Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 518,243 G→T 12.4% R791L (CGA→CTA)  ybbP → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273518,2430GT12.4% 1054.5 / 69.6 336R791L (CGA→CTA) ybbPputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (117/175);  new base T (17/27);  total (134/202)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 348 total aligned reads displayed.

GGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTC  >  CP009273/518094‑518392
                                                                                                                                                     |                                                                                                                                                     
ggtggCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGcgcg                                                                                                                                                       >  2:2510630/1‑150 (MQ=255)
      cGCAATGGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGt                                                                                                                                                 >  2:2530830/1‑150 (MQ=255)
       gCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGtt                                                                                                                                                <  2:3469608/150‑1 (MQ=255)
       gCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGtt                                                                                                                                                <  1:2049686/150‑1 (MQ=255)
       gCAAGTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGtt                                                                                                                                                <  2:2977856/150‑1 (MQ=255)
         aaTTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTAGGCGGCTGGCTGGGTGCGCGACTGGTTaa                                                                                                                                              >  1:1523734/1‑150 (MQ=255)
          aTTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAg                                                                                                                                             <  1:2886210/150‑1 (MQ=255)
            tGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACGGGTTAAggg                                                                                                                                           >  1:245717/1‑150 (MQ=255)
              gTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTa                                                                                                                                         <  2:5019575/150‑1 (MQ=255)
               tGCGGAAACGGCGCTGTCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGTATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGAGGCTGGCTGGGTGAGCGACTGGTTAAGGGTaa                                                                                                                                        <  1:3795988/150‑1 (MQ=255)
                  ggAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTAGGCGGCTGGGTGGGTGCGCGACGGGTTAAGGGTAAGgc                                                                                                                                     >  1:2333929/1‑150 (MQ=255)
                    aaaCGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCTATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGgcgc                                                                                                                                   >  2:2456558/1‑150 (MQ=255)
                     aaCGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCt                                                                                                                                  >  2:1603806/1‑150 (MQ=255)
                          cgcTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCa                                                                                                                             <  1:2544702/150‑1 (MQ=255)
                          cgcTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCa                                                                                                                             <  1:3141454/150‑1 (MQ=255)
                           gcTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAg                                                                                                                            <  1:4113332/150‑1 (MQ=255)
                           gcTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAg                                                                                                                            >  1:3031561/1‑150 (MQ=255)
                             tGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGc                                                                                                                          >  1:2686361/1‑150 (MQ=255)
                             tGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGTCTGGGTAAGGGTAAGGCGCTGTTCAGGc                                                                                                                          >  2:4544831/1‑150 (MQ=255)
                             tGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGc                                                                                                                          <  2:2658419/150‑1 (MQ=255)
                             tGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTAGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGc                                                                                                                          >  1:1941472/1‑150 (MQ=255)
                              ggCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACGGGGTAAGGGTAAGGCGCTGTTCAGGCa                                                                                                                         >  1:4262258/1‑150 (MQ=255)
                                 aGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGtt                                                                                                                      <  2:1300300/150‑1 (MQ=255)
                                 aGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGtt                                                                                                                      >  2:4596325/1‑150 (MQ=255)
                                    gtTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGc                                                                                                                   >  2:198909/1‑150 (MQ=255)
                                       gCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCGTTTCGGCGGCTGGCTGGGTGCGCGACTGGGTAAGGGTAAGGCGCAGTTAAGGCAGGTTGCggg                                                                                                                >  1:4991510/1‑150 (MQ=255)
                                          ggCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACGGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGtg                                                                                                             >  1:2099936/1‑150 (MQ=255)
                                            cGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGtgat                                                                                                           <  1:4539707/150‑1 (MQ=255)
                                            cGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTAGGCGGCTGGCTGGGTGCGCGACAGGTTAAGGGTAACTCGCTGTTCATGCAGTTTTCTGGGtgat                                                                                                           >  1:1094433/1‑150 (MQ=255)
                                              aaaGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCCGCGGAGCGCTGCTGCTGTCGCGTTTCGGCGGCTGGCTGGGTGCGCGACGGGGTAAGGGTAAGGCGCTGTTCAGGGAGTTTGCGGGGtgatga                                                                                                         >  1:3747820/1‑150 (MQ=255)
                                              aaaGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGGTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGtgatga                                                                                                         >  2:4055051/1‑150 (MQ=255)
                                              aaaGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACGGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTTCGGGGtgttga                                                                                                         >  1:3215281/1‑150 (MQ=255)
                                               aaGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATgag                                                                                                        <  2:1109148/150‑1 (MQ=255)
                                               aaGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATgag                                                                                                        >  2:1325792/1‑150 (MQ=255)
                                                aGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATgaga                                                                                                       <  2:4725391/150‑1 (MQ=255)
                                                aGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATgaga                                                                                                       <  2:715532/150‑1 (MQ=255)
                                                aGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTAAGGCAGTTTGCGGGGTGATgaga                                                                                                       >  2:4092624/1‑150 (MQ=255)
                                                 gtgtTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTTCGGGGTGATGagag                                                                                                      >  1:4611629/1‑150 (MQ=255)
                                                  tgtTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGt                                                                                                     <  1:3382216/150‑1 (MQ=255)
                                                        aTTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATaa                                                                                               <  1:2181245/150‑1 (MQ=255)
                                                        aTTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGGTTGCGGGGTGATGAGAGTAAATaa                                                                                               >  2:5304764/1‑150 (MQ=255)
                                                         ttttCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAg                                                                                              <  2:3107266/150‑1 (MQ=255)
                                                         ttttCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAg                                                                                              <  2:1817458/150‑1 (MQ=255)
                                                            tCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGGTGGGTGCGCGACTGGTTAAGGGTAATGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAgggg                                                                                           >  2:79930/1‑150 (MQ=255)
                                                            tCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAgggg                                                                                           <  2:1538391/150‑1 (MQ=255)
                                                             ccGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCGGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAATGGGc                                                                                          >  1:1621459/1‑150 (MQ=255)
                                                               gTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCGGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGt                                                                                        >  2:2232467/1‑150 (MQ=255)
                                                                  ggAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCt                                                                                     <  2:1749980/150‑1 (MQ=255)
                                                                    aGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAATGGTAAGGCGCGGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTgg                                                                                   >  2:4019821/1‑150 (MQ=255)
                                                                     gCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGNGGCGTTCTGGt                                                                                  <  1:2761638/150‑1 (MQ=255)
                                                                     gCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGt                                                                                  <  1:2521771/150‑1 (MQ=255)
                                                                      ccAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGtt                                                                                 <  2:1660744/150‑1 (MQ=255)
                                                                       cAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTAATCCGCTTGCTGGGTGCGCGACATGTTAATGGTAAAGCGCAGATAAAGCAGTTTGCGGGGTGATGTGAGTAAATAAAGGGCGTTATGGttt                                                                                >  1:535627/1‑149 (MQ=255)
                                                                        aGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGa                                                                               <  2:567361/150‑1 (MQ=255)
                                                                         gACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGaa                                                                              <  2:2203806/150‑1 (MQ=255)
                                                                          aCTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAAt                                                                             <  1:1762948/150‑1 (MQ=255)
                                                                          aCTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAAt                                                                             <  1:3551508/150‑1 (MQ=255)
                                                                            tGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATgg                                                                           <  1:4211795/150‑1 (MQ=255)
                                                                            tGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATgg                                                                           <  2:5153739/150‑1 (MQ=255)
                                                                              gCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTTAATAAGGGGCGTTCTGGTTGAATGGaa                                                                         <  2:763874/150‑1 (MQ=255)
                                                                              gCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGaa                                                                         <  2:3893799/150‑1 (MQ=255)
                                                                                 aTTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTTCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGc                                                                      >  2:277086/1‑150 (MQ=255)
                                                                                    gtgGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGTACGCCtt                                                                   <  1:4225180/150‑1 (MQ=255)
                                                                                    gtgGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCtt                                                                   <  2:483131/150‑1 (MQ=255)
                                                                                    gtgGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCtt                                                                   <  2:4744047/150‑1 (MQ=255)
                                                                                    gtgGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCGTTTCGGCGGCTGGCTGGGTGGGCTACTGGTGAAGGGTAAGGCGCTGTTCAGGGAGTTTGGGGGGTGATGAGTGTAAATAAGGGGCGGGGTGGTTGTATGGTACGccgt                                                                   >  1:2424256/1‑148 (MQ=255)
                                                                                      ggATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGt                                                                 >  1:4415899/1‑150 (MQ=255)
                                                                                       gATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGtt                                                                <  2:1473164/150‑1 (MQ=255)
                                                                                       gATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGtt                                                                <  2:4130834/150‑1 (MQ=255)
                                                                                        aTTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCGTTTCGGCGGCTGGCTGGGTGCCCGACGGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGttt                                                               >  2:3666954/1‑150 (MQ=255)
                                                                                           gTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGGCTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTaaa                                                            >  2:1365192/1‑150 (MQ=255)
                                                                                           gTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTaaa                                                            >  2:4194463/1‑150 (MQ=255)
                                                                                           gTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCGGGGTGGGTGCGCGACGGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCGGGTTGTCTGGAACGCCGTGTTTtaa                                                            >  2:5249004/1‑150 (MQ=255)
                                                                                              cTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTTAGGGTAAGGCGCTGTTCAGGCAGGTTGCGGGGTGTTGAGATTAAATAAGGGGCGTTGTGGTTGAAAGGAACGCCTTGTTTAaatta                                                         >  1:4335783/1‑150 (MQ=255)
                                                                                              cTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAaatta                                                         <  2:4410784/150‑1 (MQ=255)
                                                                                                       aGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCCGTTTGGGGGGTGTTGAGAGTAAATAAGGGGCGTTCGGGGTGTATGGAACGCCTTGTTTAAATTAATTCTACaa                                                >  2:2406828/1‑150 (MQ=255)
                                                                                                          ggAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAatat                                             <  2:2632992/150‑1 (MQ=255)
                                                                                                          ggAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAatat                                             <  1:4875149/150‑1 (MQ=255)
                                                                                                           gAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATg                                            <  1:5233222/150‑1 (MQ=255)
                                                                                                            aGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGt                                           <  1:448909/150‑1 (MQ=255)
                                                                                                             gcgcTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGtt                                          <  2:3241591/150‑1 (MQ=255)
                                                                                                                 tgctgctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCAt                                      <  1:1010402/150‑1 (MQ=255)
                                                                                                                 tgctgctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCAt                                      <  2:86222/150‑1 (MQ=255)
                                                                                                                 tgctgctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCAt                                      <  2:1981999/150‑1 (MQ=255)
                                                                                                                     gctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATtgat                                  <  2:2134653/150‑1 (MQ=255)
                                                                                                                      ctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATtgatg                                 <  1:383733/150‑1 (MQ=255)
                                                                                                                       tgTCGCTTTTCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATtgatga                                <  1:3657841/150‑1 (MQ=255)
                                                                                                                       tgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATtgatga                                >  2:3421838/1‑150 (MQ=255)
                                                                                                                           gCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGtt                            <  2:3551935/150‑1 (MQ=255)
                                                                                                                              tttCGGCGGCTGGCTGGGTGCGCTACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTAtt                         <  1:1455217/150‑1 (MQ=255)
                                                                                                                                    cggcTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTg                   <  1:1325792/150‑1 (MQ=255)
                                                                                                                                      gctggctgGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGt                 <  1:949817/150‑1 (MQ=255)
                                                                                                                                         ggctggGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGAt              <  1:3636304/150‑1 (MQ=255)
                                                                                                                                           ctggGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGAttt            <  1:4725924/150‑1 (MQ=255)
                                                                                                                                           ctggGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGAttt            <  1:4063960/150‑1 (MQ=255)
                                                                                                                                                tGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTAt       <  2:2043892/150‑1 (MQ=255)
                                                                                                                                                tGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTAt       <  1:747844/150‑1 (MQ=255)
                                                                                                                                                tGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTAt       <  1:5133919/150‑1 (MQ=255)
                                                                                                                                                  cgcgACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCt     >  1:1702908/1‑150 (MQ=255)
                                                                                                                                                    cgACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCttt   >  2:1240008/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTC  >  CP009273/518094‑518392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑