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCACGGATGCTATCGACCGTGCa                                                                                                                     >  1:4394660/1‑150 (MQ=255)
                             tGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCag                                                                                                                    <  1:2432546/150‑1 (MQ=255)
                               gAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaa                                                                                                                  <  2:1494984/150‑1 (MQ=255)
                               gAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaa                                                                                                                  <  1:3403632/150‑1 (MQ=255)
                               gAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaa                                                                                                                  <  1:1684592/150‑1 (MQ=255)
                                   aTGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGAcc                                                                                                              >  2:3738905/1‑150 (MQ=255)
                                   aTGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGAcc                                                                                                              <  2:4963463/150‑1 (MQ=255)
                                      tgCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGGGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGACGCGGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTg                                                                                                           <  1:1469238/150‑1 (MQ=255)
                                      tgCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTg                                                                                                           <  2:1220069/150‑1 (MQ=255)
                                      tgCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTg                                                                                                           <  2:3558214/150‑1 (MQ=255)
                                          ggAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACg                                                                                                       >  1:4109283/1‑150 (MQ=255)
                                           gAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                      >  2:3778740/1‑150 (MQ=255)
                                            aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAAACCGTGAACGCt                                                                                                     >  2:1037839/1‑150 (MQ=255)
                                               tCGTGCGTATCGTGAAGCCGAATGGCGTTATCGTCCTGTCCGGCGGTCAGACCCGGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTTCCGGTTATTGGCACCTGCCGGTATGCTATCGACCGTGCAGAAGACCGTGAACGCTTcc                                                                                                  <  1:106502/150‑1 (MQ=255)
                                               tCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTcc                                                                                                  <  2:4432663/150‑1 (MQ=255)
                                                cGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCa                                                                                                 <  2:388876/150‑1 (MQ=255)
                                                 gTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCTGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAg                                                                                                <  1:5036529/150‑1 (MQ=255)
                                                 gTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAg                                                                                                >  1:2797398/1‑150 (MQ=255)
                                                   gCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAACCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCa                                                                                              <  2:2325356/150‑1 (MQ=255)
                                                   gCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCa                                                                                              <  2:4777976/150‑1 (MQ=255)
                                                    cGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACGGGCGCGCGCGCTGGACGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCAt                                                                                             <  1:4516332/150‑1 (MQ=255)
                                                       aTCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCg                                                                                          >  1:1839501/1‑150 (MQ=255)
                                                           agaCGCCGACTGGCGTTATCGGCCCGTACGGCGGCCCGCCCCCGCTGAAACGGGCGCGCGCGCTGGACGCTGCGGGCGTACCGGTTATCGGCACCTGCCCGGATGCTATCGACCGTGCAGACGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                      <  1:4950260/150‑1 (MQ=255)
                                                             aaGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAg                                                                                    >  1:3594585/1‑150 (MQ=255)
                                                              aGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGc                                                                                   >  2:3639118/1‑150 (MQ=255)
                                                                 cGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTc                                                                                >  2:1141612/1‑150 (MQ=255)
                                                                    aaGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctga                                                                             >  2:2777636/1‑150 (MQ=255)
                                                                     aGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                            >  2:4690847/1‑150 (MQ=255)
                                                                          ttATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactga                                                                       <  1:2974156/150‑1 (MQ=255)
                                                                          ttATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactga                                                                       <  1:2619542/150‑1 (MQ=255)
                                                                           tATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactgaa                                                                      >  2:5079598/1‑150 (MQ=255)
                                                                           tATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactgaa                                                                      <  2:4177588/150‑1 (MQ=255)
                                                                             tCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCtgaaactgaaac                                                                    <  1:104290/150‑1 (MQ=255)
                                                                               gTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaa                                                                  >  2:4041754/1‑150 (MQ=255)
                                                                                 ccAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAAcc                                                                <  2:4855555/150‑1 (MQ=255)
                                                                                  cAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCg                                                               <  2:3183017/150‑1 (MQ=255)
                                                                                    gTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGc                                                             >  1:4228849/1‑150 (MQ=255)
                                                                                     tACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCg                                                            >  2:209450/1‑150 (MQ=255)
                                                                                       cggcggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGaa                                                          >  1:3756031/1‑150 (MQ=255)
                                                                                        ggcgtTCAGACCCCTCTGAATCTGGCGCGCGCGCTGGATGCTGCTGGCTTACCGGTTATAGGCACCAGCCCGTATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAAc                                                         <  2:3204481/150‑1 (MQ=255)
                                                                                         gcggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACg                                                        <  2:2157215/150‑1 (MQ=255)
                                                                                         gcggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACg                                                        <  1:835309/150‑1 (MQ=255)
                                                                                         gcggTAAGACCCCGCTGAAACTGGCGCGCGCGCTGGACGCTGCTGGCGTACCGGTTTTAGGCACCTGCCCGTTTGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACg                                                        <  1:2358841/150‑1 (MQ=255)
                                                                                            gTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCa                                                     >  1:4802702/1‑150 (MQ=255)
                                                                                               aGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGACCGCCACCg                                                  >  2:1599435/1‑150 (MQ=255)
                                                                                               aGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCg                                                  >  1:3637053/1‑150 (MQ=255)
                                                                                               aGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCg                                                  >  2:4371722/1‑150 (MQ=255)
                                                                                                  ccccGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTa                                               >  1:2253934/1‑150 (MQ=255)
                                                                                                  ccccGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTa                                               <  1:4448444/150‑1 (MQ=255)
                                                                                                    ccGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTAcc                                             <  2:585856/150‑1 (MQ=255)
                                                                                                         gAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAt                                        <  2:3061894/150‑1 (MQ=255)
                                                                                                          aatgtGGCGCGGGGGCTGGAAGCTGGTGGCGTACGGGTTTTAGGCACCAGCCCGGATGATATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAtt                                       <  1:2718727/146‑1 (MQ=255)
                                                                                                          aaaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAtt                                       >  1:4082070/1‑150 (MQ=255)
                                                                                                          aaaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAtt                                       <  1:2242802/150‑1 (MQ=255)
                                                                                                           aaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTg                                      <  2:808954/150‑1 (MQ=255)
                                                                                                              tGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGaaa                                   >  2:488272/1‑150 (MQ=255)
                                                                                                                gcgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATg                                 <  2:1930426/150‑1 (MQ=255)
                                                                                                                 cgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATgg                                >  1:5133127/1‑150 (MQ=255)
                                                                                                                  gcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGc                               >  2:4979923/1‑150 (MQ=255)
                                                                                                                   cgcgcgcTGGAAGCTGCTGGCGTACCGTTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGAATGCGTTTGAGCTTCTTAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCg                              <  1:3137801/150‑1 (MQ=255)
                                                                                                                   cgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCg                              >  1:3513474/1‑150 (MQ=255)
                                                                                                                      gcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTa                           <  1:1541224/150‑1 (MQ=255)
                                                                                                                      gcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTa                           <  2:3260979/150‑1 (MQ=255)
                                                                                                                       cgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTag                          >  2:5209485/1‑150 (MQ=255)
                                                                                                                         cTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTagag                        >  1:2088464/1‑150 (MQ=255)
                                                                                                                            gtaggtgctgGCGTACGGGGTATAGGAACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGATTCCAGCATGCGGTTGTGCGTCTGAAACTGAAAAAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                     <  1:198043/145‑1 (MQ=255)
                                                                                                                            gAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                     <  1:87947/150‑1 (MQ=255)
                                                                                                                            gAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                     <  1:3913700/150‑1 (MQ=255)
                                                                                                                             aaGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAgg                    <  1:1395912/150‑1 (MQ=255)
                                                                                                                                ctgctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGa                 <  2:572651/150‑1 (MQ=255)
                                                                                                                                 tgctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGaa                <  1:80600/150‑1 (MQ=255)
                                                                                                                                  gctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGaaa               <  2:4109283/150‑1 (MQ=255)
                                                                                                                                     ggCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAagag            >  2:699362/1‑150 (MQ=255)
                                                                                                                                        gTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAtt         <  1:721068/150‑1 (MQ=255)
                                                                                                                                        gTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAtt         >  2:2616164/1‑150 (MQ=255)
                                                                                                                                         tACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTg        <  1:488379/150‑1 (MQ=255)
                                                                                                                                             ggTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTa    >  1:3828415/1‑150 (MQ=255)
                                                                                                                                             ggTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTa    >  2:1653453/1‑150 (MQ=255)
                                                                                                                                              gTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTAc   >  2:2417262/1‑150 (MQ=255)
                                                                                                                                               ttATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTAcc  <  2:1189191/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACC  >  CP009273/32655‑32947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑