Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,592 G→T 100% D814Y (GAC→TAC)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,5920GT100.0% 899.5 / NA 273D814Y (GAC→TAC) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (134/139);  total (134/139)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 275 total aligned reads displayed.

CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGC  >  CP009273/557445‑557738
                                                                                                                                                   |                                                                                                                                                  
 cGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACg                                                                                                                                                 <  1:3415475/150‑1 (MQ=255)
 cGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACg                                                                                                                                                 <  2:5088009/150‑1 (MQ=255)
 cGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACg                                                                                                                                                 <  2:4746907/150‑1 (MQ=255)
  gACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACgg                                                                                                                                                >  1:3284368/1‑150 (MQ=255)
  gACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACgg                                                                                                                                                >  2:2050744/1‑150 (MQ=255)
   aCGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACggg                                                                                                                                               <  2:574664/150‑1 (MQ=255)
   aCGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACggg                                                                                                                                               <  2:4841405/150‑1 (MQ=255)
    cGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGc                                                                                                                                              >  1:3118532/1‑150 (MQ=255)
       aaGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAg                                                                                                                                           <  2:227267/150‑1 (MQ=255)
       aaGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAg                                                                                                                                           >  2:2761556/1‑150 (MQ=255)
          gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGttt                                                                                                                                        <  1:1809512/150‑1 (MQ=255)
              gTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttattt                                                                                                                                    >  1:4716969/1‑150 (MQ=255)
                 ggTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAg                                                                                                                                 <  2:3101070/150‑1 (MQ=255)
                 ggTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAg                                                                                                                                 >  1:90626/1‑150 (MQ=255)
                  gTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGGTTATTTAAgt                                                                                                                                >  1:1560458/1‑150 (MQ=255)
                   ttCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtg                                                                                                                               <  1:745789/150‑1 (MQ=255)
                     cGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggt                                                                                                                             <  1:2358535/150‑1 (MQ=255)
                      gTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggtg                                                                                                                            >  2:1668335/1‑150 (MQ=255)
                      gTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggtg                                                                                                                            <  2:1602650/150‑1 (MQ=255)
                      gTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggtg                                                                                                                            >  2:3440614/1‑150 (MQ=255)
                      gTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggtg                                                                                                                            <  1:617238/150‑1 (MQ=255)
                       tGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTgc                                                                                                                           <  2:4274594/150‑1 (MQ=255)
                           aaTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTCTGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                       >  2:2141607/1‑150 (MQ=255)
                           aaTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                       <  2:1495829/150‑1 (MQ=255)
                           aaTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                       <  1:1250249/150‑1 (MQ=255)
                           aaTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                       >  1:1204423/1‑150 (MQ=255)
                            aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAt                                                                                                                      >  2:1022687/1‑150 (MQ=255)
                             ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAtt                                                                                                                     <  2:1463526/150‑1 (MQ=255)
                              tttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTg                                                                                                                    <  1:4386159/150‑1 (MQ=255)
                                tGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTc                                                                                                                  <  1:1113246/150‑1 (MQ=255)
                                 gATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCt                                                                                                                 <  2:2032095/150‑1 (MQ=255)
                                         cATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAtt                                                                                                         <  1:2270172/150‑1 (MQ=255)
                                         cATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAtt                                                                                                         <  1:3308372/150‑1 (MQ=255)
                                          aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                        >  1:1266338/1‑150 (MQ=255)
                                          aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                        >  1:350099/1‑150 (MQ=255)
                                          aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                        <  2:4957690/150‑1 (MQ=255)
                                            aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGGGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACt                                                                                                      >  1:1894707/1‑150 (MQ=255)
                                                gTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTTGTGCGCCATTGTCTTGTGAATTACTGGtt                                                                                                  >  2:650280/1‑150 (MQ=255)
                                                gTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGtt                                                                                                  <  2:5170281/150‑1 (MQ=255)
                                                 tGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTc                                                                                                 <  1:4185465/150‑1 (MQ=255)
                                                  gCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCa                                                                                                <  2:2900493/150‑1 (MQ=255)
                                                     ggCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTg                                                                                             >  1:2878570/1‑150 (MQ=255)
                                                        tcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTgggg                                                                                          <  1:3741614/149‑1 (MQ=255)
                                                        gcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTgggg                                                                                          <  2:4027387/150‑1 (MQ=255)
                                                        gcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTgggg                                                                                          >  1:2233323/1‑150 (MQ=255)
                                                           cATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGgaga                                                                                       >  2:1407462/1‑150 (MQ=255)
                                                             ttACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACg                                                                                     <  1:1954123/150‑1 (MQ=255)
                                                               aCCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgc                                                                                   >  2:3555328/1‑150 (MQ=255)
                                                               aCCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgc                                                                                   >  1:1027748/1‑150 (MQ=255)
                                                               aCCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgc                                                                                   >  2:1307185/1‑150 (MQ=255)
                                                                ccGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcg                                                                                  <  1:492641/150‑1 (MQ=255)
                                                                  gTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGcgcg                                                                                >  2:3975208/1‑150 (MQ=255)
                                                                   ttACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGa                                                                               >  1:4129311/1‑150 (MQ=255)
                                                                    tACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGaa                                                                              >  2:5108189/1‑150 (MQ=255)
                                                                    tACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGaa                                                                              >  1:4235810/1‑150 (MQ=255)
                                                                     aCCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAAc                                                                             <  1:434847/150‑1 (MQ=255)
                                                                      cccAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACt                                                                            <  2:581971/150‑1 (MQ=255)
                                                                         aGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCAc                                                                         <  1:944742/150‑1 (MQ=255)
                                                                          gggCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACg                                                                        <  1:2923972/150‑1 (MQ=255)
                                                                              ggAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGc                                                                    >  1:2455421/1‑150 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                 <  1:4001742/150‑1 (MQ=255)
                                                                                    ccGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTgcg                                                              <  1:5288533/150‑1 (MQ=255)
                                                                                           cGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATg                                                       <  2:4447932/150‑1 (MQ=255)
                                                                                            gTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGt                                                      >  2:3646549/1‑150 (MQ=255)
                                                                                                ggATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTg                                                  <  2:246886/150‑1 (MQ=255)
                                                                                                  aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                                <  2:2547012/150‑1 (MQ=255)
                                                                                                  aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                                <  2:3410683/150‑1 (MQ=255)
                                                                                                  aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGAGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                                <  2:3066674/150‑1 (MQ=255)
                                                                                                     aCTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGaa                                             >  1:3175855/1‑150 (MQ=255)
                                                                                                     aCTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGaa                                             <  1:4459761/150‑1 (MQ=255)
                                                                                                       tGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGc                                           <  1:2005967/150‑1 (MQ=255)
                                                                                                             gggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCt                                     <  1:465847/150‑1 (MQ=255)
                                                                                                               gAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTa                                   >  1:3937219/1‑150 (MQ=255)
                                                                                                               gAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTa                                   >  1:3869366/1‑150 (MQ=255)
                                                                                                                aaaaCAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTAc                                  >  1:5083367/1‑150 (MQ=255)
                                                                                                                 aaaCAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACa                                 >  2:1199146/1‑150 (MQ=255)
                                                                                                                     aGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCaa                             >  2:2376313/1‑150 (MQ=255)
                                                                                                                      gTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAg                            >  2:2559888/1‑150 (MQ=255)
                                                                                                                         ccGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCg                         >  1:1643990/1‑150 (MQ=255)
                                                                                                                          cGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGt                        <  2:2934711/150‑1 (MQ=255)
                                                                                                                             aaTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAc                     >  1:1352602/1‑150 (MQ=255)
                                                                                                                              aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                    <  1:1473114/150‑1 (MQ=255)
                                                                                                                              aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                    <  1:1848148/150‑1 (MQ=255)
                                                                                                                              aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                    >  1:957961/1‑150 (MQ=255)
                                                                                                                                aaCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                  >  2:3221415/1‑150 (MQ=255)
                                                                                                                                 aCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGtt                 <  1:813461/150‑1 (MQ=255)
                                                                                                                                  ccAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCCGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTa                >  1:410557/1‑150 (MQ=255)
                                                                                                                                   cAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAt               <  1:3308595/150‑1 (MQ=255)
                                                                                                                                    aGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTTTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAtt              >  2:3363434/1‑150 (MQ=255)
                                                                                                                                      tATGGTGGGTGATTACGGGCAAGTTTATTTCAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGTACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc            <  2:1294138/150‑1 (MQ=255)
                                                                                                                                      tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc            >  1:636666/1‑150 (MQ=255)
                                                                                                                                      tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc            <  1:1408325/150‑1 (MQ=255)
                                                                                                                                       aTGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCg           <  2:5223725/150‑1 (MQ=255)
                                                                                                                                        tggtggGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCgg          <  1:2538613/150‑1 (MQ=255)
                                                                                                                                          gtgggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCa        <  1:1688627/150‑1 (MQ=255)
                                                                                                                                           tgggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAg       <  2:734107/150‑1 (MQ=255)
                                                                                                                                            gggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGt      <  2:2830987/150‑1 (MQ=255)
                                                                                                                                            gggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGt      <  2:4877758/150‑1 (MQ=255)
                                                                                                                                              gtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGttt    >  2:3799932/1‑150 (MQ=255)
                                                                                                                                              gtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATATATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGttt    >  1:4505582/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGC  >  CP009273/557445‑557738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑