Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 362,629 A→T 100% Y9N (TAC→AAC)  mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273362,6290AT98.4% 978.5 / 2.9 302Y9N (TAC→AAC) mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base T (135/162);  total (139/163)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 314 total aligned reads displayed.

CTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTT  >  CP009273/362482‑362778
                                                                                                                                                   |                                                                                                                                                     
ctTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGgttttgtttt                                                                                                                                                     <  1:1463163/150‑1 (MQ=255)
ctTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGgttttgtttt                                                                                                                                                     <  2:2977170/150‑1 (MQ=255)
ctTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGgttttgtttt                                                                                                                                                     <  1:2423331/150‑1 (MQ=255)
ctTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGgttttgtttt                                                                                                                                                     >  2:4177842/1‑150 (MQ=255)
 ttCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTc                                                                                                                                                    >  2:927877/1‑150 (MQ=255)
  tCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTcc                                                                                                                                                   <  1:1046054/150‑1 (MQ=255)
      tcAGCTTCTCCATCAGTCGCCGAACAGTGGTGCGATTCAGGCCGCTGAGTTACGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCTGGTTAAGCCGCTCACGGTTTTGTTTTCCGTCt                                                                                                                                               <  2:362410/149‑1 (MQ=255)
      gctgCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGAACCGCAATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCt                                                                                                                                               <  1:4271745/147‑1 (MQ=255)
      gCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCt                                                                                                                                               <  2:1250844/150‑1 (MQ=255)
       cAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTg                                                                                                                                              <  2:3756651/150‑1 (MQ=255)
          cGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTc                                                                                                                                           >  2:2032414/1‑150 (MQ=255)
           gTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCa                                                                                                                                          >  1:2920629/1‑150 (MQ=255)
           gTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTGTGTTTTCCGTCTGCTCa                                                                                                                                          >  2:4107233/1‑150 (MQ=255)
            tctcCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCAt                                                                                                                                         <  2:2119279/150‑1 (MQ=255)
            tctcCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCAt                                                                                                                                         >  1:1575020/1‑150 (MQ=255)
            tctcCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCAt                                                                                                                                         <  2:5064259/150‑1 (MQ=255)
             ctcCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCAtt                                                                                                                                        <  1:4139774/150‑1 (MQ=255)
                  gcagTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCt                                                                                                                                   <  1:5166796/150‑1 (MQ=255)
                   cagTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTg                                                                                                                                  <  2:4613900/150‑1 (MQ=255)
                   cagTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTg                                                                                                                                  <  1:3734138/150‑1 (MQ=255)
                     gTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCa                                                                                                                                <  2:3276409/150‑1 (MQ=255)
                     gTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCa                                                                                                                                >  1:4904793/1‑150 (MQ=255)
                          cgcACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTa                                                                                                                           >  1:5219537/1‑150 (MQ=255)
                            cacaGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAAt                                                                                                                         >  2:2508159/1‑150 (MQ=255)
                             acaGTGGTGCGGTGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAAtt                                                                                                                        >  1:2767838/1‑150 (MQ=255)
                             acaGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCAtatttatt                                                                                                                        <  1:3920045/150‑1 (MQ=255)
                              caGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTg                                                                                                                       <  1:4685615/150‑1 (MQ=255)
                              caGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTg                                                                                                                       <  1:4180640/150‑1 (MQ=255)
                               aGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGa                                                                                                                      <  1:994578/150‑1 (MQ=255)
                               aGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGa                                                                                                                      >  2:3527218/1‑150 (MQ=255)
                                 tggtgCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACa                                                                                                                    <  2:1196419/150‑1 (MQ=255)
                                    tgCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACAttt                                                                                                                 <  1:3278449/150‑1 (MQ=255)
                                    tgCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACAttt                                                                                                                 >  1:4977096/1‑150 (MQ=255)
                                     gCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTc                                                                                                                <  1:454936/150‑1 (MQ=255)
                                        aTGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCtat                                                                                                             <  2:4032426/150‑1 (MQ=255)
                                         tGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCtata                                                                                                            <  1:1483972/150‑1 (MQ=255)
                                            aGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGtt                                                                                                         >  1:1320386/1‑150 (MQ=255)
                                             ggCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTa                                                                                                        <  1:3590497/150‑1 (MQ=255)
                                                    gAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAaacaac                                                                                                 >  1:2539581/1‑150 (MQ=255)
                                                    gAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAaacaac                                                                                                 <  2:810198/150‑1 (MQ=255)
                                                        tCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACgtgg                                                                                             <  2:4442069/150‑1 (MQ=255)
                                                         ccGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACgtggt                                                                                            <  1:4391831/150‑1 (MQ=255)
                                                            ccAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCa                                                                                         <  2:4319017/150‑1 (MQ=255)
                                                                    ccGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGc                                                                                 >  1:5054056/1‑150 (MQ=255)
                                                                     cGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGca                                                                                >  2:4826769/1‑150 (MQ=255)
                                                                        cGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACAt                                                                             <  1:2753094/150‑1 (MQ=255)
                                                                         gCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACAtt                                                                            <  2:3945142/150‑1 (MQ=255)
                                                                          cTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTc                                                                           <  2:5018729/150‑1 (MQ=255)
                                                                                 cGCCATCAATTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATg                                                                    <  1:4589712/150‑1 (MQ=255)
                                                                                 cGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATg                                                                    <  2:475874/150‑1 (MQ=255)
                                                                                  gCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGt                                                                   >  1:5197094/1‑150 (MQ=255)
                                                                                   ccATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGtt                                                                  <  1:313052/150‑1 (MQ=255)
                                                                                     aTCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGtttt                                                                <  2:896655/150‑1 (MQ=255)
                                                                                      tCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTg                                                               >  1:4113620/1‑150 (MQ=255)
                                                                                        aaGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAt                                                             <  2:4861743/150‑1 (MQ=255)
                                                                                        aaGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAt                                                             <  2:1340409/150‑1 (MQ=255)
                                                                                        aaGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAt                                                             >  2:169952/1‑150 (MQ=255)
                                                                                         aGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAtt                                                            <  2:2009486/150‑1 (MQ=255)
                                                                                         aGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGAtt                                                            <  1:805455/150‑1 (MQ=255)
                                                                                             tatttaACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAg                                                        >  1:2602841/1‑150 (MQ=255)
                                                                                                ttaACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCg                                                     >  2:5216038/1‑150 (MQ=255)
                                                                                                 taACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGa                                                    >  2:1418099/1‑150 (MQ=255)
                                                                                                  aaCATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGaa                                                   >  2:4539308/1‑150 (MQ=255)
                                                                                                  aaCATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGaa                                                   >  1:685382/1‑150 (MQ=255)
                                                                                                    cATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGaaaa                                                 <  2:4649134/150‑1 (MQ=255)
                                                                                                     atatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAac                                                <  1:3376266/150‑1 (MQ=255)
                                                                                                     atatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAac                                                <  2:1060936/150‑1 (MQ=255)
                                                                                                     atatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAac                                                <  2:3075279/150‑1 (MQ=255)
                                                                                                      tatTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAaca                                               >  1:2468889/1‑150 (MQ=255)
                                                                                                         ttAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACAccc                                            <  2:24358/150‑1 (MQ=255)
                                                                                                         ttAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACAccc                                            >  2:4554693/1‑150 (MQ=255)
                                                                                                         ttAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACAccc                                            >  2:4835916/1‑150 (MQ=255)
                                                                                                          taataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCt                                           <  1:2841726/150‑1 (MQ=255)
                                                                                                          taataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTATGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCt                                           <  2:4079744/150‑1 (MQ=255)
                                                                                                           aataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCtt                                          >  1:624852/1‑150 (MQ=255)
                                                                                                           aataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCtt                                          <  2:4904797/150‑1 (MQ=255)
                                                                                                           aataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCtt                                          <  2:736799/150‑1 (MQ=255)
                                                                                                           aataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCtt                                          >  1:670999/1‑150 (MQ=255)
                                                                                                            ataaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACCTTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTc                                         >  2:580057/1‑150 (MQ=255)
                                                                                                             taaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTcc                                        >  2:3388747/1‑150 (MQ=255)
                                                                                                             taaCATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTcc                                        <  1:4746862/150‑1 (MQ=255)
                                                                                                                 aTTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCtata                                    >  2:677847/1‑150 (MQ=255)
                                                                                                                 aTTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCtata                                    <  1:4775788/150‑1 (MQ=255)
                                                                                                                   tAGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACt                                  <  2:1575020/150‑1 (MQ=255)
                                                                                                                    aGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTg                                 >  1:1192767/1‑150 (MQ=255)
                                                                                                                    aGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTg                                 <  2:2285701/150‑1 (MQ=255)
                                                                                                                    aGACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTg                                 <  1:4043785/150‑1 (MQ=255)
                                                                                                                     gACCGCGGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGTAAACACCCTTCCTATACTGa                                <  1:2620102/150‑1 (MQ=255)
                                                                                                                          cgGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGca                           >  1:1826458/1‑150 (MQ=255)
                                                                                                                          cgGGTTAAGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGca                           <  1:1981323/150‑1 (MQ=255)
                                                                                                                                aaGCCGCGCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAACACACCCTTCCTATACTGAGCGCACAATaa                     >  2:274660/1‑150 (MQ=255)
                                                                                                                                    cgcgCACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATaaaaaa                 <  2:381953/150‑1 (MQ=255)
                                                                                                                                     gcgcACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAAt                <  1:1626060/150‑1 (MQ=255)
                                                                                                                                        cACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCAt             <  1:2032414/150‑1 (MQ=255)
                                                                                                                                        cACGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCAt             <  2:1758180/150‑1 (MQ=255)
                                                                                                                                          cGGTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCAttt           <  2:3706329/150‑1 (MQ=255)
                                                                                                                                           ggTTTTGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTa          <  1:934022/150‑1 (MQ=255)
                                                                                                                                              tttGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACAt       <  2:2213918/150‑1 (MQ=255)
                                                                                                                                               ttGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATg      <  2:1458037/150‑1 (MQ=255)
                                                                                                                                                tGTTTTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGt     >  1:2273111/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCTGGCACCGCCATCAAGTTTATTTAACATATTTAATAACATTAGACCGCGGGTTAAGCCGCGCACGGTTTTGTATTCCGTCTGCTCATTGTTCTGCATATTAATTGACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTT  >  CP009273/362482‑362778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑