Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 608,575 T→A 24.7% L128Q (CTG→CAG)  fes → enterobactin/ferric enterobactin esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273608,5750TA24.7% 563.2 / 210.3 308L128Q (CTG→CAG) fesenterobactin/ferric enterobactin esterase
Reads supporting (aligned to +/- strand):  ref base T (107/124);  new base A (40/36);  total (147/161)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

Only 100 of 308 total aligned reads displayed.

TGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGTTGAAAAAG  >  CP009273/608427‑608723
                                                                                                                                                    |                                                                                                                                                    
tGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTg                                                                                                                                                     >  2:1528780/1‑150 (MQ=255)
 ggACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGa                                                                                                                                                    >  2:3038477/1‑150 (MQ=255)
   acgacACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAAc                                                                                                                                                  <  1:2804836/150‑1 (MQ=255)
   acgacACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAAc                                                                                                                                                  <  2:2944815/150‑1 (MQ=255)
    cgacACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAAcc                                                                                                                                                 >  1:2874299/1‑150 (MQ=255)
       cacaACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTAc                                                                                                                                              <  1:76238/150‑1 (MQ=255)
       cacaACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTAc                                                                                                                                              >  2:3647916/1‑150 (MQ=255)
         caACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACaa                                                                                                                                            >  1:827145/1‑150 (MQ=255)
           aCTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAg                                                                                                                                          <  2:2333506/150‑1 (MQ=255)
                aTGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGa                                                                                                                                     <  1:2748246/150‑1 (MQ=255)
                aTGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGa                                                                                                                                     <  2:2574710/150‑1 (MQ=255)
                         ggCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAggcgggc                                                                                                                            <  2:2210870/150‑1 (MQ=255)
                         ggCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAggcgggc                                                                                                                            <  2:4242438/150‑1 (MQ=255)
                         ggCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAAATGCGCGAAGGCTGGCGAAAACAATTACCCCAGGCGAAAGCCGATCCGCTGAACCTACAAAGCTGGAAAggcgggc                                                                                                                            >  1:1774199/1‑150 (MQ=255)
                          gcgcGGCAGCTTCTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCTCGAAGGATGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAATggcgggcg                                                                                                                           <  2:2652958/150‑1 (MQ=255)
                          gcgcGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAggcgggcg                                                                                                                           >  1:4902006/1‑150 (MQ=255)
                          gcgcGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAggcgggcg                                                                                                                           <  2:4538616/150‑1 (MQ=255)
                           cgcgGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGa                                                                                                                          >  2:903499/1‑150 (MQ=255)
                             cgGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAgg                                                                                                                        <  2:3059955/150‑1 (MQ=255)
                              ggCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAggg                                                                                                                       >  2:3472529/1‑150 (MQ=255)
                              ggCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAggg                                                                                                                       >  1:3442622/1‑150 (MQ=255)
                                 aGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCAc                                                                                                                    >  2:2102736/1‑150 (MQ=255)
                                 aGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCAc                                                                                                                    >  2:2378832/1‑150 (MQ=255)
                                 aGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCAc                                                                                                                    >  1:4856933/1‑150 (MQ=255)
                                 aGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCAc                                                                                                                    <  2:3878574/150‑1 (MQ=255)
                                  gCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACg                                                                                                                   <  1:2214877/150‑1 (MQ=255)
                                    tACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCt                                                                                                                 <  2:565644/150‑1 (MQ=255)
                                    tACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCt                                                                                                                 <  2:4012258/150‑1 (MQ=255)
                                    tACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCt                                                                                                                 <  1:2379178/150‑1 (MQ=255)
                                          tttATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCt                                                                                                           >  2:2790347/1‑150 (MQ=255)
                                          tttATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCt                                                                                                           <  2:704060/150‑1 (MQ=255)
                                           ttATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTg                                                                                                          >  2:286934/1‑150 (MQ=255)
                                              ttCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCAc                                                                                                       >  1:3761640/1‑150 (MQ=255)
                                                ccccccGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTc                                                                                                     <  2:3626537/150‑1 (MQ=255)
                                                 ccACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAAACTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCg                                                                                                    >  1:1387267/1‑150 (MQ=255)
                                                  cACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGa                                                                                                   >  1:2671869/1‑150 (MQ=255)
                                                    ccGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGaaa                                                                                                 >  2:3820570/1‑150 (MQ=255)
                                                    ccGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGaaa                                                                                                 >  2:2606732/1‑150 (MQ=255)
                                                     cGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAAt                                                                                                <  2:4739764/150‑1 (MQ=255)
                                                     cGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAAt                                                                                                <  2:1446101/150‑1 (MQ=255)
                                                      gAACGCGATGACATTTGTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATg                                                                                               <  2:4681737/150‑1 (MQ=255)
                                                       aaCGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGc                                                                                              >  1:1222699/1‑150 (MQ=255)
                                                       aaCGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGc                                                                                              >  1:3689754/1‑150 (MQ=255)
                                                             aTGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAg                                                                                        <  1:1962685/150‑1 (MQ=255)
                                                              tGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAgc                                                                                       >  1:5101589/1‑150 (MQ=255)
                                                                  aTTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCct                                                                                   >  2:4101212/1‑150 (MQ=255)
                                                                          tGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAAcc                                                                           >  2:3658182/1‑150 (MQ=255)
                                                                             aCCATCCCCCGATCGCCTCTATTTGCGCTAAGGATGGCTAAAACTATTACCCCAGGCTATAGCCTATCCGCATAACCTACAAAGCTGGAAAGGGGGGCGCGGGCAGGCTTTTTCTGAACTCTAATTGCCGCAAGCGCCTCTGCAACCggg                                                                        <  1:2084768/150‑1 (MQ=255)
                                                                               cATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCgggat                                                                      <  1:3109809/150‑1 (MQ=255)
                                                                               cATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCgggat                                                                      <  2:5057057/150‑1 (MQ=255)
                                                                                aTCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCgggatg                                                                     >  2:2257384/1‑150 (MQ=255)
                                                                                  cccccGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCgggatggg                                                                   >  2:1037970/1‑150 (MQ=255)
                                                                                     ccGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGAtt                                                                <  1:2790347/150‑1 (MQ=255)
                                                                                      cGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTg                                                               >  1:3287278/1‑150 (MQ=255)
                                                                                        aTCGCCTCGAATTGCGCGCAGGCTGGCGAAAACTATTCCCCCAGGCGATAGCCGATCCGCAGAACCTACACAGCTGGAAAGGCGGGCGAGGGCACGCTGTTACTGCACTCGAAATGCCGCAAGCGCCTCTGCTACCGGGATGGGATTGTc                                                             <  2:3107710/150‑1 (MQ=255)
                                                                                        aTCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTc                                                             <  2:4804466/150‑1 (MQ=255)
                                                                                         tCGCCTCGACTGGCGCGACGGCTGGCGAAAACTATTCCCCCCGGCGATAGCCGATCCGCTGACCCTACACAGCTGGAACGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCACGCGCCTCTGCAACCGGGATGGGATTGTcc                                                            <  1:1006063/150‑1 (MQ=255)
                                                                                         tCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTcc                                                            >  2:1188085/1‑150 (MQ=255)
                                                                                          cGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCg                                                           <  1:895894/150‑1 (MQ=255)
                                                                                           gCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGc                                                          >  1:5300753/1‑150 (MQ=255)
                                                                                              tcgacgggcgcGAAGGCTGGCGACAACTCTGCCCCCCGGCGCGAGCCGACCCGCAGAACCGACAAAGCTGGAACGGCGGGGGTGGGCACGCGGTTTCTGCACTCGAACTGCCGCAAGCGCCTCTGCAACCGGGATGGGAGTGTCCGCAAg                                                       <  2:3584487/143‑1 (MQ=255)
                                                                                                gAATTGCGCGAAGGCTGGCTAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAgcg                                                     <  2:4404449/150‑1 (MQ=255)
                                                                                                     gcgcGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGa                                                <  2:1010507/150‑1 (MQ=255)
                                                                                                       gcgAAGGCTGGCGAAAACTATTCCCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGaaa                                              <  2:4934574/150‑1 (MQ=255)
                                                                                                          aaGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCCGAAATAc                                           >  2:1600688/1‑150 (MQ=255)
                                                                                                             gCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTg                                        <  1:1131256/150‑1 (MQ=255)
                                                                                                                ggCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCa                                     <  1:3068769/150‑1 (MQ=255)
                                                                                                                ggCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCa                                     <  1:4696806/150‑1 (MQ=255)
                                                                                                                  cGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCaaa                                   >  2:1242387/1‑150 (MQ=255)
                                                                                                                   gAAAACTATTCCCCCCGGCGATAGCCGAACCGCTTAACCTAAAAAGCTGGAATGGCGGGCGTGGGCACGCTGTTTCTGCACTCTAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAg                                  <  1:3129996/150‑1 (MQ=255)
                                                                                                                   gAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACACAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAg                                  <  2:2440113/150‑1 (MQ=255)
                                                                                                                    aaaaCTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGa                                 <  2:2363002/150‑1 (MQ=255)
                                                                                                                    aaaaCTAGTCCCCCCGGCGATAGCCGATCCGCGGAACCTACAAAGCGGGATCGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGa                                 <  2:1423918/150‑1 (MQ=255)
                                                                                                                     aaaCTATTACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGaa                                >  2:2711993/1‑150 (MQ=255)
                                                                                                                        cTATTACCCCAGGCGATAGCCGATCCGCTGAAACTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAAACGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCAAAAGAAAtt                             >  1:1190553/1‑150 (MQ=255)
                                                                                                                         tATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTa                            >  2:4856923/1‑150 (MQ=255)
                                                                                                                         tATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTa                            >  1:4141120/1‑150 (MQ=255)
                                                                                                                         tATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTa                            >  1:3973916/1‑150 (MQ=255)
                                                                                                                         tATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTa                            >  1:2933668/1‑150 (MQ=255)
                                                                                                                           ttACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATc                          >  2:3243810/1‑150 (MQ=255)
                                                                                                                            tACCCCAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCt                         >  1:4637539/1‑150 (MQ=255)
                                                                                                                              ccccCGGCGATAGCCGATCCGCTGACCCTACAACGCTGGAACGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTgg                       <  1:4303320/150‑1 (MQ=255)
                                                                                                                              ccccAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTCTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTgg                       <  2:4326212/150‑1 (MQ=255)
                                                                                                                                 cAGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGaaa                    <  1:3865328/150‑1 (MQ=255)
                                                                                                                                  aGGCGATAGCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGaaaa                   >  1:254387/1‑150 (MQ=255)
                                                                                                                                   ggCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAg                  <  1:5189480/150‑1 (MQ=255)
                                                                                                                                      gATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGACAAGTGa               >  2:5244939/1‑150 (MQ=255)
                                                                                                                                      gATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGa               >  2:2031969/1‑150 (MQ=255)
                                                                                                                                        tAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAAc             <  2:827726/150‑1 (MQ=255)
                                                                                                                                          gCCGTTCCGCTGAACATACAAAGCTTGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACgg           <  2:1774199/150‑1 (MQ=255)
                                                                                                                                          gCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACgg           >  1:4360924/1‑150 (MQ=255)
                                                                                                                                          gCCGATCCGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACgg           >  1:1522777/1‑150 (MQ=255)
                                                                                                                                           ccGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGt          <  2:484550/150‑1 (MQ=255)
                                                                                                                                            cGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGtt         >  1:1581783/1‑150 (MQ=255)
                                                                                                                                             gATCCGCTGACCCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGTTg        <  2:1922232/150‑1 (MQ=255)
                                                                                                                                               tCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGTTGaa      >  2:3385968/1‑150 (MQ=255)
                                                                                                                                                ccGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCGGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGTTGaaa     <  2:1656909/150‑1 (MQ=255)
                                                                                                                                                ccGCAGAACCTACAAAGCTGGAAAGGCGGGCGTGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGTTGaaa     <  1:4533876/150‑1 (MQ=255)
                                                                                                                                                ccGCAGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGTTGaaa     <  1:2475920/150‑1 (MQ=255)
                                                                                                                                                  gCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGTTGaaaaa   >  1:2596262/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATGCCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAAGAAATTATCTGGAAAAGTGAACGGTTGAAAAAG  >  CP009273/608427‑608723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑