Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 882,672 G→T 100% L42I (CTC→ATC)  ybjJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273882,6720GT99.8% 971.6 / ‑6.0 304L42I (CTC→ATC) ybjJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (163/140);  total (164/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 317 total aligned reads displayed.

TCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAG  >  CP009273/882525‑882818
                                                                                                                                                   |                                                                                                                                                  
tcCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATaa                                                                                                                                                  <  2:4404108/150‑1 (MQ=255)
 cccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAat                                                                                                                                                 >  1:2248900/1‑150 (MQ=255)
  ccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGataata                                                                                                                                                <  2:2766135/150‑1 (MQ=255)
   cGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAatat                                                                                                                                               <  1:4970510/150‑1 (MQ=255)
   cGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAatat                                                                                                                                               <  2:502660/150‑1 (MQ=255)
    gATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATc                                                                                                                                              >  1:2135776/1‑150 (MQ=255)
    gATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATc                                                                                                                                              >  1:1491998/1‑150 (MQ=255)
     aTCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcg                                                                                                                                             >  1:3228863/1‑150 (MQ=255)
     aTCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGACCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCCCCCATGTCAGCGATCGAGACAGAGATAATATcg                                                                                                                                             >  2:3554494/1‑150 (MQ=255)
       cAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcgcg                                                                                                                                           >  1:1158694/1‑150 (MQ=255)
        aaTGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCgg                                                                                                                                          >  2:895781/1‑150 (MQ=255)
          tGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGAt                                                                                                                                        <  1:4359884/150‑1 (MQ=255)
             gcAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGc                                                                                                                                     >  2:152957/1‑150 (MQ=255)
              cAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCa                                                                                                                                    <  2:644523/150‑1 (MQ=255)
               aGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAg                                                                                                                                   <  1:574793/150‑1 (MQ=255)
                       gTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACgg                                                                                                                           >  2:4080524/1‑150 (MQ=255)
                        tGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACggg                                                                                                                          >  2:1857884/1‑150 (MQ=255)
                          aCCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGtt                                                                                                                        >  1:1624067/1‑150 (MQ=255)
                          aCCAGGATGACATTACGTGACCCAAAGCGTTTCACTAACCACGCCGACCAGAGAATACCGCTCATCGAACAGATCGACAGACCAAAGAGAACACCGCCCATTTCATCGATCGAGACAGATATAATATCGAGGATAGCAGGCGTACGGGtt                                                                                                                        >  1:3000896/1‑150 (MQ=255)
                           ccAGGATGACATTACGTGTCCCAAAGCGTTTAACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTg                                                                                                                       >  1:2660001/1‑150 (MQ=255)
                             aGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGcc                                                                                                                     <  1:2885167/150‑1 (MQ=255)
                              ggATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGccc                                                                                                                    >  1:1727407/1‑150 (MQ=255)
                                aTGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAg                                                                                                                  >  2:4856808/1‑150 (MQ=255)
                                     aTTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGc                                                                                                             >  1:4670074/1‑150 (MQ=255)
                                               ccAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAgg                                                                                                   >  1:4317192/1‑150 (MQ=255)
                                                 aaaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                 <  1:2588682/150‑1 (MQ=255)
                                                   aGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTg                                                                                               >  2:2517028/1‑150 (MQ=255)
                                                    gCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTgg                                                                                              <  1:4150626/150‑1 (MQ=255)
                                                      gTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCa                                                                                            >  1:3073332/1‑150 (MQ=255)
                                                      gTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCa                                                                                            >  1:2198851/1‑150 (MQ=255)
                                                           aCTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaag                                                                                       <  1:3323597/150‑1 (MQ=255)
                                                            cTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaaga                                                                                      <  2:3285923/150‑1 (MQ=255)
                                                               aCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAagaaga                                                                                   >  1:950430/1‑150 (MQ=255)
                                                               aCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAagaaga                                                                                   <  1:4471739/150‑1 (MQ=255)
                                                                ccACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAgaagaa                                                                                  <  2:18981/150‑1 (MQ=255)
                                                                 cACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAAc                                                                                 >  2:1931302/1‑150 (MQ=255)
                                                                      cGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATaaa                                                                            <  1:1057278/150‑1 (MQ=255)
                                                                       gAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCGGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAAc                                                                           <  2:3606203/150‑1 (MQ=255)
                                                                       gAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAAc                                                                           <  2:4235197/150‑1 (MQ=255)
                                                                        aGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATAGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACa                                                                          <  1:2491285/150‑1 (MQ=255)
                                                                         gCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAg                                                                         <  2:4462966/150‑1 (MQ=255)
                                                                           agagAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAgcg                                                                       >  1:861169/1‑150 (MQ=255)
                                                                           agagAAGACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATGTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAATAAGAAGAACATAAACAgcg                                                                       <  2:3183737/150‑1 (MQ=255)
                                                                             agaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGcc                                                                     <  1:3056092/150‑1 (MQ=255)
                                                                              gaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGccc                                                                    >  2:3027520/1‑150 (MQ=255)
                                                                               aaTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCa                                                                   <  2:2238663/150‑1 (MQ=255)
                                                                                  aCCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGt                                                                <  2:5287318/150‑1 (MQ=255)
                                                                                    cGCTCATCGAACCGATCGACAGACCAACGTGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCTGGCGTACGGGTTGCCCAGGACGCCATTAACTGGCCGGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTc                                                              <  1:4615322/150‑1 (MQ=255)
                                                                                    cGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTc                                                              >  2:1829167/1‑150 (MQ=255)
                                                                                    cGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTc                                                              <  2:916967/150‑1 (MQ=255)
                                                                                    cGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTc                                                              <  1:5094993/150‑1 (MQ=255)
                                                                                     gCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCg                                                             <  1:3804600/150‑1 (MQ=255)
                                                                                             aaCCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCa                                                     >  1:4176563/1‑150 (MQ=255)
                                                                                               ccGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAAt                                                   <  1:3749539/150‑1 (MQ=255)
                                                                                               ccGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAAt                                                   <  2:1448931/150‑1 (MQ=255)
                                                                                                cGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATg                                                  <  2:4673838/150‑1 (MQ=255)
                                                                                                  aTCGACAGACCAAACAGAACACCGCCCATTTCAGCTATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCGGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCa                                                <  1:1358139/150‑1 (MQ=255)
                                                                                                    cGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCAtt                                              >  1:1439527/1‑150 (MQ=255)
                                                                                                      aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTAc                                            >  1:3949797/1‑150 (MQ=255)
                                                                                                       cAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCACAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACg                                           >  2:737783/1‑150 (MQ=255)
                                                                                                       cAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACg                                           <  2:1158694/150‑1 (MQ=255)
                                                                                                       cAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACg                                           >  2:127135/1‑150 (MQ=255)
                                                                                                        aGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGt                                          >  2:461322/1‑150 (MQ=255)
                                                                                                        aGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGt                                          >  2:1314437/1‑150 (MQ=255)
                                                                                                         gACCATAGAGAACACCGCCCTTTTCAGCGATCGAGACAGAGATTATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTg                                         <  2:831591/150‑1 (MQ=255)
                                                                                                         gACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTg                                         <  2:5176813/150‑1 (MQ=255)
                                                                                                          aCCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTga                                        >  2:2948421/1‑150 (MQ=255)
                                                                                                           ccAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaa                                       <  1:4856808/150‑1 (MQ=255)
                                                                                                            cAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATTATTTCGCGGATAGCAGGCGTACGGGTTGACAAGGACGCCATTAACAGGCCTGGCAAAATGAATAACATAAACAGCGCCCAGGTTCGTCGTTTCAATGCATTACGTgaag                                      <  1:3440087/150‑1 (MQ=255)
                                                                                                            cAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaag                                      >  1:2543003/1‑150 (MQ=255)
                                                                                                             aaaGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaaga                                     <  2:2439866/150‑1 (MQ=255)
                                                                                                               agagAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAt                                   >  1:3617708/1‑150 (MQ=255)
                                                                                                                 agaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAttt                                 >  2:3457639/1‑150 (MQ=255)
                                                                                                                   aaCACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATACACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTAc                               <  1:2305019/150‑1 (MQ=255)
                                                                                                                   aaCACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTAc                               >  2:336054/1‑150 (MQ=255)
                                                                                                                    acacCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACg                              >  1:3842226/1‑150 (MQ=255)
                                                                                                                     cacCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACgg                             >  1:2062802/1‑150 (MQ=255)
                                                                                                                      acCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGt                            <  2:2135776/150‑1 (MQ=255)
                                                                                                                       ccGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTc                           >  1:1006043/1‑150 (MQ=255)
                                                                                                                        cGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCa                          >  1:645654/1‑150 (MQ=255)
                                                                                                                         gCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCAt                         >  1:2745639/1‑150 (MQ=255)
                                                                                                                            cATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGa                      <  2:1671968/150‑1 (MQ=255)
                                                                                                                             aTTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGAt                     >  1:111271/1‑150 (MQ=255)
                                                                                                                             aTTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGAt                     >  1:1290995/1‑150 (MQ=255)
                                                                                                                             aTTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGAt                     <  2:4760409/150‑1 (MQ=255)
                                                                                                                              tttCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATc                    <  1:838868/150‑1 (MQ=255)
                                                                                                                                 cAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACg                 >  1:1793472/1‑150 (MQ=255)
                                                                                                                                 cAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACg                 >  1:5227836/1‑150 (MQ=255)
                                                                                                                                 cAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACg                 >  1:2799739/1‑150 (MQ=255)
                                                                                                                                  aGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGt                >  2:4447916/1‑150 (MQ=255)
                                                                                                                                     gATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCaa             >  2:2556652/1‑150 (MQ=255)
                                                                                                                                        cGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAAt          <  2:4689880/150‑1 (MQ=255)
                                                                                                                                        cGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAAt          <  1:3720703/150‑1 (MQ=255)
                                                                                                                                        cGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAAt          <  2:3228899/150‑1 (MQ=255)
                                                                                                                                        cGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAAt          <  2:3823230/150‑1 (MQ=255)
                                                                                                                                              agagatAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACTCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTATCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaag    >  2:1236386/1‑150 (MQ=255)
                                                                                                                                              agagatAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaag    >  1:4284987/1‑150 (MQ=255)
                                                                                                                                              agagatAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaag    >  1:1088557/1‑150 (MQ=255)
                                                                                                                                                agataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAACCAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAagag  <  2:2016083/150‑1 (MQ=255)
                                                                                                                                                agataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAagag  >  2:494525/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAG  >  CP009273/882525‑882818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑