Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 800,779 G→T 100% E607D (GAG→GAT)  ybhJ → putative hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273800,7790GT99.7% 1001.2 / ‑4.5 311E607D (GAG→GAT) ybhJputative hydratase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (143/166);  total (144/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 317 total aligned reads displayed.

CGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCT  >  CP009273/800632‑800925
                                                                                                                                                   |                                                                                                                                                  
cGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATgt                                                                                                                                                  >  1:409313/1‑150 (MQ=255)
 ggTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAtgtg                                                                                                                                                 >  2:1192658/1‑150 (MQ=255)
  gTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATgtgt                                                                                                                                                >  1:3364363/1‑150 (MQ=255)
    taaaCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGATTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                              <  1:1324945/149‑1 (MQ=255)
    gAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                              <  2:4136779/150‑1 (MQ=255)
    gAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                              <  2:2546259/150‑1 (MQ=255)
     aaaCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTg                                                                                                                                             <  2:1319340/150‑1 (MQ=255)
     aaaCCTCTTATTATCTTACAAATCCGTTTGGTCTGGCGGTGTTTACCCTGTCTCGCCGCTACCCGGGTTTTGTTCGCATAATAAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTg                                                                                                                                             <  1:906384/150‑1 (MQ=255)
      aaCCTCTTCTTATCTTTCAAATCCGTTTGGTCTGGCGGATTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgc                                                                                                                                            <  1:5187024/150‑1 (MQ=255)
        ccTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcgc                                                                                                                                          >  1:779943/1‑150 (MQ=255)
        ccTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcgc                                                                                                                                          >  2:3433353/1‑150 (MQ=255)
           cttattAACGTTCAAATCCGATTGGTCTGGCGGTGTTTCCCCTGTCTCGCCGCGATCCCGGTTATGTTAGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCa                                                                                                                                       <  2:4031624/150‑1 (MQ=255)
              cttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                    >  2:4055179/1‑150 (MQ=255)
                tATCGTTAAAATCCGATTGGTCGGGCGGAGTTTACCCGGTCTCGCCGCGATCCCGGTTTTGTTAGCATATTTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAg                                                                                                                                  <  1:1247841/150‑1 (MQ=255)
                 aTCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGc                                                                                                                                 >  1:4151673/1‑150 (MQ=255)
                   cGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                               >  1:2997380/1‑150 (MQ=255)
                    gTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGa                                                                                                                              >  1:987373/1‑150 (MQ=255)
                     ttCAAATCCGTTTGGTCTGGCGGTGTTTACCCTGTCTCGCCGCGATCCGGTTTATTTTACCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGAt                                                                                                                             <  1:4684840/150‑1 (MQ=255)
                     ttCAAATCCGATTGGTCTGGCGGTGTTTACCCTGTCTCGCCGCGATCCGGGTTTTGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGAt                                                                                                                             <  2:5226610/150‑1 (MQ=255)
                         aaTCCGATTGGTCTGGCGGATTTTACCCTGTCTCGCCGCTATCCCGGTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCt                                                                                                                         <  1:2633500/150‑1 (MQ=255)
                         aaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCt                                                                                                                         >  2:167076/1‑150 (MQ=255)
                         aaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCt                                                                                                                         >  1:174089/1‑150 (MQ=255)
                               aTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAg                                                                                                                   >  1:1007633/1‑150 (MQ=255)
                                     cTGGCGGTGTTTACCCTGTCTCGCCGCGATCCGGGTTTTGTTCGCATATGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCat                                                                                                             <  1:216951/150‑1 (MQ=255)
                                       ggggttgTTTACCCTGTCTCGCCGCGATCCGGTTTTTTTTAGCATAATAAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCatat                                                                                                           <  1:2804264/144‑1 (MQ=255)
                                       ggcgttgTTTACCCTTTAGCGCCGATAGCCCGTTTATTTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCatat                                                                                                           <  1:3648526/144‑1 (MQ=255)
                                       ggCGGTGTTTACCCTGTCTCGCCGCGATCCCGTTTTTGTTAGCAGAATTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCatat                                                                                                           <  1:4540732/150‑1 (MQ=255)
                                        gcgttttttACCCTGTCTCGCCGCGATCCCGTTTTTTTTAGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATAtt                                                                                                          <  1:3748562/144‑1 (MQ=255)
                                        gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATAtt                                                                                                          <  2:4439636/150‑1 (MQ=255)
                                        gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATAtt                                                                                                          <  2:749530/150‑1 (MQ=255)
                                        gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATAtt                                                                                                          >  2:1459517/1‑150 (MQ=255)
                                         cGGAGTTAACCCTTTCTCGCCGCGATCCCGGTTATGTTCGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTg                                                                                                         <  1:465569/150‑1 (MQ=255)
                                             ttttACCCTGTCGCGCCGCGATCCCGTTTTTGTTAGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATcc                                                                                                     <  1:1429301/149‑1 (MQ=255)
                                              tttACCCTGTCTCGACGCGATCACGTTTATTTTATCAGAATTAAAGAGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGAATATTGATCCg                                                                                                    <  2:648784/150‑1 (MQ=255)
                                                tACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAACAGATTGCTGGTCAGGAGCATATTGATCCGCt                                                                                                  <  2:4631251/150‑1 (MQ=255)
                                                 aCCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTg                                                                                                 <  2:2910333/150‑1 (MQ=255)
                                                 aCCCTGTCTCGCCGCGAGCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTg                                                                                                 <  2:336475/150‑1 (MQ=255)
                                                   ccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCa                                                                                               <  2:3887610/150‑1 (MQ=255)
                                                          cgccgcTATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaa                                                                                        <  1:5254250/150‑1 (MQ=255)
                                                                gATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgc                                                                                  >  1:2700478/1‑150 (MQ=255)
                                                                gATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgc                                                                                  >  2:4748193/1‑150 (MQ=255)
                                                                 aTCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgca                                                                                 <  2:446229/150‑1 (MQ=255)
                                                                      ggTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                                                                            >  1:2242112/1‑150 (MQ=255)
                                                                      ggTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                                                                            >  2:4236380/1‑150 (MQ=255)
                                                                       gTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATgg                                                                           >  2:2018809/1‑150 (MQ=255)
                                                                       gTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATgg                                                                           >  1:2581860/1‑150 (MQ=255)
                                                                        ttATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGt                                                                          <  2:493794/150‑1 (MQ=255)
                                                                        ttATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGt                                                                          >  1:4814295/1‑150 (MQ=255)
                                                                         tttGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTc                                                                         <  2:2294774/148‑1 (MQ=255)
                                                                           tGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa                                                                       >  1:5320048/1‑150 (MQ=255)
                                                                           tGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa                                                                       >  2:1723128/1‑150 (MQ=255)
                                                                            tttAGCAGAATAACAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGTCAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                      <  1:18960/149‑1 (MQ=255)
                                                                            gTTAGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                      <  1:652945/150‑1 (MQ=255)
                                                                            gTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                      >  2:1869425/1‑150 (MQ=255)
                                                                                 cAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGt                                                                 >  1:2896496/1‑150 (MQ=255)
                                                                                 cAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGt                                                                 >  2:831710/1‑150 (MQ=255)
                                                                                  ataaGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                                <  1:5053641/148‑1 (MQ=255)
                                                                                  aGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                                >  1:4972850/1‑150 (MQ=255)
                                                                                  aGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                                >  2:2622772/1‑150 (MQ=255)
                                                                                     aGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                             >  2:3077805/1‑150 (MQ=255)
                                                                                      gTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAAc                                                            <  2:2163744/150‑1 (MQ=255)
                                                                                        aaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCa                                                          >  1:4895142/1‑150 (MQ=255)
                                                                                          aGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAgg                                                        >  1:2453079/1‑150 (MQ=255)
                                                                                            cGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCg                                                      <  2:805584/150‑1 (MQ=255)
                                                                                             gACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGa                                                     >  2:1808758/1‑150 (MQ=255)
                                                                                               ctgctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATg                                                   >  2:3695132/1‑150 (MQ=255)
                                                                                                tgctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGTGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATgg                                                  >  2:248802/1‑150 (MQ=255)
                                                                                                 gctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGt                                                 <  2:932592/150‑1 (MQ=255)
                                                                                                        tGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTgcgc                                          >  1:76091/1‑150 (MQ=255)
                                                                                                        tGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTgcgc                                          >  2:3178358/1‑150 (MQ=255)
                                                                                                         ggAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGcgcg                                         <  2:4315467/150‑1 (MQ=255)
                                                                                                            aaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGa                                      <  2:2570051/150‑1 (MQ=255)
                                                                                                              aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAAc                                    >  2:717349/1‑150 (MQ=255)
                                                                                                              aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAAc                                    <  1:3276860/150‑1 (MQ=255)
                                                                                                              aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAAc                                    >  2:4280530/1‑150 (MQ=255)
                                                                                                               tCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACa                                   <  1:394044/150‑1 (MQ=255)
                                                                                                               tCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCAGTAAGCAGATTGCGGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACa                                   <  2:2439700/150‑1 (MQ=255)
                                                                                                                cAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAg                                  >  2:18470/1‑150 (MQ=255)
                                                                                                                   cGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAggcg                               <  1:1516007/150‑1 (MQ=255)
                                                                                                                       tGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGa                           >  1:176498/1‑150 (MQ=255)
                                                                                                                        ggCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAg                          >  1:4377109/1‑150 (MQ=255)
                                                                                                                          cGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCCGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCt                        <  1:5161715/150‑1 (MQ=255)
                                                                                                                          cGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCt                        <  2:3579867/150‑1 (MQ=255)
                                                                                                                           ggggAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTg                       >  2:3246153/1‑150 (MQ=255)
                                                                                                                             ggAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGcc                     >  1:2873901/1‑150 (MQ=255)
                                                                                                                              gAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCa                    <  2:221882/150‑1 (MQ=255)
                                                                                                                               aaTGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAg                   >  1:3635086/1‑150 (MQ=255)
                                                                                                                                aTGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGc                  <  1:1415804/150‑1 (MQ=255)
                                                                                                                                  gTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgt                >  2:4528894/1‑150 (MQ=255)
                                                                                                                                  gTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgt                >  2:4815072/1‑150 (MQ=255)
                                                                                                                                     aGCGAGCTGACAGATGTGTTTGCGAGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGACCAGGCGGCGAGCTGCCAGCGtgtg             >  2:5055394/1‑150 (MQ=255)
                                                                                                                                        gAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTTCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt          <  1:3077805/150‑1 (MQ=255)
                                                                                                                                         aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTg         <  2:2708028/150‑1 (MQ=255)
                                                                                                                                         aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTg         >  1:2834098/1‑150 (MQ=255)
                                                                                                                                         aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTg         >  1:3987073/1‑150 (MQ=255)
                                                                                                                                           cTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAGTGGc       >  2:3719859/1‑150 (MQ=255)
                                                                                                                                            tGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg      >  2:3486617/1‑150 (MQ=255)
                                                                                                                                             gACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCgg     >  1:1832237/1‑150 (MQ=255)
                                                                                                                                              aCAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGt    >  2:921943/1‑150 (MQ=255)
                                                                                                                                                agatGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCt  <  2:566953/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCT  >  CP009273/800632‑800925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑