Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 54,392 G→T 100% A104E (GCG→GAG)  surA ← peptidyl‑prolyl cis‑trans isomerase (PPIase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927354,3920GT100.0% 1076.2 / NA 320A104E (GCG→GAG) surApeptidyl‑prolyl cis‑trans isomerase (PPIase)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (162/158);  total (162/158)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 322 total aligned reads displayed.

AGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCGCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCC  >  CP009273/54245‑54538
                                                                                                                                                   |                                                                                                                                                  
aGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTctcg                                                                                                                                                  <  2:5234590/150‑1 (MQ=255)
aGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTctcg                                                                                                                                                  <  1:1790708/150‑1 (MQ=255)
 ggATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATACAGCGTCATGTTGTTCTGTTTCTCGa                                                                                                                                                 >  2:1489995/1‑150 (MQ=255)
    tGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGt                                                                                                                                              <  1:4970092/150‑1 (MQ=255)
    tGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGt                                                                                                                                              >  1:3128865/1‑150 (MQ=255)
    tGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGt                                                                                                                                              >  1:5059712/1‑150 (MQ=255)
    tGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGt                                                                                                                                              <  1:272289/150‑1 (MQ=255)
     ggTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGtt                                                                                                                                             <  1:860843/150‑1 (MQ=255)
      gTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTa                                                                                                                                            <  2:552184/150‑1 (MQ=255)
      gTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTa                                                                                                                                            <  2:1135850/150‑1 (MQ=255)
       tGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAg                                                                                                                                           >  2:4940028/1‑150 (MQ=255)
        gATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGc                                                                                                                                          >  1:4654130/1‑150 (MQ=255)
        gATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGc                                                                                                                                          <  1:1898017/150‑1 (MQ=255)
        gATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGc                                                                                                                                          >  1:2241414/1‑150 (MQ=255)
         aTGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCa                                                                                                                                         <  2:4189691/150‑1 (MQ=255)
         aTGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCa                                                                                                                                         <  2:2487130/150‑1 (MQ=255)
            cgacgaCGCACCTAGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATc                                                                                                                                      <  2:4540421/150‑1 (MQ=255)
             gacgacGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCg                                                                                                                                     >  2:3727967/1‑150 (MQ=255)
             gacgacGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCg                                                                                                                                     >  2:1095516/1‑150 (MQ=255)
                  cgCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCATTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGATTCTCGATGTTAGCAATCGCCTGa                                                                                                                                >  2:1232889/1‑150 (MQ=255)
                   gCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGAt                                                                                                                               <  2:5000787/150‑1 (MQ=255)
                     aCCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATcc                                                                                                                             <  2:3188769/150‑1 (MQ=255)
                          gttgttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAgctg                                                                                                                        <  2:720244/150‑1 (MQ=255)
                            tgttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAgctgct                                                                                                                      >  2:2156883/1‑150 (MQ=255)
                            tgttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAgctgct                                                                                                                      <  2:1816518/150‑1 (MQ=255)
                             gttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTc                                                                                                                     <  1:445452/150‑1 (MQ=255)
                              ttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCa                                                                                                                    >  2:2050337/1‑150 (MQ=255)
                              ttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCa                                                                                                                    >  1:15928/1‑150 (MQ=255)
                                tcGCACTTCAGAGATATTCATCTCTTTGCGGATCTGGTTACGTTAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATc                                                                                                                  <  1:4614388/149‑1 (MQ=255)
                                   cACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGga                                                                                                               >  2:1853164/1‑150 (MQ=255)
                                   cACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGga                                                                                                               <  1:3143257/150‑1 (MQ=255)
                                     cTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgaga                                                                                                             <  2:1254204/150‑1 (MQ=255)
                                      ttCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAt                                                                                                            >  2:359589/1‑150 (MQ=255)
                                      ttCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAt                                                                                                            <  1:4136898/150‑1 (MQ=255)
                                       tCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAtt                                                                                                           <  1:1878212/150‑1 (MQ=255)
                                           agaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCa                                                                                                       >  1:1164676/1‑150 (MQ=255)
                                           agaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGGTGGTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCa                                                                                                       >  2:4724980/1‑150 (MQ=255)
                                            gaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCAc                                                                                                      >  1:2419628/1‑150 (MQ=255)
                                            gaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCAc                                                                                                      <  1:3802019/150‑1 (MQ=255)
                                             aTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACt                                                                                                     <  2:4624664/150‑1 (MQ=255)
                                             aTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACt                                                                                                     >  1:1945016/1‑150 (MQ=255)
                                               aaTCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTcc                                                                                                   <  1:732627/150‑1 (MQ=255)
                                                  catcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAt                                                                                                >  1:3535051/1‑150 (MQ=255)
                                                  catcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAt                                                                                                <  2:1625476/150‑1 (MQ=255)
                                                   atcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAtt                                                                                               >  1:3390752/1‑150 (MQ=255)
                                                     ctctTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAtttt                                                                                             >  2:2651343/1‑150 (MQ=255)
                                                      tctTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTc                                                                                            <  1:2937973/150‑1 (MQ=255)
                                                       ctTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATAGCCTGATACAGATGCTCATCGGAGATTTTCAATACAATTTTCt                                                                                           >  2:333630/1‑150 (MQ=255)
                                                       ctTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGAGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTTCTCATCGGAGATTTTCACTCCCAttttat                                                                                           <  2:1997351/150‑3 (MQ=255)
                                                        tttGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTg                                                                                          >  1:4385498/1‑150 (MQ=255)
                                                        cttGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTg                                                                                          >  2:2413904/2‑150 (MQ=255)
                                                               aTCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATc                                                                                   >  2:4395223/1‑150 (MQ=255)
                                                                tCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCt                                                                                  >  1:2825649/1‑150 (MQ=255)
                                                                 cTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTg                                                                                 <  1:5192900/150‑1 (MQ=255)
                                                                  tGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAATTTCTGCCCCATCTGc                                                                                >  2:3298958/1‑150 (MQ=255)
                                                                    gTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAg                                                                              <  2:4848506/150‑1 (MQ=255)
                                                                      tACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGga                                                                            <  1:1503663/150‑1 (MQ=255)
                                                                       aCGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgat                                                                           <  2:2837707/150‑1 (MQ=255)
                                                                       aCGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgat                                                                           <  1:3851150/150‑1 (MQ=255)
                                                                        cGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatg                                                                          <  1:5308401/150‑1 (MQ=255)
                                                                          aTAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatgat                                                                        >  2:3958484/1‑150 (MQ=255)
                                                                                gttgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATc                                                                  >  2:1911269/1‑150 (MQ=255)
                                                                                  tgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                                <  1:560479/150‑1 (MQ=255)
                                                                                  tgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                                <  1:3927340/150‑1 (MQ=255)
                                                                                     aGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGa                                                             >  1:4240965/1‑150 (MQ=255)
                                                                                      gTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGAt                                                            >  1:3290586/1‑150 (MQ=255)
                                                                                         cAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCaa                                                         <  2:103842/150‑1 (MQ=255)
                                                                                          aGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCAACGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCaaa                                                        >  1:2127003/1‑150 (MQ=255)
                                                                                            tCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACg                                                      <  1:4046679/150‑1 (MQ=255)
                                                                                               aTCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTc                                                   >  1:2483928/1‑150 (MQ=255)
                                                                                                tCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTcc                                                  <  1:424938/150‑1 (MQ=255)
                                                                                                 cGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCa                                                 <  2:368405/150‑1 (MQ=255)
                                                                                                  gTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCAt                                                <  2:865975/150‑1 (MQ=255)
                                                                                                  gTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCAt                                                <  2:233244/150‑1 (MQ=255)
                                                                                                   tAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATg                                               <  1:2567656/150‑1 (MQ=255)
                                                                                                     aGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGAt                                             >  1:1374449/1‑150 (MQ=255)
                                                                                                      gCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGAtt                                            <  1:362818/150‑1 (MQ=255)
                                                                                                           aCGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCACTCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtggtg                                       <  2:1382303/150‑1 (MQ=255)
                                                                                                             ggCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGgc                                     <  2:277780/150‑1 (MQ=255)
                                                                                                                 gcgcATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGCTGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAg                                 <  2:4280536/150‑1 (MQ=255)
                                                                                                                  cgcATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgc                                >  2:3848854/1‑150 (MQ=255)
                                                                                                                   gcATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcg                               >  1:1051127/1‑150 (MQ=255)
                                                                                                                    cATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTACACTCCCATTTTCTGCCCCAACTGCAGGATGATTTGATACATGATCAAACGTTCCATGATTTTGTGGCGCAgcgt                              >  1:3714785/1‑150 (MQ=255)
                                                                                                                     aTCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtc                             >  2:3078562/1‑150 (MQ=255)
                                                                                                                     aTCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtc                             <  1:516916/150‑1 (MQ=255)
                                                                                                                         gATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtcgcgt                         >  1:3460858/1‑150 (MQ=255)
                                                                                                                         gATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtcgcgt                         <  2:430196/150‑1 (MQ=255)
                                                                                                                           tCCAGCGTCTTGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCTGAGATTTTCACTCCAATTATCTGCCCCATCTGCAGGATGATTTGAACCATGATAAAACGTTACATGATTTGGATGCGCAGCGTCGAGtca                       >  2:845759/1‑150 (MQ=255)
                                                                                                                           tCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGtca                       <  1:45560/150‑1 (MQ=255)
                                                                                                                           tCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGtca                       >  1:941575/1‑150 (MQ=255)
                                                                                                                              aGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGtcatca                    <  2:4084118/150‑1 (MQ=255)
                                                                                                                                 gTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCCTCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGa                 <  1:1759240/150‑1 (MQ=255)
                                                                                                                                  tCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGaa                >  1:3211848/1‑150 (MQ=255)
                                                                                                                                   cATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAg               <  2:2275463/150‑1 (MQ=255)
                                                                                                                                       ttgttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTtgc           >  2:5072781/1‑150 (MQ=255)
                                                                                                                                            cTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcct      >  1:810720/1‑150 (MQ=255)
                                                                                                                                            cTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCAATGATCAAACGTTCCAAGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcct      >  1:635730/1‑150 (MQ=255)
                                                                                                                                             tGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcctt     <  2:1118071/150‑1 (MQ=255)
                                                                                                                                              gTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgccttg    <  1:75915/150‑1 (MQ=255)
                                                                                                                                               tttCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgccttgc   >  1:2890963/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCGCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCC  >  CP009273/54245‑54538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑