Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 535,073 C→A 24.9% I157I (ATC→ATA)  allB → allantoinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273535,0730CA24.9% 603.7 / 128.2 260I157I (ATC→ATA) allBallantoinase
Reads supporting (aligned to +/- strand):  ref base C (109/84);  new base A (34/33);  total (143/117)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

AAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTA  >  CP009273/534924‑535220
                                                                                                                                                     |                                                                                                                                                   
      gATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAAc                                                                                                                                               >  2:1923586/1‑150 (MQ=255)
       aTGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACg                                                                                                                                              <  1:422309/150‑1 (MQ=255)
             cTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATTGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTcc                                                                                                                                        <  2:5221702/150‑1 (MQ=255)
             cTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTcc                                                                                                                                        <  2:728466/150‑1 (MQ=255)
             cTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTcc                                                                                                                                        <  1:3469515/150‑1 (MQ=255)
              tAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCg                                                                                                                                       <  2:393914/150‑1 (MQ=255)
              tAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCg                                                                                                                                       >  1:2607097/1‑150 (MQ=255)
              tAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCg                                                                                                                                       <  2:534933/150‑1 (MQ=255)
                aaGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTg                                                                                                                                     >  2:158138/1‑150 (MQ=255)
                  ggCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGAt                                                                                                                                   <  2:3532516/150‑1 (MQ=255)
                   gCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATg                                                                                                                                  >  1:1116748/1‑150 (MQ=255)
                    cAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGt                                                                                                                                 <  1:2027343/150‑1 (MQ=255)
                        cTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAAc                                                                                                                             <  1:576299/150‑1 (MQ=255)
                           aCTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGAc                                                                                                                          >  2:4227417/1‑150 (MQ=255)
                            cTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACt                                                                                                                         >  2:1438439/1‑150 (MQ=255)
                               ttGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGc                                                                                                                      >  2:162535/1‑150 (MQ=255)
                                tGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCa                                                                                                                     >  1:761600/1‑150 (MQ=255)
                                 gATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAg                                                                                                                    <  2:2676086/150‑1 (MQ=255)
                                  aTGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGt                                                                                                                   >  1:4410033/1‑150 (MQ=255)
                                     cGGCACAACTCGGTGGCCTGGTGTCTTACAACCTCGACCGTCTGCATGAGCTGGATGATGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGTTCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGttct                                                                                                                <  2:457076/150‑1 (MQ=255)
                                     cGGCACAACTCGGGGGCGTGGTGTCTTACACCATCGCCCGTCTGCATGTGCTGGTTGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAAGGACTTCCGTGTTGTAAACGACTGGCAGttct                                                                                                                <  2:456889/150‑1 (MQ=255)
                                        cacaACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCa                                                                                                             <  2:797907/150‑1 (MQ=255)
                                         acaACTCGGGGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCaa                                                                                                            <  1:4825650/150‑1 (MQ=255)
                                          caACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCaaa                                                                                                           >  2:1272896/1‑150 (MQ=255)
                                            aCTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAgg                                                                                                         >  1:4977875/1‑150 (MQ=255)
                                             cTCGGTGGCCGGGTGTCTTACAACATCGACCGTCTGCAGGAGCTGGATGAAGTGGGCGTTGTCGGCTTAAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGAATTCCGTGTTGTAAACGACTGGCAGTTCTTCAAAGGt                                                                                                        <  1:2511215/150‑1 (MQ=255)
                                                ggTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTgcg                                                                                                     >  2:661253/1‑150 (MQ=255)
                                                  tGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCa                                                                                                   >  1:3172744/1‑150 (MQ=255)
                                                  tGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCa                                                                                                   >  2:2063837/1‑150 (MQ=255)
                                                   ggCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAg                                                                                                  >  1:1396464/1‑150 (MQ=255)
                                                       tGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGc                                                                                              >  2:2697959/1‑150 (MQ=255)
                                                        ggTGTATAACAACATCGACCGTCTGCATGAGCTGGTTGACGGGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCt                                                                                             <  1:3699821/150‑1 (MQ=255)
                                                         gtttattAAAAAATCGACCGTCTGATTGAGGTGGATGATGTGGGCGTTGTGGGCTTAAATTGTTTGGTTGCGACCTGTGGCGATGGCGTTTTAGACAACGACTTCCGTGTTGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTg                                                                                            <  1:5064238/145‑1 (MQ=255)
                                                         gtgtCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTg                                                                                            <  1:5265664/150‑1 (MQ=255)
                                                          tgtCTTAAAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGAATTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTgg                                                                                           <  1:840341/150‑1 (MQ=255)
                                                           gtCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAAAGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTggg                                                                                          >  1:2381144/1‑150 (MQ=255)
                                                               tACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGaa                                                                                      <  2:2142768/150‑1 (MQ=255)
                                                                 caacaTCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACt                                                                                    <  2:4356705/150‑1 (MQ=255)
                                                                    caTCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTggg                                                                                 >  1:2886447/1‑150 (MQ=255)
                                                                     aTCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGt                                                                                <  2:3814860/150‑1 (MQ=255)
                                                                      tCGACCGTCTGCATGAGCTGGATGATGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGTTGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTc                                                                               <  2:1825006/150‑1 (MQ=255)
                                                                       cGACCGTCTGCATGAGCTGGATGACGTGGGCTTTGTCGGCTACAAATGCTTCGTTGCGACCGGTGGCGATCGCGGTATCGACACCGACTCCCGTGATGTCAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCa                                                                              <  2:526147/150‑1 (MQ=255)
                                                                        gACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAg                                                                             >  2:3255000/1‑150 (MQ=255)
                                                                        gACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGAAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAg                                                                             >  1:1335902/1‑150 (MQ=255)
                                                                          ccGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGcc                                                                           >  2:4046684/1‑150 (MQ=255)
                                                                            gTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCgg                                                                         >  1:1923010/1‑150 (MQ=255)
                                                                             tCTGCATGAGCTGTATGAAGTGGGCGTTGTCGGCTTCAAATGCGTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGt                                                                        <  2:1061943/150‑1 (MQ=255)
                                                                               tGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGc                                                                      >  2:301310/1‑150 (MQ=255)
                                                                                   tGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGt                                                                  >  2:3673572/1‑150 (MQ=255)
                                                                                       cTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCAc                                                              >  2:4686394/1‑150 (MQ=255)
                                                                                        tGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACt                                                             <  2:2627384/150‑1 (MQ=255)
                                                                                        tGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACt                                                             >  2:2307018/1‑150 (MQ=255)
                                                                                        tGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACt                                                             <  2:4882660/150‑1 (MQ=255)
                                                                                        tGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACt                                                             >  1:3392654/1‑150 (MQ=255)
                                                                                          gATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTACGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGc                                                           >  2:3041899/1‑150 (MQ=255)
                                                                                           aTGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGATGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCg                                                          >  2:5283385/1‑150 (MQ=255)
                                                                                             gAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGaa                                                        >  1:425918/1‑150 (MQ=255)
                                                                                              atgTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGaaa                                                       <  1:2110548/148‑1 (MQ=255)
                                                                                              aaGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGaaa                                                       >  2:1632721/1‑150 (MQ=255)
                                                                                                gTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAc                                                     <  1:2625191/150‑1 (MQ=255)
                                                                                                 tGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAcg                                                    >  1:1249177/1‑150 (MQ=255)
                                                                                                  gggCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAcgc                                                   >  1:4094019/1‑150 (MQ=255)
                                                                                                     cGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCt                                                <  1:4734721/150‑1 (MQ=255)
                                                                                                       ttGTCGTCTTCAACTGCTGCGTTGCGACCTGTGGCGTGCGCGGTATCGACAACTACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGa                                              <  1:4469123/150‑1 (MQ=255)
                                                                                                       ttGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGa                                              >  1:2564843/1‑150 (MQ=255)
                                                                                                       ttGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGa                                              >  1:2999177/1‑150 (MQ=255)
                                                                                                        tGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGAt                                             <  2:4811049/150‑1 (MQ=255)
                                                                                                          tCGGCTTCAACTGCTTCGTGGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGAttt                                           <  1:2849776/150‑1 (MQ=255)
                                                                                                          tCGGATTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGAttt                                           <  1:1738073/150‑1 (MQ=255)
                                                                                                           cGGATTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCCACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTtg                                          <  1:1437912/150‑1 (MQ=255)
                                                                                                            ggCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTtgt                                         >  1:1702825/1‑150 (MQ=255)
                                                                                                            ggCTTCAAATGCTTCGGTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTtgt                                         <  2:2952858/150‑1 (MQ=255)
                                                                                                             gCTTCAATTGCTTCGTTGCGACCTGTGGCGAGCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTtgtg                                        <  1:1073012/150‑1 (MQ=255)
                                                                                                              cTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGa                                       >  2:26377/1‑150 (MQ=255)
                                                                                                               ttCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGAc                                      >  2:1720951/1‑150 (MQ=255)
                                                                                                               ttCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGAc                                      >  1:547358/1‑150 (MQ=255)
                                                                                                                  aaaTGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGaa                                   >  1:1597871/1‑150 (MQ=255)
                                                                                                                  aaaTGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGaa                                   >  1:2902733/1‑150 (MQ=255)
                                                                                                                   attGCTTGGTGGCGACCTGGGGCGATCGGGTTATCGACAACGACTTCGGTTTTTAACACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAAc                                  <  1:2287343/148‑1 (MQ=255)
                                                                                                                    aTGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACt                                 >  2:2039722/1‑150 (MQ=255)
                                                                                                                     tGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTg                                >  2:4272181/1‑150 (MQ=255)
                                                                                                                      gCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTgg                               <  1:2165022/150‑1 (MQ=255)
                                                                                                                       cTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTggg                              >  1:5161628/1‑150 (MQ=255)
                                                                                                                          cGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCga                           >  1:4030591/1‑150 (MQ=255)
                                                                                                                           gTTGCGACCTGAGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGATGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCgaa                          >  1:2732622/1‑150 (MQ=255)
                                                                                                                             tGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCgaaga                        >  1:2756587/1‑150 (MQ=255)
                                                                                                                               cGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGaagaag                      >  2:2426168/1‑150 (MQ=255)
                                                                                                                                gACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagc                     >  1:2434014/1‑150 (MQ=255)
                                                                                                                                  ccTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcga                   <  1:3493058/150‑1 (MQ=255)
                                                                                                                                     gtgGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcgaagc                <  2:4398105/150‑1 (MQ=255)
                                                                                                                                       ggCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGt              >  2:2551535/1‑150 (MQ=255)
                                                                                                                                        gCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTg             <  2:5095794/150‑1 (MQ=255)
                                                                                                                                          gATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGaa           >  1:959660/1‑150 (MQ=255)
                                                                                                                                           aTCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAg          >  1:4545000/1‑150 (MQ=255)
                                                                                                                                              ggggTATCGACAACGACTTCCGTGATGTACACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTc       <  1:3707867/148‑1 (MQ=255)
                                                                                                                                              gcgGTATCCACAACTACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTc       <  1:1812300/150‑1 (MQ=255)
                                                                                                                                               cgGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTcg      >  1:3196918/1‑150 (MQ=255)
                                                                                                                                               cgGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTcg      >  1:4053484/1‑150 (MQ=255)
                                                                                                                                                ggTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTcgc     >  2:4380536/1‑150 (MQ=255)
                                                                                                                                                   aTCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTa  >  1:1141390/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTA  >  CP009273/534924‑535220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑