BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,128,764	C→A	28.8%	I245I (ATC→ATA)	flgE →	flagellar hook protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,128,764	0	C	A	28.8%	552.8 / 190.3	280	I245I (ATC→ATA)	flgE	flagellar hook protein
Reads supporting (aligned to +/- strand): ref base C (101/98); new base A (40/41); total (141/139)
Fisher's exact test for biased strand distribution p-value = 8.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 286 total aligned reads displayed.

ATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGA  >  CP009273/1128615‑1128913

atgaGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATc                                                                                                                                                       >  2:1556586/1‑150 (MQ=255)

 tgaGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCg                                                                                                                                                      >  2:4888097/1‑150 (MQ=255)

  gaGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGc                                                                                                                                                     >  2:3741805/1‑150 (MQ=255)

  gaGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGc                                                                                                                                                     <  2:1710140/150‑1 (MQ=255)

      gTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAAcc                                                                                                                                                 >  1:3162186/1‑150 (MQ=255)

         tACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGgc                                                                                                                                              <  1:4688082/150‑1 (MQ=255)

          aCTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGgcg                                                                                                                                             >  2:3604857/1‑150 (MQ=255)

                tgAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTa                                                                                                                                       <  2:2815574/150‑1 (MQ=255)

                 gAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTaa                                                                                                                                      >  2:3212639/1‑150 (MQ=255)

                      ccGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTg                                                                                                                                 <  2:3622669/150‑1 (MQ=255)

                        ggggATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCa                                                                                                                               <  1:2356597/150‑1 (MQ=255)

                         gggATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAg                                                                                                                              >  2:3022304/1‑150 (MQ=255)

                          ggATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGa                                                                                                                             <  1:4545207/150‑1 (MQ=255)

                           gATTATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTCATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGaa                                                                                                                            >  2:4931266/1‑150 (MQ=255)

                           gATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGaa                                                                                                                            >  2:4810103/1‑150 (MQ=255)

                            ataataACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAAc                                                                                                                           <  1:384738/150‑1 (MQ=255)

                             taataaCTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAAcc                                                                                                                          >  2:2513368/1‑150 (MQ=255)

                               ataaCTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCg                                                                                                                        <  1:5268455/150‑1 (MQ=255)

                                 aaCTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGcc                                                                                                                      >  1:4982381/1‑150 (MQ=255)

                                      gCAGGTCTACACCCAGGATAGCAGTGATCCAAACCGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGtt                                                                                                                 <  2:1074129/150‑1 (MQ=255)

                                       cAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCTACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGttt                                                                                                                >  2:1985928/1‑150 (MQ=255)

                                        aGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTa                                                                                                               >  1:5115772/1‑150 (MQ=255)

                                        aGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTa                                                                                                               >  1:1832123/1‑150 (MQ=255)

                                            cTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCt                                                                                                           >  1:2329226/1‑150 (MQ=255)

                                            cTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCt                                                                                                           <  1:3756421/150‑1 (MQ=255)

                                                acCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGc                                                                                                       >  2:2451876/1‑150 (MQ=255)

                                                acCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGc                                                                                                       >  2:4772011/1‑150 (MQ=255)

                                                   cAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTc                                                                                                    >  1:4891854/1‑150 (MQ=255)

                                                   cAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTc                                                                                                    >  2:4706643/1‑150 (MQ=255)

                                                      gATAGCAGTGATCCAATCAGCTGTGCGAAGTCAGCGACAACACTGGAATTTAATGCTTCTGGCACATTAGTGGATGGTGCGCTGGCGAATACTATCGCAACCGGCGCACTTAACGGTGAAGAACACGCCACGTTTAGTCTGAGCTTCCTc                                                                                                 <  2:239047/150‑1 (MQ=255)

                                                      gATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGCATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGACTAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTATAGTCTGAGCTTCCTc                                                                                                 >  2:4760652/1‑150 (MQ=255)

                                                      gATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTc                                                                                                 <  2:2134603/150‑1 (MQ=255)

                                                      gATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTc                                                                                                 >  2:3861366/1‑150 (MQ=255)

                                                      gATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTc                                                                                                 <  1:4780149/150‑1 (MQ=255)

                                                      gATAGCAGTGATCCAAACAGCAGTGCGAATACAGCGACAACTATGGAATTTACTGCTAATGGCACATTAGTGGATGGTGCGATGTCGAATACTATCGCAACCGGCGCACTTACCGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTc                                                                                                 <  2:2047939/150‑1 (MQ=255)

                                                          gCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACt                                                                                             <  1:2653665/150‑1 (MQ=255)

                                                          gCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACt                                                                                             <  1:3971774/150‑1 (MQ=255)

                                                          gCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACt                                                                                             <  1:5258835/150‑1 (MQ=255)

                                                              tGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCAt                                                                                         <  1:282340/150‑1 (MQ=255)

                                                                     aaCAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCaa                                                                                  >  1:2066170/1‑150 (MQ=255)

                                                                      aCAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCaaa                                                                                 <  1:677391/150‑1 (MQ=255)

                                                                        aGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAAt                                                                               >  1:73923/1‑150 (MQ=255)

                                                                         gCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATa                                                                              <  2:3412641/150‑1 (MQ=255)

                                                                          cATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATAc                                                                             >  1:3332998/1‑150 (MQ=255)

                                                                            ttGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCg                                                                           >  1:3576957/1‑150 (MQ=255)

                                                                              gCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGgc                                                                         >  1:4243159/1‑150 (MQ=255)

                                                                               cGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGgcg                                                                        <  1:521554/150‑1 (MQ=255)

                                                                               cGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGgcg                                                                        <  1:1796632/150‑1 (MQ=255)

                                                                                 aaGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCt                                                                      >  2:2929998/1‑150 (MQ=255)

                                                                                  aGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTa                                                                     >  1:2157516/1‑150 (MQ=255)

                                                                                  aGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTa                                                                     <  1:5079925/150‑1 (MQ=255)

                                                                                    aCAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAAc                                                                   <  1:5275785/150‑1 (MQ=255)

                                                                                      aGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAAACGTCGCAATTAACTGTGCAGAACCCGCCACGTTTATTCTGAGCTTCCTCAACTCCATGAAGCAAAATACCGGCGCTAACaa                                                                 >  1:1296299/1‑150 (MQ=255)

                                                                                       gCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAat                                                                <  2:2715052/150‑1 (MQ=255)

                                                                                         gACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAatat                                                              >  1:1067161/1‑150 (MQ=255)

                                                                                         gACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAatat                                                              >  2:180000/1‑150 (MQ=255)

                                                                                          acaacaCTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATAtt                                                             >  2:3534406/1‑150 (MQ=255)

                                                                                            aacaGTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGAGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGATCTTACTCAACTCCATGCAGCAAAATACCGGCGCTATCAATATtgt                                                           <  2:1037718/150‑1 (MQ=255)

                                                                                            aacaCTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATtgt                                                           >  2:194874/1‑150 (MQ=255)

                                                                                             acacTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATtgtg                                                          <  2:246515/150‑1 (MQ=255)

                                                                                             acacTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATtgtg                                                          >  2:4287057/1‑150 (MQ=255)

                                                                                              cacTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTgg                                                         >  2:542768/1‑150 (MQ=255)

                                                                                               acTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGc                                                        <  2:3966999/150‑1 (MQ=255)

                                                                                                cTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCa                                                       <  1:1526414/150‑1 (MQ=255)

                                                                                                  ggAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAac                                                     >  1:223432/1‑150 (MQ=255)

                                                                                                  ggAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAac                                                     <  1:4404403/150‑1 (MQ=255)

                                                                                                   gAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAacc                                                    <  2:4281490/150‑1 (MQ=255)

                                                                                                        tAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCa                                               >  2:4234728/1‑150 (MQ=255)

                                                                                                          aTGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGa                                             >  1:3632965/1‑150 (MQ=255)

                                                                                                          aTGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGa                                             >  2:2892347/1‑150 (MQ=255)

                                                                                                           tGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGaa                                            <  2:5235403/150‑1 (MQ=255)

                                                                                                             cTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACg                                          <  1:2470708/150‑1 (MQ=255)

                                                                                                             cTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACg                                          >  1:1417367/1‑150 (MQ=255)

                                                                                                             cTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACg                                          <  2:125337/150‑1 (MQ=255)

                                                                                                                aTGGCTCATTAGTGGATGGTGCGATGGCGAATAATATCGCAACAGGCGCAATTAACGGTGCAGAACACGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGAAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCt                                       <  2:2349860/150‑1 (MQ=255)

                                                                                                                     acaTTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACaaa                                  >  2:4423600/1‑150 (MQ=255)

                                                                                                                     acaTTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGGTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACaaa                                  >  2:1897894/1‑150 (MQ=255)

                                                                                                                      caTTAGTGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAAc                                 >  1:1097058/1‑150 (MQ=255)

                                                                                                                       aTTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAAcc                                <  2:711230/150‑1 (MQ=255)

                                                                                                                           gTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGc                            >  1:3409902/1‑150 (MQ=255)

                                                                                                                            tGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCg                           <  2:5275333/150‑1 (MQ=255)

                                                                                                                            tGGATGGTGCGATGGCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAACCCGGGCg                           >  2:2647759/1‑150 (MQ=255)

                                                                                                                            tGGAAGGTGCGATGTCGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCAACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGAAAAATACCGGCTCTAACAAAATTTTGTCACACACCCAGAACTGCTAAAAACAGGGCg                           >  2:2203107/1‑150 (MQ=255)

                                                                                                                                   tGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGt                    <  1:2569919/150‑1 (MQ=255)

                                                                                                                                    gCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTg                   <  1:693099/150‑1 (MQ=255)

                                                                                                                                    gCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTg                   <  1:186108/150‑1 (MQ=255)

                                                                                                                                    gCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATAGTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTg                   <  2:3837403/150‑1 (MQ=255)

                                                                                                                                         ggCGAATAATATCGCAACCGGCGCAATTAACGTTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCATAACGGCTACAAACCGGGCGATCTGGTGAGTTa              <  1:719928/150‑1 (MQ=255)

                                                                                                                                         ggCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTa              <  1:4813792/150‑1 (MQ=255)

                                                                                                                                         ggCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTa              <  1:2122607/150‑1 (MQ=255)

                                                                                                                                          gCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTat             >  1:323603/1‑150 (MQ=255)

                                                                                                                                           cGAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatc            >  1:2010947/1‑150 (MQ=255)

                                                                                                                                            gAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCCTGCAGCAAAATACCGGCGCTAACAATATTGTGGCACCCACCCAGAACGGCTACACACCGGGCGATCTGGTGAGTTagca           >  2:867681/1‑150 (MQ=255)

                                                                                                                                            gAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACACACCGGGCGATCTGGTGAGTTatca           >  2:1971468/1‑150 (MQ=255)

                                                                                                                                            gAATAATATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatca           >  1:1182838/1‑150 (MQ=255)

                                                                                                                                             aataatATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatcaa          <  1:5992/150‑1 (MQ=255)

                                                                                                                                             aataatATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatcaa          >  1:1607748/1‑150 (MQ=255)

                                                                                                                                             aataatATAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGGTatcaa          >  2:2092687/1‑150 (MQ=255)

                                                                                                                                                aataTCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatcaaatc       >  1:3491789/1‑150 (MQ=255)

                                                                                                                                                aataTAGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatcaaatc       <  2:5115772/150‑1 (MQ=255)

ATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGA  >  CP009273/1128615‑1128913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`