Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,336 G→T 100% Q100K (CAG→AAG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,3360GT100.0% 897.2 / NA 272Q100K (CAG→AAG) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (126/146);  total (126/146)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 272 total aligned reads displayed.

CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAGG  >  CP009273/435189‑435482
                                                                                                                                                   |                                                                                                                                                  
     aCGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATg                                                                                                                                             <  2:4590762/150‑1 (MQ=255)
      cGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATgg                                                                                                                                            <  1:180063/150‑1 (MQ=255)
          gcggcgATTTTTGCCTACTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTTATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGTGTGCAGACCGCCTGTCTTACGGATGGTGcc                                                                                                                                        >  2:541350/1‑150 (MQ=255)
            ggcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGa                                                                                                                                      >  2:1304847/1‑150 (MQ=255)
               gATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAttt                                                                                                                                   <  2:1909362/150‑1 (MQ=255)
                aTTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGTATGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAtttt                                                                                                                                  >  1:3022947/1‑150 (MQ=255)
                aTTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAtttt                                                                                                                                  <  2:3130275/150‑1 (MQ=255)
                aTTTTTGCCTTATTTTTAGGCAGCAAACGCAATACCAATTCCGGCACAGATTGATGTTGATGAATTCCCGACGCTTAAAACGTCATATTCGCTTTCGCCGCCCCACGGGAACGGGTGCAGACCGCCTTTATTACGGAAGGTGCCGAtttt                                                                                                                                  >  2:5103859/1‑150 (MQ=255)
                     tGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcgc                                                                                                                             >  1:3341788/1‑150 (MQ=255)
                      gCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcgcg                                                                                                                            <  1:2826501/150‑1 (MQ=255)
                       ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGACCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcgg                                                                                                                           >  1:370879/1‑150 (MQ=255)
                        cttcttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGACCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcggc                                                                                                                          >  2:764660/1‑150 (MQ=255)
                        cttcttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcggc                                                                                                                          <  1:2056034/150‑1 (MQ=255)
                        cttcttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTGTCTTACGGAGGGTGCCGATTTTGTCgcggc                                                                                                                          >  2:3091020/1‑150 (MQ=255)
                         ttcttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcggcg                                                                                                                         >  2:2073467/1‑150 (MQ=255)
                         ttcttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcggcg                                                                                                                         >  1:678822/1‑150 (MQ=255)
                            tttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTcc                                                                                                                      <  2:3718737/150‑1 (MQ=255)
                            tttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTcc                                                                                                                      <  1:3393396/150‑1 (MQ=255)
                                   gcagcaACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCaaa                                                                                                               <  2:4177006/150‑1 (MQ=255)
                                   gcagcaACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCaaa                                                                                                               <  2:5257222/150‑1 (MQ=255)
                                        aaCCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAtttt                                                                                                          >  2:2190766/1‑150 (MQ=255)
                                           cGCAATACCAATTCCGGCACTGATGGAGGTTGATGTATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGATCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                       <  2:451430/150‑1 (MQ=255)
                                           cGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                       <  2:4087914/150‑1 (MQ=255)
                                           cGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                       >  1:3479390/1‑150 (MQ=255)
                                               aTACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGa                                                                                                   >  2:4438028/1‑150 (MQ=255)
                                                tACCAATTCTGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGAt                                                                                                  <  1:4967163/150‑1 (MQ=255)
                                                  ccAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATaa                                                                                                >  2:1417064/1‑150 (MQ=255)
                                                   cAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                               <  1:1914272/150‑1 (MQ=255)
                                                        ccGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGa                                                                                          <  2:582567/150‑1 (MQ=255)
                                                         cGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGTTGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGAt                                                                                         <  1:4690133/150‑1 (MQ=255)
                                                         cGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGAt                                                                                         <  1:4182011/150‑1 (MQ=255)
                                                         cGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGAt                                                                                         >  1:2304033/1‑150 (MQ=255)
                                                          ggCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                        <  2:2897839/150‑1 (MQ=255)
                                                            cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCTATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcc                                                                                      >  1:1771785/1‑150 (MQ=255)
                                                            cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcc                                                                                      <  1:4664559/150‑1 (MQ=255)
                                                             aCTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGccc                                                                                     <  1:3458528/150‑1 (MQ=255)
                                                                 aTGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                                 >  2:1832043/1‑150 (MQ=255)
                                                                 aTGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                                 <  1:1037909/150‑1 (MQ=255)
                                                                 aTGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                                 >  2:2758790/1‑150 (MQ=255)
                                                                 aTGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCACCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                                 <  1:1224486/150‑1 (MQ=255)
                                                                      ggTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCa                                                                            >  1:4040549/1‑150 (MQ=255)
                                                                       gTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCaa                                                                           >  1:2973892/1‑150 (MQ=255)
                                                                        ttGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCaaa                                                                          <  1:1386681/150‑1 (MQ=255)
                                                                         tgatgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAAt                                                                         <  2:5151861/150‑1 (MQ=255)
                                                                          gatgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATc                                                                        >  2:2233521/1‑150 (MQ=255)
                                                                          gatgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATc                                                                        >  2:1790814/1‑150 (MQ=255)
                                                                          gatgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATc                                                                        >  2:1623022/1‑150 (MQ=255)
                                                                           atgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCa                                                                       <  2:2445723/150‑1 (MQ=255)
                                                                           atgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCa                                                                       >  1:1079291/1‑150 (MQ=255)
                                                                                tGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTgg                                                                  >  1:5276677/1‑150 (MQ=255)
                                                                                    cGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaa                                                              >  2:3684843/1‑150 (MQ=255)
                                                                                     gACGATTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaaa                                                             >  2:4053317/1‑150 (MQ=255)
                                                                                      aCGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAAc                                                            >  2:1722087/1‑150 (MQ=255)
                                                                                       cGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACg                                                           >  1:503324/1‑150 (MQ=255)
                                                                                       cGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACg                                                           <  1:1163229/150‑1 (MQ=255)
                                                                                         cTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACggg                                                         >  1:938726/1‑150 (MQ=255)
                                                                                           tAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAATCCTGATGCCCCACATCCCACATCAATTGGTCAAACGGGgt                                                       >  2:3816218/1‑150 (MQ=255)
                                                                                           tAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgt                                                       <  2:1212812/150‑1 (MQ=255)
                                                                                             aTACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtgt                                                     >  1:1020404/1‑150 (MQ=255)
                                                                                              tACGTCATATTCGCTTTCGCCGCGCCACGTGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGtgtt                                                    <  2:529063/150‑1 (MQ=255)
                                                                                              tACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGtgtt                                                    <  2:674668/150‑1 (MQ=255)
                                                                                               aCGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGtgttg                                                   <  2:4736454/150‑1 (MQ=255)
                                                                                                cGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGtgttgt                                                  <  1:4266796/150‑1 (MQ=255)
                                                                                                 gTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTa                                                 <  2:345093/150‑1 (MQ=255)
                                                                                                    atatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                              >  2:3323322/1‑150 (MQ=255)
                                                                                                     tatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGATCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACa                                             <  1:4428949/150‑1 (MQ=255)
                                                                                                     tatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACa                                             >  1:3920302/1‑150 (MQ=255)
                                                                                                      atTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACAt                                            <  2:4261246/150‑1 (MQ=255)
                                                                                                       ttCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATa                                           <  2:3046830/150‑1 (MQ=255)
                                                                                                        tCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAg                                          <  2:3629439/150‑1 (MQ=255)
                                                                                                        tCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAg                                          >  2:720313/1‑150 (MQ=255)
                                                                                                        tCGCTTTAGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACTGATGGTTCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGACTGATGCCCAACATACCAAATCAATTGTGCAAACGGGGTTTTGTAGACATAg                                          >  1:2475264/1‑150 (MQ=255)
                                                                                                          gCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTg                                        <  1:1518511/150‑1 (MQ=255)
                                                                                                          gCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTg                                        <  1:379522/150‑1 (MQ=255)
                                                                                                          gCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTg                                        <  2:105415/150‑1 (MQ=255)
                                                                                                           cTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATTGTGc                                       <  1:4598927/150‑1 (MQ=255)
                                                                                                           cTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGc                                       >  1:3163256/1‑150 (MQ=255)
                                                                                                           cTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGc                                       >  1:4086578/1‑150 (MQ=255)
                                                                                                           cTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGc                                       >  2:1553238/1‑150 (MQ=255)
                                                                                                           cTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGc                                       <  2:1699058/150‑1 (MQ=255)
                                                                                                              tCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAgc                                    <  2:448746/150‑1 (MQ=255)
                                                                                                                  cgcgCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCa                                >  1:2710346/1‑150 (MQ=255)
                                                                                                                    cgcCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACg                              >  2:484812/1‑150 (MQ=255)
                                                                                                                    cgcCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACg                              <  2:2577365/150‑1 (MQ=255)
                                                                                                                     gcCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACgg                             >  2:49035/1‑150 (MQ=255)
                                                                                                                     gcCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACgg                             <  1:340202/150‑1 (MQ=255)
                                                                                                                       cACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCTAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTc                           <  1:5212144/150‑1 (MQ=255)
                                                                                                                        aCGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCa                          <  2:815575/150‑1 (MQ=255)
                                                                                                                         cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                         <  2:1995933/150‑1 (MQ=255)
                                                                                                                          gggAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                        >  1:3345137/1‑150 (MQ=255)
                                                                                                                          gggAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                        >  1:557276/1‑150 (MQ=255)
                                                                                                                            gAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTc                      <  1:3988620/150‑1 (MQ=255)
                                                                                                                                gggTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGAcc                  >  2:3570809/1‑150 (MQ=255)
                                                                                                                                   tGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGTATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTg               <  2:3022947/150‑1 (MQ=255)
                                                                                                                                     cAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGcc             >  1:2358218/1‑150 (MQ=255)
                                                                                                                                             cTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCgg     >  2:2516238/1‑150 (MQ=255)
                                                                                                                                              tttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGa    >  1:1591024/1‑150 (MQ=255)
                                                                                                                                              tttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGa    <  1:1715580/150‑1 (MQ=255)
                                                                                                                                               ttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCACTTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAg   <  1:757950/150‑1 (MQ=255)
                                                                                                                                                tCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAgg  <  2:4561511/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAGG  >  CP009273/435189‑435482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑