Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 104,561 G→T 100% A124S (GCG→TCG)  secM → regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273104,5610GT100.0% 1202.6 / NA 360A124S (GCG→TCG) secMregulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (220/140);  total (220/140)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 363 total aligned reads displayed.

AACTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTAACAA  >  CP009273/104414‑104707
                                                                                                                                                   |                                                                                                                                                  
   tATTCGGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctg                                                                                                                                               >  2:4196285/1‑150 (MQ=255)
   tATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctg                                                                                                                                               >  1:3875757/1‑150 (MQ=255)
    aTTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctgc                                                                                                                                              >  2:2302872/1‑150 (MQ=255)
       ccGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGa                                                                                                                                           >  2:2208534/1‑150 (MQ=255)
        cGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAc                                                                                                                                          >  2:1596235/1‑150 (MQ=255)
         gTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAcc                                                                                                                                         >  2:2077856/1‑150 (MQ=255)
          ttGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAccc                                                                                                                                        <  1:5033140/150‑1 (MQ=255)
          ttGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAccc                                                                                                                                        <  2:1599361/150‑1 (MQ=255)
           tGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCa                                                                                                                                       <  2:1719241/150‑1 (MQ=255)
            gATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAg                                                                                                                                      >  2:2149346/1‑150 (MQ=255)
            gATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAg                                                                                                                                      >  1:5109222/1‑150 (MQ=255)
             aTTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTTACCCAgg                                                                                                                                     >  1:2097043/1‑150 (MQ=255)
              ttACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGa                                                                                                                                    <  1:4650644/150‑1 (MQ=255)
               tACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGaa                                                                                                                                   >  1:1891864/1‑150 (MQ=255)
                aCTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAg                                                                                                                                  <  2:3808363/150‑1 (MQ=255)
                 cTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAgg                                                                                                                                 <  2:5139283/150‑1 (MQ=255)
                  tGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGc                                                                                                                                >  2:3232243/1‑150 (MQ=255)
                    gCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCAc                                                                                                                              <  1:2250469/150‑1 (MQ=255)
                      aTCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGCAGGCACGc                                                                                                                            >  2:4649039/1‑150 (MQ=255)
                      aTCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGc                                                                                                                            >  1:1039526/1‑150 (MQ=255)
                        cAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCg                                                                                                                          <  2:1531481/150‑1 (MQ=255)
                        cAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCg                                                                                                                          >  1:3640741/1‑150 (MQ=255)
                         aaCATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGt                                                                                                                         >  2:3964754/1‑150 (MQ=255)
                          aCATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTc                                                                                                                        <  2:1988395/150‑1 (MQ=255)
                           cATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCt                                                                                                                       <  2:3410064/150‑1 (MQ=255)
                              gCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaa                                                                                                                    >  1:1137465/1‑150 (MQ=255)
                              gCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaa                                                                                                                    >  1:2882220/1‑150 (MQ=255)
                               ccATTCGCACTGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGTCTGaaa                                                                                                                   >  2:1384931/1‑150 (MQ=255)
                               ccATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaa                                                                                                                   >  1:1682616/1‑150 (MQ=255)
                               ccATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaa                                                                                                                   >  1:3298510/1‑150 (MQ=255)
                                 aTTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGACAAg                                                                                                                 >  1:756364/1‑150 (MQ=255)
                                 aTTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAg                                                                                                                 >  2:2693950/1‑150 (MQ=255)
                                  ttCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaaa                                                                                                                  >  2:2841920/1‑148 (MQ=255)
                                    cGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCTCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGt                                                                                                              >  1:1374369/1‑150 (MQ=255)
                                     gCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGtt                                                                                                             >  1:2797175/1‑150 (MQ=255)
                                     gCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGtt                                                                                                             <  2:1277776/150‑1 (MQ=255)
                                      cACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTa                                                                                                            >  2:3304985/1‑150 (MQ=255)
                                       aaggaaaTCCGTCATCTATCTTTCGCAATGTCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTTCCTCTTCAGGCGAAACATCTTTCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTAt                                                                                                           >  2:5294767/6‑150 (MQ=255)
                                        cGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATc                                                                                                          >  2:5224284/1‑150 (MQ=255)
                                         ggTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCg                                                                                                         >  1:375591/1‑150 (MQ=255)
                                            aaTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                      <  1:2687360/150‑1 (MQ=255)
                                            aaTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                      >  2:1123970/1‑150 (MQ=255)
                                            aaTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                      <  1:305633/150‑1 (MQ=255)
                                             aTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAtt                                                                                                     <  2:2927203/150‑1 (MQ=255)
                                             aTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAtt                                                                                                     <  1:3156074/150‑1 (MQ=255)
                                             aTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAtt                                                                                                     <  2:3086410/150‑1 (MQ=255)
                                              tCCGTCATCTTTCTTTCGCAATGGCACCGCACACACTGCCCGTTGCTGAAGTCTCTTTGCCTCTTCCGGCGCAACATCTGGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAACAGGGTTATCGccgtg                                                                                                    >  2:2965966/1‑146 (MQ=255)
                                              tCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTg                                                                                                    <  2:4050290/150‑1 (MQ=255)
                                               ccGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGa                                                                                                   <  2:2533802/150‑1 (MQ=255)
                                               ccGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGa                                                                                                   <  1:4530490/150‑1 (MQ=255)
                                                cGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGAt                                                                                                  <  2:1039866/150‑1 (MQ=255)
                                                cGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGAt                                                                                                  <  1:342671/150‑1 (MQ=255)
                                                 gTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGAATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGAtt                                                                                                 >  1:1627038/1‑150 (MQ=255)
                                                    aTCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATg                                                                                              <  1:427807/150‑1 (MQ=255)
                                                    aTCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATg                                                                                              >  2:4632632/1‑150 (MQ=255)
                                                      ctttctttCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATgcg                                                                                            >  2:3909385/1‑150 (MQ=255)
                                                          ctttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCAtt                                                                                        >  1:4878201/1‑150 (MQ=255)
                                                                cAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTAccc                                                                                  >  2:4999644/1‑150 (MQ=255)
                                                                    ggCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCcaca                                                                              >  2:4079918/1‑150 (MQ=255)
                                                                    ggCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCcaca                                                                              >  1:2380888/1‑150 (MQ=255)
                                                                       aCCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGc                                                                           <  1:3435485/150‑1 (MQ=255)
                                                                         cGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaa                                                                         <  1:3987246/150‑1 (MQ=255)
                                                                          gCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaaa                                                                        >  1:5284241/1‑150 (MQ=255)
                                                                           cAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaaaa                                                                       >  2:3629704/1‑150 (MQ=255)
                                                                           cAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaaaa                                                                       >  2:3202217/1‑150 (MQ=255)
                                                                            aaaCACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAAt                                                                      <  1:2449708/150‑1 (MQ=255)
                                                                            aaaCACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAAt                                                                      >  2:2783880/1‑150 (MQ=255)
                                                                              acacTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTc                                                                    >  1:2631724/1‑150 (MQ=255)
                                                                              acacTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTc                                                                    <  2:1361210/150‑1 (MQ=255)
                                                                                    cccGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACg                                                              >  1:3791339/1‑150 (MQ=255)
                                                                                          gCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTc                                                        >  2:2985970/1‑150 (MQ=255)
                                                                                           cTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCt                                                       >  2:1837228/1‑150 (MQ=255)
                                                                                              aagaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGa                                                    >  1:3118064/1‑150 (MQ=255)
                                                                                               agaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGAt                                                   <  2:2321099/150‑1 (MQ=255)
                                                                                               agaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGAt                                                   >  1:5006850/1‑150 (MQ=255)
                                                                                               agaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGAt                                                   >  2:4810421/1‑150 (MQ=255)
                                                                                               agaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGAt                                                   >  1:413641/1‑150 (MQ=255)
                                                                                                 aaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATaa                                                 <  2:682941/150‑1 (MQ=255)
                                                                                                    cTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGcc                                              >  1:619617/1‑150 (MQ=255)
                                                                                                       tGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAgg                                           >  2:4148347/1‑150 (MQ=255)
                                                                                                           tctTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCa                                       <  2:2379843/150‑1 (MQ=255)
                                                                                                                aGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCa                                  >  1:2555670/1‑150 (MQ=255)
                                                                                                                   cgcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATcc                               <  2:2583648/150‑1 (MQ=255)
                                                                                                                    gcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCg                              <  2:2413087/150‑1 (MQ=255)
                                                                                                                     cAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGt                             >  2:4262767/1‑150 (MQ=255)
                                                                                                                     cAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGt                             >  2:192623/1‑150 (MQ=255)
                                                                                                                      aaCATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTg                            >  1:2719116/1‑150 (MQ=255)
                                                                                                                       aCATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCATGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGc                           >  1:682069/1‑150 (MQ=255)
                                                                                                                        cATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCt                          <  2:3531040/150‑1 (MQ=255)
                                                                                                                        cATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCt                          >  2:830944/1‑150 (MQ=255)
                                                                                                                         aTCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTg                         >  2:4695840/1‑150 (MQ=255)
                                                                                                                          tCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTgg                        <  2:3685380/150‑1 (MQ=255)
                                                                                                                               cATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTc                   <  1:1439184/150‑1 (MQ=255)
                                                                                                                               cATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTc                   >  1:834247/1‑150 (MQ=255)
                                                                                                                                   aCTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACg               >  1:2147613/1‑150 (MQ=255)
                                                                                                                                    cTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGc              >  1:1940538/1‑150 (MQ=255)
                                                                                                                                        aTACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCa          >  1:2267852/1‑150 (MQ=255)
                                                                                                                                         tACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCAc         >  1:949879/1‑150 (MQ=255)
                                                                                                                                           cGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCt       <  2:4561803/150‑1 (MQ=255)
                                                                                                                                               cAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTaaca   >  2:3053043/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTAACAA  >  CP009273/104414‑104707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑