Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,156,310 G→T 100% S233* (TCA→TAA)  fhuE ← ferric‑rhodotorulic acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,156,3100GT100.0% 1009.9 / ‑6.7 308S233* (TCA→TAA) fhuEferric‑rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (147/160);  minor base A (1/0);  total (148/160)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 308 total aligned reads displayed.

TTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCG  >  CP009273/1156163‑1156456
                                                                                                                                                   |                                                                                                                                                  
   cAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCg                                                                                                                                               <  2:687549/150‑1 (MQ=255)
      cgcgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTa                                                                                                                                            <  1:5096738/150‑1 (MQ=255)
      cgcgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTa                                                                                                                                            >  1:1752331/1‑150 (MQ=255)
      cgcgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTa                                                                                                                                            >  2:2418678/1‑150 (MQ=255)
        cgGTAAACCGCCCCTGGTAGGACTATTAACATCACTGCGCTGATATTCGTCACCGGCTGAAAGCGTCGTAAGAGCGCCTAAATCAGCATCGACACTGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTAtt                                                                                                                                          <  1:3390249/150‑1 (MQ=255)
        cgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTAtt                                                                                                                                          >  2:2038103/1‑150 (MQ=255)
          gTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCt                                                                                                                                        <  2:3370434/150‑1 (MQ=255)
          gTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCt                                                                                                                                        <  1:1597149/150‑1 (MQ=255)
          gTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCt                                                                                                                                        <  2:619284/150‑1 (MQ=255)
          gTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCt                                                                                                                                        <  1:4467237/150‑1 (MQ=255)
            aaaCCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTgg                                                                                                                                      <  1:1431938/150‑1 (MQ=255)
                  ccccAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccg                                                                                                                                >  2:3912965/1‑150 (MQ=255)
                  ccccAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccg                                                                                                                                >  2:2556790/1‑150 (MQ=255)
                   cccAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccgc                                                                                                                               <  1:4982048/150‑1 (MQ=255)
                      aGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGa                                                                                                                            >  2:2475621/1‑150 (MQ=255)
                       ggTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGAc                                                                                                                           <  1:4519011/150‑1 (MQ=255)
                         tAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACac                                                                                                                         >  1:3175249/1‑149 (MQ=255)
                             gCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcg                                                                                                                     >  1:1070169/1‑150 (MQ=255)
                               tATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcg                                                                                                                   >  2:2775866/1‑150 (MQ=255)
                                aTTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcgc                                                                                                                  >  1:4733893/1‑150 (MQ=255)
                                    aCATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCgg                                                                                                              <  2:2931877/150‑1 (MQ=255)
                                        cacggcgcTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGAttt                                                                                                          <  1:2267144/146‑1 (MQ=255)
                                         aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGAtttt                                                                                                         >  1:4437444/1‑150 (MQ=255)
                                           tGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTAc                                                                                                       <  1:5149764/150‑1 (MQ=255)
                                           tGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTAc                                                                                                       <  2:4339987/150‑1 (MQ=255)
                                                 gATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCtt                                                                                                 <  1:901401/150‑1 (MQ=255)
                                                  atatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTc                                                                                                >  1:2084357/1‑150 (MQ=255)
                                                  atatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTc                                                                                                <  2:457376/150‑1 (MQ=255)
                                                  atatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTc                                                                                                <  2:1964496/150‑1 (MQ=255)
                                                  atatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTc                                                                                                <  2:5227326/150‑1 (MQ=255)
                                                    atTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGTTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCgg                                                                                              >  1:660246/1‑150 (MQ=255)
                                                    atTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCgg                                                                                              >  1:435352/1‑150 (MQ=255)
                                                    atTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCgg                                                                                              >  1:3024396/1‑150 (MQ=255)
                                                    atTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCgg                                                                                              >  1:3945323/1‑150 (MQ=255)
                                                        gTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAg                                                                                          <  1:1138072/150‑1 (MQ=255)
                                                          aaCCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTg                                                                                        >  1:2498351/1‑150 (MQ=255)
                                                          aaCCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTg                                                                                        >  2:4202435/1‑150 (MQ=255)
                                                          aaCCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTg                                                                                        <  1:406259/150‑1 (MQ=255)
                                                               gCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCtt                                                                                   <  2:3808775/150‑1 (MQ=255)
                                                               gCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCtt                                                                                   <  2:1138136/150‑1 (MQ=255)
                                                                cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCttt                                                                                  <  1:2353902/150‑1 (MQ=255)
                                                                cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCttt                                                                                  >  2:3418307/1‑150 (MQ=255)
                                                                cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCttt                                                                                  >  2:4808132/1‑150 (MQ=255)
                                                                 tGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTg                                                                                 <  1:4292406/150‑1 (MQ=255)
                                                                 tGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTg                                                                                 >  2:4116072/1‑150 (MQ=255)
                                                                  gAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGt                                                                                >  1:1348355/1‑150 (MQ=255)
                                                                  gAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGt                                                                                <  2:2638716/150‑1 (MQ=255)
                                                                   aaaGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTa                                                                               <  1:4863106/150‑1 (MQ=255)
                                                                   aaaGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTa                                                                               >  2:3722294/1‑150 (MQ=255)
                                                                    aaGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTaa                                                                              <  2:2378662/150‑1 (MQ=255)
                                                                             aaGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCcac                                                                     <  1:1252525/150‑1 (MQ=255)
                                                                             aaGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCcac                                                                     <  1:3593730/150‑1 (MQ=255)
                                                                               gATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACAt                                                                   <  2:1463308/150‑1 (MQ=255)
                                                                                  cGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATAcc                                                                <  2:2836151/150‑1 (MQ=255)
                                                                                  cGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATAcc                                                                <  2:10537/150‑1 (MQ=255)
                                                                                  cGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTGATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATAcc                                                                <  1:1019452/150‑1 (MQ=255)
                                                                                        aaTCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCtt                                                          >  2:2134230/1‑150 (MQ=255)
                                                                                        aaTCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCtt                                                          <  2:718713/150‑1 (MQ=255)
                                                                                              cATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTcc                                                    >  2:3115256/1‑150 (MQ=255)
                                                                                              cATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTcc                                                    <  1:3890280/150‑1 (MQ=255)
                                                                                                 cGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGc                                                 >  2:1724784/1‑150 (MQ=255)
                                                                                                  gACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCt                                                <  1:36882/150‑1 (MQ=255)
                                                                                                  gACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGATCTTTGTTCCAGCt                                                >  2:4720945/1‑150 (MQ=255)
                                                                                                    cAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTAc                                              >  2:133321/1‑150 (MQ=255)
                                                                                                    cAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTAc                                              >  2:4473467/1‑150 (MQ=255)
                                                                                                     aaTGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTAcc                                             <  1:2896199/150‑1 (MQ=255)
                                                                                                      aTGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGATCTTTGTTCCAGCTACCg                                            >  1:2957990/1‑150 (MQ=255)
                                                                                                        gCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTa                                          <  1:5324468/150‑1 (MQ=255)
                                                                                                        gCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTa                                          >  2:5136391/1‑150 (MQ=255)
                                                                                                          ccGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTAtt                                        <  2:125927/150‑1 (MQ=255)
                                                                                                          ccGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTAtt                                        <  2:3756860/150‑1 (MQ=255)
                                                                                                          ccGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTAtt                                        <  2:3628422/150‑1 (MQ=255)
                                                                                                            gagaAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTcc                                      <  1:2774069/150‑1 (MQ=255)
                                                                                                             agaagaAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccg                                     <  2:2590284/150‑1 (MQ=255)
                                                                                                             agaagaAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccg                                     <  1:3099208/150‑1 (MQ=255)
                                                                                                               aagaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgcc                                   >  1:1653731/1‑150 (MQ=255)
                                                                                                                agaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgccg                                  >  1:4125249/1‑150 (MQ=255)
                                                                                                                agaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgccg                                  >  2:4073770/1‑150 (MQ=255)
                                                                                                                agaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTGGTTCCAGCTACCGTATTccgccg                                  <  2:4926023/150‑1 (MQ=255)
                                                                                                                  aagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCgag                                >  1:1212039/1‑150 (MQ=255)
                                                                                                                    ggTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGAc                              <  2:1585624/150‑1 (MQ=255)
                                                                                                                     gTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACa                             <  1:2841545/150‑1 (MQ=255)
                                                                                                                          ttCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGcc                        >  1:1856054/1‑150 (MQ=255)
                                                                                                                          ttCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGcc                        >  1:2621387/1‑150 (MQ=255)
                                                                                                                           tCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTTAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCt                       >  2:3801393/1‑150 (MQ=255)
                                                                                                                            cACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCtt                      <  1:264118/150‑1 (MQ=255)
                                                                                                                             aCTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCttt                     <  1:4186176/150‑1 (MQ=255)
                                                                                                                                        ggTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGAc          >  1:453387/1‑150 (MQ=255)
                                                                                                                                        ggTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGAc          <  1:1736213/150‑1 (MQ=255)
                                                                                                                                         gTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACt         <  1:2165325/150‑1 (MQ=255)
                                                                                                                                         gTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACt         <  1:787621/150‑1 (MQ=255)
                                                                                                                                         gTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACt         >  1:1839997/1‑150 (MQ=255)
                                                                                                                                         gTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACt         >  1:3238758/1‑150 (MQ=255)
                                                                                                                                           ccAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTgg       <  2:5091837/150‑1 (MQ=255)
                                                                                                                                            cAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGt      <  1:3920733/150‑1 (MQ=255)
                                                                                                                                            cAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGt      >  2:4998071/1‑150 (MQ=255)
                                                                                                                                             aGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTc     >  1:3649809/1‑150 (MQ=255)
                                                                                                                                              gCCATTAGTCTTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGATACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcg    <  2:1853198/150‑1 (MQ=255)
                                                                                                                                               ccATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcgc   >  1:3449106/1‑150 (MQ=255)
                                                                                                                                                cATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcgcg  <  2:2084387/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCG  >  CP009273/1156163‑1156456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑