Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,163,251 A→T 5.2% Q66L (CAG→CTG)  ycfJ → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,163,2510AT 5.2% 891.1 / 27.1 287Q66L (CAG→CTG) ycfJuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (127/145);  new base T (9/6);  total (136/151)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 293 total aligned reads displayed.

GGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTC  >  CP009273/1163105‑1163400
                                                                                                                                                  |                                                                                                                                                     
 ggCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGAt                                                                                                                                                   <  1:5038663/150‑1 (MQ=255)
  gCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATg                                                                                                                                                  <  1:4889184/150‑1 (MQ=255)
   cGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGa                                                                                                                                                 <  1:1032047/150‑1 (MQ=255)
    gTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaa                                                                                                                                                >  2:4003515/1‑150 (MQ=255)
        cGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCNGGATGAAAATc                                                                                                                                            <  1:2765238/150‑1 (MQ=255)
         ggCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCg                                                                                                                                           <  2:5210106/150‑1 (MQ=255)
             gTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCTGGATGAAAATCGCAtt                                                                                                                                       <  2:2830625/150‑1 (MQ=255)
              tGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTa                                                                                                                                      <  2:4145812/150‑1 (MQ=255)
               ggCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTAc                                                                                                                                     <  1:386791/150‑1 (MQ=255)
                gCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTAcc                                                                                                                                    >  1:2371913/1‑150 (MQ=255)
                gCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTAcc                                                                                                                                    <  2:1957784/150‑1 (MQ=255)
                 ccAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCACTCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCg                                                                                                                                   <  2:3551872/150‑1 (MQ=255)
                  cAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCgg                                                                                                                                  <  1:1278585/150‑1 (MQ=255)
                       tGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCgg                                                                                                                             >  2:4356058/1‑150 (MQ=255)
                        gAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGt                                                                                                                            <  1:2322081/150‑1 (MQ=255)
                        gAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGt                                                                                                                            >  2:125202/1‑150 (MQ=255)
                            gtgtTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTc                                                                                                                        <  1:3101510/150‑1 (MQ=255)
                             tgtTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCg                                                                                                                       >  1:4333800/1‑150 (MQ=255)
                             tgtTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCg                                                                                                                       <  2:2483268/150‑1 (MQ=255)
                                    aCGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGt                                                                                                                <  1:3791589/150‑1 (MQ=255)
                                    aCGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGt                                                                                                                >  2:3885361/1‑150 (MQ=255)
                                      ggggCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTg                                                                                                              >  1:523546/1‑150 (MQ=255)
                                         gCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTg                                                                                                           <  1:1329358/150‑1 (MQ=255)
                                          cccGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTgg                                                                                                          <  1:4736190/150‑1 (MQ=255)
                                          cccGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTgg                                                                                                          <  1:2442864/150‑1 (MQ=255)
                                           ccGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTggc                                                                                                         >  1:44360/1‑150 (MQ=255)
                                            cGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTggcg                                                                                                        >  2:899243/1‑150 (MQ=255)
                                             gCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTggcgg                                                                                                       <  2:5258185/150‑1 (MQ=255)
                                              cAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTggcggc                                                                                                      >  2:1983002/1‑150 (MQ=255)
                                               aaTACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGgcggcg                                                                                                     >  2:4014258/1‑150 (MQ=255)
                                               aaTACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGgcggcg                                                                                                     >  2:649410/1‑150 (MQ=255)
                                                  aCGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGa                                                                                                  <  1:2098009/150‑1 (MQ=255)
                                                     cTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAg                                                                                               >  1:2266919/1‑150 (MQ=255)
                                                     cTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAg                                                                                               <  2:2378332/150‑1 (MQ=255)
                                                      tCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAgg                                                                                              >  1:2137384/1‑150 (MQ=255)
                                                      tCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAgg                                                                                              <  2:4408912/150‑1 (MQ=255)
                                                      tCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAgg                                                                                              <  2:735924/150‑1 (MQ=255)
                                                       cAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAggg                                                                                             <  1:1468147/150‑1 (MQ=255)
                                                           ttgttTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATc                                                                                         <  2:2082898/150‑1 (MQ=255)
                                                            tgttTCTGCAACCCCAATCAAGGTAAAGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCTTTACCGGGTCGGTGCTCAGCGCTGTTGCTGGCGGCGTGATATGGCATCa                                                                                        <  1:1795288/150‑1 (MQ=255)
                                                             gttTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAg                                                                                       >  1:63239/1‑150 (MQ=255)
                                                             gttTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAg                                                                                       <  1:2005590/150‑1 (MQ=255)
                                                              tttCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGt                                                                                      >  1:4565408/1‑150 (MQ=255)
                                                              tttCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGt                                                                                      <  1:3837598/150‑1 (MQ=255)
                                                               ttCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGtt                                                                                     <  1:4699524/150‑1 (MQ=255)
                                                               ttCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGtt                                                                                     >  2:220609/1‑150 (MQ=255)
                                                                 cTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTtg                                                                                   <  1:2659917/150‑1 (MQ=255)
                                                                 cTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCAGCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTtg                                                                                   <  2:122097/150‑1 (MQ=255)
                                                                  tGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTtgg                                                                                  >  2:1517087/1‑150 (MQ=255)
                                                                       ccccAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTtggtggtg                                                                             >  2:3256879/1‑150 (MQ=255)
                                                                        cccAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCTGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTtggtggtgg                                                                            <  2:3708664/150‑1 (MQ=255)
                                                                         ccAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTggtggtggt                                                                           >  2:3286058/1‑150 (MQ=255)
                                                                          cAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCTGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTc                                                                          >  1:859533/1‑150 (MQ=255)
                                                                          cAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTc                                                                          <  1:4841185/150‑1 (MQ=255)
                                                                          cAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTc                                                                          <  2:4938537/150‑1 (MQ=255)
                                                                            aTCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTcgc                                                                        <  2:4801548/150‑1 (MQ=255)
                                                                            aTCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTcgc                                                                        <  2:4661918/150‑1 (MQ=255)
                                                                                 ggAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTACTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTaa                                                                   <  2:2728054/150‑1 (MQ=255)
                                                                                    aaCGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGa                                                                >  1:3633162/1‑150 (MQ=255)
                                                                                     aCGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGAt                                                               <  1:33599/150‑1 (MQ=255)
                                                                                     aCGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGAt                                                               <  1:1440895/150‑1 (MQ=255)
                                                                                     aCGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGAt                                                               <  1:33737/150‑1 (MQ=255)
                                                                                      cGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATg                                                              <  2:1785635/150‑1 (MQ=255)
                                                                                       ggTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGt                                                             <  2:3837917/150‑1 (MQ=255)
                                                                                       ggTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGt                                                             <  1:4228407/150‑1 (MQ=255)
                                                                                        gTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTc                                                            <  2:4330985/150‑1 (MQ=255)
                                                                                        gTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTc                                                            >  2:3014979/1‑150 (MQ=255)
                                                                                          tAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGc                                                          >  2:1710573/1‑150 (MQ=255)
                                                                                                     gTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTgg                                               >  2:4732864/1‑150 (MQ=255)
                                                                                                     gTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGAGCTCGGCGCAGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTgg                                               >  1:5140707/1‑150 (MQ=255)
                                                                                                       cAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTgggg                                             >  1:1210053/1‑150 (MQ=255)
                                                                                                        aGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTggggg                                            >  2:1929858/1‑150 (MQ=255)
                                                                                                        aGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTggggg                                            >  2:5033776/1‑150 (MQ=255)
                                                                                                         ggAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGgc                                           >  2:3754/1‑150 (MQ=255)
                                                                                                            gtgtCGCAACGTCACAGTGACCCATCGTCGACCGATGCTGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCt                                        <  1:4016116/150‑1 (MQ=255)
                                                                                                              gtCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTgg                                      <  1:125143/150‑1 (MQ=255)
                                                                                                                 gCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGggtg                                   >  1:3533926/1‑150 (MQ=255)
                                                                                                                  cAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGggtgg                                  >  1:781396/1‑150 (MQ=255)
                                                                                                                    aCGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGgtggtg                                <  1:2317071/150‑1 (MQ=255)
                                                                                                                     cGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGtggtgg                               <  1:2901774/150‑1 (MQ=255)
                                                                                                                     cGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGtggtgg                               >  2:1442230/1‑150 (MQ=255)
                                                                                                                     cGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGtggtgg                               >  1:1850166/1‑150 (MQ=255)
                                                                                                                      gTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGa                              >  2:2151324/1‑150 (MQ=255)
                                                                                                                        cacaGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGata                            >  2:564947/1‑150 (MQ=255)
                                                                                                                        cacaGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGata                            >  2:3620010/1‑150 (MQ=255)
                                                                                                                         acaGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGatat                           <  2:4475924/150‑1 (MQ=255)
                                                                                                                            gTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGcc                        <  1:4626066/150‑1 (MQ=255)
                                                                                                                              gACCCATCGTCGACCGGTGCTGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCgg                      >  2:1660958/1‑150 (MQ=255)
                                                                                                                               aCCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGt                     >  2:2605158/1‑150 (MQ=255)
                                                                                                                                cccATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTa                    <  2:3425914/150‑1 (MQ=255)
                                                                                                                                     cgtcgACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAg               <  2:3289903/150‑1 (MQ=255)
                                                                                                                                      gtcgACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGa              <  2:769600/150‑1 (MQ=255)
                                                                                                                                           ccGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAg         <  1:1669203/150‑1 (MQ=255)
                                                                                                                                             ggTGGAGGATGTAAATCGGATTTGGGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAggg       <  2:4463778/150‑1 (MQ=255)
                                                                                                                                                gCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGctc    <  1:2254643/150‑1 (MQ=255)
                                                                                                                                                gCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCGGGGTGGTGGATATGCCGGTAACCAGATCCAGGGctc    >  1:3722591/1‑150 (MQ=255)
                                                                                                                                                 cAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGctct   <  2:3202946/150‑1 (MQ=255)
                                                                                                                                                 cAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGctct   <  2:1144395/150‑1 (MQ=255)
                                                                                                                                                  aGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTACAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGctctc  >  1:1547899/1‑150 (MQ=255)
                                                                                                                                                  aGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGctctc  <  1:3178585/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTC  >  CP009273/1163105‑1163400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑