Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 390,551 T→G 100% L197R (CTG→CGG)  yaiV → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273390,5510TG99.7% 1221.0 / ‑5.5 308L197R (CTG→CGG) yaiVputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (155/151);  total (156/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 309 total aligned reads displayed.

CAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACA  >  CP009273/390404‑390697
                                                                                                                                                   |                                                                                                                                                  
cAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACggg                                                                                                                                                  <  1:1931840/150‑1 (MQ=255)
 aaTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGt                                                                                                                                                 >  1:3603643/1‑150 (MQ=255)
 aaTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGt                                                                                                                                                 <  1:4146116/150‑1 (MQ=255)
 aaTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGt                                                                                                                                                 >  2:3738169/1‑150 (MQ=255)
  aTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTc                                                                                                                                                <  2:3140317/150‑1 (MQ=255)
  aTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTc                                                                                                                                                <  2:5302580/150‑1 (MQ=255)
    gATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGc                                                                                                                                              >  1:1362495/1‑150 (MQ=255)
     aTTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCt                                                                                                                                             >  2:3633694/1‑150 (MQ=255)
      ttGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTa                                                                                                                                            <  2:1210385/150‑1 (MQ=255)
         aCTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCa                                                                                                                                         <  1:3838660/150‑1 (MQ=255)
            ggAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAcc                                                                                                                                      <  2:3816716/150‑1 (MQ=255)
             gAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCg                                                                                                                                     >  1:3384549/1‑150 (MQ=255)
              aaTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGt                                                                                                                                    >  2:3963450/1‑150 (MQ=255)
               aTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGtt                                                                                                                                   >  1:3860225/1‑150 (MQ=255)
               aTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGtt                                                                                                                                   <  2:5126789/150‑1 (MQ=255)
               aTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGtt                                                                                                                                   >  1:2217622/1‑150 (MQ=255)
                tGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGttt                                                                                                                                  <  1:2782906/150‑1 (MQ=255)
                 gaagaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTg                                                                                                                                 >  2:4929116/1‑150 (MQ=255)
                  aagaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGc                                                                                                                                >  2:4435997/1‑150 (MQ=255)
                     atttGCGATCACGTATTGGTGTTATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCTTAGAAGAATTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCAtt                                                                                                                             <  1:5223593/148‑1 (MQ=255)
                           gATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGt                                                                                                                       >  2:102060/1‑150 (MQ=255)
                           gATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGt                                                                                                                       <  1:3218343/150‑1 (MQ=255)
                           gATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGt                                                                                                                       >  2:5072870/1‑150 (MQ=255)
                           gATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGt                                                                                                                       >  2:24114/1‑150 (MQ=255)
                             tCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAt                                                                                                                     >  2:481820/1‑150 (MQ=255)
                              cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAtt                                                                                                                    <  1:932095/150‑1 (MQ=255)
                              cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAtt                                                                                                                    <  1:80108/150‑1 (MQ=255)
                              cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAtt                                                                                                                    <  1:2459086/150‑1 (MQ=255)
                                       gtgtTATTAATTATATCCATCAACGTACACGAATATCGCGTTCTGTCGTCGCAGAATTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATTAATAAAGGCAAACGGGTCGATATCAACCGTTTGCCTTCAGAGTATTAATCAGGAc                                                                                                           <  1:5093581/150‑1 (MQ=255)
                                       gtgtGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAc                                                                                                           <  2:4185275/150‑1 (MQ=255)
                                        tgtgATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcg                                                                                                          >  1:4778948/1‑150 (MQ=255)
                                        tgtgATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcg                                                                                                          <  2:2332693/150‑1 (MQ=255)
                                         gtgATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcgc                                                                                                         >  2:1649299/1‑150 (MQ=255)
                                         gtgATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCGTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTCTTAATCAGGAcgc                                                                                                         >  2:2943948/1‑150 (MQ=255)
                                          tgatgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcgcg                                                                                                        <  2:1525792/150‑1 (MQ=255)
                                            atgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCggg                                                                                                      >  1:4853706/1‑150 (MQ=255)
                                            atgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCggg                                                                                                      >  2:3413084/1‑150 (MQ=255)
                                              gaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGa                                                                                                    <  2:4092314/150‑1 (MQ=255)
                                              gaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGa                                                                                                    >  1:5301944/1‑150 (MQ=255)
                                               aaTTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGAt                                                                                                   <  1:4636807/150‑1 (MQ=255)
                                                 ttATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATCTCGAACTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCATCCGAGTCTGAATCAGGACGCGGGGATaa                                                                                                 >  1:614710/1‑150 (MQ=255)
                                                 ttATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATaa                                                                                                 >  2:505735/1‑150 (MQ=255)
                                                   atatCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAAcc                                                                                               >  1:1842792/1‑150 (MQ=255)
                                                        cATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACgg                                                                                          <  1:1043803/150‑1 (MQ=255)
                                                         aTCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGt                                                                                         <  1:1294938/150‑1 (MQ=255)
                                                           cAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGttt                                                                                       <  2:1091003/150‑1 (MQ=255)
                                                           cAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGttt                                                                                       <  1:886423/150‑1 (MQ=255)
                                                           cAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGttt                                                                                       <  1:1657455/150‑1 (MQ=255)
                                                           cAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGttt                                                                                       >  2:1843613/1‑150 (MQ=255)
                                                               gTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAGTAATCAGGACGCGGGGATAACCAACGGTTTATTc                                                                                   >  2:2263696/1‑150 (MQ=255)
                                                                 acacGCATTTCGCGTTCTGTCGTCGCAGTAGTTCTCGCTGCTTTGCGTAAAGGCGGATTTTTCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTccc                                                                                 <  1:3713906/150‑1 (MQ=255)
                                                                 acacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATCTCGAAATGAATACAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTccc                                                                                 <  2:4272313/150‑1 (MQ=255)
                                                                  cacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGATATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCg                                                                                <  1:156309/150‑1 (MQ=255)
                                                                     gCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCtt                                                                             >  1:1115316/1‑150 (MQ=255)
                                                                      cATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTa                                                                            <  2:4724470/150‑1 (MQ=255)
                                                                       atatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAg                                                                           <  2:1311808/150‑1 (MQ=255)
                                                                           cgcgTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCg                                                                       >  2:2681120/1‑150 (MQ=255)
                                                                             cgTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGt                                                                     >  1:2238807/1‑150 (MQ=255)
                                                                              gTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTc                                                                    >  2:4891804/1‑150 (MQ=255)
                                                                               ttCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCa                                                                   >  2:1224841/1‑150 (MQ=255)
                                                                                 cTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCTGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCAcc                                                                 <  2:3365163/150‑1 (MQ=255)
                                                                                  tGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCa                                                                >  1:4059927/1‑150 (MQ=255)
                                                                                    tcgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAgg                                                              <  2:4602921/150‑1 (MQ=255)
                                                                                          cAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTg                                                        <  2:2063629/150‑1 (MQ=255)
                                                                                          cAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTg                                                        <  1:3457164/150‑1 (MQ=255)
                                                                                           aGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCCTTGa                                                       >  1:2968170/1‑150 (MQ=255)
                                                                                              aGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGc                                                    >  2:765001/1‑150 (MQ=255)
                                                                                                 tctcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCAt                                                 >  2:2863746/1‑150 (MQ=255)
                                                                                                  ctcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATc                                                <  2:144550/150‑1 (MQ=255)
                                                                                                  ctcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATc                                                <  1:4010243/150‑1 (MQ=255)
                                                                                                   tcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATCAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCg                                               <  2:3043748/150‑1 (MQ=255)
                                                                                                     gctgctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCt                                             <  2:2569028/150‑1 (MQ=255)
                                                                                                     gctgctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCt                                             <  1:1912564/150‑1 (MQ=255)
                                                                                                        gctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCat                                          >  2:5207374/1‑150 (MQ=255)
                                                                                                        gctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGGATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCat                                          >  2:3224909/1‑150 (MQ=255)
                                                                                                         ctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCata                                         <  1:4394215/150‑1 (MQ=255)
                                                                                                          tttGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCatat                                        <  2:3511146/150‑1 (MQ=255)
                                                                                                            tGCGTAAAGGCGGCTATATCGAAATGAATAAAGTCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGATTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATAttt                                      <  1:352923/150‑1 (MQ=255)
                                                                                                            tGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATAttt                                      <  2:2741067/150‑1 (MQ=255)
                                                                                                            tGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATAttt                                      >  1:4895883/1‑150 (MQ=255)
                                                                                                               gTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTa                                   >  1:1716126/1‑150 (MQ=255)
                                                                                                                tAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTaa                                  >  1:3790148/1‑150 (MQ=255)
                                                                                                                   aGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCATCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAgc                               >  2:4708730/1‑150 (MQ=255)
                                                                                                                       ggcTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGt                           >  1:4237336/1‑150 (MQ=255)
                                                                                                                         cTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCa                         >  1:2121763/1‑150 (MQ=255)
                                                                                                                            tatCGAAATGAATAAAGGCAAACGGGTCGATATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                      <  2:1700408/150‑1 (MQ=255)
                                                                                                                                 aaaTGAATAAAGGCAAACGGGTCGCTATCAACCGTGTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGaa                 <  2:3603643/150‑1 (MQ=255)
                                                                                                                                   aTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACg               >  2:4539123/1‑150 (MQ=255)
                                                                                                                                   aTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACg               <  1:5040210/150‑1 (MQ=255)
                                                                                                                                   aTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACg               <  1:3344648/150‑1 (MQ=255)
                                                                                                                                     gAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAg             >  2:2080650/1‑150 (MQ=255)
                                                                                                                                     gAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAg             >  2:3304141/1‑150 (MQ=255)
                                                                                                                                     gAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTAGTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAg             <  2:3013552/150‑1 (MQ=255)
                                                                                                                                      aaTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGt            <  1:2146686/150‑1 (MQ=255)
                                                                                                                                      aaTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGGATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCCTTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGt            >  2:2187048/1‑150 (MQ=255)
                                                                                                                                       aTAAAGGCAAACGGGTCGATATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTg           <  2:1943283/150‑1 (MQ=255)
                                                                                                                                              cAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGAccacca    >  2:2613732/1‑150 (MQ=255)
                                                                                                                                               aaaCGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACcaccac   >  1:2646982/1‑150 (MQ=255)
                                                                                                                                               aaaCGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACcaccac   >  2:923028/1‑150 (MQ=255)
                                                                                                                                                aaCGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACcaca  <  2:833500/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACA  >  CP009273/390404‑390697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑