Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 876,395 G→T 100% M71I (ATG→ATT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273876,3950GT99.0% 976.3 / 0.1 306M71I (ATG→ATT) dacCD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (123/179);  total (126/180)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 306 total aligned reads displayed.

CCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC  >  CP009273/876248‑876541
                                                                                                                                                   |                                                                                                                                                  
cccAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTAc                                                                                                                                                  >  1:4362196/1‑150 (MQ=255)
  cAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCa                                                                                                                                                <  1:446440/150‑1 (MQ=255)
  cAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCa                                                                                                                                                >  1:1306974/1‑150 (MQ=255)
  cAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCa                                                                                                                                                >  1:474591/1‑150 (MQ=255)
   aaCGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAg                                                                                                                                               <  2:4229693/150‑1 (MQ=255)
      ggCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTa                                                                                                                                            <  2:336041/150‑1 (MQ=255)
         aTTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAtgt                                                                                                                                         <  1:1036440/150‑1 (MQ=255)
         aTTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAtgt                                                                                                                                         >  1:3323917/1‑150 (MQ=255)
         aTTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAtgt                                                                                                                                         >  2:4687707/1‑150 (MQ=255)
            cgcgGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGt                                                                                                                                      >  1:1676948/1‑150 (MQ=255)
                 cggAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGc                                                                                                                                 <  1:3556528/150‑1 (MQ=255)
                      cAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGgcg                                                                                                                            >  1:1216265/1‑150 (MQ=255)
                      cAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGgcg                                                                                                                            <  1:994886/150‑1 (MQ=255)
                        aaCCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCt                                                                                                                          <  1:5027549/150‑1 (MQ=255)
                        aaCCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATAACCAGCTATGTGGTTGGGCAGGCGCt                                                                                                                          >  1:1879070/1‑150 (MQ=255)
                         aCCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCtt                                                                                                                         <  1:2000538/150‑1 (MQ=255)
                            gTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAg                                                                                                                      <  2:4501276/150‑1 (MQ=255)
                            gTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAg                                                                                                                      <  2:1877907/150‑1 (MQ=255)
                            gTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAg                                                                                                                      <  1:4448455/150‑1 (MQ=255)
                              tGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGc                                                                                                                    <  1:3616333/150‑1 (MQ=255)
                               gAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGcc                                                                                                                   <  1:2889048/150‑1 (MQ=255)
                               gAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGcc                                                                                                                   >  1:4250572/1‑150 (MQ=255)
                               gAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGcc                                                                                                                   >  2:2378617/1‑150 (MQ=255)
                                   cgcCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATa                                                                                                               >  1:4141761/1‑150 (MQ=255)
                                       gcgCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGAt                                                                                                           <  2:5262909/148‑1 (MQ=255)
                                        aGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGAtt                                                                                                          <  2:2782694/150‑1 (MQ=255)
                                             ggATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACt                                                                                                     <  1:4792177/150‑1 (MQ=255)
                                             ggATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTGAAGGCCGATAAGATTAAACt                                                                                                     >  2:2900344/1‑150 (MQ=255)
                                                tGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCAc                                                                                                  <  1:471436/150‑1 (MQ=255)
                                                tGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCAc                                                                                                  <  1:4072974/150‑1 (MQ=255)
                                                   gcgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCAccaa                                                                                               >  1:14784/1‑148 (MQ=255)
                                                    cgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAat                                                                                              >  2:1410094/1‑150 (MQ=255)
                                                     gTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGAGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAata                                                                                             >  2:1012667/1‑150 (MQ=255)
                                                      tGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAatat                                                                                            <  2:3642429/150‑1 (MQ=255)
                                                       gCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATg                                                                                           <  1:2920883/150‑1 (MQ=255)
                                                        cATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATgg                                                                                          >  1:1245526/1‑150 (MQ=255)
                                                         aTGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGt                                                                                         >  2:2651281/1‑150 (MQ=255)
                                                          tGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTg                                                                                        <  1:746002/150‑1 (MQ=255)
                                                           ggATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGa                                                                                       <  2:2123481/150‑1 (MQ=255)
                                                           ggATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGa                                                                                       >  2:4794449/1‑150 (MQ=255)
                                                            gATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAc                                                                                      <  2:3215851/150‑1 (MQ=255)
                                                            gATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAc                                                                                      >  2:1495981/1‑150 (MQ=255)
                                                            gATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAc                                                                                      <  2:4514331/150‑1 (MQ=255)
                                                             aTTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcg                                                                                     >  2:136390/1‑150 (MQ=255)
                                                                ttAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtc                                                                                  <  1:3080836/150‑1 (MQ=255)
                                                                 tAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcg                                                                                 <  1:845747/150‑1 (MQ=255)
                                                                  aaTGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcgg                                                                                <  1:3161493/150‑1 (MQ=255)
                                                                   aTGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcggt                                                                               >  2:4736102/1‑150 (MQ=255)
                                                                    tGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTa                                                                              <  1:2299039/150‑1 (MQ=255)
                                                                         tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGAt                                                                         <  1:4912030/150‑1 (MQ=255)
                                                                         tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGAt                                                                         >  1:2122188/1‑150 (MQ=255)
                                                                            gCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGcc                                                                      >  2:628597/1‑150 (MQ=255)
                                                                            gCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGcc                                                                      <  1:863732/150‑1 (MQ=255)
                                                                            gCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGcc                                                                      <  2:234619/150‑1 (MQ=255)
                                                                             ccAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCt                                                                     >  2:3011919/1‑150 (MQ=255)
                                                                               aGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCACTATGGTGACGGTCGGTAAAGATGCCTgg                                                                   >  2:4464726/1‑150 (MQ=255)
                                                                                 cGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGc                                                                 <  1:5087037/150‑1 (MQ=255)
                                                                                  ggTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCg                                                                >  1:1794271/1‑150 (MQ=255)
                                                                                    tAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGAc                                                              >  2:2269495/1‑150 (MQ=255)
                                                                                     aaaGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACg                                                             <  1:4579874/150‑1 (MQ=255)
                                                                                         tGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGaa                                                         <  2:3920596/150‑1 (MQ=255)
                                                                                           cTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAt                                                       >  1:2602642/1‑150 (MQ=255)
                                                                                            tGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATc                                                      <  2:597565/150‑1 (MQ=255)
                                                                                               cAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCgg                                                   >  2:880368/1‑150 (MQ=255)
                                                                                               cAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCGATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTCAAGATGCCTGGGCGACGGGACATCCgg                                                   >  1:2840356/1‑150 (MQ=255)
                                                                                                aGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGc                                                  <  1:1189972/150‑1 (MQ=255)
                                                                                                 gAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCa                                                 >  1:3709268/1‑150 (MQ=255)
                                                                                                  aaGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCAc                                                >  2:3860849/1‑150 (MQ=255)
                                                                                                   aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACt                                               >  1:2674038/1‑150 (MQ=255)
                                                                                                   aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACt                                               <  1:3369146/150‑1 (MQ=255)
                                                                                                   aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACt                                               <  2:429247/150‑1 (MQ=255)
                                                                                                    ggCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTg                                              >  1:5153189/1‑150 (MQ=255)
                                                                                                       aaTGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGAGAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGt                                           >  2:1941873/1‑150 (MQ=255)
                                                                                                        aTGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTg                                          <  1:519250/150‑1 (MQ=255)
                                                                                                         tGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTgg                                         <  1:2441809/150‑1 (MQ=255)
                                                                                                           cGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGtt                                       <  1:3476628/150‑1 (MQ=255)
                                                                                                           cGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGtt                                       <  2:827774/150‑1 (MQ=255)
                                                                                                             gATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCa                                     >  1:4666115/1‑150 (MQ=255)
                                                                                                              aTGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCAt                                    >  2:347015/1‑150 (MQ=255)
                                                                                                                     aCTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAt                             <  2:3101061/150‑1 (MQ=255)
                                                                                                                      cTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATg                            <  1:1581109/150‑1 (MQ=255)
                                                                                                                      cTGGATACCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATg                            <  1:3598621/150‑1 (MQ=255)
                                                                                                                       tGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGt                           >  1:3758778/1‑150 (MQ=255)
                                                                                                                        ggATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGtt                          >  1:3264426/1‑150 (MQ=255)
                                                                                                                           tCCCGCGAGCCTGACTAAAATCATTTCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGTGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCt                       <  2:499433/150‑1 (MQ=255)
                                                                                                                           tCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCt                       <  1:599074/150‑1 (MQ=255)
                                                                                                                           tCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCt                       <  2:1465791/150‑1 (MQ=255)
                                                                                                                            cccGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTc                      >  1:3898163/1‑150 (MQ=255)
                                                                                                                              cgcgAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaa                    >  1:538939/1‑150 (MQ=255)
                                                                                                                               gcgAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaaa                   <  2:2288972/150‑1 (MQ=255)
                                                                                                                                 gAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                 <  2:1623977/150‑1 (MQ=255)
                                                                                                                                  aGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCg                >  2:3807166/1‑150 (MQ=255)
                                                                                                                                   gCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCgg               >  1:1444203/1‑150 (MQ=255)
                                                                                                                                    ccTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCggg              <  1:2065442/150‑1 (MQ=255)
                                                                                                                                      tGACTAAAATCATTACCAGCTATGTGGTTGGTCAGGCGCTTAAGGCCAATAAGATTAAACTCAACAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCg            >  1:4292757/1‑150 (MQ=255)
                                                                                                                                      tGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCg            >  2:3999962/1‑150 (MQ=255)
                                                                                                                                        aCTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGAt          >  2:1157143/1‑150 (MQ=255)
                                                                                                                                          tAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCa        <  2:5045507/150‑1 (MQ=255)
                                                                                                                                              aTCATTACCAGCTATGTGGTTGGGCAGGCGATTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGtt    >  2:1559845/1‑150 (MQ=255)
                                                                                                                                                cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc  <  2:4141761/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC  >  CP009273/876248‑876541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑