Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,584,381 G→T 100% I53I (ATC→ATA)  yjjL ← L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,584,3810GT99.7% 1125.8 / ‑4.4 343I53I (ATC→ATA) yjjLL‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (169/173);  total (169/174)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 348 total aligned reads displayed.

ATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGATATT  >  CP009273/4584234‑4584527
                                                                                                                                                   |                                                                                                                                                  
aTCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                                                                                                                                                  >  1:4675762/1‑150 (MQ=255)
 tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAtt                                                                                                                                                 <  1:2831941/150‑1 (MQ=255)
 tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAtt                                                                                                                                                 <  1:3866514/150‑1 (MQ=255)
   cAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAc                                                                                                                                               >  2:377790/1‑150 (MQ=255)
    aaTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTAAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAcc                                                                                                                                              <  2:1403039/150‑1 (MQ=255)
     aTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAccg                                                                                                                                             <  1:3261684/150‑1 (MQ=255)
         tGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACcgccgc                                                                                                                                         >  1:1430107/1‑150 (MQ=255)
          gggCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCa                                                                                                                                        <  1:669843/150‑1 (MQ=255)
          gggCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCa                                                                                                                                        >  2:3249533/1‑150 (MQ=255)
                 aaGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaata                                                                                                                                 >  1:3453420/1‑150 (MQ=255)
                  aGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaataa                                                                                                                                >  1:5036649/1‑150 (MQ=255)
                   ggcaGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACTAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaataat                                                                                                                               <  1:3780260/147‑1 (MQ=255)
                     caGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAAtaataa                                                                                                                             >  1:211628/2‑150 (MQ=255)
                     caGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAAtaataa                                                                                                                             <  2:3169776/149‑1 (MQ=255)
                      aGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaac                                                                                                                            >  2:3743566/1‑150 (MQ=255)
                      aGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaac                                                                                                                            <  1:4938820/150‑1 (MQ=255)
                       gTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaaca                                                                                                                           >  1:2377334/1‑150 (MQ=255)
                         tGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaacaac                                                                                                                         >  1:5220964/1‑150 (MQ=255)
                         tGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaacaac                                                                                                                         <  2:4665363/150‑1 (MQ=255)
                          gcgcAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACAGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAacaaca                                                                                                                        >  1:3661848/1‑150 (MQ=255)
                            tcAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTACGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTACGGTCGAGATAATATATTACAGCCTCAAAAAATAAAaacaaaaaa                                                                                                                      >  2:5321380/2‑146 (MQ=255)
                               aTCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCg                                                                                                                   >  2:2295177/1‑150 (MQ=255)
                               aTCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCACAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCg                                                                                                                   <  1:843239/150‑1 (MQ=255)
                                  ccGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTCATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATACCAACCTGGCggtg                                                                                                                <  2:3900855/150‑1 (MQ=255)
                                  ccGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCggtg                                                                                                                <  2:2671562/150‑1 (MQ=255)
                                      aaGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGttt                                                                                                            >  2:1758284/1‑150 (MQ=255)
                                      aaGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGttt                                                                                                            <  2:4338724/150‑1 (MQ=255)
                                           aGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATg                                                                                                       <  2:4205963/150‑1 (MQ=255)
                                            gTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGc                                                                                                      <  1:308874/150‑1 (MQ=255)
                                            gTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGc                                                                                                      >  2:4749800/1‑150 (MQ=255)
                                              gAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGt                                                                                                    >  1:4278703/1‑150 (MQ=255)
                                              gAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCACCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGt                                                                                                    >  2:3962290/1‑150 (MQ=255)
                                               aaaaCACGGAGAGCAAAGCTCCGATTTCGGTGGCACTTAATCCCAATTCTTCAAGAATCGTTAAATTTTATAACGACAGCGAACTGCGGTCTAGATAATTTATTACAGCCGCAAAAAATAAAAACAACAAGGCGGTTGTTTGAATGCGta                                                                                                   >  1:2595719/1‑149 (MQ=255)
                                               aaaaCACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGtt                                                                                                   <  1:239906/150‑1 (MQ=255)
                                                      ggAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCg                                                                                            <  2:1365367/150‑1 (MQ=255)
                                                        agagCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATACTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCggg                                                                                          >  2:3582790/1‑150 (MQ=255)
                                                        agagCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCggg                                                                                          <  1:1103566/150‑1 (MQ=255)
                                                           gCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTAc                                                                                       <  2:3030315/150‑1 (MQ=255)
                                                            cAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACt                                                                                      <  1:3314469/150‑1 (MQ=255)
                                                            cAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACt                                                                                      >  2:3809096/1‑150 (MQ=255)
                                                             aaaGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTg                                                                                     >  2:2951947/1‑150 (MQ=255)
                                                                gcgcCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGt                                                                                  <  1:4213749/150‑1 (MQ=255)
                                                                gcgcCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGt                                                                                  >  1:3753006/1‑150 (MQ=255)
                                                                  gcCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTg                                                                                <  2:2626283/150‑1 (MQ=255)
                                                                   ccGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGa                                                                               >  2:3370719/1‑150 (MQ=255)
                                                                    cGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGaa                                                                              >  1:397557/1‑150 (MQ=255)
                                                                    cGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGaa                                                                              >  2:2782677/1‑150 (MQ=255)
                                                                      aTTTCGGTGGCACTTAATCCCATTTATTCTCGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACt                                                                            <  1:3057101/150‑1 (MQ=255)
                                                                      aTTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACt                                                                            <  2:4842896/150‑1 (MQ=255)
                                                                         tCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAAt                                                                         <  1:4995884/150‑1 (MQ=255)
                                                                         tCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAAt                                                                         <  1:3495251/150‑1 (MQ=255)
                                                                           ggTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATcc                                                                       >  2:2199895/1‑150 (MQ=255)
                                                                            gTGGCACTTAATCCCAATTCTTCACGACACGTTAAATTTGCTACCGACAGCGAACAGCGGTCGAGATACAGTATTACCGCCGCAAAAAATAATAACAACATGGCGGTCGGGTGAATGCGTTTCCTTCGGGTCCTGCGTGCAACGAATCCa                                                                      >  2:1457485/1‑150 (MQ=255)
                                                                              ggCACTTAATCCCAATTCTTCACGAATCGTTAAAATTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATc                                                                    <  2:23415/150‑1 (MQ=255)
                                                                                cACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtg                                                                  >  1:4438121/1‑150 (MQ=255)
                                                                                    tAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtggtgg                                                              <  2:2045787/150‑1 (MQ=255)
                                                                                     aaTCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGc                                                             <  2:2099749/150‑1 (MQ=255)
                                                                                      aTCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCa                                                            >  1:4740137/1‑150 (MQ=255)
                                                                                         ccAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACgg                                                         >  2:3495037/1‑150 (MQ=255)
                                                                                          cAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACggg                                                        >  1:2858000/1‑150 (MQ=255)
                                                                                           aaTTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGa                                                       <  1:1807050/150‑1 (MQ=255)
                                                                                            aTTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGac                                                      >  1:1238613/1‑149 (MQ=255)
                                                                                            aTTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGaa                                                      >  2:5246113/1‑150 (MQ=255)
                                                                                                 tcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCt                                                 <  2:3706115/150‑1 (MQ=255)
                                                                                                   aCGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGc                                               >  1:4574962/1‑150 (MQ=255)
                                                                                                     gAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCcg                                             >  1:4367816/1‑150 (MQ=255)
                                                                                                        tCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCcgacg                                          <  2:4524814/150‑1 (MQ=255)
                                                                                                           ttAAATTTGCTAGCGACAGAGAACTGCGTTCGAGATAATTTATTACCTCCTCAAAAAATAATAACAACATGGCGGTTGTTTGAATGCGTTTATTTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAg                                       <  2:340508/150‑1 (MQ=255)
                                                                                                           ttAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAg                                       >  2:4272857/1‑150 (MQ=255)
                                                                                                           ttAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAg                                       <  1:4881621/150‑1 (MQ=255)
                                                                                                            tAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGa                                      >  1:2808979/1‑150 (MQ=255)
                                                                                                             aaaTTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACTTGGCTGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGAt                                     <  2:5011075/150‑1 (MQ=255)
                                                                                                              aaTTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATg                                    >  2:2524422/1‑150 (MQ=255)
                                                                                                               aTTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATgg                                   >  1:4349658/1‑150 (MQ=255)
                                                                                                                 ttGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAg                                 <  2:13116/150‑1 (MQ=255)
                                                                                                                     tACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTaaa                             >  1:4051857/1‑150 (MQ=255)
                                                                                                                      aCCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACACCATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAAt                            <  2:5174138/150‑1 (MQ=255)
                                                                                                                      aCCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAAt                            <  2:495216/150‑1 (MQ=255)
                                                                                                                        cGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGGGTAAATga                          >  2:5026281/1‑150 (MQ=255)
                                                                                                                        cGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATga                          >  1:242399/1‑150 (MQ=255)
                                                                                                                          aCAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaag                        <  2:4628563/150‑1 (MQ=255)
                                                                                                                           cAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaaga                       >  2:2900195/1‑150 (MQ=255)
                                                                                                                            aGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaagaa                      >  1:132292/1‑150 (MQ=255)
                                                                                                                              cGAACTGCGGTAGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAAcg                    <  1:2919049/150‑1 (MQ=255)
                                                                                                                               gAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAAcgc                   >  2:1902717/1‑150 (MQ=255)
                                                                                                                                   tGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGAt               <  1:1758288/150‑1 (MQ=255)
                                                                                                                                    gCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACCTGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATc              <  2:4068572/150‑1 (MQ=255)
                                                                                                                                    gCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATc              <  2:3082372/150‑1 (MQ=255)
                                                                                                                                     cGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCg             <  1:337865/150‑1 (MQ=255)
                                                                                                                                        tCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATg          <  1:1551768/150‑1 (MQ=255)
                                                                                                                                           agaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTg       >  1:1896166/1‑150 (MQ=255)
                                                                                                                                            gaTAATTTATTACCGCCGCAAAAAATAATAACAACCTGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGa      <  2:3821800/150‑1 (MQ=255)
                                                                                                                                            gaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGa      >  1:2536010/1‑150 (MQ=255)
                                                                                                                                             aTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGat     >  2:1258044/1‑150 (MQ=255)
                                                                                                                                               aaTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGatat   >  2:1682936/1‑150 (MQ=255)
                                                                                                                                               aaTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGatat   >  1:1059347/1‑150 (MQ=255)
                                                                                                                                               aaTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGatat   <  2:1130168/150‑1 (MQ=255)
                                                                                                                                               aaTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGatat   >  1:2651394/1‑150 (MQ=255)
                                                                                                                                               aaTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGatat   <  1:1685794/150‑1 (MQ=255)
                                                                                                                                               aaTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGatat   >  2:945303/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGATATT  >  CP009273/4584234‑4584527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑