Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 820,503 C→A 100% T354T (ACG→ACT)  ybhR ← inner membrane putative ABC superfamily transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273820,5030CA99.3% 1026.2 / ‑2.3 314T354T (ACG→ACT) ybhRinner membrane putative ABC superfamily transporter permease
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (146/165);  total (148/166)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 314 total aligned reads displayed.

CTGTTGTCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTC  >  CP009273/820356‑820649
                                                                                                                                                   |                                                                                                                                                  
 tgttgtCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTg                                                                                                                                                 <  1:5014471/150‑1 (MQ=255)
 tgttgtCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTg                                                                                                                                                 <  2:1277629/150‑1 (MQ=255)
    tgtCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGcc                                                                                                                                              >  2:4661744/1‑150 (MQ=255)
    tgtCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGcc                                                                                                                                              >  1:4205544/1‑150 (MQ=255)
        ccGCGTTCTGGAAAACAACTTTTTGCTGGCACTTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTa                                                                                                                                          <  2:749741/150‑1 (MQ=255)
        ccGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTa                                                                                                                                          >  2:3678435/1‑150 (MQ=255)
         cgcgTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTAt                                                                                                                                         >  2:4680854/1‑150 (MQ=255)
          gcgTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATc                                                                                                                                        <  2:4151532/150‑1 (MQ=255)
           cgTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCa                                                                                                                                       >  2:5143590/1‑150 (MQ=255)
           cgTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCa                                                                                                                                       <  1:412525/150‑1 (MQ=255)
             ttCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCAcc                                                                                                                                     >  2:3939527/1‑150 (MQ=255)
              tCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCa                                                                                                                                    <  1:4919638/150‑1 (MQ=255)
              tCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCa                                                                                                                                    >  2:4567265/1‑150 (MQ=255)
               cTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAg                                                                                                                                   <  1:3419386/150‑1 (MQ=255)
                 ggAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTa                                                                                                                                 >  1:1139370/1‑150 (MQ=255)
                 ggAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTa                                                                                                                                 >  2:42102/1‑150 (MQ=255)
                  gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGACGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGtcg                                                                                                                                >  2:3130587/1‑148 (MQ=255)
                  gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAg                                                                                                                                >  2:1851961/1‑150 (MQ=255)
                   aaaaCAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGGTATCACCAGTAGc                                                                                                                               >  1:4516485/1‑150 (MQ=255)
                    aaaCAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCg                                                                                                                              <  2:2136224/150‑1 (MQ=255)
                     aacaacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCgg                                                                                                                             <  2:4776978/150‑1 (MQ=255)
                     aacaacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCgg                                                                                                                             >  1:4600740/1‑150 (MQ=255)
                     aacaacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCgg                                                                                                                             >  1:2508682/1‑150 (MQ=255)
                     aacaacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCgg                                                                                                                             <  2:1900232/150‑1 (MQ=255)
                         acTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCcac                                                                                                                         >  1:2978607/1‑150 (MQ=255)
                         acTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCcac                                                                                                                         <  2:159246/150‑1 (MQ=255)
                         acTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCcac                                                                                                                         <  2:683214/150‑1 (MQ=255)
                              gtGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACt                                                                                                                    <  2:4918281/149‑1 (MQ=255)
                                gCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTAt                                                                                                                  <  1:3474782/150‑1 (MQ=255)
                                gCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTAt                                                                                                                  <  2:2239596/150‑1 (MQ=255)
                                 cTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTAtt                                                                                                                 >  2:2103173/1‑150 (MQ=255)
                                     cAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCCGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcac                                                                                                             >  2:2718541/1‑150 (MQ=255)
                                      aaTTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCTCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcaca                                                                                                            >  1:1828125/1‑150 (MQ=255)
                                      aaTTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcaca                                                                                                            >  1:4146791/1‑150 (MQ=255)
                                          gCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAat                                                                                                        <  1:3841588/150‑1 (MQ=255)
                                              ggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATcc                                                                                                    <  1:2046753/150‑1 (MQ=255)
                                              ggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATcc                                                                                                    <  2:5035873/150‑1 (MQ=255)
                                               gCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGAGTAAGATTATAAGTTACATCTCCTTTCGTCTTATCATAGCGTACGCCGCTGACCGTGTAGTGGCCTTTATCACCTGTAGCGGCCACAAACTATTCCACACAATATCCa                                                                                                   <  1:1463310/150‑1 (MQ=255)
                                                 tGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGa                                                                                                 >  2:1442228/1‑150 (MQ=255)
                                                 tGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGa                                                                                                 <  1:432502/150‑1 (MQ=255)
                                                 tGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGa                                                                                                 >  2:613720/1‑150 (MQ=255)
                                                   gCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACt                                                                                               <  1:3139965/150‑1 (MQ=255)
                                                     aaTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCg                                                                                             >  1:4814820/1‑150 (MQ=255)
                                                     aaTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCg                                                                                             >  1:3596690/1‑150 (MQ=255)
                                                      aTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGc                                                                                            >  1:756779/1‑150 (MQ=255)
                                                        ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCAt                                                                                          <  2:449838/150‑1 (MQ=255)
                                                        ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCAt                                                                                          >  2:3828157/1‑150 (MQ=255)
                                                         cTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATc                                                                                         <  2:2398411/150‑1 (MQ=255)
                                                            gCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCtt                                                                                      <  1:1303148/150‑1 (MQ=255)
                                                                 cccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaat                                                                                 <  2:253216/150‑1 (MQ=255)
                                                                  ccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaata                                                                                >  1:1897065/1‑150 (MQ=255)
                                                                    ggcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTGCaaataaa                                                                              >  2:1660962/1‑150 (MQ=255)
                                                                     gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                             >  2:3254753/1‑150 (MQ=255)
                                                                      cggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaaac                                                                            >  1:842387/1‑148 (MQ=255)
                                                                       ggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCt                                                                           <  2:847423/150‑1 (MQ=255)
                                                                         tAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                         <  1:2121933/150‑1 (MQ=255)
                                                                          aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                        >  1:1050797/1‑150 (MQ=255)
                                                                          aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                        >  1:4708506/1‑150 (MQ=255)
                                                                          aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                        >  1:4417115/1‑150 (MQ=255)
                                                                           gtgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCtt                                                                       <  2:1946581/150‑1 (MQ=255)
                                                                                    cTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTc                                                              <  2:3996313/150‑1 (MQ=255)
                                                                                    cTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTc                                                              <  1:2254597/150‑1 (MQ=255)
                                                                                     tGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTcc                                                             >  2:2140787/1‑150 (MQ=255)
                                                                                       gCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGt                                                           <  2:1148632/150‑1 (MQ=255)
                                                                                        cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                          >  1:5071361/1‑150 (MQ=255)
                                                                                         gAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaa                                                         >  1:1996508/1‑150 (MQ=255)
                                                                                         gAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaa                                                         <  1:1595183/150‑1 (MQ=255)
                                                                                          aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                        >  1:858224/1‑150 (MQ=255)
                                                                                            aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGt                                                      >  1:3209471/1‑150 (MQ=255)
                                                                                            aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGt                                                      >  1:2721849/1‑150 (MQ=255)
                                                                                            aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGt                                                      >  2:2855690/1‑150 (MQ=255)
                                                                                             gATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                     >  1:2905964/1‑150 (MQ=255)
                                                                                              aTAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                    >  2:1305764/1‑150 (MQ=255)
                                                                                               tAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGc                                                   <  1:1220644/150‑1 (MQ=255)
                                                                                               tAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGc                                                   >  1:1001632/1‑150 (MQ=255)
                                                                                               tAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGc                                                   <  2:4343420/150‑1 (MQ=255)
                                                                                                 agaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGa                                                 <  2:1730916/150‑1 (MQ=255)
                                                                                                    agTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATa                                              >  1:713747/1‑150 (MQ=255)
                                                                                                    agTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATa                                              >  2:4845927/1‑150 (MQ=255)
                                                                                                     gTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAg                                             >  2:3871416/1‑150 (MQ=255)
                                                                                                         cATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGtt                                         <  2:2993537/150‑1 (MQ=255)
                                                                                                           tCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTaa                                       <  1:4550022/150‑1 (MQ=255)
                                                                                                            cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                      >  2:4949124/1‑150 (MQ=255)
                                                                                                              ccTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                    >  1:4114969/1‑150 (MQ=255)
                                                                                                               cTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCa                                   >  2:3444138/1‑150 (MQ=255)
                                                                                                                ttACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCAc                                  >  2:5246638/1‑150 (MQ=255)
                                                                                                                    gTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                              >  1:3540021/1‑150 (MQ=255)
                                                                                                                      cTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAgg                            >  2:1656538/1‑150 (MQ=255)
                                                                                                                      cTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAgg                            <  2:37696/150‑1 (MQ=255)
                                                                                                                       tAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGt                           >  1:4399322/1‑150 (MQ=255)
                                                                                                                           cATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTg                       >  1:3791946/1‑150 (MQ=255)
                                                                                                                            aTCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGc                      >  2:3605450/1‑150 (MQ=255)
                                                                                                                               gcgAACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGAGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGc                   >  1:174007/1‑150 (MQ=255)
                                                                                                                               gccaaCGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGGAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGc                   >  1:1578185/5‑150 (MQ=255)
                                                                                                                                    cgccgcTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATAc              <  1:3939527/150‑1 (MQ=255)
                                                                                                                                     gccgcTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATAcc             <  2:917730/150‑1 (MQ=255)
                                                                                                                                       cgcTTACCCTCTCGTGGCCGTTATCCCCCGTAGAGGCCACAAACTATTCCACACGATATCCAGACTCGCATCCTTCAATTAAATCTCCTTGGTAATGTCAGTAAAGTGGCGAATAGGGGTAATCCACGTCAGGTGTTGCAGCCATACCgg           >  2:653838/1‑150 (MQ=255)
                                                                                                                                       cgcTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTTCAGCCATACCgg           >  1:2789576/1‑150 (MQ=255)
                                                                                                                                          tGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCAt        <  1:5243094/150‑1 (MQ=255)
                                                                                                                                            aCCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt      <  1:4661744/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGTTGTCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTC  >  CP009273/820356‑820649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑