Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,113,450 A→T 100% I161I (ATT→ATA)  yceI ← secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,113,4500AT99.1% 1024.9 / ‑1.5 314I161I (ATT→ATA) yceIsecreted protein
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base T (182/129);  total (185/129)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 315 total aligned reads displayed.

TCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCAC  >  CP009273/1113303‑1113599
                                                                                                                                                   |                                                                                                                                                     
tCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAt                                                                                                                                                     <  1:771003/150‑1 (MQ=255)
tCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAt                                                                                                                                                     >  1:3606461/1‑150 (MQ=255)
   aaCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAtttt                                                                                                                                                  >  1:973968/1‑150 (MQ=255)
     cAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGc                                                                                                                                                >  1:3669416/1‑150 (MQ=255)
       gAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCt                                                                                                                                              >  2:3649884/1‑150 (MQ=255)
        aataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCtt                                                                                                                                             >  2:2913849/1‑150 (MQ=255)
        aataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCtt                                                                                                                                             >  2:322384/1‑150 (MQ=255)
        aataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCtt                                                                                                                                             >  2:3296705/1‑150 (MQ=255)
              tatCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCt                                                                                                                                       <  1:3901552/150‑1 (MQ=255)
              tatCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCt                                                                                                                                       <  2:4611409/150‑1 (MQ=255)
                tCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCg                                                                                                                                     <  2:1692600/150‑1 (MQ=255)
                tCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCg                                                                                                                                     >  1:3844542/1‑150 (MQ=255)
                 cATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGa                                                                                                                                    >  1:1046258/1‑150 (MQ=255)
                  atagCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGaa                                                                                                                                   >  1:3116280/4‑150 (MQ=255)
                  aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATAGTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGaa                                                                                                                                   >  2:3032221/1‑150 (MQ=255)
                     gCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGTTATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGcc                                                                                                                                >  2:4592965/1‑150 (MQ=255)
                        ggATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGc                                                                                                                             >  2:3958818/1‑150 (MQ=255)
                              gTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGttt                                                                                                                       >  2:4204058/1‑150 (MQ=255)
                              gTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGttt                                                                                                                       <  1:1027126/150‑1 (MQ=255)
                                 ccATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTacc                                                                                                                    >  2:5225503/1‑150 (MQ=255)
                                  cATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTacca                                                                                                                   <  2:516100/150‑1 (MQ=255)
                                  cATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTacca                                                                                                                   >  2:2609250/1‑150 (MQ=255)
                                  cATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAAGCTGGCTTGATATTGAAGTCCTTGAGCGTTATTTTGCCTGCGGCCTCGAAGCCTGCACGTTTacca                                                                                                                   >  2:2082491/1‑150 (MQ=255)
                                   aTCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTaccac                                                                                                                  >  2:3490749/1‑150 (MQ=255)
                                     ccGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCAccc                                                                                                                >  1:4540070/1‑150 (MQ=255)
                                      cGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCAcccc                                                                                                               >  2:1030103/1‑150 (MQ=255)
                                      cGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCAcccc                                                                                                               <  1:3384601/150‑1 (MQ=255)
                                      cGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCAcccc                                                                                                               <  2:1092522/150‑1 (MQ=255)
                                         cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATg                                                                                                            >  2:2857320/1‑150 (MQ=255)
                                         cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATg                                                                                                            >  1:4371575/1‑150 (MQ=255)
                                            cATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCGGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCGTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGgt                                                                                                         >  2:4251917/1‑150 (MQ=255)
                                              tttCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGgtcg                                                                                                       <  2:5320319/150‑1 (MQ=255)
                                                tCACGCTTACTACTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGAATACCACCCCAAGGgtcgtc                                                                                                     >  2:1363161/1‑150 (MQ=255)
                                                          ttCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCa                                                                                           <  2:815959/150‑1 (MQ=255)
                                                           tCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCaa                                                                                          >  1:2225325/1‑150 (MQ=255)
                                                                  tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAAttt                                                                                   >  2:414118/1‑150 (MQ=255)
                                                                   aCGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTc                                                                                  >  1:5200468/1‑150 (MQ=255)
                                                                   aCGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTc                                                                                  <  1:208726/150‑1 (MQ=255)
                                                                   aCGCCTCCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTc                                                                                  <  2:1917564/150‑1 (MQ=255)
                                                                    cgacTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCg                                                                                 >  1:3219193/4‑150 (MQ=255)
                                                                       cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCtt                                                                              >  2:888217/1‑150 (MQ=255)
                                                                        ttCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTc                                                                             >  1:4957891/1‑150 (MQ=255)
                                                                          ccACTGAAATANTCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCa                                                                           >  1:2761996/1‑150 (MQ=255)
                                                                           cACTGAAATAATCAGATCCACTTCCTGAGAAGNTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCan                                                                          <  1:635172/150‑2 (MQ=255)
                                                                           cACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAg                                                                          >  2:745332/1‑150 (MQ=255)
                                                                            aCTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGc                                                                         >  1:3766570/1‑150 (MQ=255)
                                                                               gAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCCTGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTg                                                                      <  2:5069220/150‑1 (MQ=255)
                                                                               gAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTg                                                                      <  2:4699731/150‑1 (MQ=255)
                                                                                aaaTAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa                                                                     >  2:2062489/1‑150 (MQ=255)
                                                                                aaaTAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa                                                                     >  2:151780/1‑150 (MQ=255)
                                                                                   taatCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACgg                                                                  >  2:2414572/1‑150 (MQ=255)
                                                                                    aatCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACggg                                                                 >  2:4940440/1‑150 (MQ=255)
                                                                                       cAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTATGCCTTCGTCCACGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGATTCCAGCGTGACGGGgtt                                                              >  2:1214794/1‑150 (MQ=255)
                                                                                        aGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTg                                                             >  1:3496944/1‑150 (MQ=255)
                                                                                        aGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTg                                                             <  1:1794834/150‑1 (MQ=255)
                                                                                        aGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTg                                                             >  2:1654256/1‑150 (MQ=255)
                                                                                             cACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTAc                                                        >  2:4048083/1‑150 (MQ=255)
                                                                                                    tGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTc                                                 >  1:4336101/1‑150 (MQ=255)
                                                                                                    tGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTc                                                 <  1:147655/150‑1 (MQ=255)
                                                                                                    tGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTc                                                 >  1:474445/1‑150 (MQ=255)
                                                                                                     gagaAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCa                                                <  2:2235619/150‑1 (MQ=255)
                                                                                                     gagaAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCa                                                >  1:5290325/1‑150 (MQ=255)
                                                                                                     gagaAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCa                                                >  1:2649863/1‑150 (MQ=255)
                                                                                                      agaAGCTGGACCTAACTCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAg                                               <  2:2684972/150‑1 (MQ=255)
                                                                                                       gaAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGc                                              >  1:4426288/1‑150 (MQ=255)
                                                                                                            tGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCCTTCAGCGTCAg                                         >  2:1420645/1‑150 (MQ=255)
                                                                                                             ggACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGa                                        >  1:1269926/1‑150 (MQ=255)
                                                                                                             ggACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGa                                        >  1:3343628/1‑150 (MQ=255)
                                                                                                              gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTTATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                       <  2:3785832/150‑1 (MQ=255)
                                                                                                              gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                       <  2:4837229/150‑1 (MQ=255)
                                                                                                              gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                       >  1:2414660/1‑150 (MQ=255)
                                                                                                              gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                       >  2:3815351/1‑150 (MQ=255)
                                                                                                              gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                       <  2:4025066/150‑1 (MQ=255)
                                                                                                              gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                       <  2:273180/150‑1 (MQ=255)
                                                                                                               aCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATc                                      <  1:5225503/150‑1 (MQ=255)
                                                                                                                ccTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCg                                     <  1:1146316/150‑1 (MQ=255)
                                                                                                                  tAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCCTGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGcc                                   <  1:1382719/150‑1 (MQ=255)
                                                                                                                  tAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGcc                                   <  1:3062436/150‑1 (MQ=255)
                                                                                                                  tAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGcc                                   <  1:3854340/150‑1 (MQ=255)
                                                                                                                     aTCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGt                                >  1:1178733/1‑150 (MQ=255)
                                                                                                                     aTCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGt                                >  1:3293733/1‑150 (MQ=255)
                                                                                                                     aTCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATACAGCGTCAGATCGCCGGt                                >  2:2542116/1‑150 (MQ=255)
                                                                                                                      tCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTa                               >  2:1281642/1‑150 (MQ=255)
                                                                                                                        tGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAat                             >  2:4413672/1‑150 (MQ=255)
                                                                                                                        tGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAat                             <  1:3932996/150‑1 (MQ=255)
                                                                                                                         gTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCTCCGGTAata                            >  1:449175/1‑150 (MQ=255)
                                                                                                                         gTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAata                            >  1:4272485/1‑150 (MQ=255)
                                                                                                                            ttGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATcc                         >  1:1335444/1‑150 (MQ=255)
                                                                                                                             tGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCa                        >  1:3035364/1‑150 (MQ=255)
                                                                                                                             tGATATTGAAGTCCTTGAGCTTTATATTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCa                        <  2:356182/150‑1 (MQ=255)
                                                                                                                              gATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAg                       <  1:4690430/150‑1 (MQ=255)
                                                                                                                              gATATTGAAGTCCTTGAGCTTTATTTTGCCGTCGGCCTCGAAGCCTGCACGTTTACCACCCCCTGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAg                       <  2:1774388/150‑1 (MQ=255)
                                                                                                                               atatTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGt                      <  1:1505597/150‑1 (MQ=255)
                                                                                                                                    gAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTc                 >  2:1200318/1‑150 (MQ=255)
                                                                                                                                         ccTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGt            >  1:2962268/1‑150 (MQ=255)
                                                                                                                                             gAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtcttt        <  1:5055165/150‑1 (MQ=255)
                                                                                                                                              aGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtctttc       >  2:4477363/1‑150 (MQ=255)
                                                                                                                                              aGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtctttc       <  1:4788836/150‑1 (MQ=255)
                                                                                                                                                cTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtctttctt     <  2:2900228/150‑1 (MQ=255)
                                                                                                                                                   aaTTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCCTTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCAc  >  2:660914/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCAC  >  CP009273/1113303‑1113599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑