Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 434,663 A→C 100% I324S (ATC→AGC)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273434,6630AC100.0% 1451.6 / ‑6.7 365I324S (ATC→AGC) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (185/179);  total (185/180)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 366 total aligned reads displayed.

CCGCGTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTGC  >  CP009273/434514‑434809
                                                                                                                                                     |                                                                                                                                                  
cagcgTTTTGCTCTGCAATTTCCACGTCGAATTATCGATCCTGGAATTTACGTGAAAACTCGACCATGCCGGAAACTTCACGCATCGCCGTAGTAATCTCAATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGa                                                                                                                                                    >  2:4405054/3‑150 (MQ=255)
  gcgTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtt                                                                                                                                                  <  1:3230743/150‑1 (MQ=255)
       ttGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGa                                                                                                                                             >  2:1286500/1‑150 (MQ=255)
          cTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATa                                                                                                                                          <  2:2559777/150‑1 (MQ=255)
           tCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAg                                                                                                                                         <  1:2592351/150‑1 (MQ=255)
           tCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAg                                                                                                                                         <  1:440237/150‑1 (MQ=255)
             ggCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATGTGGTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCt                                                                                                                                       <  1:2381102/150‑1 (MQ=255)
              gCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTc                                                                                                                                      <  1:3314780/150‑1 (MQ=255)
              gCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTc                                                                                                                                      >  1:1797957/1‑150 (MQ=255)
               cAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCg                                                                                                                                     <  2:4171527/150‑1 (MQ=255)
               cAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCg                                                                                                                                     <  1:4296847/150‑1 (MQ=255)
                 aTTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGc                                                                                                                                   >  1:533025/1‑150 (MQ=255)
                 aTTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGc                                                                                                                                   >  1:1419136/1‑150 (MQ=255)
                 aTTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGc                                                                                                                                   >  2:5057642/1‑150 (MQ=255)
                   tGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCaa                                                                                                                                 <  2:3817436/150‑1 (MQ=255)
                            gAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCt                                                                                                                        >  2:1253215/1‑150 (MQ=255)
                             aaGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTa                                                                                                                       >  1:2929952/1‑150 (MQ=255)
                              attAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTAc                                                                                                                      >  1:3448846/3‑150 (MQ=255)
                              aGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTAc                                                                                                                      <  1:1283181/150‑1 (MQ=255)
                                tAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACtt                                                                                                                    >  1:1312919/1‑150 (MQ=255)
                                   cGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTc                                                                                                                 <  2:4657569/150‑1 (MQ=255)
                                    gATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCg                                                                                                                >  2:1846288/1‑150 (MQ=255)
                                       ccGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCa                                                                                                             >  1:2993822/1‑150 (MQ=255)
                                             aTTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaac                                                                                                       <  2:3599407/150‑1 (MQ=255)
                                              tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAAcc                                                                                                      <  2:3137739/150‑1 (MQ=255)
                                               ttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCg                                                                                                     >  1:1993209/1‑150 (MQ=255)
                                               ttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCg                                                                                                     >  2:1508462/1‑150 (MQ=255)
                                                tACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGc                                                                                                    >  1:2621572/1‑150 (MQ=255)
                                                  cGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTg                                                                                                  >  1:942236/1‑150 (MQ=255)
                                                   gTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCAAAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTgg                                                                                                 <  1:254962/150‑1 (MQ=255)
                                                    tGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGa                                                                                                <  1:3270118/150‑1 (MQ=255)
                                                    tGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGa                                                                                                >  1:4643827/1‑150 (MQ=255)
                                                       aaaCTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAggg                                                                                             >  1:3975534/1‑150 (MQ=255)
                                                           tCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCa                                                                                         <  2:2830254/150‑1 (MQ=255)
                                                            cGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCaa                                                                                        >  2:2719126/1‑150 (MQ=255)
                                                              aCCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAt                                                                                      <  1:4053153/150‑1 (MQ=255)
                                                              aCCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAt                                                                                      >  2:1062418/1‑150 (MQ=255)
                                                               ccATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAtt                                                                                     >  2:3953110/1‑150 (MQ=255)
                                                                 aTGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTa                                                                                   >  1:1365020/1‑150 (MQ=255)
                                                                   gCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAgg                                                                                 <  2:2469995/150‑1 (MQ=255)
                                                                      ggAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCAc                                                                              <  1:599504/150‑1 (MQ=255)
                                                                       gAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACg                                                                             <  2:3053919/150‑1 (MQ=255)
                                                                          ccTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCg                                                                          >  1:2808010/1‑150 (MQ=255)
                                                                               aCGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGaa                                                                     <  2:5243177/150‑1 (MQ=255)
                                                                                cGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGaaa                                                                    >  2:5325320/1‑150 (MQ=255)
                                                                                   aTCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTg                                                                 <  1:4966913/150‑1 (MQ=255)
                                                                                   aTCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTg                                                                 <  1:1768675/150‑1 (MQ=255)
                                                                                   aTCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTg                                                                 >  2:851984/1‑150 (MQ=255)
                                                                                    tCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGa                                                                >  2:1968105/1‑150 (MQ=255)
                                                                                    tCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGa                                                                >  2:1741827/1‑150 (MQ=255)
                                                                                     cGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGAt                                                               <  1:4173111/150‑1 (MQ=255)
                                                                                      gCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACATCCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATc                                                              <  2:4596109/150‑1 (MQ=255)
                                                                                      gCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATc                                                              >  1:5179205/1‑150 (MQ=255)
                                                                                      gCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATc                                                              <  2:3464282/150‑1 (MQ=255)
                                                                                       ccGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCg                                                             <  2:4939276/150‑1 (MQ=255)
                                                                                        cGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCgg                                                            >  1:5104199/1‑150 (MQ=255)
                                                                                         ggAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCggg                                                           >  2:24439/1‑150 (MQ=255)
                                                                                         ggAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCggg                                                           <  1:421160/150‑1 (MQ=255)
                                                                                           aGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTc                                                         <  2:2944904/150‑1 (MQ=255)
                                                                                              aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttt                                                      >  1:1712332/1‑150 (MQ=255)
                                                                                                  gCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCt                                                  >  1:3777125/1‑150 (MQ=255)
                                                                                                  gCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCt                                                  <  1:1476906/150‑1 (MQ=255)
                                                                                                    cATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGc                                                >  2:4421963/1‑150 (MQ=255)
                                                                                                    cATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGc                                                >  2:4972281/1‑150 (MQ=255)
                                                                                                     aTCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGcc                                               >  1:3183627/1‑150 (MQ=255)
                                                                                                            tgttgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCAt                                        <  2:743159/150‑1 (MQ=255)
                                                                                                            tgttgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCAt                                        <  2:4128283/150‑1 (MQ=255)
                                                                                                             gttgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATa                                       <  1:2182745/150‑1 (MQ=255)
                                                                                                              ttgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATaa                                      >  2:1997090/1‑150 (MQ=255)
                                                                                                                gtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAAcc                                    <  2:2912636/150‑1 (MQ=255)
                                                                                                                 tCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCa                                   >  1:1164550/1‑150 (MQ=255)
                                                                                                                       gCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCt                             >  1:3553009/1‑150 (MQ=255)
                                                                                                                        cTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtt                            <  2:2262360/150‑1 (MQ=255)
                                                                                                                        cTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtt                            >  2:3563539/1‑150 (MQ=255)
                                                                                                                         tGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCttt                           >  1:478240/1‑150 (MQ=255)
                                                                                                                         tGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCttt                           >  1:722909/1‑150 (MQ=255)
                                                                                                                          gCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtttt                          <  2:1938365/150‑1 (MQ=255)
                                                                                                                            cGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtttttt                        >  2:565298/1‑150 (MQ=255)
                                                                                                                             gTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTg                       <  2:155195/150‑1 (MQ=255)
                                                                                                                             gTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTg                       >  2:4854691/1‑150 (MQ=255)
                                                                                                                               ttCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCTAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGATTGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGt                     <  1:3373679/150‑1 (MQ=255)
                                                                                                                                 cGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCGGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCa                   >  2:1422564/1‑150 (MQ=255)
                                                                                                                                   cacaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATg                 <  2:897052/150‑1 (MQ=255)
                                                                                                                                   cacaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATg                 >  1:1612282/1‑150 (MQ=255)
                                                                                                                                   cacaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATg                 <  2:1614026/150‑1 (MQ=255)
                                                                                                                                    acaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGa                >  2:2871954/1‑150 (MQ=255)
                                                                                                                                    acaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGa                >  1:4868891/1‑150 (MQ=255)
                                                                                                                                     caACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGat               <  2:821016/150‑1 (MQ=255)
                                                                                                                                        ccAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATg            <  2:176777/150‑1 (MQ=255)
                                                                                                                                          aGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCa          >  1:3764640/1‑150 (MQ=255)
                                                                                                                                              gCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGaa      >  1:786895/1‑150 (MQ=255)
                                                                                                                                               ccAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAAc     >  2:4114909/1‑150 (MQ=255)
                                                                                                                                               ccAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAAc     >  2:1320073/1‑150 (MQ=255)
                                                                                                                                                cAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACt    >  2:4030950/1‑150 (MQ=255)
                                                                                                                                                cAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACt    >  1:1662171/1‑150 (MQ=255)
                                                                                                                                                cAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACt    <  1:1823048/150‑1 (MQ=255)
                                                                                                                                                 aaaGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTg   <  2:3285371/150‑1 (MQ=255)
                                                                                                                                                  aaGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTGc  >  1:3924151/1‑150 (MQ=255)
                                                                                                                                                  aaGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTGc  <  2:11601/150‑1 (MQ=255)
                                                                                                                                                  aaGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTGc  >  1:1565898/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCGCGTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTGC  >  CP009273/434514‑434809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑