Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 667,899 C→A 100% D781Y (GAT→TAT)  leuS ← leucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273667,8990CA99.6% 855.9 / ‑4.5 266D781Y (GAT→TAT) leuSleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (119/146);  total (119/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 303 total aligned reads displayed.

CAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCA  >  CP009273/667752‑668045
                                                                                                                                                   |                                                                                                                                                  
cAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagc                                                                                                                                                  >  1:3206228/1‑150 (MQ=255)
 aGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcc                                                                                                                                                 >  1:4637587/1‑150 (MQ=255)
  gCACGTTCGCGAACCTGTTCTTCCGGTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcct                                                                                                                                                <  1:4182379/150‑1 (MQ=255)
   caagTTCGCGAACATGTTCTTCCGTTGCGTCCTCCTGAAAGGTGATTGTGGCACGGACTTTACAGTTAACCTGCAACACTACCAGCTTTGTGTCTTCCACCATCGCTTTTTCGTCAGCTAGCGGCCACGGGGCGTTGTCGATAtagcctt                                                                                                                                               <  2:672335/147‑1 (MQ=255)
    aCGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttc                                                                                                                                              <  1:1055831/150‑1 (MQ=255)
    aCGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttc                                                                                                                                              <  2:4958081/150‑1 (MQ=255)
    aCGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttc                                                                                                                                              <  1:2564985/150‑1 (MQ=255)
    aCGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttc                                                                                                                                              <  1:2389842/150‑1 (MQ=255)
      gTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttcgc                                                                                                                                            >  2:1793266/1‑150 (MQ=255)
        tCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttcgcct                                                                                                                                          <  1:3488984/150‑1 (MQ=255)
         cgcgAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATagccttcgcctt                                                                                                                                         <  2:2666948/150‑1 (MQ=255)
          gcgAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCttt                                                                                                                                        <  2:3902358/150‑1 (MQ=255)
           cgAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTc                                                                                                                                       <  2:4364456/150‑1 (MQ=255)
            gAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCa                                                                                                                                      <  1:4830906/150‑1 (MQ=255)
            gAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCa                                                                                                                                      >  1:3108281/1‑150 (MQ=255)
             aaCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAg                                                                                                                                     <  1:771028/150‑1 (MQ=255)
             aaCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTATACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTGTTTCGTCCGCACCCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTCTCAg                                                                                                                                     >  2:4265682/1‑150 (MQ=255)
               ccTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGtt                                                                                                                                   <  2:3832582/150‑1 (MQ=255)
                cTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTc                                                                                                                                  <  2:9228/150‑1 (MQ=255)
                  gTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCt                                                                                                                                >  1:2425230/1‑150 (MQ=255)
                  gTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCt                                                                                                                                >  2:4465060/1‑150 (MQ=255)
                     cttcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGcc                                                                                                                             <  2:4982033/150‑1 (MQ=255)
                         cGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAg                                                                                                                         >  2:3064832/1‑150 (MQ=255)
                            tGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGt                                                                                                                      >  1:3014994/1‑150 (MQ=255)
                               gTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGaa                                                                                                                   >  1:3714060/1‑150 (MQ=255)
                                tCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAg                                                                                                                  >  2:2758834/1‑150 (MQ=255)
                                 ccACCGGAACGGTGATGTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGc                                                                                                                 >  1:949722/1‑150 (MQ=255)
                                  cACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                                                <  1:2829914/150‑1 (MQ=255)
                                  cACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                                                >  2:3702476/1‑150 (MQ=255)
                                   aCCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                               <  1:5105300/150‑1 (MQ=255)
                                     cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAt                                                                                                             >  2:4109332/1‑150 (MQ=255)
                                      ggAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                            >  2:3541140/1‑150 (MQ=255)
                                      ggAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                            <  1:3981557/150‑1 (MQ=255)
                                      ggAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                            >  1:4277993/1‑150 (MQ=255)
                                       gAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgt                                                                                                           >  2:3396978/1‑150 (MQ=255)
                                       gAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgt                                                                                                           >  1:4364542/1‑150 (MQ=255)
                                          cGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCg                                                                                                        >  1:5292294/1‑150 (MQ=255)
                                           ggTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgg                                                                                                       <  1:3445305/150‑1 (MQ=255)
                                            gTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCATATGTGCggg                                                                                                      >  2:4556240/1‑150 (MQ=255)
                                            gTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                      >  2:2350339/1‑150 (MQ=255)
                                              gATTTTGGCACGGANTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTNCTGCCACAGCGTGAAGCAGATGTGCGGGGt                                                                                                    >  2:3604023/1‑150 (MQ=255)
                                              gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGt                                                                                                    <  1:691385/150‑1 (MQ=255)
                                              gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGt                                                                                                    <  1:1442677/150‑1 (MQ=255)
                                                  ttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAAc                                                                                                <  1:4595034/150‑1 (MQ=255)
                                                    ggCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACgg                                                                                              <  2:5147283/150‑1 (MQ=255)
                                                       aCGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGtt                                                                                           <  1:2889891/150‑1 (MQ=255)
                                                        cGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTa                                                                                          <  1:1967686/150‑1 (MQ=255)
                                                         ggACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTaa                                                                                         >  1:2083568/1‑150 (MQ=255)
                                                           aCTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGc                                                                                       <  2:3729210/150‑1 (MQ=255)
                                                           aCTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGc                                                                                       >  2:1112918/1‑150 (MQ=255)
                                                             tttACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCAt                                                                                     >  1:4464584/1‑150 (MQ=255)
                                                             tttACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCAt                                                                                     <  1:3759526/150‑1 (MQ=255)
                                                                aCCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACg                                                                                  <  1:4991394/150‑1 (MQ=255)
                                                                 ccGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACgg                                                                                 <  1:832783/150‑1 (MQ=255)
                                                                   gTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAc                                                                               <  2:2470710/150‑1 (MQ=255)
                                                                   gTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAc                                                                               >  2:2106423/1‑150 (MQ=255)
                                                                      aaCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAc                                                                            <  1:1492694/150‑1 (MQ=255)
                                                                      aaCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAc                                                                            <  2:1251564/150‑1 (MQ=255)
                                                                      aaCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCGTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAc                                                                            >  1:4688929/1‑150 (MQ=255)
                                                                       aCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACg                                                                           <  1:1341909/150‑1 (MQ=255)
                                                                        ccTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACgg                                                                          >  2:1685585/1‑150 (MQ=255)
                                                                           gCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCca                                                                       >  1:1548607/1‑150 (MQ=255)
                                                                             accacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcagc                                                                     >  1:2633678/1‑150 (MQ=255)
                                                                                 cGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTg                                                                 <  2:4708064/150‑1 (MQ=255)
                                                                                   aCCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCt                                                               <  1:3623785/150‑1 (MQ=255)
                                                                                    ccAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCtt                                                              >  1:878863/1‑150 (MQ=255)
                                                                                     cAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTc                                                             <  1:2203466/150‑1 (MQ=255)
                                                                                      aGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTcc                                                            <  2:2560993/150‑1 (MQ=255)
                                                                                        cGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTg                                                          >  1:2322307/1‑150 (MQ=255)
                                                                                         gTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGc                                                         >  2:2309959/1‑150 (MQ=255)
                                                                                          tGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCa                                                        <  1:2455334/150‑1 (MQ=255)
                                                                                          tGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCa                                                        <  1:2172436/150‑1 (MQ=255)
                                                                                            gAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATc                                                      <  1:4066177/150‑1 (MQ=255)
                                                                                              gTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCag                                                    >  2:2935116/1‑150 (MQ=255)
                                                                                                cTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTNCTGCATCagag                                                  <  1:2764525/150‑1 (MQ=255)
                                                                                                 ttCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAgc                                                 <  2:1925962/150‑1 (MQ=255)
                                                                                                  tCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAgcg                                                >  2:2221079/1‑150 (MQ=255)
                                                                                                      ccaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAt                                            <  1:4895097/150‑1 (MQ=255)
                                                                                                       caTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATc                                           <  2:3983863/150‑1 (MQ=255)
                                                                                                       caTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATc                                           >  2:510273/1‑150 (MQ=255)
                                                                                                         tCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCt                                         <  2:3124773/150‑1 (MQ=255)
                                                                                                         tCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCt                                         <  2:1563997/150‑1 (MQ=255)
                                                                                                             tttttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTc                                     <  2:1929765/150‑1 (MQ=255)
                                                                                                                 tCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCa                                 <  1:335206/150‑1 (MQ=255)
                                                                                                                   gTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATc                               >  1:962173/1‑150 (MQ=255)
                                                                                                                     cAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCgg                             >  1:4152936/1‑150 (MQ=255)
                                                                                                                        cAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTg                          <  2:5184541/150‑1 (MQ=255)
                                                                                                                          aCCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGt                        <  1:191553/150‑1 (MQ=255)
                                                                                                                          aCCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGt                        <  2:2839348/150‑1 (MQ=255)
                                                                                                                               ccACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCttt                   <  2:4834096/150‑1 (MQ=255)
                                                                                                                                   ggCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGcc               <  1:4770777/150‑1 (MQ=255)
                                                                                                                                          tGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGt        <  2:3485932/150‑1 (MQ=255)
                                                                                                                                             cGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGttaa     <  1:3541140/150‑3 (MQ=255)
                                                                                                                                              gATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcat    >  2:1616249/1‑150 (MQ=255)
                                                                                                                                               atatAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcatc   <  1:1729136/150‑1 (MQ=255)
                                                                                                                                               atatAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcatc   >  1:2959076/1‑150 (MQ=255)
                                                                                                                                               atatAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcatc   <  2:3307590/150‑1 (MQ=255)
                                                                                                                                               atatAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcatc   >  1:3891671/1‑150 (MQ=255)
                                                                                                                                                tataGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcatca  <  1:12954/150‑1 (MQ=255)
                                                                                                                                                tataGCCTTCGCCGTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCAGCCGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcatca  <  2:279067/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCA  >  CP009273/667752‑668045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑