Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 7,522 G→T 100% F146L (TTC→TTA)  yaaJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092737,5220GT99.3% 970.7 / ‑2.2 296F146L (TTC→TTA) yaaJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (157/137);  total (157/139)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 296 total aligned reads displayed.

GCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGG  >  CP009273/7375‑7667
                                                                                                                                                   |                                                                                                                                                 
  gAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACa                                                                                                                                               >  2:418445/1‑150 (MQ=255)
  gAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCGGTCACCAGCGGGGGAAAATCAAAAGAAAACCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCAGAGGCGATGAGCAAAAAGACGGCTAACa                                                                                                                                               <  2:4533734/150‑1 (MQ=255)
   aGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAg                                                                                                                                              <  2:2580696/150‑1 (MQ=255)
      gATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAAc                                                                                                                                           >  1:374403/1‑150 (MQ=255)
       aTTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACg                                                                                                                                          <  1:4273862/150‑1 (MQ=255)
       aTTGCCAGAAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACg                                                                                                                                          <  2:5047205/150‑1 (MQ=255)
         tGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGcc                                                                                                                                        >  1:4598531/1‑150 (MQ=255)
          gCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGccc                                                                                                                                       <  1:4745044/150‑1 (MQ=255)
            cagcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCAt                                                                                                                                     <  1:5081598/150‑1 (MQ=255)
             agcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATc                                                                                                                                    >  2:1468748/1‑150 (MQ=255)
             agcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATc                                                                                                                                    <  2:2957256/150‑1 (MQ=255)
              gcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATcc                                                                                                                                   <  2:2672890/150‑1 (MQ=255)
               cagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCa                                                                                                                                  <  2:3139266/150‑1 (MQ=255)
                agagTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAg                                                                                                                                 >  2:5178612/1‑150 (MQ=255)
                     aaaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCAt                                                                                                                            >  2:2093394/1‑150 (MQ=255)
                     aaaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCAt                                                                                                                            <  2:4590887/150‑1 (MQ=255)
                      aaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATc                                                                                                                           >  2:381155/1‑150 (MQ=255)
                       aGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAACAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATcc                                                                                                                          <  1:814749/150‑1 (MQ=255)
                       aGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATcc                                                                                                                          >  2:3241553/1‑150 (MQ=255)
                           aGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAg                                                                                                                      >  1:3025322/1‑150 (MQ=255)
                            gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGc                                                                                                                     >  1:4216343/1‑150 (MQ=255)
                              gagaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGccc                                                                                                                   <  1:1430957/150‑1 (MQ=255)
                               agaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCcg                                                                                                                  <  1:5206542/150‑1 (MQ=255)
                                gaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCcgc                                                                                                                 >  1:2562134/1‑150 (MQ=255)
                                  ataatGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgc                                                                                                               >  2:4304037/1‑150 (MQ=255)
                                       gCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCat                                                                                                          >  1:3178399/1‑150 (MQ=255)
                                         cTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCatat                                                                                                        >  1:2066470/1‑150 (MQ=255)
                                           gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAc                                                                                                      >  1:1542646/1‑150 (MQ=255)
                                           gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAc                                                                                                      >  2:378282/1‑150 (MQ=255)
                                            tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAcc                                                                                                     >  1:3279944/1‑150 (MQ=255)
                                             cACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCa                                                                                                    >  2:3956370/1‑150 (MQ=255)
                                             cACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCa                                                                                                    <  2:599947/150‑1 (MQ=255)
                                                 aGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGcc                                                                                                >  1:104987/1‑150 (MQ=255)
                                                     ggggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTc                                                                                            <  1:1375211/150‑1 (MQ=255)
                                                      gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTcc                                                                                           <  2:4159247/150‑1 (MQ=255)
                                                      gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTcc                                                                                           <  1:2794217/150‑1 (MQ=255)
                                                         aaaaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGcc                                                                                        >  2:2313734/1‑150 (MQ=255)
                                                          aaaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGccg                                                                                       <  1:502579/150‑2 (MQ=255)
                                                           aaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCAc                                                                                      <  1:4303170/150‑1 (MQ=255)
                                                            aTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACg                                                                                     <  2:2931113/150‑1 (MQ=255)
                                                            aTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACg                                                                                     <  1:1232748/150‑1 (MQ=255)
                                                               aaaaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                                  >  2:4612671/1‑150 (MQ=255)
                                                                aaaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAAc                                                                                 <  2:4872767/150‑1 (MQ=255)
                                                                 aaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACt                                                                                <  1:190702/150‑1 (MQ=255)
                                                                    aaaaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGcc                                                                             >  1:5290212/1‑150 (MQ=255)
                                                                    aaaaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGcc                                                                             >  2:2778312/1‑150 (MQ=255)
                                                                    aaaaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGcc                                                                             >  2:178662/1‑150 (MQ=255)
                                                                      aaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCa                                                                           <  2:3106379/150‑1 (MQ=255)
                                                                      aaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCa                                                                           <  1:3150432/150‑1 (MQ=255)
                                                                        aCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAtt                                                                         <  1:3937210/150‑1 (MQ=255)
                                                                        aCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAtt                                                                         >  2:2887679/1‑150 (MQ=255)
                                                                        aCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAtt                                                                         >  1:3323552/1‑150 (MQ=255)
                                                                         cTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTg                                                                        >  2:1849871/1‑150 (MQ=255)
                                                                           cAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGAc                                                                      <  1:3147891/150‑1 (MQ=255)
                                                                           cAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGAc                                                                      >  2:239550/1‑150 (MQ=255)
                                                                           cAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCACTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGAc                                                                      >  2:790981/1‑150 (MQ=255)
                                                                            aGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACg                                                                     <  1:284019/150‑1 (MQ=255)
                                                                                     gCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCt                                                            <  1:682689/150‑1 (MQ=255)
                                                                                       aaCGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCttt                                                          <  2:2034347/150‑1 (MQ=255)
                                                                                       aaCGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCttt                                                          >  2:5155823/1‑150 (MQ=255)
                                                                                           gCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATaa                                                      <  2:3566604/150‑1 (MQ=255)
                                                                                            cGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATaaa                                                     >  2:2741866/1‑150 (MQ=255)
                                                                                             gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                    >  2:1000277/1‑150 (MQ=255)
                                                                                             gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                    >  2:1690842/1‑150 (MQ=255)
                                                                                             gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                    <  1:2381594/150‑1 (MQ=255)
                                                                                             gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                    <  1:5239290/150‑1 (MQ=255)
                                                                                               tCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGtt                                                  <  1:215106/150‑1 (MQ=255)
                                                                                                cGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtg                                                 <  2:93276/150‑1 (MQ=255)
                                                                                                  cTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgtg                                               >  1:1365571/1‑150 (MQ=255)
                                                                                                   ttGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGc                                              >  2:3764960/1‑150 (MQ=255)
                                                                                                    tGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGcc                                             >  1:2751663/1‑150 (MQ=255)
                                                                                                      aaCTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCag                                           <  1:4197750/150‑1 (MQ=255)
                                                                                                       aCTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCaga                                          <  1:4915668/150‑1 (MQ=255)
                                                                                                             cTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAt                                    >  1:789809/1‑150 (MQ=255)
                                                                                                              tGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAtt                                   >  1:3586990/1‑150 (MQ=255)
                                                                                                              tGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAtt                                   <  1:5318593/150‑1 (MQ=255)
                                                                                                                  aaTTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGc                               >  2:1593955/1‑150 (MQ=255)
                                                                                                                   agtATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCa                              <  1:4666934/148‑1 (MQ=255)
                                                                                                                   aTTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCa                              >  2:3301959/1‑150 (MQ=255)
                                                                                                                   aTTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCa                              >  1:4138372/1‑150 (MQ=255)
                                                                                                                     tATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCaaa                            >  2:50006/1‑150 (MQ=255)
                                                                                                                      aTGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAAc                           <  2:5265145/150‑1 (MQ=255)
                                                                                                                         ccATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAg                        >  2:3511397/1‑150 (MQ=255)
                                                                                                                          cATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAgg                       <  1:3235450/150‑1 (MQ=255)
                                                                                                                           aTAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGt                      >  1:1743181/1‑150 (MQ=255)
                                                                                                                            tAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTc                     <  1:2189137/150‑1 (MQ=255)
                                                                                                                            tAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTc                     <  1:5143924/150‑1 (MQ=255)
                                                                                                                             aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCg                    >  2:4435752/1‑150 (MQ=255)
                                                                                                                             aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTGTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCg                    >  2:18237/1‑150 (MQ=255)
                                                                                                                                cGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCa                 <  1:3209746/150‑1 (MQ=255)
                                                                                                                                cGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCa                 >  2:1103855/1‑150 (MQ=255)
                                                                                                                                  aTGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATg               >  2:4963008/1‑150 (MQ=255)
                                                                                                                                  aTGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATg               >  1:1469973/1‑150 (MQ=255)
                                                                                                                                   tGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGc              >  1:3843968/1‑150 (MQ=255)
                                                                                                                                        aaaaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAGCTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATa         >  1:4018247/1‑150 (MQ=255)
                                                                                                                                        aaaaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATa         >  2:756407/1‑150 (MQ=255)
                                                                                                                                        aaaaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATa         >  2:3118090/1‑150 (MQ=255)
                                                                                                                                         aaaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaa        >  1:3506340/1‑150 (MQ=255)
                                                                                                                                          aaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaaa       <  2:5297480/150‑1 (MQ=255)
                                                                                                                                           aaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAc      <  2:900973/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGG  >  CP009273/7375‑7667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑