BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,249,716	G→T	61.7%	R565S (CGC→AGC)	ycgV ←	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,249,716	0	G	T	61.7%	109.7 / inf	272	R565S (CGC→AGC)	ycgV	putative adhesin
Reads supporting (aligned to +/- strand): ref base G (53/51); new base T (80/88); total (133/139)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 275 total aligned reads displayed.

AGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATG  >  CP009273/1249568‑1249865

aGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCgg                                                                                                                                                      <  2:2746000/150‑1 (MQ=255)

  cTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGAt                                                                                                                                                    >  2:3074841/1‑150 (MQ=255)

  cTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGAt                                                                                                                                                    >  2:1849243/1‑150 (MQ=255)

   tCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATa                                                                                                                                                   >  1:5043302/1‑150 (MQ=255)

    cccAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAg                                                                                                                                                  <  2:1239985/150‑1 (MQ=255)

         ttAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAAt                                                                                                                                             <  1:2868756/150‑1 (MQ=255)

          tAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGTACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATa                                                                                                                                            >  2:4464936/1‑150 (MQ=255)

          tAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATa                                                                                                                                            >  1:1416272/1‑150 (MQ=255)

           aGTTCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGACGTCAGTGGTTTTTACCACTGTCAGAACTACATTTCCCGATGTGGCCTCGCTGCCCTGGTTGCTGATAGACAATAc                                                                                                                                           >  2:5173185/1‑150 (MQ=255)

               ccATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGa                                                                                                                                       >  2:2046424/1‑150 (MQ=255)

               ccATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGa                                                                                                                                       >  1:2360650/1‑150 (MQ=255)

                cATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTTGTTGCGTATAGCCAATACGTGAt                                                                                                                                      <  2:4495158/150‑1 (MQ=255)

                 aTTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGAtt                                                                                                                                     <  2:3197294/150‑1 (MQ=255)

                   ttttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTAc                                                                                                                                   <  2:3425850/150‑1 (MQ=255)

                    tttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTAcc                                                                                                                                  <  1:3922429/150‑1 (MQ=255)

                     ttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCa                                                                                                                                 <  1:1772501/150‑1 (MQ=255)

                      tACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAg                                                                                                                                <  1:239475/150‑1 (MQ=255)

                         gCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCCGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCac                                                                                                                             <  1:4222026/150‑1 (MQ=255)

                            caTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCactac                                                                                                                          <  1:4805899/150‑1 (MQ=255)

                             aTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCactact                                                                                                                         >  2:1292593/1‑150 (MQ=255)

                             aTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCactact                                                                                                                         >  2:3759704/1‑150 (MQ=255)

                               cGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCATTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACtcctcc                                                                                                                       >  2:3804091/1‑150 (MQ=255)

                                gTACAGATATCCCCCCTACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTccc                                                                                                                      <  2:4408755/150‑1 (MQ=255)

                                   cAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCt                                                                                                                   >  1:2499129/1‑150 (MQ=255)

                                    aGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                                  >  1:1577273/1‑150 (MQ=255)

                                    aGATATCCACCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCGGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                                  <  2:2222217/150‑1 (MQ=255)

                                      atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGat                                                                                                                <  2:1104182/150‑1 (MQ=255)

                                      atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGat                                                                                                                <  1:1925141/150‑1 (MQ=255)

                                       tatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGata                                                                                                               >  2:4058186/1‑150 (MQ=255)

                                        atCCCCCCAACTCAACCTGAGTAGACGCGCTGATCGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGTACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATTGCCAATACGTGATTAACAGCACTACTCCCGCTGatat                                                                                                              <  1:2612552/150‑1 (MQ=255)

                                          ccccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                            <  2:5085717/150‑1 (MQ=255)

                                            ccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCaa                                                                                                          <  2:3974570/150‑1 (MQ=255)

                                             cccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAAt                                                                                                         >  2:2579082/1‑150 (MQ=255)

                                              ccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATa                                                                                                        <  1:3480139/150‑1 (MQ=255)

                                               cAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATaa                                                                                                       >  2:2999298/1‑150 (MQ=255)

                                               cAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATaa                                                                                                       <  1:2429893/150‑1 (MQ=255)

                                                 aCTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAAt                                                                                                     >  1:4866238/1‑150 (MQ=255)

                                                  cTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATc                                                                                                    <  1:4060965/150‑1 (MQ=255)

                                                   tCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATcc                                                                                                   <  1:8648/150‑1 (MQ=255)

                                                   tCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATcc                                                                                                   >  1:2368451/1‑150 (MQ=255)

                                                    cAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATccc                                                                                                  >  1:5204226/1‑150 (MQ=255)

                                                     aaCCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATcccc                                                                                                 >  2:3268479/1‑150 (MQ=255)

                                                      aCCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCt                                                                                                <  1:1609273/150‑1 (MQ=255)

                                                       ccTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCtt                                                                                               >  1:3692102/1‑150 (MQ=255)

                                                           agaagaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttatt                                                                                           >  2:5093960/1‑150 (MQ=255)

                                                              agaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttattatt                                                                                        <  2:2322160/150‑1 (MQ=255)

                                                               gaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttattatta                                                                                       >  1:3698418/1‑150 (MQ=255)

                                                                 cgcgCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAAc                                                                                     <  2:2497179/150‑1 (MQ=255)

                                                                  gcgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACg                                                                                    <  2:485229/150‑1 (MQ=255)

                                                                   cgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGc                                                                                   >  2:2952624/1‑150 (MQ=255)

                                                                   cgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGc                                                                                   >  2:1514561/1‑150 (MQ=255)

                                                                    gcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGcc                                                                                  <  1:3762973/150‑1 (MQ=255)

                                                                     cTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGAGATTCAATAAATCCCCTTTATTATTAACGCCa                                                                                 >  2:133231/1‑150 (MQ=255)

                                                                      tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                                <  1:2994353/150‑1 (MQ=255)

                                                                       gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                               <  1:966674/150‑1 (MQ=255)

                                                                         aCGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAc                                                                             >  1:1049896/1‑150 (MQ=255)

                                                                           gAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAccc                                                                           >  1:2496167/1‑150 (MQ=255)

                                                                           gAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAccc                                                                           >  2:3071044/1‑150 (MQ=255)

                                                                           gAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAccc                                                                           >  1:4375492/1‑150 (MQ=255)

                                                                                   gcCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaac                                                                   <  2:715614/150‑1 (MQ=255)

                                                                                   gcCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaac                                                                   <  1:533796/150‑1 (MQ=255)

                                                                                     cGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa                                                                 >  1:5139907/1‑150 (MQ=255)

                                                                                     cGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGAGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATAACCCTCGCcaacaa                                                                 >  2:536049/1‑150 (MQ=255)

                                                                                      gTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGCGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCaacaac                                                                <  2:5139938/150‑1 (MQ=255)

                                                                                      gTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCaacaac                                                                >  1:4132907/1‑150 (MQ=255)

                                                                                         aGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATc                                                             >  2:4699253/1‑150 (MQ=255)

                                                                                           tGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAg                                                           >  2:5024250/1‑150 (MQ=255)

                                                                                            ggTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGc                                                          >  1:1008141/1‑150 (MQ=255)

                                                                                              tttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCAc                                                        <  1:4526067/150‑1 (MQ=255)

                                                                                               ttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACg                                                       >  1:1227950/1‑150 (MQ=255)

                                                                                               ttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACg                                                       >  1:2058259/1‑150 (MQ=255)

                                                                                                         gTCAGAACTTCCTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAg                                             >  1:2735160/1‑150 (MQ=255)

                                                                                                         gTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAg                                             >  1:4358999/1‑150 (MQ=255)

                                                                                                         gTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAg                                             >  1:4287689/1‑150 (MQ=255)

                                                                                                           cAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGt                                           <  1:1938530/150‑1 (MQ=255)

                                                                                                           cAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAGCAGCACGCATAATAAAGGt                                           >  2:2055558/1‑150 (MQ=255)

                                                                                                            aGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTa                                          >  2:2680859/1‑150 (MQ=255)

                                                                                                              aaCTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACt                                        <  2:1914304/150‑1 (MQ=255)

                                                                                                                    aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                                  <  2:5198645/150‑1 (MQ=255)

                                                                                                                    aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                                  >  2:2189179/1‑150 (MQ=255)

                                                                                                                    aTTTCCCGTTGGGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                                  <  1:4642636/150‑1 (MQ=255)

                                                                                                                        cccGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTc                              >  2:880665/1‑150 (MQ=255)

                                                                                                                        cccGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTc                              >  2:1728657/1‑150 (MQ=255)

                                                                                                                        cccGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTc                              <  2:2345559/150‑1 (MQ=255)

                                                                                                                          cGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAg                            <  2:1881435/150‑1 (MQ=255)

                                                                                                                          cGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAg                            >  1:5004058/1‑150 (MQ=255)

                                                                                                                          cGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAg                            >  1:4716801/1‑150 (MQ=255)

                                                                                                                              gtgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtc                        <  2:2532094/150‑1 (MQ=255)

                                                                                                                               tgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct                       <  2:3528158/150‑1 (MQ=255)

                                                                                                                                  ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa                    <  1:305064/150‑1 (MQ=255)

                                                                                                                                     cGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGt                 <  1:4264484/150‑1 (MQ=255)

                                                                                                                                      gCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt                <  2:1420232/150‑1 (MQ=255)

                                                                                                                                        tGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTa              >  2:634529/1‑150 (MQ=255)

                                                                                                                                        tGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTa              <  1:1243960/150‑1 (MQ=255)

                                                                                                                                         gCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTaa             >  2:2138147/1‑150 (MQ=255)

                                                                                                                                           ccTGGTTGCTGATAGACAATACGTGCATACCATCACTACTCCCGCAGATATTAAATAAATCCACTTTATTATTAACGCCATTACCCTCTCCAACTACATCAGCAAGCTTAATAAAGGTTCTGTTACCGCTCAGGTTCTCTTCGATTAAtg           <  2:853758/150‑1 (MQ=255)

                                                                                                                                             tGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg         <  1:573847/150‑1 (MQ=255)

                                                                                                                                             tGGTTGCTGATAGCCAATACGAGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg         >  1:2218237/1‑150 (MQ=255)

                                                                                                                                             tGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg         <  2:3626365/150‑1 (MQ=255)

                                                                                                                                              ggTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTgg        >  2:3417084/1‑150 (MQ=255)

AGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATG  >  CP009273/1249568‑1249865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`