BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	583,341	T→G	100%	S33R (AGT→CGT)	ybcH ←	PRK09936 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	583,341	0	T	G	100.0%	1206.0 / NA	301	S33R (AGT→CGT)	ybcH	PRK09936 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (144/157); total (144/157)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 301 total aligned reads displayed.

CAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTAC  >  CP009273/583194‑583487

cAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAt                                                                                                                                                  >  2:4794796/1‑150 (MQ=255)

       ggCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtt                                                                                                                                           >  1:2133084/1‑150 (MQ=255)

         cccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtttt                                                                                                                                         <  1:323144/150‑1 (MQ=255)

           cGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtgt                                                                                                                                       >  2:870548/1‑150 (MQ=255)

            gCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtgtg                                                                                                                                      <  1:4014803/150‑1 (MQ=255)

               tAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGtt                                                                                                                                   <  1:730616/150‑1 (MQ=255)

               tAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGtt                                                                                                                                   <  2:107774/150‑1 (MQ=255)

                aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                  <  1:2725213/150‑1 (MQ=255)

                 aaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGc                                                                                                                                 >  1:2016794/1‑150 (MQ=255)

                   cAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCa                                                                                                                               >  2:2995876/1‑150 (MQ=255)

                      tAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTTTCGGTAACCTGATGATCTCGGTTTTGTGGTTGCCAGat                                                                                                                            <  1:494558/150‑1 (MQ=255)

                      tAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGat                                                                                                                            >  2:4445694/1‑150 (MQ=255)

                       aaCGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGata                                                                                                                           <  2:2870430/150‑1 (MQ=255)

                       aaCGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGata                                                                                                                           >  1:100478/1‑150 (MQ=255)

                          gTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGataata                                                                                                                        <  1:518019/150‑1 (MQ=255)

                           tGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATAc                                                                                                                       <  2:4865926/150‑1 (MQ=255)

                             cgcTGTTCTGGCTGGGTCAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTTTCGCAGCCGTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGTTCTCGGTTTTGTGGTGGGCAGATAGTAcgt                                                                                                                     >  2:4356476/1‑148 (MQ=255)

                             cgcTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCt                                                                                                                     <  2:1628737/150‑1 (MQ=255)

                              gcTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCtt                                                                                                                    <  2:2066782/150‑1 (MQ=255)

                               cTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCttt                                                                                                                   <  2:3373867/150‑1 (MQ=255)

                                 gTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCa                                                                                                                 <  1:565626/150‑1 (MQ=255)

                                 gTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCa                                                                                                                 >  1:4327438/1‑150 (MQ=255)

                                  ttCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCAt                                                                                                                <  1:3520170/150‑1 (MQ=255)

                                  ttCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCAt                                                                                                                >  1:44878/1‑150 (MQ=255)

                                   tCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATc                                                                                                               <  1:26603/150‑1 (MQ=255)

                                   tCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATc                                                                                                               >  1:1461865/1‑150 (MQ=255)

                                        ctggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCaaa                                                                                                          >  1:2949172/1‑150 (MQ=255)

                                          gggTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAgg                                                                                                        <  2:2193010/150‑1 (MQ=255)

                                            gTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaa                                                                                                      <  1:5007843/150‑1 (MQ=255)

                                                 tGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAgg                                                                                                 <  1:3851456/150‑1 (MQ=255)

                                                  gCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAggg                                                                                                <  2:2117960/150‑1 (MQ=255)

                                                   cATCGCCGTAACGGGTCCATTGCAAAACAGGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGc                                                                                               >  2:3356057/1‑150 (MQ=255)

                                                    aTCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCt                                                                                              <  1:5137479/150‑1 (MQ=255)

                                                    aTCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCt                                                                                              <  2:4223486/150‑1 (MQ=255)

                                                    aTCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCt                                                                                              >  2:4251585/1‑150 (MQ=255)

                                                            aaCGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaaa                                                                                      >  1:1130943/1‑150 (MQ=255)

                                                            aaCGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaaa                                                                                      >  1:1240821/1‑150 (MQ=255)

                                                            aaCGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaaa                                                                                      >  1:3325236/1‑150 (MQ=255)

                                                             aCGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAg                                                                                     <  1:4093604/150‑1 (MQ=255)

                                                                 gTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgt                                                                                 <  1:5258191/150‑1 (MQ=255)

                                                                 gTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgt                                                                                 >  2:125283/1‑150 (MQ=255)

                                                                 gTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgt                                                                                 <  2:115024/150‑1 (MQ=255)

                                                                    cATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTc                                                                              >  2:1535085/1‑150 (MQ=255)

                                                                     aTTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCa                                                                             <  1:630722/150‑1 (MQ=255)

                                                                     aTTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCa                                                                             <  1:4941200/150‑1 (MQ=255)

                                                                     aTTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCa                                                                             >  1:2255982/1‑150 (MQ=255)

                                                                     aTTGCAAAACAAGGGTATCGAAGCCTTGAAAACGTAACAGACTCATCAACCCCTGCCACTGGGTAAAGGTAACCTGACGATCTCGGTTTTGTGGTTGACAGATAATACCAATCATCTCAAAGGAAAAAGTGCTGACCAAAAGCAGAGTCa                                                                             >  1:1580581/1‑150 (MQ=255)

                                                                      ttGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAg                                                                            <  1:3113008/150‑1 (MQ=255)

                                                                       tGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGc                                                                           <  1:1808919/150‑1 (MQ=255)

                                                                        gCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCa                                                                          <  2:818936/150‑1 (MQ=255)

                                                                        gCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCa                                                                          <  2:4635295/150‑1 (MQ=255)

                                                                          aaaaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAAt                                                                        <  1:487544/150‑1 (MQ=255)

                                                                           aaaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATa                                                                       <  2:4431141/150‑1 (MQ=255)

                                                                            aaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                      <  2:2928121/150‑1 (MQ=255)

                                                                            aaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                      >  2:1450297/1‑150 (MQ=255)

                                                                            aaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                      <  1:2340860/150‑1 (MQ=255)

                                                                            aaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                      <  1:998834/150‑1 (MQ=255)

                                                                            aaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                      <  1:4905258/150‑1 (MQ=255)

                                                                            aaCAAGGGTATCGAAGCCTTGCAAACGCAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                      <  2:4150611/150‑1 (MQ=255)

                                                                             aCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACg                                                                     <  2:2327933/150‑1 (MQ=255)

                                                                              cAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGa                                                                    >  1:2383077/1‑150 (MQ=255)

                                                                               aaGGGTATCGAAGCATTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaa                                                                   <  2:4717603/150‑1 (MQ=255)

                                                                                aGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTATGTGGTTGCCAGATAATACATTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaa                                                                  >  2:3703823/1‑150 (MQ=255)

                                                                                   gTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATg                                                               <  1:4251585/150‑1 (MQ=255)

                                                                                     aTCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGaa                                                             <  2:4002207/150‑1 (MQ=255)

                                                                                     aTCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGaa                                                             <  2:374480/150‑1 (MQ=255)

                                                                                       cGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACt                                                           >  2:1482406/1‑150 (MQ=255)

                                                                                        gAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACtt                                                          >  1:1711702/1‑150 (MQ=255)

                                                                                               tGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAtt                                                   <  2:1225811/150‑1 (MQ=255)

                                                                                                gCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAttt                                                  <  2:2016794/150‑1 (MQ=255)

                                                                                                gCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAttt                                                  >  1:1816917/1‑150 (MQ=255)

                                                                                                 cAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTa                                                 <  2:577471/150‑1 (MQ=255)

                                                                                                  aaaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAc                                                <  1:3958220/150‑1 (MQ=255)

                                                                                                  aaaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAc                                                >  2:3262859/1‑150 (MQ=255)

                                                                                                       tAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTg                                           >  2:3525673/1‑150 (MQ=255)

                                                                                                       tAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTg                                           >  1:2460130/1‑150 (MQ=255)

                                                                                                        aaCTGACTCATCAGCCCCTGCCACTTGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGAAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGc                                          <  1:345483/150‑1 (MQ=255)

                                                                                                        aaCTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGc                                          <  2:4976681/150‑1 (MQ=255)

                                                                                                                catcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAAt                                  >  1:5052366/1‑150 (MQ=255)

                                                                                                                catcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAAt                                  <  2:3785972/150‑1 (MQ=255)

                                                                                                                 atcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATg                                 >  1:2185526/1‑150 (MQ=255)

                                                                                                                   caGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGt                               >  2:4891264/1‑150 (MQ=255)

                                                                                                                   caGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGt                               >  1:2847786/1‑150 (MQ=255)

                                                                                                                   caGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGt                               >  2:1802783/1‑150 (MQ=255)

                                                                                                                       cccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTgaga                           >  1:3927605/1‑150 (MQ=255)

                                                                                                                        ccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGaa                          >  1:3179904/1‑150 (MQ=255)

                                                                                                                        ccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGaa                          >  2:4812644/1‑150 (MQ=255)

                                                                                                                           gCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAcg                       >  2:5222733/1‑150 (MQ=255)

                                                                                                                             cACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAcgcg                     <  1:4402377/150‑1 (MQ=255)

                                                                                                                                tGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgttg                  >  2:2174002/1‑150 (MQ=255)

                                                                                                                                tGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgttg                  >  1:3373030/1‑150 (MQ=255)

                                                                                                                                   gTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGttgttg               >  1:3641339/1‑150 (MQ=255)

                                                                                                                                    tATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTgc              >  1:86338/1‑150 (MQ=255)

                                                                                                                                        ggTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGctc          <  2:2708170/150‑1 (MQ=255)

                                                                                                                                           aaCCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTcc       >  1:4726373/1‑150 (MQ=255)

                                                                                                                                           aaCCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTcc       >  1:1325415/1‑150 (MQ=255)

                                                                                                                                             ccTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGt     >  2:2826959/1‑150 (MQ=255)

                                                                                                                                             ccTGACGATCTCGGTTTTGTGGTTGCCAGAAAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGt     <  2:2294127/150‑1 (MQ=255)

                                                                                                                                                gACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTAc  <  2:2151109/150‑1 (MQ=255)

                                                                                                                                                gACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTAc  <  2:1336339/150‑1 (MQ=255)

CAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTAC  >  CP009273/583194‑583487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`