Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 816,302 G→T 100% R18S (AGG→AGT)  ybhM → UPF0005 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273816,3020GT98.9% 1095.2 / 2.1 349R18S (AGG→AGT) ybhMUPF0005 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (207/138);  total (209/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 350 total aligned reads displayed.

TTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTAT  >  CP009273/816155‑816448
                                                                                                                                                   |                                                                                                                                                  
tttCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTat                                                                                                                                                  <  2:1749221/150‑1 (MQ=255)
 ttCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGtata                                                                                                                                                 >  2:3628033/1‑150 (MQ=255)
   cTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATAtt                                                                                                                                               >  2:4922409/1‑150 (MQ=255)
     gTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTaa                                                                                                                                             >  2:1980817/1‑150 (MQ=255)
      tATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTaaa                                                                                                                                            <  2:2558947/150‑1 (MQ=255)
       aTCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAAc                                                                                                                                           >  1:1614632/1‑150 (MQ=255)
              attGCAAAAGTATTTTGTATGCGTGGTTTTATATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTatat                                                                                                                                    <  2:3695413/148‑1 (MQ=255)
               aTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATg                                                                                                                                   >  1:2003921/1‑150 (MQ=255)
               aTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATg                                                                                                                                   >  2:3925587/1‑150 (MQ=255)
                tGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATgg                                                                                                                                  >  2:4814644/1‑150 (MQ=255)
                   aaaaGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTc                                                                                                                               >  1:4500044/1‑150 (MQ=255)
                      aGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCAtt                                                                                                                            >  2:3579604/1‑150 (MQ=255)
                          ttttGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCg                                                                                                                        >  2:4727829/1‑150 (MQ=255)
                               tATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGTATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTa                                                                                                                   >  1:4011492/1‑150 (MQ=255)
                               tATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGACAGTATATTAAACGGTATATGGCTCATTACCGCTTTa                                                                                                                   >  2:5069320/1‑150 (MQ=255)
                                 tGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAgg                                                                                                                 <  2:2425198/150‑1 (MQ=255)
                                 tGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAgg                                                                                                                 <  2:5265779/150‑1 (MQ=255)
                                    gTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGttt                                                                                                              <  1:1335310/150‑1 (MQ=255)
                                     tGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtg                                                                                                             >  1:5207752/1‑150 (MQ=255)
                                          ttAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGc                                                                                                        >  2:4672901/1‑150 (MQ=255)
                                            aaaTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCaa                                                                                                      >  1:4763373/1‑150 (MQ=255)
                                             aaTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAAc                                                                                                     >  1:109841/1‑150 (MQ=255)
                                              aTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAAcc                                                                                                    >  2:936907/1‑150 (MQ=255)
                                                  cttGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAg                                                                                                <  1:1891261/149‑1 (MQ=255)
                                                  aTTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAg                                                                                                >  1:3561177/1‑150 (MQ=255)
                                                  aTTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAg                                                                                                >  1:3305564/1‑150 (MQ=255)
                                                      aTGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACt                                                                                            >  1:790328/1‑150 (MQ=255)
                                                       tGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTa                                                                                           <  1:2387107/150‑1 (MQ=255)
                                                        ggTTAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAg                                                                                          >  2:1594243/1‑150 (MQ=255)
                                                        ggTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAg                                                                                          >  2:3124814/1‑150 (MQ=255)
                                                        ggTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAg                                                                                          <  1:3029962/150‑1 (MQ=255)
                                                         gTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGc                                                                                         >  1:2531475/1‑150 (MQ=255)
                                                           gcctattaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATATATTAGATTTTCAGCACGAGGCCAGTATATTAACCGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCt                                                                                       <  1:1509901/147‑1 (MQ=255)
                                                           gAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCt                                                                                       >  2:4335099/1‑150 (MQ=255)
                                                             atattaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTgg                                                                                     >  1:429340/1‑150 (MQ=255)
                                                             atattaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTgg                                                                                     >  1:5225289/1‑150 (MQ=255)
                                                               attaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTgggg                                                                                   <  1:5169553/150‑1 (MQ=255)
                                                               attaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTgggg                                                                                   >  2:5236223/1‑150 (MQ=255)
                                                                ttaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGa                                                                                  >  2:2008999/1‑150 (MQ=255)
                                                                   atattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCt                                                                               >  1:2098522/1‑150 (MQ=255)
                                                                   atattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCt                                                                               >  2:4003940/1‑150 (MQ=255)
                                                                      ttttttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAg                                                                            >  2:3868090/1‑150 (MQ=255)
                                                                         tttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGtat                                                                         <  2:5161491/150‑1 (MQ=255)
                                                                         tttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGtat                                                                         <  1:4418397/150‑1 (MQ=255)
                                                                           tCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTatat                                                                       <  1:1522919/150‑1 (MQ=255)
                                                                            cTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATc                                                                      <  2:1412741/150‑1 (MQ=255)
                                                                              aaGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGa                                                                    >  1:683088/1‑150 (MQ=255)
                                                                                ggATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGaaa                                                                  >  2:3475064/1‑150 (MQ=255)
                                                                                ggATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGaaa                                                                  <  1:2816871/150‑1 (MQ=255)
                                                                                ggATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGaaa                                                                  >  1:4942293/1‑150 (MQ=255)
                                                                                 gATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAAt                                                                 >  2:557951/1‑150 (MQ=255)
                                                                                  aTAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAAtt                                                                >  1:3214058/1‑150 (MQ=255)
                                                                                  aTAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAAtt                                                                <  1:473942/150‑1 (MQ=255)
                                                                                  aTAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAAtt                                                                <  1:3669019/150‑1 (MQ=255)
                                                                                     aCTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACg                                                             <  1:479190/150‑1 (MQ=255)
                                                                                       tAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGc                                                           >  1:3863031/1‑150 (MQ=255)
                                                                                         aaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCaa                                                         >  2:4483853/1‑150 (MQ=255)
                                                                                         aaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTGTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCaa                                                         >  2:292918/1‑150 (MQ=255)
                                                                                          aaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAAc                                                        >  1:116422/1‑150 (MQ=255)
                                                                                          aaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGGTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAAc                                                        >  2:1884410/1‑150 (MQ=255)
                                                                                           aGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAAcc                                                       <  2:1513230/150‑1 (MQ=255)
                                                                                              aTGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCaaa                                                    >  2:4225376/1‑150 (MQ=255)
                                                                                                  aaTCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATg                                                >  2:704243/1‑150 (MQ=255)
                                                                                                    tCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGAt                                              >  2:1146929/1‑150 (MQ=255)
                                                                                                     cATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGAtt                                             <  2:4111476/150‑1 (MQ=255)
                                                                                                     cATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGAtt                                             >  2:3914434/1‑150 (MQ=255)
                                                                                                     cATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGAtt                                             >  2:1021446/1‑150 (MQ=255)
                                                                                                      aTACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGATGCAAGTATATTAAACGGTATATGGCTCATTAACTCTTTAGGTTTGGTGGCAACCTCAGGACAAGCCTGGGGAGATAAGTATATCGAAATTACGGCAACCAAATATGAtta                                            >  1:4219094/1‑149 (MQ=255)
                                                                                                      aTACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTc                                            <  1:3602610/150‑1 (MQ=255)
                                                                                                      aTACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTc                                            >  1:4651703/1‑150 (MQ=255)
                                                                                                       tACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTca                                           <  1:626489/150‑1 (MQ=255)
                                                                                                       tACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTca                                           <  1:3630339/150‑1 (MQ=255)
                                                                                                        aCTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcac                                          >  2:1300909/1‑150 (MQ=255)
                                                                                                         cTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcacc                                         >  1:2922521/1‑150 (MQ=255)
                                                                                                          tCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcacca                                        <  1:4112931/150‑1 (MQ=255)
                                                                                                           cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGTAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                                       >  2:3234396/1‑150 (MQ=255)
                                                                                                               aaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAat                                   <  2:2047246/150‑1 (MQ=255)
                                                                                                               aaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAat                                   >  1:1932060/1‑150 (MQ=255)
                                                                                                                   aGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                               <  1:1698158/150‑1 (MQ=255)
                                                                                                                    gCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtatg                              <  1:4990526/150‑1 (MQ=255)
                                                                                                                          aaTTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCa                        >  2:1034399/1‑150 (MQ=255)
                                                                                                                          aaTTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCa                        >  1:955758/1‑150 (MQ=255)
                                                                                                                           aTTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCAt                       >  1:2247592/1‑150 (MQ=255)
                                                                                                                              aGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAgg                    >  1:2023767/1‑150 (MQ=255)
                                                                                                                               gATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAggg                   >  2:1170178/1‑150 (MQ=255)
                                                                                                                                aTTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGt                  <  2:3392541/150‑1 (MQ=255)
                                                                                                                                      aGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat            >  2:5310567/1‑150 (MQ=255)
                                                                                                                                      aGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat            >  1:2455827/1‑150 (MQ=255)
                                                                                                                                      aGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat            <  2:3269827/150‑1 (MQ=255)
                                                                                                                                       gCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattatt           <  1:1015422/150‑1 (MQ=255)
                                                                                                                                         aCGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTAc         <  2:579752/150‑1 (MQ=255)
                                                                                                                                         aCGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTAc         >  2:1823409/1‑150 (MQ=255)
                                                                                                                                          cGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACt        <  2:4849067/150‑1 (MQ=255)
                                                                                                                                          cGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACt        <  2:3762123/150‑1 (MQ=255)
                                                                                                                                          cGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACt        <  1:3406964/150‑1 (MQ=255)
                                                                                                                                           gAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACtt       >  1:5318572/1‑150 (MQ=255)
                                                                                                                                           gAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACtt       >  1:3795714/1‑150 (MQ=255)
                                                                                                                                             ggCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtg     >  2:2185824/1‑150 (MQ=255)
                                                                                                                                               ccAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtgta   >  1:3319346/1‑150 (MQ=255)
                                                                                                                                                cAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtgtat  >  2:1650960/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTAT  >  CP009273/816155‑816448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑