Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA99.9% 1036.1 / ‑6.7 319intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (150/163);  minor base T (0/4);  total (150/169)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 321 total aligned reads displayed.

TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG  >  CP009273/450143‑450436
                                                                                                                                                   |                                                                                                                                                     
tCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAt                                                                                                                                                     <  1:3513001/150‑1 (MQ=255)
    gcgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTa                                                                                                                                                 >  1:4764969/1‑150 (MQ=255)
     cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAt                                                                                                                                                <  2:4569001/150‑1 (MQ=255)
       cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                              >  1:515882/1‑150 (MQ=255)
        tGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaa                                                                                                                                             >  2:2177798/1‑150 (MQ=255)
          gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                           >  2:813161/1‑150 (MQ=255)
           ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                          <  2:126049/150‑1 (MQ=255)
            tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc                                                                                                                                         <  1:4024008/150‑1 (MQ=255)
               gCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                      >  1:4868669/1‑150 (MQ=255)
                 aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                    >  2:4003184/1‑150 (MQ=255)
                  tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTg                                                                                                                                   >  1:2165920/1‑150 (MQ=255)
                   aCTTACACTATTAAGGTGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCTTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGc                                                                                                                                  <  1:3128286/150‑1 (MQ=255)
                     ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCgg                                                                                                                                >  1:4151247/1‑150 (MQ=255)
                        cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGcc                                                                                                                             <  2:5185156/150‑1 (MQ=255)
                           tattatGGTGTGGGACGGGTTGCAGGACACCGTCTTTGCCTCTCCCCCCTGTCGTGGAGCTGGAAGCATCGCGTAAAAACGAATTGGCACCTGTCGGCGCTTTTCCTGTATGTTGCTAAATATTTTATGAAAAACGGCCTGCGGGCCgt‑t                                                                                                                         <  1:3722372/150‑1 (MQ=255)
                            aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tg                                                                                                                        >  1:5116789/1‑149 (MQ=255)
                               aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttgt                                                                                                                     >  2:2487753/1‑150 (MQ=255)
                               aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttgt                                                                                                                     >  1:854424/1‑150 (MQ=255)
                                 gtaGTGGGATGGGTTGCAGTACACCTTCTTGGCCTCTCCTCCCTGTCTTGGAGCAGGAAGCATCGCGTAAAAACTCATTTGAAACTGTCGGCGCTTTTCAAGTATGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCgt‑tttgttt                                                                                                                   <  1:902955/148‑1 (MQ=255)
                                  gAGGGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCACCGGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttgtttt                                                                                                                  <  1:1836891/150‑1 (MQ=255)
                                   aGTGGTAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAAGGCCTGCGGGCCgt‑tttgttttt                                                                                                                 >  2:4352912/1‑149 (MQ=255)
                                   aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGt‑tttgttttg                                                                                                                 >  2:4233779/1‑150 (MQ=255)
                                   aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGt‑tttgttttg                                                                                                                 >  1:1303093/1‑150 (MQ=255)
                                    gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑tttgttttgt                                                                                                                >  2:1078810/1‑150 (MQ=255)
                                     tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTc                                                                                                               <  1:1614443/150‑1 (MQ=255)
                                      gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑tttgttttgtat                                                                                                              >  1:4378057/1‑148 (MQ=255)
                                        gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GGTGTTTTGTCT‑gg                                                                                                           >  2:2936516/1‑150 (MQ=255)
                                         aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCT‑GGa                                                                                                          >  1:3976157/1‑150 (MQ=255)
                                         aaGGGTAGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCAGT‑TTTTTTTTTTAT‑GGa                                                                                                          >  1:3458398/1‑150 (MQ=255)
                                          aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGAt                                                                                                         <  2:106125/150‑1 (MQ=255)
                                            ggTTGCCGGACACCGTCTTGGCCTCACCTCCCTGTCGTGTAGCAGGAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCATTATGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGAttt                                                                                                       <  1:4473986/150‑1 (MQ=255)
                                            ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGAttt                                                                                                       <  1:3932869/150‑1 (MQ=255)
                                             gTTGCAGGACACCGTCTTTGCCACTCCGCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGAtttt                                                                                                      <  1:4537941/150‑1 (MQ=255)
                                              ttGCTGGACACCGTGTTTGCCTCTCCTCCGTGTCGTGGAGCAGGAAGCATCTCGTATTTTGGCTTTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAATATTTTTTTGAAAATCGGCCTGCGTGCCGT‑TTTGTTTTGTCT‑GGATTTTg                                                                                                     <  2:1527707/150‑1 (MQ=255)
                                                gCAGGACACCTTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTgcg                                                                                                   <  1:2737727/150‑1 (MQ=255)
                                                gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTTCT‑GGATTTTgcg                                                                                                   >  2:36303/1‑150 (MQ=255)
                                                 cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑GTTGTTTTGTGT‑GGGTTTTgggc                                                                                                  >  2:2377381/1‑150 (MQ=255)
                                                 cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCAG‑TTTGTTTTGTCT‑GGATTTTgcgc                                                                                                  >  1:3346605/1‑150 (MQ=255)
                                                        ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGc                                                                                           <  2:369385/150‑1 (MQ=255)
                                                        ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGGTTTTTGG‑GGTTTTTTGGCttttttg                                                                                           >  1:1523520/1‑148 (MQ=255)
                                                         cGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCG‑GGATTTTGCGCTTTTTGcc                                                                                          <  2:3189977/150‑1 (MQ=255)
                                                          gTCTTTGCCTCTCCTCGCTGTCGTGGTGCAGTAAGCATCGAGTAAAAACGATTTTGCAACTGTCGGCGCTTTTCCTTTTTTTTGCTAAAATTTTTTGGAAATACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGccc                                                                                         <  1:3216080/150‑1 (MQ=255)
                                                          gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTCTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGTGGGTTTTTGGGGTTTTTGcc                                                                                          >  1:1106426/1‑150 (MQ=255)
                                                          gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATGTTATGAAAAACGGCCTGCGGGCCGT‑TTGGTTTTGTCT‑GGATTTTGCGCTTTTTGccc                                                                                         >  2:735166/1‑150 (MQ=255)
                                                          gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTGT‑GGATTTTGCGCTTTTTGcac                                                                                         >  2:2017587/1‑148 (MQ=255)
                                                          gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCT‑GGATTTTTCGCTTTTTTccc                                                                                         >  2:5327384/1‑150 (MQ=255)
                                                            cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAg                                                                                       <  2:409923/150‑1 (MQ=255)
                                                              ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCa                                                                                     >  2:4390102/1‑150 (MQ=255)
                                                                 ccTCTCCTCCCTGTCGTGGAGCTGGAAGCATCTCGTAAAAACGCATTTGCATCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTc                                                                                  <  2:4904850/150‑1 (MQ=255)
                                                                  ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                 >  2:2918707/1‑150 (MQ=255)
                                                                  ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGT‑GGATTTTTCGCGTTTTGCCCAGccataa                                                                                 >  1:1215264/1‑145 (MQ=255)
                                                                           cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTAT‑GGATTTTTAGCTTTTTTGCCCGCAATAAG‑ACAaaaaa                                                                       >  1:2861520/1‑149 (MQ=255)
                                                                            tGTCGTGGAGCAGGTAGCATCGCGTAAAATCTCTTTTGCTCCTGTCGGGGCTTTTACAGTTTGTTGATAAATTTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTTCGCTTTTTGCCCAGCATTCAG‑ACGAAAAtt                                                                      <  1:3772083/150‑1 (MQ=255)
                                                                             gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTg                                                                     <  2:3371849/150‑1 (MQ=255)
                                                                                gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGccc                                                                  <  1:665903/150‑1 (MQ=255)
                                                                                  ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCgg                                                                >  2:3430372/1‑150 (MQ=255)
                                                                                      cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTGT‑GGATTTTTAGCTTTTTTCCCAGCATTCAG‑ACGAAAATTTCCCGGGAAt                                                            >  2:3284037/1‑150 (MQ=255)
                                                                                      cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAAt                                                            <  2:5083197/150‑1 (MQ=255)
                                                                                      cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTAAG‑ACGAAAATTGCCCGGGAAt                                                            >  2:3798660/1‑150 (MQ=255)
                                                                                       aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAAtt                                                           <  2:1720132/150‑1 (MQ=255)
                                                                                       aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGT‑GGTTTTTTGGCTTTTTTGCCAGCAATGAG‑TCGGAAATTGGCCggggagt                                                           >  2:4467123/1‑146 (MQ=255)
                                                                                        ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTTTTTTTTGT‑GGGTTTTTCGCTTTTTTCCCACCATTAAG‑TCGAAAATTTACCGGGAAttt                                                          >  1:792534/1‑149 (MQ=255)
                                                                                        ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGT‑GGGTTTTGCGCTTTTTTCCCAGCATTACT‑CAGAAAAATGCCCggggagtg                                                          >  2:4027222/1‑145 (MQ=255)
                                                                                         gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTGT‑GGGTTTTGGGGTTTTTTGCCCGCAATAAGAACAAAAATTGCCGGGGGATtg                                                          >  1:1004064/1‑150 (MQ=255)
                                                                                          aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATtgtg                                                        <  2:5097530/150‑1 (MQ=255)
                                                                                           aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAATCGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGa                                                       >  2:2641175/1‑150 (MQ=255)
                                                                                           aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGACTTGTGa                                                       >  2:2770670/1‑150 (MQ=255)
                                                                                            gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGaa                                                      <  2:3850716/150‑1 (MQ=255)
                                                                                              aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGaaaa                                                    >  2:3478838/1‑150 (MQ=255)
                                                                                                 gcttAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                 <  1:4107832/147‑1 (MQ=255)
                                                                                                 gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                 <  1:507173/150‑1 (MQ=255)
                                                                                                 gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑GTTGTTTTTTGG‑GGTTTTTTGGCTTTTTGCCCAGCCATCAG‑ACGAAAATTGGCCGGGGAGTGTGAAAAAAt                                                 >  1:341689/1‑150 (MQ=255)
                                                                                                  cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGAGCTTTTTGCCCAGCAATAAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATa                                                >  1:1720124/1‑150 (MQ=255)
                                                                                                   gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATAc                                               <  1:3924830/150‑1 (MQ=255)
                                                                                                   gTAAAAACGATTTTGCAACTGTCGGCGCTTTTCCTGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATAc                                               <  1:2297226/150‑1 (MQ=255)
                                                                                                      aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                            <  1:5281834/150‑1 (MQ=255)
                                                                                                       aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑TGTGTTTTTTGT‑GGATTTTTGGCTTTTTGGCCAGCAATCAG‑ACGAAAAATGGCCGGGAAATTTGGAAAAATAcgcgg                                           >  2:1359318/1‑149 (MQ=255)
                                                                                                        aaCGCATTTGCAACTGTCGGCGCTTGTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGAc                                          <  2:3866452/150‑1 (MQ=255)
                                                                                                            cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGT‑GGTTTTTTGGCTTTTTGCCCCGCAATCAG‑TCGAAAAATTGCCGGGAAGTGTGTAAAAATACACGAAAgcg                                      >  2:4509588/1‑150 (MQ=255)
                                                                                                              tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                    <  2:4764969/150‑1 (MQ=255)
                                                                                                                  cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGAG‑GGATTTTGGGTTTTTTGCCCACCATAAAA‑ACAAAAATTG‑CCGGGGATTTTTAAAAAATAAACCACAgggcgccca                                >  1:569648/1‑147 (MQ=255)
                                                                                                                   aagtgtgGGCGATTTTCCAGTATTTTGATAAAAATTTTATGAAACACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGAATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                               <  1:4090445/147‑1 (MQ=255)
                                                                                                                   aaCTGTCGGCGATTTTCCATTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGAGCGCAATa                               <  2:4091603/150‑1 (MQ=255)
                                                                                                                         gggCGATTTTCCTGTATTTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCTT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                         <  1:3662335/149‑1 (MQ=255)
                                                                                                                           gctcTTTTCAATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCAAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                       <  1:4290005/147‑1 (MQ=255)
                                                                                                                           gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                       <  2:3946956/150‑1 (MQ=255)
                                                                                                                            cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                      <  2:4631979/150‑1 (MQ=255)
                                                                                                                             gcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                     <  2:747290/150‑1 (MQ=255)
                                                                                                                             gcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                     <  2:835881/150‑1 (MQ=255)
                                                                                                                               ttttCCAGTATGTTGCTAAAAATTTTATGTAAATAGGCCTGCTGGCCTT‑TTTTTTTTGTTT‑GGATTTTGAGCTTTTTGCCCAGCATTCAG‑ACGAAATTTGCCCGGTATTTGTGAAAAATTTCGCGACAGCGCGCAATAACCGTTCTCGag                   <  2:647069/150‑2 (MQ=255)
                                                                                                                               ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                   <  2:1868422/150‑1 (MQ=255)
                                                                                                                                   ccAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt               <  2:4952446/150‑1 (MQ=255)
                                                                                                                                          gtttatAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg        <  1:1937179/145‑1 (MQ=255)
                                                                                                                                          gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg        <  2:1815886/150‑1 (MQ=255)
                                                                                                                                           ttGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa       <  2:4868669/150‑1 (MQ=255)
                                                                                                                                            tGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAAGTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt      <  1:1078810/150‑1 (MQ=255)
                                                                                                                                            tGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt      <  2:2519498/150‑1 (MQ=255)
                                                                                                                                            tGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt      <  1:239558/150‑1 (MQ=255)
                                                                                                                                              cTAAAAATTTTTTGAAAAACGGCGGGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGATTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc    <  1:2263224/150‑1 (MQ=255)
                                                                                                                                                aaaaATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg  <  1:3085557/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG  >  CP009273/450143‑450436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑