BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	942,724	A→T	29.0%	N14Y (AAC→TAC)	ycaM →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	942,724	0	A	T	29.0%	519.1 / 262.7	323	N14Y (AAC→TAC)	ycaM	putative transporter
Reads supporting (aligned to +/- strand): ref base A (109/120); new base T (42/52); total (151/172)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

AATGTGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGG  >  CP009273/942575‑942872

aatGTGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGTAAAACAGTTGCGATGGTaca                                                                                                                                                      <  1:3124156/150‑1 (MQ=255)

aatGTGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTaca                                                                                                                                                      >  2:3336903/1‑150 (MQ=255)

   gtgCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTacaaca                                                                                                                                                   <  2:2717048/150‑1 (MQ=255)

       agcagGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTgc                                                                                                                                               >  1:743605/1‑150 (MQ=255)

          agGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCt                                                                                                                                            <  1:351134/150‑1 (MQ=255)

           ggTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACACCATTGCGCTg                                                                                                                                           >  1:3708480/1‑150 (MQ=255)

             ttATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATAGCGCTGAt                                                                                                                                         >  2:3876438/1‑150 (MQ=255)

             ttATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGAt                                                                                                                                         <  2:311124/150‑1 (MQ=255)

             ttATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGAt                                                                                                                                         >  1:1093371/1‑150 (MQ=255)

              tataATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATg                                                                                                                                        <  1:660934/150‑1 (MQ=255)

               ataATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACAGTGCGCTGATGt                                                                                                                                       >  2:1695990/1‑150 (MQ=255)

                taATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTc                                                                                                                                      >  2:3466258/1‑150 (MQ=255)

                  aTTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCtt                                                                                                                                    >  2:1291118/1‑150 (MQ=255)

                  aTTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCtt                                                                                                                                    >  2:2926407/1‑150 (MQ=255)

                     ttGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTa                                                                                                                                 <  2:4771496/150‑1 (MQ=255)

                     ttGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTa                                                                                                                                 <  1:2211218/150‑1 (MQ=255)

                      tGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTAt                                                                                                                                >  1:5269978/1‑150 (MQ=255)

                      tGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTAt                                                                                                                                <  1:284199/150‑1 (MQ=255)

                       gCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATc                                                                                                                               <  2:1842193/150‑1 (MQ=255)

                        cATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCa                                                                                                                              >  1:4352078/1‑150 (MQ=255)

                        cATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCa                                                                                                                              >  2:4250437/1‑150 (MQ=255)

                           ttCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTg                                                                                                                           <  1:2476237/150‑1 (MQ=255)

                           ttCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTAACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTg                                                                                                                           >  1:979348/1‑150 (MQ=255)

                             cGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTc                                                                                                                         >  2:3427987/1‑150 (MQ=255)

                              gCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCt                                                                                                                        >  1:2257099/1‑150 (MQ=255)

                               cTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTg                                                                                                                       >  2:451313/1‑150 (MQ=255)

                               cTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTg                                                                                                                       >  1:166059/1‑150 (MQ=255)

                                tATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTgg                                                                                                                      >  2:843609/1‑150 (MQ=255)

                                 aTTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTggg                                                                                                                     <  1:1609645/150‑1 (MQ=255)

                                   ttCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTggggc                                                                                                                   <  2:2363430/150‑2 (MQ=255)

                                   ttCCGCACGTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGt                                                                                                                   >  2:4018168/1‑150 (MQ=255)

                                       gCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTg                                                                                                               <  1:4061996/150‑1 (MQ=255)

                                        cACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACACCATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTgg                                                                                                              >  2:4262664/1‑150 (MQ=255)

                                         aCTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGc                                                                                                             >  2:123035/1‑150 (MQ=255)

                                         aCTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGc                                                                                                             >  1:1826356/1‑150 (MQ=255)

                                               tATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACgtt                                                                                                       <  2:1601422/150‑1 (MQ=255)

                                                  ttGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTCCATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACgttgtt                                                                                                    <  2:974387/150‑1 (MQ=255)

                                                   tGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTta                                                                                                   <  1:4183809/150‑1 (MQ=255)

                                                     ccGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTtaat                                                                                                 <  1:1359847/150‑1 (MQ=255)

                                                        cgcATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGAGTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAAc                                                                                              >  1:1743307/1‑150 (MQ=255)

                                                          cATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTa                                                                                            >  2:2047382/1‑150 (MQ=255)

                                                           aTAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTAt                                                                                           >  2:4441155/1‑150 (MQ=255)

                                                             aaTCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGc                                                                                         <  1:5208448/150‑1 (MQ=255)

                                                              aTCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGcc                                                                                        <  2:4676798/150‑1 (MQ=255)

                                                              aTCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGcc                                                                                        <  1:1011750/150‑1 (MQ=255)

                                                                 catCGTTTTACCGATGCCCCTTTAATTTTGGCGAATGATTTGTCTATGGCAGTGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTGTTTTATCACTGTCGGGGGTTTTGGCAACGTAGTTAATAACTAAGCCAAc                                                                                     >  2:1771334/3‑150 (MQ=255)

                                                                  cTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAAcc                                                                                    >  1:4828874/1‑150 (MQ=255)

                                                                     gTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAgg                                                                                 <  1:3557928/150‑1 (MQ=255)

                                                                        ttACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGc                                                                              <  2:5245283/150‑1 (MQ=255)

                                                                        ttACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCGGGGACTGTTCAGGAAAAACAGTTGCGATGGTCCAACATTGCGCTGATGTCTTTTATCACTGTCGGGGGTTTTGGCAACGGTGTTAATAACTATGCCAACCAGGGGc                                                                              >  2:4921138/1‑150 (MQ=255)

                                                                            cGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCtggt                                                                          <  1:3890605/150‑1 (MQ=255)

                                                                            cGATGCACCTTTAATTTTGGCGAATGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGAGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCtggt                                                                          <  1:4884271/150‑1 (MQ=255)

                                                                               tGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTggtggt                                                                       <  1:315813/150‑1 (MQ=255)

                                                                                 ccccTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGgttg                                                                     <  2:17236/150‑1 (MQ=255)

                                                                                  cccTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGgttgt                                                                    <  1:3322334/150‑1 (MQ=255)

                                                                                  cccTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGgttgt                                                                    <  1:193289/150‑1 (MQ=255)

                                                                                   ccTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGgttgtt                                                                   <  1:817500/150‑1 (MQ=255)

                                                                                    cTTTAATTTTGGCGAAGGATTATTATATGGCTGTGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGACAACAATTGGCTGGTgtttgttt                                                                  >  2:2369971/1‑150 (MQ=255)

                                                                                        aaTTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTc                                                              <  1:2078822/150‑1 (MQ=255)

                                                                                         aTTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCa                                                             >  2:3563330/1‑150 (MQ=255)

                                                                                           tttGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATg                                                           <  2:4382297/150‑1 (MQ=255)

                                                                                           tttGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATg                                                           <  1:1454055/150‑1 (MQ=255)

                                                                                            ttGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATgg                                                          >  2:3229797/1‑150 (MQ=255)

                                                                                                cGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGgtgt                                                      >  1:1287151/1‑150 (MQ=255)

                                                                                                cGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGgtgt                                                      >  1:4236230/1‑150 (MQ=255)

                                                                                                  aaGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGttt                                                    >  2:3672115/1‑150 (MQ=255)

                                                                                                    ggATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTAt                                                  >  1:4927340/1‑150 (MQ=255)

                                                                                                    ggATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGtttct                                                  >  2:694369/1‑148 (MQ=255)

                                                                                                    ggATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTAt                                                  <  2:4944654/150‑1 (MQ=255)

                                                                                                      aTTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCt                                                <  2:2877090/150‑1 (MQ=255)

                                                                                                      aTTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTGATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCt                                                <  2:190230/150‑1 (MQ=255)

                                                                                                       tttGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCtt                                               >  1:1230628/1‑150 (MQ=255)

                                                                                                        ttGTCTATGGCTGGGAATGTTCAGGAAAAACGGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCttt                                              <  2:2588291/150‑1 (MQ=255)

                                                                                                        ttGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCttt                                              <  1:2109149/150‑1 (MQ=255)

                                                                                                         tGTCTATGGCTTTGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTTCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTg                                             <  1:2653421/150‑1 (MQ=255)

                                                                                                            cTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCAc                                          >  1:2810256/1‑150 (MQ=255)

                                                                                                              aTGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCActc                                        <  2:538996/150‑1 (MQ=255)

                                                                                                                  cTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTAtt                                    <  1:2333689/150‑1 (MQ=255)

                                                                                                                  cTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTAtt                                    <  2:1444059/150‑1 (MQ=255)

                                                                                                                  cTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTAtt                                    >  2:3917458/1‑150 (MQ=255)

                                                                                                                    gggAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTc                                  <  2:2624254/150‑1 (MQ=255)

                                                                                                                      gAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTcac                                >  1:4632333/1‑150 (MQ=255)

                                                                                                                       aaTGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTcaca                               >  1:471855/1‑150 (MQ=255)

                                                                                                                          tttCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCt                            <  2:2769815/149‑1 (MQ=255)

                                                                                                                          gTTCAGGAAAAACAGTTGAGATGGTACAACATTGCGCTGATGTCTATTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCt                            <  2:111303/150‑1 (MQ=255)

                                                                                                                           ttCAGGAAAAACAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCtt                           >  2:5012231/1‑150 (MQ=255)

                                                                                                                             cAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTAt                         <  2:4336205/150‑1 (MQ=255)

                                                                                                                             cAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTAt                         <  1:283261/150‑1 (MQ=255)

                                                                                                                                      cAGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTGATGCGCTAAtt                >  2:794808/1‑150 (MQ=255)

                                                                                                                                       aGTTGCGATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAAttg               >  1:2606253/1‑150 (MQ=255)

                                                                                                                                       aGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAAttg               <  2:2294878/150‑1 (MQ=255)

                                                                                                                                        gTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAAttgt              >  2:3063161/1‑150 (MQ=255)

                                                                                                                                          tGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCACCCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCTCACCTTATGCGCTAAttgttg            <  2:908173/150‑1 (MQ=255)

                                                                                                                                             gATGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTc         <  2:2305415/150‑1 (MQ=255)

                                                                                                                                             gATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTc         >  1:5072832/1‑150 (MQ=255)

                                                                                                                                              aTGGTACTACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCa        <  1:4003447/150‑1 (MQ=255)

                                                                                                                                                  tACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTa    <  1:578595/150‑1 (MQ=255)

                                                                                                                                                    caacaTTGCGCTGATGTCTTTTATCTCTGTCTGGGGTTTTGTCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAgg  <  1:4480085/150‑1 (MQ=255)

                                                                                                                                                    caacaTTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAgg  >  1:1558037/1‑150 (MQ=255)

                                                                                                                                                    caacaTTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAgg  >  2:658189/1‑150 (MQ=255)

AATGTGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTATCTTTGCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGG  >  CP009273/942575‑942872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`