Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 456,298 T→A 100% L652Q (CTG→CAG)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273456,2980TA99.7% 1020.8 / ‑4.8 308L652Q (CTG→CAG) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (157/150);  total (158/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 308 total aligned reads displayed.

GGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATG  >  CP009273/456151‑456444
                                                                                                                                                   |                                                                                                                                                  
ggACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAgg                                                                                                                                                  >  1:982308/1‑150 (MQ=255)
 gACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAggg                                                                                                                                                 <  1:1876045/150‑1 (MQ=255)
  aCGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAgggg                                                                                                                                                <  1:3923463/150‑1 (MQ=255)
  aCGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAgggg                                                                                                                                                <  1:4535850/150‑1 (MQ=255)
   cGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGa                                                                                                                                               <  2:1357251/150‑1 (MQ=255)
      aaGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCa                                                                                                                                            <  2:1636097/150‑1 (MQ=255)
      aaGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCa                                                                                                                                            <  1:4669512/150‑1 (MQ=255)
       aGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCaa                                                                                                                                           >  1:1583942/1‑150 (MQ=255)
       aGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCaa                                                                                                                                           <  1:4898401/150‑1 (MQ=255)
         tGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAAcc                                                                                                                                         <  1:2637173/150‑1 (MQ=255)
          gggCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAAccc                                                                                                                                        >  2:4725334/1‑150 (MQ=255)
            gCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTg                                                                                                                                      <  2:2360984/150‑1 (MQ=255)
              ggTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAt                                                                                                                                    <  2:4592730/150‑1 (MQ=255)
              ggTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAt                                                                                                                                    <  2:2106569/150‑1 (MQ=255)
                 gACTTGCTGACCATTGAAACAGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAtttt                                                                                                                                 <  1:609723/150‑1 (MQ=255)
                  aCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAttttt                                                                                                                                <  1:1012599/150‑1 (MQ=255)
                  aCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAttttt                                                                                                                                >  1:4123068/1‑150 (MQ=255)
                   cTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTa                                                                                                                               <  2:5045469/150‑1 (MQ=255)
                   cTTGCTGACCATTGAAACCGCATGTGTACCGGGTAAAGGCAAACTGACCTATACCGGTTCTCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTa                                                                                                                               >  1:2234611/1‑150 (MQ=255)
                    ttGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACACTGATTTTTAc                                                                                                                              <  2:2582597/150‑1 (MQ=255)
                         gACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGaaaa                                                                                                                         >  1:2164586/1‑150 (MQ=255)
                          aCCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCAATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGaaaaa                                                                                                                        >  1:1264300/1‑150 (MQ=255)
                           ccATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAAc                                                                                                                       >  1:2508507/1‑150 (MQ=255)
                              ttGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTg                                                                                                                    >  2:2943745/1‑150 (MQ=255)
                               tGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGa                                                                                                                   <  1:3113041/150‑1 (MQ=255)
                               ggAACCCGCTTGTGTTCCGGGTAAAGGCACACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGa                                                                                                                   <  2:943434/149‑1 (MQ=255)
                                gAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGAc                                                                                                                  <  2:1952395/150‑1 (MQ=255)
                                 aaaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACa                                                                                                                 >  1:1059506/1‑150 (MQ=255)
                                 aaaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACa                                                                                                                 <  2:845572/150‑1 (MQ=255)
                                 aaaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACa                                                                                                                 >  2:3738066/1‑150 (MQ=255)
                                     cGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca                                                                                                             <  1:1672066/150‑1 (MQ=255)
                                     cGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca                                                                                                             <  1:2317594/150‑1 (MQ=255)
                                              tCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATccacgtccacgt                                                                                                    >  1:2574975/1‑150 (MQ=255)
                                                 gggTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAcc                                                                                                 >  2:3541263/1‑150 (MQ=255)
                                                   gTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCgg                                                                                               >  2:75949/1‑150 (MQ=255)
                                                          cAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGc                                                                                        <  2:1162485/150‑1 (MQ=255)
                                                          cAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGc                                                                                        <  2:2599643/150‑1 (MQ=255)
                                                            aaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGa                                                                                      <  1:1964922/150‑1 (MQ=255)
                                                             aCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGAc                                                                                     >  2:1683706/1‑150 (MQ=255)
                                                              cTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACg                                                                                    >  2:1488114/1‑150 (MQ=255)
                                                              cTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACg                                                                                    >  1:3875461/1‑150 (MQ=255)
                                                               tGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGc                                                                                   >  2:3988630/1‑150 (MQ=255)
                                                                gACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGTGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGcc                                                                                  <  1:180277/150‑1 (MQ=255)
                                                                gACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGcc                                                                                  >  2:1109856/1‑150 (MQ=255)
                                                                 aCCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCg                                                                                 <  2:1841898/150‑1 (MQ=255)
                                                                 aCCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCg                                                                                 >  1:322340/1‑150 (MQ=255)
                                                                     ataCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAg                                                                             >  2:3218645/1‑150 (MQ=255)
                                                                     ataCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAg                                                                             >  2:341175/1‑150 (MQ=255)
                                                                     ataCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATACACGTCCACGTACCGGAAGGTGCGACGCCGAAAg                                                                             <  2:719872/150‑1 (MQ=255)
                                                                      taCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGa                                                                            <  2:3263690/150‑1 (MQ=255)
                                                                       aCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGAt                                                                           <  1:4053287/150‑1 (MQ=255)
                                                                        ccGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATg                                                                          <  2:5180956/150‑1 (MQ=255)
                                                                        ccGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATg                                                                          <  1:3450882/150‑1 (MQ=255)
                                                                         cGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATgg                                                                         <  1:4336370/150‑1 (MQ=255)
                                                                           gTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTc                                                                       <  2:2798532/150‑1 (MQ=255)
                                                                           gTTCGCTCGGCGAAGTGATGCAGGAGGCCATTCAGGCGGCGTTAACGGTGGGTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTc                                                                       <  2:3805036/150‑1 (MQ=255)
                                                                            ttCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTcc                                                                      <  2:4410101/150‑1 (MQ=255)
                                                                               gCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAg                                                                   >  1:4957090/1‑150 (MQ=255)
                                                                                cTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGt                                                                  <  1:6451/150‑1 (MQ=255)
                                                                                 tCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGTGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTg                                                                 <  2:1058674/150‑1 (MQ=255)
                                                                                   ggCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGcc                                                               >  1:3377231/1‑150 (MQ=255)
                                                                                    gCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCg                                                              <  1:5113740/150‑1 (MQ=255)
                                                                                      gAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGt                                                            >  2:4266621/1‑150 (MQ=255)
                                                                                        aGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTCCCGGACGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTAt                                                          >  2:3867614/1‑150 (MQ=255)
                                                                                        aGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTAt                                                          <  2:4177618/150‑1 (MQ=255)
                                                                                        aGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTAt                                                          >  1:4461131/1‑150 (MQ=255)
                                                                                          tGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTg                                                        <  2:533805/150‑1 (MQ=255)
                                                                                           gATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGc                                                       >  1:2266907/1‑150 (MQ=255)
                                                                                            aTGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTTCGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCt                                                      <  1:3001940/150‑1 (MQ=255)
                                                                                               cAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtg                                                   <  2:4978970/150‑1 (MQ=255)
                                                                                               cAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtg                                                   >  1:3460005/1‑150 (MQ=255)
                                                                                                aGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgt                                                  >  1:1290783/1‑150 (MQ=255)
                                                                                                  gAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGc                                                <  1:4203151/150‑1 (MQ=255)
                                                                                                   aGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCa                                               >  2:3299389/1‑150 (MQ=255)
                                                                                                   aGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCa                                               >  2:3308873/1‑150 (MQ=255)
                                                                                                    gTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCAc                                              >  1:3597360/1‑150 (MQ=255)
                                                                                                        aTTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACcgcg                                          >  1:4157768/1‑150 (MQ=255)
                                                                                                        aTTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACcgcg                                          >  2:3687746/1‑150 (MQ=255)
                                                                                                        aTTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACcgcg                                          >  1:763691/1‑150 (MQ=255)
                                                                                                            aGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTgg                                      >  1:776698/1‑150 (MQ=255)
                                                                                                             ggcggcGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGt                                     <  1:3342217/150‑1 (MQ=255)
                                                                                                                   gTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGACGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                               >  2:3044137/1‑150 (MQ=255)
                                                                                                                   gTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                               >  2:3644093/1‑150 (MQ=255)
                                                                                                                   gTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                               >  1:2144287/1‑150 (MQ=255)
                                                                                                                   gTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                               <  1:4324986/150‑1 (MQ=255)
                                                                                                                     tAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGcc                             <  2:4452989/150‑1 (MQ=255)
                                                                                                                         ggtggtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGAc                         >  2:1536080/1‑150 (MQ=255)
                                                                                                                              ttCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTa                    >  2:4829402/1‑150 (MQ=255)
                                                                                                                                cgtgcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAAc                  <  2:561831/150‑1 (MQ=255)
                                                                                                                                    cgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCgg              <  1:484531/150‑1 (MQ=255)
                                                                                                                                      cgtgcgGACACACAGGGGCTCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGtt            <  1:2032087/150‑1 (MQ=255)
                                                                                                                                        tgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCg          >  1:1889131/1‑150 (MQ=255)
                                                                                                                                          cgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTg        >  1:1198108/1‑150 (MQ=255)
                                                                                                                                            gAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGcc      >  2:2446743/1‑150 (MQ=255)
                                                                                                                                            gAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGcc      <  2:70241/150‑1 (MQ=255)
                                                                                                                                            gAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCGATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGcc      >  2:450328/1‑150 (MQ=255)
                                                                                                                                             aaaaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCg     >  1:4563986/1‑150 (MQ=255)
                                                                                                                                             aaaaaCAGGGGATCAACACTGATTTTTACGAAAATCGTGACATCCACGTCCACGTACAGGAAGGTGCGACGCAGAAAGATGTTCCGAGTGCCGGTAATGCTATGTGCACAGCGCTGGTTGCTGGCCTGACCGGTAACCCGGTTCGTGCCg     >  1:1846957/1‑150 (MQ=255)
                                                                                                                                               aaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGAt   >  2:1763753/1‑150 (MQ=255)
                                                                                                                                                aaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGAtg  >  1:2783932/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATG  >  CP009273/456151‑456444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑