BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	732,494	G→T	100%	pseudogene (214/1137 nt)	ybfL →	pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	732,494	0	G	T	98.6%	1163.7 / 4.8	361	pseudogene (214/1137 nt)	ybfL	pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
Reads supporting (aligned to +/- strand): ref base G (4/1); new base T (179/177); total (183/178)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 365 total aligned reads displayed.

GAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGT  >  CP009273/732347‑732640

gagCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTAt                                                                                                                                                  <  2:4006590/150‑1 (MQ=255)

gagCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTAt                                                                                                                                                  <  2:4677371/150‑1 (MQ=255)

   cATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACt                                                                                                                                               >  1:3267776/1‑150 (MQ=255)

   cATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACt                                                                                                                                               >  1:1283290/1‑150 (MQ=255)

   cATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACt                                                                                                                                               <  2:5028155/150‑1 (MQ=255)

     tAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCactatactat                                                                                                                                             <  1:5122340/150‑1 (MQ=255)

               ggCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGtt                                                                                                                                   <  2:1203002/150‑1 (MQ=255)

                   tCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTAt                                                                                                                               <  2:4887762/150‑1 (MQ=255)

                    ccTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATc                                                                                                                              >  1:818225/1‑150 (MQ=255)

                     ctactaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATcc                                                                                                                             >  1:3608352/1‑150 (MQ=255)

                     ctactaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATcc                                                                                                                             <  2:4177520/150‑1 (MQ=255)

                      tactaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCt                                                                                                                            >  1:2150369/1‑150 (MQ=255)

                       actaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTg                                                                                                                           >  2:2929069/1‑150 (MQ=255)

                              aCTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGt                                                                                                                    >  1:477631/1‑150 (MQ=255)

                               cTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTc                                                                                                                   >  2:3022729/1‑150 (MQ=255)

                                         cGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAAtt                                                                                                         >  2:1983297/1‑150 (MQ=255)

                                           ttATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTc                                                                                                       >  2:2113825/1‑150 (MQ=255)

                                                 cTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGt                                                                                                 >  2:4574547/1‑150 (MQ=255)

                                                  tGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTg                                                                                                >  2:2725305/1‑150 (MQ=255)

                                                  tGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTg                                                                                                >  1:615446/1‑150 (MQ=255)

                                                    gTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCt                                                                                              <  2:2057538/150‑1 (MQ=255)

                                                     tGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCGGTATCAGTCCTGCAAAATTTCACGAGTGCtt                                                                                             >  2:3444830/1‑150 (MQ=255)

                                                        aGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttat                                                                                          >  2:3706055/1‑150 (MQ=255)

                                                        aGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttat                                                                                          >  2:630437/1‑150 (MQ=255)

                                                        aGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttat                                                                                          >  2:60521/1‑150 (MQ=255)

                                                        aGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttat                                                                                          <  1:2004654/150‑1 (MQ=255)

                                                           aaGTTGGGAAGATATAGAGGATTTCGGGGAAAAACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTaa                                                                                       <  1:3868949/150‑1 (MQ=255)

                                                            aGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAAc                                                                                      <  1:5195789/150‑1 (MQ=38)

                                                               tGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTgg                                                                                   <  2:1256894/150‑1 (MQ=37)

                                                               tGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTgg                                                                                   >  2:3329348/1‑150 (MQ=37)

                                                               tGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTgg                                                                                   <  1:1121505/150‑1 (MQ=37)

                                                                gggAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGCATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGa                                                                                  >  1:4033837/1‑150 (MQ=37)

                                                                 ggAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGAt                                                                                 <  1:2845612/150‑1 (MQ=37)

                                                                    aGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGGAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCg                                                                              <  1:1755898/150‑1 (MQ=255)

                                                                    aGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCg                                                                              <  1:3619052/150‑1 (MQ=37)

                                                                      atatAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTg                                                                            >  1:4546744/1‑150 (MQ=37)

                                                                      atatAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTg                                                                            >  1:3011570/1‑150 (MQ=37)

                                                                        ataGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAt                                                                          <  2:3542185/150‑1 (MQ=38)

                                                                        ataGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAt                                                                          <  2:2902684/150‑1 (MQ=38)

                                                                          agagGATTTCTGGGAAACACATCTAGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTg                                                                        <  1:1734166/150‑1 (MQ=255)

                                                                          agagGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTg                                                                        >  2:1110914/1‑150 (MQ=255)

                                                                            agGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGcc                                                                      <  2:1916788/150‑1 (MQ=255)

                                                                              gATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCAt                                                                    <  2:5106340/150‑1 (MQ=255)

                                                                              gATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCAt                                                                    <  2:4942026/150‑1 (MQ=255)

                                                                               aTTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCAtt                                                                   >  1:1794055/1‑150 (MQ=255)

                                                                                   cGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcatc                                                               >  1:2396377/1‑150 (MQ=255)

                                                                                   cGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcatc                                                               <  2:4244967/150‑1 (MQ=255)

                                                                                    ggggAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcatca                                                              >  1:2649219/1‑150 (MQ=255)

                                                                                       gAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAAt                                                           >  1:4658655/1‑150 (MQ=255)

                                                                                       gAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAAt                                                           <  1:926811/150‑1 (MQ=255)

                                                                                       gAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAAt                                                           <  1:154411/150‑1 (MQ=255)

                                                                                        aaaCACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATg                                                          <  2:832891/150‑1 (MQ=255)

                                                                                        aaaCACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATg                                                          <  1:3095993/150‑1 (MQ=255)

                                                                                         aaCACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGa                                                         >  1:2955346/1‑150 (MQ=255)

                                                                                         aaCACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGa                                                         >  1:1216599/1‑150 (MQ=255)

                                                                                              aTCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAg                                                    >  2:2412393/1‑150 (MQ=255)

                                                                                                ctcGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGAc                                                  <  1:4315323/150‑1 (MQ=255)

                                                                                                ctcGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGCGATTGCCATTCATCAAATGATAAAGAc                                                  <  1:741862/150‑1 (MQ=255)

                                                                                                 tcGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACg                                                 <  1:3413246/150‑1 (MQ=255)

                                                                                                   gATATCATGAAGCAATATGGTGATTTTGAAAATGGAATTCCTGATCACTAAACTATTGCAAGAGATGTATCCTGTATCAGTCCTGCAAAAATTCACGAGTGCTTTATTAACTGGATGCGAGAATGCCATTCATCAAATGATAAACACGTc                                               >  2:1772875/1‑150 (MQ=11)

                                                                                                    aTTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCa                                              >  2:4230346/1‑150 (MQ=255)

                                                                                                      ttCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCAtt                                            <  2:1847406/150‑1 (MQ=255)

                                                                                                      ttCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCAtt                                            <  1:2615207/150‑1 (MQ=255)

                                                                                                       tCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTg                                           <  2:155746/150‑1 (MQ=255)

                                                                                                        cTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGc                                          >  1:1571200/1‑150 (MQ=40)

                                                                                                           gAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAt                                       >  1:4433963/1‑150 (MQ=255)

                                                                                                             aGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTg                                     >  2:2826787/1‑150 (MQ=255)

                                                                                                             aGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTg                                     >  2:3897297/1‑150 (MQ=255)

                                                                                                               cAATATTGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGAt                                   <  2:3105659/150‑1 (MQ=255)

                                                                                                               cAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGAt                                   >  1:3574394/1‑150 (MQ=255)

                                                                                                               cAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCAGTGCAATTGAt                                   <  2:1579973/150‑1 (MQ=255)

                                                                                                                aaTATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATg                                  >  2:317798/1‑150 (MQ=255)

                                                                                                                aaTATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATg                                  <  2:1270332/150‑1 (MQ=255)

                                                                                                                 atatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATgg                                 >  2:2456077/1‑150 (MQ=255)

                                                                                                                 atatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATgg                                 <  1:670299/150‑1 (MQ=255)

                                                                                                                   atGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaa                               >  2:257606/1‑150 (MQ=255)

                                                                                                                    tGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaa                              >  1:1878900/1‑150 (MQ=255)

                                                                                                                     ggTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaaa                             >  1:4987239/1‑150 (MQ=255)

                                                                                                                       tGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAc                           <  1:2336000/150‑1 (MQ=255)

                                                                                                                       tGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAc                           <  1:507657/150‑1 (MQ=255)

                                                                                                                            ttGAAAATTGTATTCCTGTTCACTATACTATTGCAAGAGTTGTATCCTGTATAAGTCCTGCAAAATTTCACGAGTGCATTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTcc                      <  1:1446127/150‑1 (MQ=14)

                                                                                                                             tGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCg                     <  1:1043665/150‑1 (MQ=255)

                                                                                                                                aaaTGGTATTCNTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCa                  >  1:2762534/1‑150 (MQ=255)

                                                                                                                                 aaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAc                 >  2:5275301/1‑150 (MQ=255)

                                                                                                                                 aaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAc                 >  1:3798071/1‑150 (MQ=255)

                                                                                                                                  aTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAct                >  2:4503856/1‑150 (MQ=255)

                                                                                                                                    ggTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCActct              <  2:4772666/150‑1 (MQ=255)

                                                                                                                                    ggTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCActct              <  2:3142937/150‑1 (MQ=255)

                                                                                                                                    ggTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCActct              >  2:5091895/1‑150 (MQ=255)

                                                                                                                                      tATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTa            >  1:3218106/1‑150 (MQ=255)

                                                                                                                                      tATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTa            <  2:2132622/150‑1 (MQ=255)

                                                                                                                                       aTTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTAt           >  1:4247680/1‑150 (MQ=255)

                                                                                                                                        ttCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATg          >  1:3151781/1‑150 (MQ=255)

                                                                                                                                         tCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGa         >  1:1024129/1‑150 (MQ=255)

                                                                                                                                           cTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACa       >  2:3795925/1‑150 (MQ=255)

                                                                                                                                             gTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAag     >  2:3429600/1‑150 (MQ=255)

                                                                                                                                              ttCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAaga    <  2:2279313/150‑1 (MQ=255)

                                                                                                                                              ttCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAaga    >  1:5287386/1‑150 (MQ=255)

                                                                                                                                                cACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGt  >  2:1889728/1‑150 (MQ=255)

                                                                                                                                                cACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGACATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGt  >  1:3913674/1‑150 (MQ=255)

GAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGT  >  CP009273/732347‑732640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`