Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 784,094 G→T 100% A67E (GCG→GAG)  galM ← aldose 1‑epimerase; type‑1 mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273784,0940GT99.3% 1043.3 / ‑2.4 318A67E (GCG→GAG) galMaldose 1‑epimerase; type‑1 mutarotase
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (182/132);  total (186/132)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 318 total aligned reads displayed.

GCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGT  >  CP009273/783948‑784239
                                                                                                                                                  |                                                                                                                                                 
  cAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcc                                                                                                                                              <  2:1222312/150‑1 (MQ=255)
   aGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcct                                                                                                                                             >  2:713195/1‑150 (MQ=255)
    gCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctg                                                                                                                                            >  2:1082570/1‑150 (MQ=255)
      gACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgat                                                                                                                                          >  2:382277/1‑150 (MQ=255)
      gACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgat                                                                                                                                          >  2:3615428/1‑150 (MQ=255)
      gACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgat                                                                                                                                          >  2:4503295/1‑150 (MQ=255)
      gACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgat                                                                                                                                          <  2:3268221/150‑1 (MQ=255)
       aCGTTTGTCTAACAATTCCGGCCCGCCGTGCAGCTGTTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATATCGGCTATTGTCGATACGGTTGGCATAACGACCAATTGATGCACCCAGAAACTCGGcctgatc                                                                                                                                         <  1:100546/150‑1 (MQ=255)
         gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcct                                                                                                                                       <  2:4276127/150‑1 (MQ=255)
         gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcct                                                                                                                                       <  1:786697/150‑1 (MQ=255)
         gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcct                                                                                                                                       <  2:2328729/150‑1 (MQ=255)
         gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcct                                                                                                                                       <  2:5059696/150‑1 (MQ=255)
             gTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATACCGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATa                                                                                                                                   >  2:131273/1‑150 (MQ=255)
             gTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATa                                                                                                                                   >  2:183477/1‑150 (MQ=255)
               cGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGACAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTCTTGGCGATCCGGTTGGCATAACGACCAATAGAGGCCCCCCGAAACTCGGCCTGATCCTGATAGc                                                                                                                                 >  2:2375431/1‑150 (MQ=255)
                gAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCa                                                                                                                                >  1:3622527/1‑150 (MQ=255)
                  aCCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                              >  1:4066211/1‑150 (MQ=255)
                   cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTc                                                                                                                             <  2:255484/150‑1 (MQ=255)
                    ccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTcc                                                                                                                            <  2:5137466/150‑1 (MQ=255)
                     cTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCg                                                                                                                           <  1:1478550/150‑1 (MQ=255)
                              cgccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGcac                                                                                                                  >  1:4908284/1‑150 (MQ=255)
                               gccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGcaca                                                                                                                 >  1:1110049/1‑150 (MQ=255)
                               gccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGcaca                                                                                                                 <  2:3098889/150‑1 (MQ=255)
                                ccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAg                                                                                                                <  2:3364346/150‑1 (MQ=255)
                                 cgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGc                                                                                                               >  1:2170703/1‑150 (MQ=255)
                                 cgTGCAGCAGGTAAACGCCCAGACTAGGCGAAAGAGTCACGGATTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGAATTCCGGTCTGGCACAGc                                                                                                               >  1:570400/1‑150 (MQ=255)
                                   tGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCg                                                                                                             >  2:62783/1‑150 (MQ=255)
                                    gCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGa                                                                                                            <  2:4603992/150‑1 (MQ=255)
                                       gCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagca                                                                                                         <  2:1468270/150‑1 (MQ=255)
                                            ttAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGcc                                                                                                    <  2:5302814/150‑1 (MQ=255)
                                             tAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCt                                                                                                   >  2:4716222/1‑150 (MQ=255)
                                             tAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGAGACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCt                                                                                                   <  2:2023248/150‑1 (MQ=255)
                                               aCGCCCTGACTTGGCGAAAGCGTCACTGTTTCACCGTCAAAGGTATAAAGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGTCACAGCCGAGCATCGCCTcg                                                                                                 >  1:271002/1‑150 (MQ=255)
                                                 gCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAATGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCAGGGCTGGCACAGCCGAGCAGCGCCTcgcg                                                                                               >  1:4394216/1‑150 (MQ=255)
                                                 gCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTcgcg                                                                                               <  1:1766976/150‑1 (MQ=255)
                                                  cccTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCgg                                                                                              >  1:1277589/1‑150 (MQ=255)
                                                    cTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGAc                                                                                            <  1:4113732/150‑1 (MQ=255)
                                                    cTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGAc                                                                                            <  1:3125421/150‑1 (MQ=255)
                                                     tGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCATTAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACg                                                                                           >  2:1033983/1‑150 (MQ=255)
                                                      gACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGACTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGc                                                                                          >  1:4265163/1‑150 (MQ=255)
                                                       aCTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCt                                                                                         >  2:2936082/1‑150 (MQ=255)
                                                           ggCGAAAGCGTNACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCa                                                                                     >  1:2761235/1‑150 (MQ=255)
                                                           ggCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCa                                                                                     <  2:4414982/150‑1 (MQ=255)
                                                            gCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCAt                                                                                    <  2:559308/150‑1 (MQ=255)
                                                            gCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCAt                                                                                    <  2:496195/150‑1 (MQ=255)
                                                              gAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCg                                                                                  >  2:153715/1‑150 (MQ=255)
                                                                   cGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAg                                                                             <  2:4066775/150‑1 (MQ=255)
                                                                   cGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAg                                                                             <  1:2811944/150‑1 (MQ=255)
                                                                    gTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGc                                                                            >  2:1948169/1‑150 (MQ=255)
                                                                     tCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCg                                                                           <  1:1195324/150‑1 (MQ=255)
                                                                     tCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCg                                                                           <  1:4551956/150‑1 (MQ=255)
                                                                        cGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCCTCGGAAAGCGGaa                                                                        >  1:451508/1‑150 (MQ=255)
                                                                             tCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACgg                                                                   <  1:382277/150‑1 (MQ=255)
                                                                              cACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACggg                                                                  <  2:4202279/150‑1 (MQ=255)
                                                                              cACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACggg                                                                  >  1:1576130/1‑150 (MQ=255)
                                                                               aCCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCCTCGGAAAGCGGAATACGGGc                                                                 >  2:2983017/1‑150 (MQ=255)
                                                                                 cGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCgg                                                               >  2:2442267/1‑150 (MQ=255)
                                                                                 cGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCgg                                                               <  2:4787316/150‑1 (MQ=255)
                                                                                 cGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCGGCCATCGGAAAGCGGAATACGGGCgg                                                               >  1:4021177/1‑150 (MQ=255)
                                                                                         gTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaa                                                       <  2:3213061/150‑1 (MQ=255)
                                                                                          tataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaag                                                      <  1:4704554/150‑1 (MQ=255)
                                                                                           ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaagt                                                     <  1:120612/150‑1 (MQ=255)
                                                                                            taACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTc                                                    <  2:2483556/150‑1 (MQ=255)
                                                                                                    aTTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCa                                            >  2:1884489/1‑150 (MQ=255)
                                                                                                      tGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGt                                          <  1:2562192/150‑1 (MQ=255)
                                                                                                         cGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCa                                       >  1:3287124/1‑150 (MQ=255)
                                                                                                          gATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCAt                                      >  1:5101449/1‑150 (MQ=255)
                                                                                                               ggTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCg                                 >  2:2828461/1‑150 (MQ=255)
                                                                                                               ggTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCg                                 >  1:3036446/1‑150 (MQ=255)
                                                                                                                gTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGt                                >  2:885346/1‑150 (MQ=255)
                                                                                                                gTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGt                                <  1:3703592/150‑1 (MQ=255)
                                                                                                                  tGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGa                              <  1:232878/150‑1 (MQ=255)
                                                                                                                  tGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGa                              >  1:2132378/1‑150 (MQ=255)
                                                                                                                     cATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTa                           <  1:5307358/150‑1 (MQ=255)
                                                                                                                      aTAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCATAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTAc                          >  1:4615885/1‑150 (MQ=255)
                                                                                                                      aTAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTAc                          <  1:1374523/150‑1 (MQ=255)
                                                                                                                        aaCGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCa                        >  2:3685812/1‑150 (MQ=255)
                                                                                                                        aaCGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCa                        >  1:2968892/1‑150 (MQ=255)
                                                                                                                        aaCGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGGCCATCAGCGTGACTACCa                        >  1:1942341/1‑150 (MQ=255)
                                                                                                                         aCGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCAt                       >  2:3510076/1‑150 (MQ=255)
                                                                                                                             ccAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCt                   <  1:2645920/150‑1 (MQ=255)
                                                                                                                              cAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTg                  >  2:3922920/1‑150 (MQ=255)
                                                                                                                              cAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTg                  >  2:1433930/1‑150 (MQ=255)
                                                                                                                               aaTAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGc                 >  1:858798/1‑150 (MQ=255)
                                                                                                                               aaTAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGc                 >  1:18603/1‑150 (MQ=255)
                                                                                                                                aTAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCg                >  2:1103187/1‑150 (MQ=255)
                                                                                                                                aTAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCg                <  1:2271765/150‑1 (MQ=255)
                                                                                                                                  agagGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgtt              >  2:2229710/1‑150 (MQ=255)
                                                                                                                                  agagGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgtt              >  2:4668283/1‑150 (MQ=255)
                                                                                                                                      gCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTa          <  1:2061610/150‑1 (MQ=255)
                                                                                                                                        ccccAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGACTACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTAACATCCCTGCGGTGTTACg        >  2:5202656/1‑150 (MQ=255)
                                                                                                                                         cccAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGc       >  1:4806706/1‑150 (MQ=255)
                                                                                                                                         cccAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGc       >  2:1988907/1‑150 (MQ=255)
                                                                                                                                           cAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCaa     <  2:4930534/150‑1 (MQ=255)
                                                                                                                                            aGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCCTCGGCAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGGTACGCaca    >  2:2750384/1‑148 (MQ=255)
                                                                                                                                            aGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCAGCGTTGTTACGCaaa    >  1:1457108/1‑150 (MQ=255)
                                                                                                                                             gAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAg   <  1:5211136/150‑1 (MQ=255)
                                                                                                                                              aaaCTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGt  >  2:1150957/1‑150 (MQ=255)
                                                                                                                                              aaaCTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGt  <  1:1739319/150‑1 (MQ=255)
                                                                                                                                              aaaCTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGt  >  1:1238628/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGT  >  CP009273/783948‑784239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑