Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,596,243 T→A 100% intergenic (‑25/+243) leuQ ← / ← rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,596,2430TA99.3% 985.9 / ‑2.9 299intergenic (‑25/+243)leuQ/rsmCtRNA‑Leu/16S rRNA m(2)G1207 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (183/114);  total (185/114)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 299 total aligned reads displayed.

ACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCT  >  CP009273/4596096‑4596389
                                                                                                                                                   |                                                                                                                                                  
aCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGc                                                                                                                                                  >  1:4218831/1‑150 (MQ=255)
  cTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTa                                                                                                                                                >  1:311469/1‑150 (MQ=255)
    tCGCCCCGTGCGAGCACTCTCCCCGTGGTTTTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTAcc                                                                                                                                              <  1:671348/150‑1 (MQ=18)
              cGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGgcgc                                                                                                                                    >  2:4874777/1‑150 (MQ=255)
              cGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGgcgc                                                                                                                                    >  2:2989339/1‑150 (MQ=255)
                aGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCAt                                                                                                                                  >  1:2291103/1‑150 (MQ=255)
                  aaaTATCAACTAGTTTTTTGGTGCGAGGGGGGGTACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTc                                                                                                                                <  1:1012443/149‑1 (MQ=21)
                   aaTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCt                                                                                                                               >  1:290932/1‑150 (MQ=255)
                   aaTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCt                                                                                                                               >  1:3566466/1‑150 (MQ=255)
                     tatCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAg                                                                                                                             >  2:1235451/1‑150 (MQ=255)
                      atCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGt                                                                                                                            <  1:5294913/150‑1 (MQ=255)
                       tctccGTGGCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTg                                                                                                                           <  1:1622370/146‑1 (MQ=255)
                             tgttttttGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTc                                                                                                                     <  1:1394811/147‑1 (MQ=255)
                             tgttttttGGGGCGTGGGGGGGTACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTc                                                                                                                     <  1:4900766/147‑1 (MQ=21)
                             tGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCAACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTc                                                                                                                     <  2:2851825/150‑1 (MQ=255)
                              gttttttGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCa                                                                                                                    <  1:2959169/148‑1 (MQ=255)
                              ggTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCa                                                                                                                    >  2:1444254/1‑150 (MQ=255)
                               gTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAg                                                                                                                   <  2:626261/150‑1 (MQ=255)
                                    tGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCtttt                                                                                                              >  1:901048/1‑150 (MQ=255)
                                     ggTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTc                                                                                                             <  1:999118/150‑1 (MQ=255)
                                     ggTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTc                                                                                                             >  2:1362399/1‑150 (MQ=255)
                                                ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACGTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTa                                                                                                  >  1:3011118/1‑150 (MQ=255)
                                                ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTa                                                                                                  >  2:2526189/1‑150 (MQ=255)
                                                    aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTa                                                                                              <  1:5116287/150‑1 (MQ=255)
                                                     cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTAt                                                                                             <  1:2180704/150‑1 (MQ=255)
                                                      ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATc                                                                                            >  1:3401516/1‑150 (MQ=255)
                                                       tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCg                                                                                           >  1:2507275/1‑150 (MQ=255)
                                                       tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCg                                                                                           >  1:603713/1‑150 (MQ=255)
                                                          aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACa                                                                                        >  2:3489732/1‑150 (MQ=255)
                                                             cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACagag                                                                                     >  1:1253878/1‑150 (MQ=255)
                                                              ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAgg                                                                                    >  1:2588525/1‑150 (MQ=255)
                                                              ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAgg                                                                                    >  2:3260580/1‑150 (MQ=255)
                                                              ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAgg                                                                                    <  2:4039032/150‑1 (MQ=255)
                                                               cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGgt                                                                                   <  1:2348419/150‑1 (MQ=255)
                                                                    ccGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAAt                                                                              <  1:2439362/150‑1 (MQ=255)
                                                                     cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAAtt                                                                             <  1:1771742/150‑1 (MQ=255)
                                                                     cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAAtt                                                                             <  1:5194924/150‑1 (MQ=255)
                                                                        aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCt                                                                          >  2:4415129/1‑150 (MQ=255)
                                                                         aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTg                                                                         >  1:4335826/1‑150 (MQ=255)
                                                                            acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGaa                                                                      <  1:1917577/150‑1 (MQ=255)
                                                                              acTACCCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCGACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGaaaa                                                                    <  2:4658635/150‑1 (MQ=255)
                                                                                tAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAAt                                                                  <  1:1109050/150‑1 (MQ=255)
                                                                                tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTc                                                                                                                     >  2:2441218/1‑99 (MQ=255)
                                                                                tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAAt                                                                  <  1:3780396/150‑1 (MQ=255)
                                                                                 aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATg                                                                 >  1:4261960/1‑150 (MQ=255)
                                                                                    acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTcc                                                              <  1:3861345/150‑1 (MQ=255)
                                                                                     ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCa                                                             <  2:3333822/150‑1 (MQ=255)
                                                                                      cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCAt                                                            >  2:4363984/1‑150 (MQ=255)
                                                                                      cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCAt                                                            >  1:1363226/1‑150 (MQ=255)
                                                                                       tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGACAAATGTCCATc                                                           >  1:2170409/1‑150 (MQ=255)
                                                                                        gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCa                                                          <  1:1447053/150‑1 (MQ=255)
                                                                                           gCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCAATCATGa                                                       >  1:1691399/1‑150 (MQ=255)
                                                                                             tAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGaaa                                                     >  1:4934083/1‑150 (MQ=255)
                                                                                                cgcgTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCCTCAGGAAACTa                                                  >  1:3436777/1‑150 (MQ=255)
                                                                                                 gcgTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAg                                                 >  2:3772129/1‑150 (MQ=255)
                                                                                                 gcgTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAg                                                 >  2:4972848/1‑150 (MQ=255)
                                                                                                   gTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCg                                               >  1:4288684/1‑150 (MQ=255)
                                                                                                       aCCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCa                                           <  2:311469/150‑1 (MQ=255)
                                                                                                        ccAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAATTGTCCATCAGGAAACTAGCGTGCAg                                          >  2:4958335/1‑150 (MQ=255)
                                                                                                        ccAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAg                                          <  2:5297140/150‑1 (MQ=255)
                                                                                                          aaTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGt                                        >  2:368087/1‑150 (MQ=255)
                                                                                                          aaTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGt                                        >  2:689173/1‑150 (MQ=255)
                                                                                                           aTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGtt                                       <  1:1165683/150‑1 (MQ=255)
                                                                                                           aTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGtt                                       <  1:1991921/150‑1 (MQ=255)
                                                                                                           aTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGtt                                       >  2:4247273/1‑150 (MQ=255)
                                                                                                            ttCCGCCACCTTCGCATACCATCAATTATTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGttt                                      <  2:2308946/150‑1 (MQ=255)
                                                                                                               cGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGt                                   >  2:1554128/1‑150 (MQ=255)
                                                                                                                 ccACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTat                                 <  2:2410794/150‑1 (MQ=255)
                                                                                                                  cACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTatg                                >  1:3284352/1‑150 (MQ=255)
                                                                                                                    ccTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTatgca                              <  2:1203519/150‑1 (MQ=255)
                                                                                                                    ccTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTatgca                              >  1:3999133/1‑150 (MQ=255)
                                                                                                                     cTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTGTGGTatgcat                             >  1:3259931/1‑150 (MQ=255)
                                                                                                                       tCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTatgcatgc                           <  2:1024569/150‑1 (MQ=255)
                                                                                                                         gCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCgg                         >  1:742391/1‑150 (MQ=255)
                                                                                                                         gCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCgg                         >  1:4661246/1‑150 (MQ=255)
                                                                                                                          cATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCggg                        >  2:2239792/1‑150 (MQ=255)
                                                                                                                           aTACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCgggg                       >  2:44862/1‑150 (MQ=255)
                                                                                                                           aTACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTGTGGTATGCATGCgggg                       >  2:1862433/1‑150 (MQ=255)
                                                                                                                            tACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCggggg                      >  1:4831029/1‑150 (MQ=255)
                                                                                                                             aCCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGc                     >  2:3307973/1‑150 (MQ=255)
                                                                                                                              ccATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGACCATAAGGAAACTAGCGTGCATGTTTGGTATGCATGCGGGGGca                    >  1:3900220/1‑150 (MQ=255)
                                                                                                                               cATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcag                   >  2:2581097/1‑150 (MQ=255)
                                                                                                                               cATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTAGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCGGGTTTGGTATGCATGCGGGGGcag                   >  1:3643974/1‑150 (MQ=255)
                                                                                                                                  cAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatg                <  1:1817988/150‑1 (MQ=255)
                                                                                                                                    aTTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgcc              >  2:749223/1‑150 (MQ=255)
                                                                                                                                     ttCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgcca             <  1:2443945/150‑1 (MQ=255)
                                                                                                                                     ttCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgcca             <  1:3157033/150‑1 (MQ=255)
                                                                                                                                     ttCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgcca             <  2:4651035/150‑1 (MQ=255)
                                                                                                                                      tattAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccag            <  1:4140980/148‑1 (MQ=255)
                                                                                                                                        ttAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTGTGGTATGCATGCGGGGGcagatgccagat          >  2:2807949/1‑150 (MQ=255)
                                                                                                                                         tAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccagatg         <  1:2726774/150‑1 (MQ=255)
                                                                                                                                          aaaaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccagatgc        <  2:917293/150‑1 (MQ=255)
                                                                                                                                          aaaaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccagatgc        <  2:1712108/150‑1 (MQ=255)
                                                                                                                                           aaaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCg       <  2:4744413/150‑1 (MQ=255)
                                                                                                                                            aaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAAGTATCGACCGAGGTGTAATTGCTGGACACATGTCCATCAGGAAACTAGCGTGCAGGGTTGGTATGCATGCGGGGGCTGATGCCAGAGGCGa      >  2:578776/1‑150 (MQ=255)
                                                                                                                                            aaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTATCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGa      >  2:2949575/1‑150 (MQ=255)
                                                                                                                                             aaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGAc     >  2:5030169/1‑150 (MQ=255)
                                                                                                                                              aGAATAGCTACCACGGAGGCTCATTCTAGTGTTTTTCAGCTTTTAGTCCATAGTTACTTATCGACAGAGGTGTATTTGCTGGTAATTTGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACg    <  1:63575/150‑1 (MQ=255)
                                                                                                                                              aGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACg    >  2:3981810/1‑150 (MQ=255)
                                                                                                                                                aaTAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGAGGCGACGCt  >  2:3326261/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCT  >  CP009273/4596096‑4596389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑