Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,122,429 G→T 12.6% S56S (TCG→TCT)  yceM → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,122,4290GT12.6% 779.7 / 56.7 241S56S (TCG→TCT) yceMputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (109/101);  new base T (15/16);  total (124/117)
Fisher's exact test for biased strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCT  >  CP009273/1122280‑1122578
                                                                                                                                                     |                                                                                                                                                     
  cGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGGGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGtt                                                                                                                                                     <  2:3910376/150‑1 (MQ=255)
  cGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGtt                                                                                                                                                     <  2:2615836/150‑1 (MQ=255)
   gTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTa                                                                                                                                                    <  1:2904365/150‑1 (MQ=255)
   gTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTa                                                                                                                                                    >  1:2090480/1‑150 (MQ=255)
    tAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTAt                                                                                                                                                   <  2:4041188/150‑1 (MQ=255)
     aGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATc                                                                                                                                                  >  1:2438440/1‑150 (MQ=255)
     aGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGACGATTCGTTATc                                                                                                                                                  >  2:3683968/1‑150 (MQ=255)
      gTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATcc                                                                                                                                                 <  2:4761365/150‑1 (MQ=255)
              aGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTgc                                                                                                                                         <  2:1639651/150‑1 (MQ=255)
                    cATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAg                                                                                                                                   <  2:1226260/150‑1 (MQ=255)
                     aTTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGt                                                                                                                                  >  1:1079903/1‑150 (MQ=255)
                      ttGCGCAAAAAGTGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGtt                                                                                                                                 <  1:4369691/150‑1 (MQ=255)
                      ttGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGtt                                                                                                                                 <  1:1960892/150‑1 (MQ=255)
                      ttGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGtt                                                                                                                                 <  2:5117914/150‑1 (MQ=255)
                       tGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTg                                                                                                                                <  1:3383353/150‑1 (MQ=255)
                            aaaaaGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATg                                                                                                                           >  2:2826757/1‑150 (MQ=255)
                              aaaGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCg                                                                                                                         <  1:3606201/150‑1 (MQ=255)
                               aaGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCgg                                                                                                                        >  2:4554756/1‑150 (MQ=255)
                                 gCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGtt                                                                                                                      <  1:2821864/150‑1 (MQ=255)
                                 gCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGtt                                                                                                                      >  1:4533529/1‑150 (MQ=255)
                                 gCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGtt                                                                                                                      >  1:491040/1‑150 (MQ=255)
                                      gTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTtgt                                                                                                                 <  1:3614930/150‑1 (MQ=255)
                                       ttACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTtgtg                                                                                                                >  2:2331047/1‑150 (MQ=255)
                                         aCCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCa                                                                                                              <  1:4560556/150‑1 (MQ=255)
                                           cGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCAtt                                                                                                            <  2:4545953/150‑1 (MQ=255)
                                            ggTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTc                                                                                                           >  2:475201/1‑150 (MQ=255)
                                            ggTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTc                                                                                                           >  2:3345430/1‑150 (MQ=255)
                                                  ggcggcAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCAc                                                                                                     >  1:3828734/1‑150 (MQ=255)
                                                    cggcAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCg                                                                                                   >  2:3837416/1‑150 (MQ=255)
                                                     ggcAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgc                                                                                                  >  1:5064843/1‑150 (MQ=255)
                                                       cAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGACAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCCTTCCAGCACCgcca                                                                                                >  2:2778822/1‑150 (MQ=255)
                                                       cAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgcca                                                                                                <  2:108750/150‑1 (MQ=255)
                                                       cAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgcca                                                                                                >  1:466288/1‑150 (MQ=255)
                                                        aGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTCTCCAGCCTTGCCGCCAGTTGCGATGCGGTTGTTGTGCATTCCAGCACCgccag                                                                                               >  2:591228/1‑150 (MQ=255)
                                                           gTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTACCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgccagcca                                                                                            >  2:2286146/1‑150 (MQ=255)
                                                               gACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACttt                                                                                        >  2:5015533/1‑150 (MQ=255)
                                                               gACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACttt                                                                                        <  2:3589185/150‑1 (MQ=255)
                                                                  tGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGAc                                                                                     <  1:95853/150‑1 (MQ=255)
                                                                  tGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGAc                                                                                     >  1:458558/1‑150 (MQ=255)
                                                                       gTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGt                                                                                <  2:2484261/150‑1 (MQ=255)
                                                                        ttACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTc                                                                               <  1:2541419/150‑1 (MQ=255)
                                                                        ttACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTc                                                                               <  1:3345178/150‑1 (MQ=255)
                                                                         tACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCa                                                                              <  1:1273513/150‑1 (MQ=255)
                                                                           cAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGt                                                                            <  2:2539195/150‑1 (MQ=255)
                                                                           cAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGt                                                                            >  1:649495/1‑150 (MQ=255)
                                                                           cAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGt                                                                            <  2:4153319/150‑1 (MQ=255)
                                                                           cAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGt                                                                            <  2:5183513/150‑1 (MQ=255)
                                                                            aaGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTa                                                                           <  2:3986261/150‑1 (MQ=255)
                                                                             aGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTAc                                                                          >  1:1700976/1‑150 (MQ=255)
                                                                             aGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTAc                                                                          >  2:1452252/1‑150 (MQ=255)
                                                                              ggAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACg                                                                         <  1:4804287/150‑1 (MQ=255)
                                                                              ggAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACg                                                                         <  2:1107170/150‑1 (MQ=255)
                                                                                aGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGtt                                                                       >  2:2064007/1‑150 (MQ=255)
                                                                                 gCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTa                                                                      <  1:4220429/150‑1 (MQ=255)
                                                                                  ccTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTAc                                                                     <  1:827426/150‑1 (MQ=255)
                                                                                   cTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACt                                                                    <  1:2166028/150‑1 (MQ=255)
                                                                                    tGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTc                                                                   <  1:1229196/150‑1 (MQ=255)
                                                                                     ggTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCa                                                                  <  1:4374825/150‑1 (MQ=255)
                                                                                      gTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCaa                                                                 <  2:1243686/150‑1 (MQ=255)
                                                                                        cGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATg                                                               <  2:4307861/150‑1 (MQ=255)
                                                                                        cGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATg                                                               <  1:2305510/150‑1 (MQ=255)
                                                                                        cGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATg                                                               <  1:4490451/150‑1 (MQ=255)
                                                                                         gCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGAACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGc                                                              >  1:3533335/1‑150 (MQ=255)
                                                                                               gcgcgcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGt                                                        >  2:94151/1‑150 (MQ=255)
                                                                                                    cgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATg                                                   >  2:1937663/1‑150 (MQ=255)
                                                                                                     gAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGt                                                  >  2:3203979/1‑150 (MQ=255)
                                                                                                      aaaGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTc                                                 >  1:3214445/1‑150 (MQ=255)
                                                                                                      aaaGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTc                                                 >  1:1668227/1‑150 (MQ=255)
                                                                                                       aaGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCt                                                <  1:3775077/150‑1 (MQ=255)
                                                                                                       aaGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCt                                                <  1:2419194/150‑1 (MQ=255)
                                                                                                        aGCCCTGCCAATTTGTGAAAGCTGTCGCATTCCTTATGCCGATTAGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGACACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCtg                                               <  1:3307312/150‑1 (MQ=255)
                                                                                                         gCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCtgt                                              >  2:3129269/1‑150 (MQ=255)
                                                                                                            cTGCCAATTTGTGCAAGCTGGCGCATTCCTTCTGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTc                                           <  2:1630292/150‑1 (MQ=255)
                                                                                                             tGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGGACGTTACTCAATGCGGGGGTACATGTCTGTGTCg                                          >  2:141299/1‑150 (MQ=255)
                                                                                                               ccAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGAt                                        <  1:5275673/150‑1 (MQ=255)
                                                                                                                cAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATa                                       >  1:4049133/1‑150 (MQ=255)
                                                                                                                cAATTTGTGAAAGCTGGCGCATTCCTTATGCCGAGTCGTTATCCAGCCTTGCCGCCAGGTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCACTGCGGGGGTACATGTCTGTGTCGATa                                       <  2:2951033/150‑1 (MQ=255)
                                                                                                                 aaTTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATaa                                      >  1:1870652/1‑150 (MQ=255)
                                                                                                                    ttGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAAcc                                   <  2:2748798/150‑1 (MQ=255)
                                                                                                                      gtgAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGc                                 >  1:2054724/1‑150 (MQ=255)
                                                                                                                       tgAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCt                                <  2:3359433/150‑1 (MQ=255)
                                                                                                                                 cgcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAAt                      <  1:441548/150‑1 (MQ=255)
                                                                                                                                  gcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATc                     >  2:4931212/1‑150 (MQ=255)
                                                                                                                                    aTTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTg                   >  1:3407372/1‑150 (MQ=255)
                                                                                                                                    aTTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTg                   >  1:1525548/1‑150 (MQ=255)
                                                                                                                                    aTTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTg                   >  1:61694/1‑150 (MQ=255)
                                                                                                                                     ttCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgc                  >  2:2381350/1‑150 (MQ=255)
                                                                                                                                     ttCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCAGTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgc                  <  2:3456549/150‑1 (MQ=255)
                                                                                                                                      tCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgcg                 <  1:299240/150‑1 (MQ=255)
                                                                                                                                       ccTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgcgc                >  2:4870762/1‑150 (MQ=255)
                                                                                                                                          tATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGAt             >  1:2970520/1‑150 (MQ=255)
                                                                                                                                             gCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCt          <  2:5064843/150‑1 (MQ=255)
                                                                                                                                              ccGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTg         <  2:555555/150‑1 (MQ=255)
                                                                                                                                               cGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGTGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGa        >  1:514867/1‑150 (MQ=255)
                                                                                                                                               cGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGa        >  1:1752497/1‑150 (MQ=255)
                                                                                                                                               cGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGa        >  2:3239302/1‑150 (MQ=255)
                                                                                                                                                 aTTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAAc      >  1:2027622/1‑150 (MQ=255)
                                                                                                                                                  ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACg     <  2:738177/150‑1 (MQ=255)
                                                                                                                                                  ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACg     <  1:1205965/150‑1 (MQ=255)
                                                                                                                                                     gTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCt  >  2:3525772/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCT  >  CP009273/1122280‑1122578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑