Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,567,278 G→T 100% I166I (ATC→ATA)  mcrC ← 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,567,2780GT98.6% 1172.0 / 4.3 367I166I (ATC→ATA) mcrC5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (199/161);  total (204/163)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 370 total aligned reads displayed.

AGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTT  >  CP009273/4567131‑4567424
                                                                                                                                                   |                                                                                                                                                  
aGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTAt                                                                                                                                                  >  2:2363722/1‑150 (MQ=255)
 gACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTtata                                                                                                                                                 <  1:2923112/150‑1 (MQ=255)
   cGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAAc                                                                                                                                               <  1:1278382/150‑1 (MQ=255)
   cGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAAc                                                                                                                                               >  1:1561791/1‑150 (MQ=255)
    gTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACg                                                                                                                                              >  1:4656507/1‑150 (MQ=255)
      tAATTCACGACGTCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGaa                                                                                                                                            <  2:4145692/150‑1 (MQ=255)
      tAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGaa                                                                                                                                            >  1:4776439/1‑150 (MQ=255)
          tCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTta                                                                                                                                        <  1:1122020/150‑1 (MQ=255)
              gacgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTataat                                                                                                                                    >  1:4990291/1‑150 (MQ=255)
                 ggCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAAc                                                                                                                                 <  2:4609396/150‑1 (MQ=255)
                   cAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAAcgc                                                                                                                               >  1:1841608/1‑150 (MQ=255)
                     aaaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATAGACGATGAATTTGCAGACACTAATAACTAATTTATAATAACGCGt                                                                                                                             >  1:648673/1‑150 (MQ=255)
                      aaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTa                                                                                                                            <  1:5180092/150‑1 (MQ=255)
                      aaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTa                                                                                                                            >  2:1983345/1‑150 (MQ=255)
                       aTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAt                                                                                                                           >  1:3486099/1‑150 (MQ=255)
                       aTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAt                                                                                                                           <  2:1181648/150‑1 (MQ=255)
                         tCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGACACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAttt                                                                                                                         >  2:3998334/1‑150 (MQ=255)
                         tCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAttt                                                                                                                         <  1:2572659/150‑1 (MQ=255)
                          cATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAtttt                                                                                                                        <  2:3114531/150‑1 (MQ=255)
                           aTAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAttttt                                                                                                                       <  2:2876367/150‑1 (MQ=255)
                              aaGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAGATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTcc                                                                                                                    >  1:1696290/1‑150 (MQ=255)
                               aGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCg                                                                                                                   >  2:4278980/1‑150 (MQ=255)
                               aGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCg                                                                                                                   >  2:486824/1‑150 (MQ=255)
                                 aaaCTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGcc                                                                                                                 >  1:1928848/1‑150 (MQ=255)
                                    cTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCAtt                                                                                                              >  1:2672978/1‑150 (MQ=255)
                                    cTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCAtt                                                                                                              >  1:1393201/1‑150 (MQ=255)
                                    cTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCAtt                                                                                                              <  1:1196663/150‑1 (MQ=255)
                                         gATAAAGTAATGACATCTCTTTTTCGTTTCTTACAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCAGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGAt                                                                                                         >  1:5322447/1‑150 (MQ=255)
                                          aTAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATa                                                                                                        >  1:2225693/1‑150 (MQ=255)
                                          aTAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATa                                                                                                        >  1:3164957/1‑150 (MQ=255)
                                           tAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGTTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAg                                                                                                       <  2:4350650/150‑1 (MQ=255)
                                            aaaGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGc                                                                                                      >  1:322553/1‑150 (MQ=255)
                                            aaaGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGc                                                                                                      >  1:4213169/1‑150 (MQ=255)
                                              aGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTg                                                                                                    <  2:2167747/150‑1 (MQ=255)
                                              aGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTg                                                                                                    >  1:901817/1‑150 (MQ=255)
                                               gTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGa                                                                                                   >  1:1100807/1‑150 (MQ=255)
                                                 aaTGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGaaa                                                                                                 >  1:4601221/1‑150 (MQ=255)
                                                    gACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgc                                                                                              >  2:4512403/1‑150 (MQ=255)
                                                    gACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgc                                                                                              >  2:2606513/1‑150 (MQ=255)
                                                     aCATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgct                                                                                             >  2:4658253/1‑150 (MQ=255)
                                                     aCATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgct                                                                                             <  2:3851246/150‑1 (MQ=255)
                                                      cATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgctg                                                                                            >  1:247970/1‑150 (MQ=255)
                                                      cATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgctg                                                                                            >  1:32591/1‑150 (MQ=255)
                                                       aTCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTTACGATGAATTTTCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgctgc                                                                                           >  1:437859/1‑150 (MQ=255)
                                                       aTCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgctgc                                                                                           >  2:2218947/1‑150 (MQ=255)
                                                         ctctTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCgg                                                                                         >  2:884575/1‑150 (MQ=255)
                                                            tttttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGt                                                                                      >  1:3736720/1‑150 (MQ=255)
                                                              tttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATCACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTa                                                                                    >  2:5040733/1‑150 (MQ=255)
                                                               ttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTaa                                                                                   <  1:2510172/150‑1 (MQ=255)
                                                                tCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTaaa                                                                                  <  1:2727669/150‑1 (MQ=255)
                                                                tCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTaaa                                                                                  <  2:1144951/150‑1 (MQ=255)
                                                                  gTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATg                                                                                >  2:2430153/1‑150 (MQ=255)
                                                                   tttctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGa                                                                               >  2:2906161/1‑150 (MQ=255)
                                                                     tctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAag                                                                             <  2:1662516/150‑1 (MQ=255)
                                                                      ctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAaga                                                                            <  1:2305633/150‑1 (MQ=255)
                                                                          cAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGc                                                                        >  2:4192942/1‑150 (MQ=255)
                                                                            aaaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTa                                                                      >  2:1718347/1‑150 (MQ=255)
                                                                             aaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTaa                                                                     >  2:4093252/1‑150 (MQ=255)
                                                                              aTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAAt                                                                    <  2:5265359/150‑1 (MQ=255)
                                                                              aTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAAt                                                                    >  2:2951162/1‑150 (MQ=255)
                                                                               tCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATg                                                                   <  1:725773/150‑1 (MQ=255)
                                                                                cATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGc                                                                  >  2:3602435/1‑150 (MQ=255)
                                                                                  tAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTACATGAAGAGTGCTAATGccc                                                                >  1:490300/1‑150 (MQ=255)
                                                                                  tAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTACTAATGccc                                                                <  2:1527118/150‑1 (MQ=255)
                                                                                   aGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCg                                                               >  1:2151858/1‑150 (MQ=255)
                                                                                         ggTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAAtt                                                         >  1:971264/1‑150 (MQ=255)
                                                                                            aGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTc                                                      >  2:721760/1‑150 (MQ=255)
                                                                                            aGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTc                                                      >  2:3505853/1‑150 (MQ=255)
                                                                                             gtgtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCt                                                     <  2:2750796/150‑1 (MQ=255)
                                                                                                  cTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATaaa                                                <  2:2812742/150‑1 (MQ=255)
                                                                                                        tttGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAAc                                          <  1:4676486/150‑1 (MQ=255)
                                                                                                            aCCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGc                                      >  2:368241/1‑150 (MQ=255)
                                                                                                             ccTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCt                                     <  1:4156352/150‑1 (MQ=255)
                                                                                                               tGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTc                                   <  2:4232794/150‑1 (MQ=255)
                                                                                                               tGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTc                                   <  2:2837767/150‑1 (MQ=255)
                                                                                                                ggAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCa                                  >  2:1466762/1‑150 (MQ=255)
                                                                                                                  aaTAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCAtc                                <  1:447136/150‑1 (MQ=255)
                                                                                                                     aGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATctct                             <  1:3255858/150‑1 (MQ=255)
                                                                                                                     aGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATctct                             >  2:4923396/1‑150 (MQ=255)
                                                                                                                     aGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATctct                             <  2:3341373/150‑1 (MQ=255)
                                                                                                                      gAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTg                            <  2:1032862/150‑1 (MQ=255)
                                                                                                                       aaTTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGa                           >  1:286709/1‑150 (MQ=255)
                                                                                                                        attattGACGATGAATTTTCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGAt                          <  1:1500232/150‑1 (MQ=255)
                                                                                                                           attGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGt                       >  2:576937/1‑150 (MQ=255)
                                                                                                                           attGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGt                       <  1:3532585/150‑1 (MQ=255)
                                                                                                                               aCGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGaa                   <  1:3703601/150‑1 (MQ=255)
                                                                                                                                cGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAAt                  >  1:3583328/1‑150 (MQ=255)
                                                                                                                                 gATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTTCTAATTCCCGGTAATTTTCTATAAAGTTAACGAGCTTCATCTCTGATATTTGAAtt                 >  1:4009991/1‑150 (MQ=255)
                                                                                                                                 gATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAAtt                 <  2:3778125/150‑1 (MQ=255)
                                                                                                                                   tGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTa               >  1:2003234/1‑150 (MQ=255)
                                                                                                                                    gAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTaa              >  2:3160649/1‑150 (MQ=255)
                                                                                                                                     aaTTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAAc             >  1:3877461/1‑150 (MQ=255)
                                                                                                                                      aTTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCAGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACt            >  1:3754871/1‑150 (MQ=255)
                                                                                                                                       tttGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACtt           <  1:205869/150‑1 (MQ=255)
                                                                                                                                           cAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCa       >  1:3961477/1‑150 (MQ=255)
                                                                                                                                            aGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCAt      >  2:2152314/1‑150 (MQ=255)
                                                                                                                                            aGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCAt      <  1:2363722/150‑1 (MQ=255)
                                                                                                                                             gACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTTAGGAGTTAAATGAAGAGTGCTAATGCCCGGTTATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGTATTTAACTTTTCATg     <  1:3054172/150‑1 (MQ=255)
                                                                                                                                             gACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATg     >  2:1262435/1‑150 (MQ=255)
                                                                                                                                                acTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCTTTCAGATAGCTTAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCAGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCtt  <  1:3396086/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTT  >  CP009273/4567131‑4567424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑