Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 496,210 G→T 26.9% M210I (ATG→ATT)  gsk → inosine/guanosine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273496,2100GT26.9% 711.6 / 210.5 333M210I (ATG→ATT) gskinosine/guanosine kinase
Reads supporting (aligned to +/- strand):  ref base G (128/115);  new base T (36/54);  total (164/169)
Fisher's exact test for biased strand distribution p-value = 4.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 335 total aligned reads displayed.

GAACGTACCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGATGAAGCCGAAGC  >  CP009273/496061‑496359
                                                                                                                                                     |                                                                                                                                                     
  aCGTACCTTTGCTACCAGTCCAGGCCACATGAACCAGCTGCGTGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGaa                                                                                                                                                     <  2:3359752/150‑1 (MQ=255)
   cGTACCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGaaa                                                                                                                                                    >  1:2275592/1‑150 (MQ=255)
    gTACCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGACAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAg                                                                                                                                                   <  1:3992098/150‑1 (MQ=255)
    gTACCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAg                                                                                                                                                   <  2:3859572/150‑1 (MQ=255)
    gTACCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAg                                                                                                                                                   >  2:5005290/1‑150 (MQ=255)
     tACCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGc                                                                                                                                                  >  1:1012022/1‑150 (MQ=255)
     tACCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGc                                                                                                                                                  >  2:2126626/1‑150 (MQ=255)
      aCCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGcc                                                                                                                                                 >  1:2642553/1‑150 (MQ=255)
          ttGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTg                                                                                                                                             <  1:5180585/150‑1 (MQ=255)
            gCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAg                                                                                                                                           <  2:1380482/150‑1 (MQ=255)
             cTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGt                                                                                                                                          <  2:4912041/150‑1 (MQ=255)
             cTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGt                                                                                                                                          >  2:4609998/1‑150 (MQ=255)
               aTCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTAc                                                                                                                                        >  2:3192616/1‑150 (MQ=255)
               aTCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTAc                                                                                                                                        >  1:2173774/1‑150 (MQ=255)
                  aGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTAcgcg                                                                                                                                     >  2:426942/1‑150 (MQ=255)
                  aGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTAcgcg                                                                                                                                     <  1:811030/150‑1 (MQ=255)
                   gTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCga                                                                                                                                    <  2:1953618/150‑1 (MQ=255)
                    tCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCgaa                                                                                                                                   <  1:136247/150‑1 (MQ=255)
                      cAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCgaaga                                                                                                                                 <  2:4830344/150‑1 (MQ=255)
                      cAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCgaaga                                                                                                                                 >  1:2566353/1‑150 (MQ=255)
                       aGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCgaagaa                                                                                                                                <  2:742554/150‑1 (MQ=255)
                       aGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCgaagaa                                                                                                                                >  1:4401805/1‑150 (MQ=255)
                       aGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCgaagaa                                                                                                                                >  1:4001992/1‑150 (MQ=255)
                          ccACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAata                                                                                                                             <  2:4493093/150‑1 (MQ=255)
                            acaTGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTCCGCGAAGAAAtata                                                                                                                           >  2:3975262/1‑150 (MQ=255)
                                  aCCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTAc                                                                                                                     >  1:4588956/1‑150 (MQ=255)
                                     aGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCgg                                                                                                                  <  2:2349805/150‑1 (MQ=255)
                                      gCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCggt                                                                                                                 <  2:4006052/150‑1 (MQ=255)
                                        tGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCggtgg                                                                                                               >  1:1568171/1‑150 (MQ=255)
                                          cGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGgtggtg                                                                                                             <  2:1216711/150‑1 (MQ=255)
                                             gCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTg                                                                                                          >  2:2859396/1‑150 (MQ=255)
                                             gCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTg                                                                                                          <  2:4303766/150‑1 (MQ=255)
                                               tGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGAc                                                                                                        <  1:2048945/150‑1 (MQ=255)
                                                 gaaGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGc                                                                                                      >  2:1270954/2‑150 (MQ=255)
                                                  aaGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATCTCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCt                                                                                                     <  2:4098892/150‑1 (MQ=255)
                                                     cATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTggg                                                                                                  >  1:404092/1‑150 (MQ=255)
                                                     cATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTggg                                                                                                  <  1:4247312/150‑1 (MQ=255)
                                                      aTTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGc                                                                                                 <  2:2066175/150‑1 (MQ=255)
                                                      aTTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGc                                                                                                 >  1:1793332/1‑150 (MQ=255)
                                                          cGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCa                                                                                             <  1:61122/150‑1 (MQ=255)
                                                           ggAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCaa                                                                                            >  1:3423959/1‑150 (MQ=255)
                                                             aaGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGt                                                                                          >  2:114005/1‑150 (MQ=255)
                                                              aGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGtt                                                                                         >  2:90968/1‑150 (MQ=255)
                                                              aGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGtt                                                                                         <  1:4129859/150‑1 (MQ=255)
                                                              aGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGtt                                                                                         <  1:1466886/150‑1 (MQ=255)
                                                                  gtgATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTc                                                                                     >  2:3023240/1‑150 (MQ=255)
                                                                   tgATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCa                                                                                    <  1:3188035/150‑1 (MQ=255)
                                                                     aTTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCAtt                                                                                  <  1:2532144/150‑1 (MQ=255)
                                                                     aTTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCAtt                                                                                  <  1:1909539/150‑1 (MQ=255)
                                                                      ttGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTg                                                                                 <  2:3733293/150‑1 (MQ=255)
                                                                      ttGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTg                                                                                 >  2:1679748/1‑150 (MQ=255)
                                                                            gAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCgaga                                                                           <  2:4414668/150‑1 (MQ=255)
                                                                              gCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAAt                                                                         <  1:3907794/150‑1 (MQ=255)
                                                                              gCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAAt                                                                         >  1:1281660/1‑150 (MQ=255)
                                                                                cTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATcc                                                                       >  1:4708007/1‑150 (MQ=255)
                                                                                cTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGACTcc                                                                       >  2:3236554/1‑150 (MQ=255)
                                                                                cTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATcc                                                                       <  1:3144632/150‑1 (MQ=255)
                                                                                   ggCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCa                                                                    >  1:3729645/1‑150 (MQ=255)
                                                                                    gCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAg                                                                   >  1:2246409/1‑150 (MQ=255)
                                                                                    gCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAg                                                                   >  1:187972/1‑150 (MQ=255)
                                                                                      aCTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAgtg                                                                 <  1:130254/150‑1 (MQ=255)
                                                                                        tGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAgtggt                                                               >  2:5229080/1‑150 (MQ=255)
                                                                                        tGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAgtggt                                                               >  2:3274400/1‑150 (MQ=255)
                                                                                         ggTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAgtggtg                                                              <  2:1775956/150‑1 (MQ=255)
                                                                                         ggTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAgtggtg                                                              <  1:2310418/150‑1 (MQ=255)
                                                                                              tcACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGgcagc                                                         <  2:3644468/150‑1 (MQ=255)
                                                                                                 ccTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCACCCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAAt                                                      >  2:4654314/1‑150 (MQ=255)
                                                                                                  cTCATCTCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCTTGATAGCCATTGAGTTCGCGAAGAAATAGAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAAtt                                                     <  2:1550402/150‑1 (MQ=255)
                                                                                                   tCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTc                                                    >  2:5140367/1‑150 (MQ=255)
                                                                                                     atatCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCt                                                  >  1:479652/1‑150 (MQ=255)
                                                                                                            gTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGAt                                           <  2:1396771/150‑1 (MQ=255)
                                                                                                             tGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATc                                          <  1:1640516/150‑1 (MQ=255)
                                                                                                             tGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATc                                          <  2:4537377/150‑1 (MQ=255)
                                                                                                             tGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATc                                          <  2:3780254/150‑1 (MQ=255)
                                                                                                               cGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCAc                                        >  2:4211859/1‑150 (MQ=255)
                                                                                                                     aaGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTctct                                  <  2:3265679/150‑1 (MQ=255)
                                                                                                                      aGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTa                                 <  1:5055921/150‑1 (MQ=255)
                                                                                                                       gCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTAt                                <  2:88125/150‑1 (MQ=255)
                                                                                                                           ggTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCtt                            >  2:4596511/1‑150 (MQ=255)
                                                                                                                            gTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTg                           >  2:2115543/1‑150 (MQ=255)
                                                                                                                              gAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCg                         >  1:3785485/1‑150 (MQ=255)
                                                                                                                              gAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCg                         >  1:1728823/1‑150 (MQ=255)
                                                                                                                                aCCCATGCCGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGAt                       <  1:3253194/150‑1 (MQ=255)
                                                                                                                                aCCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGAt                       >  2:2618145/1‑150 (MQ=255)
                                                                                                                                  ccATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGa                     >  1:2957974/1‑150 (MQ=255)
                                                                                                                                    aTGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAAc                   <  1:2139032/150‑1 (MQ=255)
                                                                                                                                       ccGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGaa                >  2:275285/1‑150 (MQ=255)
                                                                                                                                       ccGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGaa                <  1:5003460/150‑1 (MQ=255)
                                                                                                                                        cGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAg               <  2:1012048/150‑1 (MQ=255)
                                                                                                                                        cGGAAGCAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAg               <  2:3339960/150‑1 (MQ=255)
                                                                                                                                          gAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGAt             <  1:978967/150‑1 (MQ=255)
                                                                                                                                          gAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGAt             >  1:329565/1‑150 (MQ=255)
                                                                                                                                              cAACCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCTATGAACGAAGATGAAg         >  2:174084/1‑150 (MQ=255)
                                                                                                                                                aCCATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGATGAAGcc       <  2:618551/150‑1 (MQ=255)
                                                                                                                                                 ccATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGATGAAGCCg      >  1:1043614/1‑150 (MQ=255)
                                                                                                                                                  cATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGATGAAGCCGa     >  1:286849/1‑150 (MQ=255)
                                                                                                                                                  cATTAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGATGAAGCCGa     >  1:2715274/1‑150 (MQ=255)
                                                                                                                                                  cATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACTTCTCTATCCTTGCGATGAACGAAGATGAAGCCGa     >  1:94342/1‑150 (MQ=255)
                                                                                                                                                    tGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGATGAAGCCGAAg   <  1:3642953/150‑1 (MQ=255)
                                                                                                                                                     gAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGATGAAGCCGAAGc  >  1:4657702/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAACGTACCTTTGCTATCAGTCCAGGCCACATGAACCAGCTGCGGGCTGAAAGCATTCCGGAAGATGTGATTGCCGGAGCCTCGGCACTGGTTCTCACCTCATATCTGGTGCGTTGCAAGCCGGGTGAACCCATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAAGATCACGTCTCTATCCTTGCGATGAACGAAGATGAAGCCGAAGC  >  CP009273/496061‑496359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑