Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 446,369 T→G 100% Q233P (CAG→CCG)  cyoA ← cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273446,3690TG98.7% 1136.5 / ‑1.1 290Q233P (CAG→CCG) cyoAcytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base G (136/150);  total (137/153)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 297 total aligned reads displayed.

ATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCA  >  CP009273/446222‑446515
                                                                                                                                                   |                                                                                                                                                  
aTAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACggg                                                                                                                                                  >  1:4533957/1‑150 (MQ=255)
aTAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACggg                                                                                                                                                  <  2:2580682/150‑1 (MQ=255)
aTAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACggg                                                                                                                                                  <  2:435087/150‑1 (MQ=255)
aTAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACggg                                                                                                                                                  >  2:4103756/1‑150 (MQ=255)
aTAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGACCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACggg                                                                                                                                                  >  1:3840994/1‑150 (MQ=255)
     cTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                             >  1:3943479/1‑150 (MQ=255)
     cTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                             <  1:607532/150‑1 (MQ=255)
     cTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCGGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                             >  2:2474732/1‑150 (MQ=255)
       tGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATg                                                                                                                                           <  2:4604209/150‑1 (MQ=255)
           aaTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcggc                                                                                                                                       >  2:4255903/1‑150 (MQ=255)
           aaTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcggc                                                                                                                                       >  1:5189936/1‑150 (MQ=255)
            aTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcggcg                                                                                                                                      <  2:4869105/150‑1 (MQ=255)
             ttACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGgcgc                                                                                                                                     <  1:1775027/150‑1 (MQ=255)
             ttACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGgcgc                                                                                                                                     <  1:1316662/150‑1 (MQ=255)
               aCATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGa                                                                                                                                   >  2:3474988/1‑150 (MQ=255)
                cATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGAt                                                                                                                                  >  2:3206601/1‑150 (MQ=255)
                cATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGAt                                                                                                                                  >  2:3371004/1‑150 (MQ=255)
                 aTCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATc                                                                                                                                 <  1:4377590/150‑1 (MQ=255)
                 aTCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATc                                                                                                                                 >  1:236621/1‑150 (MQ=255)
                    ggCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCgg                                                                                                                              >  2:2429502/1‑150 (MQ=255)
                       aaaCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGgtgt                                                                                                                           >  2:1562361/1‑150 (MQ=255)
                       aaaCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGGGGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCCCGGGTCGAATGCGGCGCGATCCGgtgt                                                                                                                           >  2:2203628/1‑150 (MQ=255)
                       aaaCAAGTCTGGTTTCACGTTGGAGAAATAGTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGgtgt                                                                                                                           <  2:3944015/150‑1 (MQ=255)
                        aaCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGtt                                                                                                                          <  1:1647584/150‑1 (MQ=255)
                             gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAAt                                                                                                                     <  1:2316763/150‑1 (MQ=255)
                              tCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATa                                                                                                                    <  2:91697/150‑1 (MQ=255)
                               cTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAg                                                                                                                   <  1:2728326/150‑1 (MQ=255)
                               cTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAg                                                                                                                   <  1:1254612/150‑1 (MQ=255)
                                 ggTTTCACGTTGGAGAAATATTCCACCTGGTTGTAGTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCt                                                                                                                 <  2:1007230/150‑1 (MQ=255)
                                  gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCtt                                                                                                                >  2:3154260/1‑150 (MQ=255)
                                   tttCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCttt                                                                                                               <  1:5091347/150‑1 (MQ=255)
                                    ttCACGTTGGAGAAATAGTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTg                                                                                                              <  2:4829974/150‑1 (MQ=255)
                                     tCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGa                                                                                                             <  1:1894565/150‑1 (MQ=255)
                                      cACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGaa                                                                                                            >  2:376914/1‑150 (MQ=255)
                                       aCGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAAc                                                                                                           <  2:1190782/150‑1 (MQ=255)
                                        cGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACt                                                                                                          <  1:746811/150‑1 (MQ=255)
                                        cGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACt                                                                                                          <  1:930739/150‑1 (MQ=255)
                                         gTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCGTTGAACtt                                                                                                         >  2:827250/1‑150 (MQ=255)
                                          ttGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTc                                                                                                        <  1:1341582/150‑1 (MQ=255)
                                            ggAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCAt                                                                                                      >  1:2011925/1‑150 (MQ=255)
                                               gaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGcc                                                                                                   >  1:1528374/1‑150 (MQ=255)
                                                  atatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTga                                                                                                >  2:3649693/1‑150 (MQ=255)
                                                  atatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTga                                                                                                <  1:2941693/150‑1 (MQ=255)
                                                  atatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTga                                                                                                <  1:1211546/150‑1 (MQ=255)
                                                   tatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgag                                                                                               <  2:618429/150‑1 (MQ=255)
                                                    atTCCACCTGGTTGTTTTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATTCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATTCCAgaga                                                                                              >  1:4051911/1‑150 (MQ=255)
                                                    atTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCGATGTCAGTCATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgaga                                                                                              <  1:3331467/150‑1 (MQ=255)
                                                      tCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAg                                                                                            <  2:2665227/150‑1 (MQ=255)
                                                      tCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAg                                                                                            <  1:3184434/150‑1 (MQ=255)
                                                            tGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCggg                                                                                      <  2:2695783/150‑1 (MQ=255)
                                                            tGGGTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCggg                                                                                      >  2:2914791/1‑150 (MQ=255)
                                                             ggTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGc                                                                                     >  1:4954484/1‑150 (MQ=255)
                                                             ggTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGc                                                                                     >  1:3429813/1‑150 (MQ=255)
                                                                     tCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAg                                                                             <  1:2223249/150‑1 (MQ=255)
                                                                        cTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTg                                                                          >  2:4765500/1‑150 (MQ=255)
                                                                        cTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTg                                                                          <  1:1032345/150‑1 (MQ=255)
                                                                         tAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTgg                                                                         >  1:1056456/1‑150 (MQ=255)
                                                                            gcgcGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGACCTTCCTGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCgg                                                                      <  2:593593/150‑1 (MQ=255)
                                                                                ggCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAt                                                                  >  2:4995112/1‑150 (MQ=255)
                                                                                ggCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAt                                                                  <  2:2032774/150‑1 (MQ=255)
                                                                                ggCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAt                                                                  <  2:5179002/150‑1 (MQ=255)
                                                                                 gCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATa                                                                 <  2:1844722/150‑1 (MQ=255)
                                                                                  ccAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATAc                                                                <  1:268276/150‑1 (MQ=255)
                                                                                    aGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCg                                                              >  1:3766002/1‑150 (MQ=255)
                                                                                    aGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCg                                                              >  2:142494/1‑150 (MQ=255)
                                                                                        tttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCAt                                                          <  2:3874900/150‑1 (MQ=255)
                                                                                         ttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATa                                                         >  2:1620513/1‑150 (MQ=255)
                                                                                           cGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAg                                                       <  2:1090629/150‑1 (MQ=255)
                                                                                            gAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGt                                                      >  1:4470034/1‑150 (MQ=255)
                                                                                             aaCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATAACGTAATAAGTg                                                     >  2:684471/1‑150 (MQ=255)
                                                                                                gCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCg                                                  >  1:4750042/1‑150 (MQ=255)
                                                                                                  aGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCggg                                                >  1:3975765/1‑150 (MQ=255)
                                                                                                   gCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGt                                               >  2:2354760/1‑150 (MQ=255)
                                                                                                     cATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTc                                             <  1:2118912/150‑1 (MQ=255)
                                                                                                     cATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTc                                             >  1:1753446/1‑150 (MQ=255)
                                                                                                      aTGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCg                                            >  1:3276429/1‑150 (MQ=255)
                                                                                                      aTGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCg                                            >  2:4434863/1‑150 (MQ=255)
                                                                                                         tCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTg                                         <  1:3225272/150‑1 (MQ=255)
                                                                                                         tCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTg                                         >  2:4123319/1‑150 (MQ=255)
                                                                                                           aGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGc                                       >  1:4876022/1‑150 (MQ=255)
                                                                                                            gACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCg                                      >  2:1983281/1‑150 (MQ=255)
                                                                                                              cATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGAt                                    >  2:1589884/1‑150 (MQ=255)
                                                                                                                tGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCa                                  >  2:3430838/1‑150 (MQ=255)
                                                                                                                tGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCa                                  <  1:3680685/150‑1 (MQ=255)
                                                                                                                    gtTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGCTCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATg                              <  2:454743/150‑1 (MQ=255)
                                                                                                                    gtTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATg                              <  2:368532/150‑1 (MQ=255)
                                                                                                                           gACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGa                       >  1:3605705/1‑150 (MQ=255)
                                                                                                                            aCTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAg                      >  2:1075331/1‑150 (MQ=255)
                                                                                                                               gCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATGAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCt                   <  2:1784095/150‑1 (MQ=255)
                                                                                                                               gCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCt                   >  2:1684684/1‑150 (MQ=255)
                                                                                                                               gCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCt                   <  1:4191428/150‑1 (MQ=255)
                                                                                                                                 ttCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGc                 <  1:1112077/150‑1 (MQ=255)
                                                                                                                                 ttCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGc                 <  1:4287521/150‑1 (MQ=255)
                                                                                                                                    gCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATa              <  1:5246229/150‑1 (MQ=255)
                                                                                                                                      ttttGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATAcc            >  1:2451484/1‑150 (MQ=255)
                                                                                                                                      ttttGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATAcc            >  1:184701/1‑150 (MQ=255)
                                                                                                                                          gCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGcc        <  1:731924/150‑1 (MQ=255)
                                                                                                                                           cGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCa       >  2:4142505/1‑150 (MQ=255)
                                                                                                                                            gACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCAt      <  1:300864/150‑1 (MQ=255)
                                                                                                                                             aCCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATg     <  1:2922697/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCA  >  CP009273/446222‑446515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑