Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 723,859 G→T 100% V110V (GTC→GTA)  kdpA ← potassium translocating ATPase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273723,8590GT98.7% 987.7 / 2.5 307V110V (GTC→GTA) kdpApotassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (150/153);  total (151/156)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 307 total aligned reads displayed.

GCTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATA  >  CP009273/723712‑724005
                                                                                                                                                   |                                                                                                                                                  
 ctctGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACa                                                                                                                                                 <  1:1760281/150‑1 (MQ=255)
   ctGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACaaa                                                                                                                                               <  2:3254435/150‑1 (MQ=255)
       cGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTg                                                                                                                                           <  1:2882668/150‑1 (MQ=255)
         ggTAAACGCACGGATGAGGGCAAAAATCACCGCACTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAc                                                                                                                                         <  1:3405465/150‑1 (MQ=255)
         ggTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAc                                                                                                                                         <  2:3681370/150‑1 (MQ=255)
         ggTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAc                                                                                                                                         <  2:4761204/150‑1 (MQ=255)
         ggTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAc                                                                                                                                         >  1:73931/1‑150 (MQ=255)
          gTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcg                                                                                                                                        <  2:1309977/150‑1 (MQ=255)
          gTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcg                                                                                                                                        >  2:5082902/1‑150 (MQ=255)
          gTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcg                                                                                                                                        >  1:1864787/1‑150 (MQ=255)
          gTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcg                                                                                                                                        >  2:213553/1‑150 (MQ=255)
           tAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcgg                                                                                                                                       >  1:1624404/1‑150 (MQ=255)
            aaaCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcggc                                                                                                                                      <  2:3417329/150‑1 (MQ=255)
            aaaCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcggc                                                                                                                                      <  2:2421521/150‑1 (MQ=255)
             aaCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcggcg                                                                                                                                     >  1:4931442/1‑150 (MQ=255)
                   cGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTc                                                                                                                               >  2:2308183/1‑150 (MQ=255)
                    ggATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCa                                                                                                                              >  1:3084432/1‑150 (MQ=255)
                    ggATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCa                                                                                                                              <  2:5222477/150‑1 (MQ=255)
                    ggATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCa                                                                                                                              <  2:2085028/150‑1 (MQ=255)
                     gATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAg                                                                                                                             <  2:2547309/150‑1 (MQ=255)
                         aGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGcc                                                                                                                         <  2:2758711/150‑1 (MQ=255)
                                  aTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc                                                                                                                >  2:1160252/1‑150 (MQ=255)
                                   tCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGACATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACg                                                                                                               <  2:1539809/150‑1 (MQ=255)
                                          aaTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGccc                                                                                                        >  2:4145235/1‑150 (MQ=255)
                                          aaTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGccc                                                                                                        >  2:511495/1‑150 (MQ=255)
                                          aaTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGccc                                                                                                        >  2:794753/1‑150 (MQ=255)
                                           aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCt                                                                                                       <  1:2737155/150‑1 (MQ=255)
                                             cccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTgg                                                                                                     <  1:1470650/150‑1 (MQ=255)
                                                  tGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAAct                                                                                                <  2:589503/150‑1 (MQ=255)
                                                     cggcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgct                                                                                             <  2:2183030/150‑1 (MQ=255)
                                                        cAGAAAGTAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCATTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg                                                                                          <  2:1877101/150‑1 (MQ=255)
                                                        cAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg                                                                                          >  1:2741714/1‑150 (MQ=255)
                                                         agaaagaaAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                         >  1:1071432/1‑150 (MQ=255)
                                                          gaaagaaaGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGa                                                                                        >  1:1370574/1‑150 (MQ=255)
                                                           aaagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAt                                                                                       <  2:232511/150‑1 (MQ=255)
                                                            aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGCTCCCACGACAGCCCTGGCAACTGCTGTGGAtt                                                                                      >  1:1687527/1‑150 (MQ=255)
                                                            aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAtt                                                                                      <  2:2967804/150‑1 (MQ=255)
                                                            aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAtt                                                                                      <  1:4497264/150‑1 (MQ=255)
                                                             agaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                     <  1:4304181/150‑1 (MQ=255)
                                                             agaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                     <  1:2194546/150‑1 (MQ=255)
                                                                aagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGc                                                                                  <  1:1323552/150‑1 (MQ=255)
                                                                  gTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCgg                                                                                >  1:5122108/1‑150 (MQ=255)
                                                                    tttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCa                                                                              <  2:4202217/150‑1 (MQ=255)
                                                                     ttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGATATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTTTTGATTAAGCGGCAg                                                                             <  2:4611696/150‑1 (MQ=255)
                                                                       gcaactttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCACCTGCTGTGGATTAAGCGGCAGAt                                                                           >  2:2051004/7‑150 (MQ=255)
                                                                        cACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                          <  2:3271684/150‑1 (MQ=255)
                                                                        cACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                          <  1:2930929/150‑1 (MQ=255)
                                                                         aCCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAg                                                                         >  2:2874414/1‑150 (MQ=255)
                                                                          ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCACCTGCTGTGGATTAAGCGGCAGATAGt                                                                        >  1:1933867/1‑150 (MQ=255)
                                                                            gTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGc                                                                      >  1:5296449/1‑150 (MQ=255)
                                                                             ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCt                                                                     <  2:1107907/150‑1 (MQ=255)
                                                                                 gCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGTTTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGAcc                                                                 <  1:4177131/150‑1 (MQ=255)
                                                                                     gCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc                                                             >  2:4710434/1‑150 (MQ=255)
                                                                                     gCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc                                                             >  2:3314984/1‑150 (MQ=255)
                                                                                     gCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc                                                             <  2:4067163/150‑1 (MQ=255)
                                                                                         tCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACa                                                         <  2:4105496/150‑1 (MQ=255)
                                                                                         tCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACa                                                         <  1:558997/150‑1 (MQ=255)
                                                                                          cTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACAt                                                        >  2:5250469/1‑150 (MQ=255)
                                                                                           tGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATa                                                       <  2:4357292/150‑1 (MQ=255)
                                                                                              cTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaa                                                    <  2:1790973/150‑1 (MQ=255)
                                                                                              cTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaa                                                    <  2:198048/150‑1 (MQ=255)
                                                                                               tGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaaa                                                   >  1:1517276/1‑150 (MQ=255)
                                                                                               tGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaaa                                                   >  1:3385308/1‑150 (MQ=255)
                                                                                                 aaaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAAc                                                 >  2:4848101/1‑150 (MQ=255)
                                                                                                   aTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAg                                               >  1:76674/1‑150 (MQ=255)
                                                                                                   aTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAg                                               <  2:3095334/150‑1 (MQ=255)
                                                                                                    tAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGc                                              <  1:3803566/150‑1 (MQ=255)
                                                                                                     aGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCa                                             >  1:1829330/1‑150 (MQ=255)
                                                                                                      gCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCAc                                            <  1:1160252/150‑1 (MQ=255)
                                                                                                       cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCAcc                                           <  1:1668641/150‑1 (MQ=255)
                                                                                                         cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGc                                         <  1:2571146/150‑1 (MQ=255)
                                                                                                            cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAg                                      <  1:667811/150‑1 (MQ=255)
                                                                                                            cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAg                                      <  1:1410633/150‑1 (MQ=255)
                                                                                                            cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAg                                      >  1:2166123/1‑150 (MQ=255)
                                                                                                              ttttttCACCGATATAATATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCATATCCCACGACAGCCCTGGCAACTGCTGTTGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcc                                    <  1:3166473/147‑1 (MQ=255)
                                                                                                              tGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcc                                    <  2:1686594/150‑1 (MQ=255)
                                                                                                                 tttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCa                                 <  1:3302903/150‑1 (MQ=255)
                                                                                                                        gCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGtt                          >  1:2358312/1‑150 (MQ=255)
                                                                                                                        gCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGtt                          >  1:2102218/1‑150 (MQ=255)
                                                                                                                        gCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGtt                          >  1:347254/1‑150 (MQ=255)
                                                                                                                         cTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTc                         >  2:65363/1‑150 (MQ=255)
                                                                                                                          tataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                        >  1:4829235/1‑150 (MQ=255)
                                                                                                                           ataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAg                       >  2:1047208/1‑150 (MQ=255)
                                                                                                                              aGATTGCCAGTTGTTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGcc                    <  1:3918286/150‑1 (MQ=255)
                                                                                                                              aGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGcc                    <  1:4233386/150‑1 (MQ=255)
                                                                                                                               gATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCg                   >  1:4677344/1‑150 (MQ=255)
                                                                                                                                 ttGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCgag                 <  2:548433/150‑1 (MQ=255)
                                                                                                                                  tGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCgaga                >  1:4115971/1‑150 (MQ=255)
                                                                                                                                     cAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATg             <  2:2451895/150‑1 (MQ=255)
                                                                                                                                     cAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATg             <  1:3346704/150‑1 (MQ=255)
                                                                                                                                      aGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATgg            >  1:919424/1‑150 (MQ=255)
                                                                                                                                          ggTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcaca        <  1:1064840/150‑1 (MQ=255)
                                                                                                                                          ggTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcaca        >  2:135156/1‑150 (MQ=255)
                                                                                                                                          ggTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcaca        >  2:2701887/1‑150 (MQ=255)
                                                                                                                                          ggTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcaca        <  2:2739455/150‑1 (MQ=255)
                                                                                                                                            tATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACaaa      >  1:3027210/1‑150 (MQ=255)
                                                                                                                                             aTTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAg     <  2:4899290/150‑1 (MQ=255)
                                                                                                                                             aTTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAg     <  2:2016465/150‑1 (MQ=255)
                                                                                                                                               tGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGat   <  1:2969705/150‑1 (MQ=255)
                                                                                                                                                ggTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGata  >  1:251077/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATA  >  CP009273/723712‑724005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑