Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 518,820 C→A 100% A35E (GCG→GAG)  rhsD → Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273518,8200CA99.3% 878.5 / ‑1.9 268A35E (GCG→GAG) rhsDRhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (132/134);  total (134/134)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 281 total aligned reads displayed.

TTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACG  >  CP009273/518673‑518965
                                                                                                                                                   |                                                                                                                                                 
ttgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCggcgtggagt                                                                                                                                                 >  2:686460/1‑150 (MQ=255)
ttgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCggcgtggagt                                                                                                                                                 >  2:1275488/1‑150 (MQ=255)
ttgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCggcgtggagt                                                                                                                                                 >  2:20773/1‑150 (MQ=255)
 tgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGgcgtggagtg                                                                                                                                                >  1:4656626/1‑150 (MQ=255)
 tgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGgcgtggagtg                                                                                                                                                <  2:1435591/150‑1 (MQ=255)
   cTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCt                                                                                                                                              >  1:4697528/1‑150 (MQ=255)
   cTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCt                                                                                                                                              >  1:706760/1‑150 (MQ=255)
   cTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCt                                                                                                                                              >  2:1877572/1‑150 (MQ=255)
   cTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCt                                                                                                                                              <  1:3152993/150‑1 (MQ=255)
    tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTc                                                                                                                                             >  1:2140571/1‑150 (MQ=255)
      acacAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCgg                                                                                                                                           >  1:541131/1‑150 (MQ=255)
         caGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgt                                                                                                                                        >  2:117054/1‑150 (MQ=255)
         caGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgt                                                                                                                                        >  2:5088706/1‑150 (MQ=255)
          aGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgtg                                                                                                                                       >  1:1357453/1‑150 (MQ=255)
          aGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgtg                                                                                                                                       >  2:2519119/1‑150 (MQ=255)
             gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTTCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTcc                                                                                                                                    <  2:926380/150‑1 (MQ=255)
             gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTcc                                                                                                                                    <  1:2514350/150‑1 (MQ=255)
             gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTcc                                                                                                                                    <  1:4170040/150‑1 (MQ=255)
              atatGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcg                                                                                                                                   <  2:807360/150‑1 (MQ=255)
                       aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATg                                                                                                                          <  2:3292586/150‑1 (MQ=255)
                          aCTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACt                                                                                                                       <  1:5066086/150‑1 (MQ=255)
                           cTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACtt                                                                                                                      >  2:2680400/1‑150 (MQ=255)
                              aaaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCgg                                                                                                                   >  2:843600/1‑150 (MQ=255)
                               aaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAACTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCggg                                                                                                                  >  2:748432/1‑150 (MQ=255)
                                aGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGc                                                                                                                 >  2:2953599/1‑150 (MQ=255)
                                aGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGc                                                                                                                 >  1:1690474/1‑150 (MQ=255)
                                 gggCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCa                                                                                                                <  1:3382475/150‑1 (MQ=255)
                                 gggCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCa                                                                                                                <  2:617849/150‑1 (MQ=255)
                                    cACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAAcc                                                                                                             >  2:3402961/1‑150 (MQ=255)
                                        ttATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGt                                                                                                         <  1:2273806/150‑1 (MQ=255)
                                         tataTGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTa                                                                                                        >  2:1489475/1‑150 (MQ=255)
                                           tatGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaaa                                                                                                      >  1:3781136/1‑150 (MQ=255)
                                             tGAGCGGAAAACCAGCGGCGCGTCAGGGAGATCTGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATc                                                                                                    <  2:4411669/150‑1 (MQ=255)
                                              gAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATcc                                                                                                   <  1:852622/150‑1 (MQ=255)
                                                 cGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgct                                                                                                <  2:4125598/150‑1 (MQ=255)
                                                 cGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATGGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgct                                                                                                <  2:4297607/150‑1 (MQ=255)
                                                  ggAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCA‑TGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctgc                                                                                              >  2:3444096/1‑150 (MQ=255)
                                                   gAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctgc                                                                                              <  1:162302/150‑1 (MQ=255)
                                                    caaaCCAGCGGCGCGTCAGGGAGATATGCCTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctgct                                                                                             <  1:3572787/149‑1 (MQ=255)
                                                    aaaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctgct                                                                                             <  2:3594554/150‑1 (MQ=255)
                                                    aaaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctgct                                                                                             <  2:971136/150‑1 (MQ=255)
                                                     aaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTTCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGctgctg                                                                                            >  2:498781/1‑150 (MQ=255)
                                                     aaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGctgctg                                                                                            >  1:2931560/1‑150 (MQ=255)
                                                     aaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGctgctg                                                                                            >  2:2351316/1‑150 (MQ=255)
                                                     aaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGctgctg                                                                                            <  2:1379729/150‑1 (MQ=255)
                                                      aaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgg                                                                                           >  2:2297580/1‑150 (MQ=255)
                                                      aaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgg                                                                                           >  1:3336378/1‑150 (MQ=255)
                                                      aaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGGGTAAGAATTGGCGCGCCCACCGGCGGGGAGTGCTCGGTGTGTCCGGGCGGGATGACTACGGGCAACCCGGTCAATCCGCTGCTgg                                                                                           >  1:4507666/1‑150 (MQ=255)
                                                       aCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTggg                                                                                          <  1:3830499/150‑1 (MQ=255)
                                                            cggcgCGTCAGGGAGATCTGACTCAGTATGGCGGTCCCCGTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTACATCCGCTGCTGGGGGCgc                                                                                     >  1:3440534/1‑149 (MQ=255)
                                                            cggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGTGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGa                                                                                     >  1:3246420/1‑150 (MQ=255)
                                                            cggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGa                                                                                     >  1:364930/1‑150 (MQ=255)
                                                               cgcgTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAgg                                                                                  <  2:2862455/150‑1 (MQ=255)
                                                                 cgTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtg                                                                                >  2:3868584/1‑150 (MQ=255)
                                                                   tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgct                                                                              <  1:3672240/150‑1 (MQ=255)
                                                                    cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGTAGTGCACGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGTtgctg                                                                             >  1:5290208/1‑150 (MQ=255)
                                                                    cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctg                                                                             <  2:1075701/150‑1 (MQ=255)
                                                                    cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctg                                                                             >  1:4900415/1‑150 (MQ=255)
                                                                     aGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctgc                                                                            <  2:309035/150‑1 (MQ=255)
                                                                     aGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctgc                                                                            >  2:2622197/1‑150 (MQ=255)
                                                                      gggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGcc                                                                           >  2:446754/1‑150 (MQ=255)
                                                                          gaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGc                                                                       <  2:1643728/150‑1 (MQ=255)
                                                                             atGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCgag                                                                    <  2:3043094/150‑1 (MQ=255)
                                                                                aCTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACg                                                                 >  1:1008068/1‑150 (MQ=255)
                                                                                   cAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAc                                                              <  1:1249239/150‑1 (MQ=255)
                                                                                    aGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAcc                                                             <  2:4156460/150‑1 (MQ=255)
                                                                                       aTGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTg                                                          >  1:1297365/1‑150 (MQ=255)
                                                                                       aTGGCGGTCCCATTGTCCAGGGATCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGTCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCtag                                                          >  1:4797351/1‑148 (MQ=255)
                                                                                        tGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTgc                                                         <  1:3333339/150‑1 (MQ=255)
                                                                                           cGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCt                                                      <  1:4027487/150‑1 (MQ=255)
                                                                                             gTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgc                                                    >  2:5265486/1‑150 (MQ=255)
                                                                                               cccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgccc                                                  <  2:5013770/150‑1 (MQ=255)
                                                                                               cccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgccc                                                  <  2:4711292/150‑1 (MQ=255)
                                                                                                ccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccg                                                 >  1:2037683/1‑150 (MQ=255)
                                                                                                    tGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggccc                                             <  1:117054/150‑1 (MQ=255)
                                                                                                    tGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggccc                                             <  1:179724/150‑1 (MQ=255)
                                                                                                     gTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggcccg                                            >  2:2229326/1‑150 (MQ=255)
                                                                                                            gTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGt                                     <  2:2753567/150‑1 (MQ=255)
                                                                                                               cGGCATGTGTAAGAATAGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCTGCGAGACGGACCTTGCGCTGCCCGGCCCGCTCCCGTTCa                                  >  2:2411370/1‑150 (MQ=255)
                                                                                                                  cAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtc                               >  1:5115556/1‑150 (MQ=255)
                                                                                                                  cAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtc                               >  1:2682982/1‑150 (MQ=255)
                                                                                                                  cAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtc                               >  1:3099843/1‑150 (MQ=255)
                                                                                                                   aGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtct                              >  2:4265972/1‑150 (MQ=255)
                                                                                                                     gtgtAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctct                            >  1:2635217/1‑150 (MQ=255)
                                                                                                                     gtgtAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctct                            >  1:63245/1‑150 (MQ=255)
                                                                                                                       gtAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTcc                          >  1:1924396/1‑150 (MQ=255)
                                                                                                                        tAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTccc                         <  1:2959797/150‑1 (MQ=255)
                                                                                                                                ggCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAc                 <  1:22966/150‑1 (MQ=255)
                                                                                                                                ggCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAc                 >  1:1578746/1‑150 (MQ=255)
                                                                                                                                ggCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAc                 <  1:1880238/150‑1 (MQ=255)
                                                                                                                                  cgcgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGTAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcag               <  1:4150599/150‑1 (MQ=255)
                                                                                                                                    cgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcagca             >  2:4321070/1‑150 (MQ=255)
                                                                                                                                     gcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcagcag            <  2:3563031/150‑1 (MQ=255)
                                                                                                                                      cccACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACagcagc           >  2:1530441/1‑150 (MQ=255)
                                                                                                                                      cccACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACagcagc           >  1:4960443/1‑150 (MQ=255)
                                                                                                                                      cccACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACagcagc           >  1:2870026/1‑150 (MQ=255)
                                                                                                                                       ccACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCt          <  1:20773/150‑1 (MQ=255)
                                                                                                                                         aCCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTAc        >  1:1507531/1‑150 (MQ=255)
                                                                                                                                          ccGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTAcc       <  1:4299573/150‑1 (MQ=255)
                                                                                                                                             gcgtggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGa    <  2:309686/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACG  >  CP009273/518673‑518965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑