Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,139,402 C→A 100% R141L (CGC→CTC)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,139,4020CA98.5% 1067.1 / 4.6 338R141L (CGC→CTC) rnefused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base A (146/185);  total (148/190)
Fisher's exact test for biased strand distribution p-value = 7.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 340 total aligned reads displayed.

TTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACA  >  CP009273/1139257‑1139548
                                                                                                                                                 |                                                                                                                                                  
 ttCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGaa                                                                                                                                               <  2:4206854/150‑1 (MQ=255)
       cGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCa                                                                                                                                         <  2:551254/150‑1 (MQ=255)
        ggAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAc                                                                                                                                        >  1:595867/1‑150 (MQ=255)
        ggAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAc                                                                                                                                        <  2:2400005/150‑1 (MQ=255)
          aaGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAccc                                                                                                                                      <  1:1557967/150‑1 (MQ=255)
            gCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgc                                                                                                                                    <  2:497155/150‑1 (MQ=255)
            gCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgc                                                                                                                                    >  1:853526/1‑150 (MQ=255)
               tAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgcgcg                                                                                                                                 >  2:626206/1‑150 (MQ=255)
                aaaTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgcgcgc                                                                                                                                >  2:4487341/1‑150 (MQ=255)
                  aTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCgg                                                                                                                              >  1:739544/1‑150 (MQ=255)
                    cccATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGgt                                                                                                                            >  1:2390812/1‑150 (MQ=255)
                     ccATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGAGCGGgtt                                                                                                                           >  1:999423/1‑150 (MQ=255)
                         tGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTc                                                                                                                       <  2:910864/150‑1 (MQ=255)
                         tGCAGCGCCTCAGCAGATGTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTc                                                                                                                       <  1:4502517/150‑1 (MQ=255)
                         ggCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTc                                                                                                                       <  2:4630103/149‑1 (MQ=255)
                          gCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCg                                                                                                                      >  1:2227717/1‑150 (MQ=255)
                          gCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCg                                                                                                                      >  1:4875283/1‑150 (MQ=255)
                            aGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGc                                                                                                                    <  1:1579565/150‑1 (MQ=255)
                            aGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGc                                                                                                                    <  1:3745706/150‑1 (MQ=255)
                              cgcCTCAGCAGATTTGACGACGCCAGCGGTGCGCACGATAAGCCCCATGACTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAACCGCGCGCGGGTTGTTCGGAAt                                                                                                                  >  2:2087722/1‑150 (MQ=255)
                                 cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAg                                                                                                               >  1:2514158/1‑150 (MQ=255)
                                    agcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAc                                                                                                            <  1:3121779/150‑1 (MQ=255)
                                     gcagATGTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAcc                                                                                                           <  2:4039810/150‑1 (MQ=255)
                                       agATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAg                                                                                                         <  2:2019373/150‑1 (MQ=255)
                                         aTTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGAt                                                                                                       <  2:2211480/150‑1 (MQ=255)
                                         aTTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGAt                                                                                                       <  2:1916907/150‑1 (MQ=255)
                                          tttGGCGTCGCGAGCGGTGCGCTCGATAAGACCCATGCCTTGCGGCTGTTCAAGGCTTGCCTGTGCTTCTTTTTATTCGGTACGGTCGTCGCCTTCGATTCGGAGAGATATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGAtt                                                                                                      <  2:3545242/150‑2 (MQ=255)
                                          tttGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATa                                                                                                      <  1:4994218/150‑1 (MQ=255)
                                           ttGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGATTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAg                                                                                                     <  1:870742/150‑1 (MQ=255)
                                            tGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGc                                                                                                    <  1:2676258/150‑1 (MQ=255)
                                               cGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAc                                                                                                 <  1:3705118/150‑1 (MQ=255)
                                                gACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAcc                                                                                                >  1:1043298/1‑150 (MQ=255)
                                                  cGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCg                                                                                              <  2:2509869/150‑1 (MQ=255)
                                                     cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCa                                                                                           <  2:748239/150‑1 (MQ=255)
                                                     cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCa                                                                                           >  2:4550565/1‑150 (MQ=255)
                                                     cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCa                                                                                           <  2:260130/150‑1 (MQ=255)
                                                         ggTGCTCACGATAAGCCCCATGCATTCCTGCAGTTCAAGGCTTGCCAGTTCTTCTTTTAATTCGGTAAGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGTCATCAGAACCATATAGATACCAGCCataat                                                                                       <  2:3419724/150‑5 (MQ=255)
                                                         ggTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACt                                                                                       >  2:1810519/1‑150 (MQ=255)
                                                                aCGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAg                                                                                <  2:4275330/150‑1 (MQ=255)
                                                                 cGATAAGCCCCATGCCTTCCGGCAGTTCTAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGACAGACTGATAAAgg                                                                               <  2:3039955/150‑1 (MQ=255)
                                                                 cGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCGTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATCGCTACCCGCCAGACAGATAATgg                                                                               >  2:1888698/1‑150 (MQ=255)
                                                                  gATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAggt                                                                              >  1:2004829/1‑150 (MQ=255)
                                                                  gATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAggt                                                                              >  2:2769225/1‑150 (MQ=255)
                                                                     aaGCCCCAAGCCTTCCTGCAGTTAAAGGCTTGCCAGAGCAACTTTTAATTCTGTACGGTCGTCGCCTACGATACGGAGAGAAATGCCACCCGCGCGCGTGTTGTTCGGCAACAGAACAAGAAAGCTACCCGCCAGACTGATAAAggtggt                                                                           >  2:2910891/1‑150 (MQ=255)
                                                                      aGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAGCCAGATAGCTACCCGCCAGACTGATAAAGGTGGtt                                                                          <  1:5075933/150‑1 (MQ=255)
                                                                       gCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTa                                                                         <  2:4811594/150‑1 (MQ=255)
                                                                         cccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAAt                                                                       >  2:4723585/1‑150 (MQ=255)
                                                                          ccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATg                                                                      >  1:2638980/1‑150 (MQ=255)
                                                                            aTGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGcc                                                                    >  1:4642467/1‑150 (MQ=255)
                                                                            aTGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGcc                                                                    <  2:3338391/150‑1 (MQ=255)
                                                                              gCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCcgc                                                                  <  2:1669057/150‑1 (MQ=255)
                                                                                cTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCgcgc                                                                >  1:5209163/1‑150 (MQ=255)
                                                                                  tCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCt                                                              >  2:430583/1‑150 (MQ=255)
                                                                                     ggCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttg                                                           <  2:4336231/150‑1 (MQ=255)
                                                                                     ggCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTACGGCATCAGAACCACATAGCTACCCGCCAGACTGATAAACGTGGTTAATGCCGCGCCttag                                                           >  1:2827094/1‑148 (MQ=255)
                                                                                       cAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgtt                                                         <  2:1400589/150‑1 (MQ=255)
                                                                                       cAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgtt                                                         <  1:4562410/150‑1 (MQ=255)
                                                                                       cAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgtt                                                         <  1:2303379/150‑1 (MQ=255)
                                                                                        aGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgttg                                                        <  1:2088311/150‑1 (MQ=255)
                                                                                         gTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGc                                                       >  1:3572919/1‑150 (MQ=255)
                                                                                                 cTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGctct                                               >  2:835266/1‑150 (MQ=255)
                                                                                                  ttGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtctt                                              <  1:528052/150‑1 (MQ=255)
                                                                                                  ttGCCAGTGCTTCTTTTAATTCGGTAAGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGAGGGTTGTTCGGCATCAGAACAAGATAGATACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtctt                                              >  1:2921860/1‑150 (MQ=255)
                                                                                                   tGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttc                                             <  1:128321/150‑1 (MQ=255)
                                                                                                   tGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttc                                             >  2:5121662/1‑150 (MQ=255)
                                                                                                    gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGACCCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                            <  2:1291283/150‑1 (MQ=255)
                                                                                                    gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                            <  2:714090/150‑1 (MQ=255)
                                                                                                    gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                            <  1:2965490/150‑1 (MQ=255)
                                                                                                     ccAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTcttctt                                           <  2:5076654/150‑1 (MQ=255)
                                                                                                      cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCttt                                          >  2:1991029/1‑150 (MQ=255)
                                                                                                      cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCttt                                          >  2:3046730/1‑150 (MQ=255)
                                                                                                      cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGACGCGCTCTTCttt                                          >  2:3881201/1‑150 (MQ=255)
                                                                                                         tGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATc                                       <  1:1400339/150‑1 (MQ=255)
                                                                                                         tGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCGCTTCTTTATc                                       >  1:4021090/1‑150 (MQ=255)
                                                                                                          gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCg                                      <  1:3926461/150‑1 (MQ=255)
                                                                                                          gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCg                                      <  1:3064622/150‑1 (MQ=255)
                                                                                                             tcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATc                                   >  1:273131/1‑150 (MQ=255)
                                                                                                             tcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATc                                   >  1:1195539/1‑150 (MQ=255)
                                                                                                               ttttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTg                                 <  2:1504979/150‑1 (MQ=255)
                                                                                                                  tAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAAc                              <  1:3279411/150‑1 (MQ=255)
                                                                                                                  tAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAAc                              <  1:3846688/150‑1 (MQ=255)
                                                                                                                   aaTTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACa                             >  2:4003397/1‑150 (MQ=255)
                                                                                                                   aaTTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACa                             >  2:3623309/1‑150 (MQ=255)
                                                                                                                      tCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATg                          <  1:1728974/150‑1 (MQ=255)
                                                                                                                         gTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACt                       >  2:1218020/1‑150 (MQ=255)
                                                                                                                             ggTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCt                   <  1:3438488/150‑1 (MQ=255)
                                                                                                                             ggTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCt                   <  2:3227845/150‑1 (MQ=255)
                                                                                                                             ggTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCt                   >  1:4288750/1‑150 (MQ=255)
                                                                                                                              gtcgtcGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTg                  <  1:6862/150‑1 (MQ=255)
                                                                                                                                   cgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCtt             >  2:2999827/1‑150 (MQ=255)
                                                                                                                                   cgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCtt             <  2:2482819/150‑1 (MQ=255)
                                                                                                                                     ccTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCa           <  2:4882645/150‑1 (MQ=255)
                                                                                                                                      cTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCAc          <  2:3799088/150‑1 (MQ=255)
                                                                                                                                      cTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCAc          <  2:5212405/150‑1 (MQ=255)
                                                                                                                                      cTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTGTATCGATCTGAACAATGACTTCCTGACCTTCAc          >  2:270604/1‑150 (MQ=255)
                                                                                                                                         cGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCa       >  1:19093/1‑150 (MQ=255)
                                                                                                                                          gATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCaa      >  2:1612560/1‑150 (MQ=255)
                                                                                                                                           aTACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAac     >  1:1501242/1‑150 (MQ=255)
                                                                                                                                           aTACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAac     >  1:4118775/1‑150 (MQ=255)
                                                                                                                                            tACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAaca    <  1:5096765/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACA  >  CP009273/1139257‑1139548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑