Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,255,258 A→T 11.7% A334A (GCT→GCA)  dauA ← C4‑dicarboxylic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,255,2580AT11.7% 774.6 / 73.1 266A334A (GCT→GCA) dauAC4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (119/116);  new base T (18/13);  total (137/129)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 275 total aligned reads displayed.

AACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCAT  >  CP009273/1255109‑1255407
                                                                                                                                                     |                                                                                                                                                     
aaCAGCAGGGCTGCCATGGCGGATAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCa                                                                                                                                                       <  2:3463423/150‑1 (MQ=255)
aaCAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCa                                                                                                                                                       <  2:2071644/150‑1 (MQ=255)
 aCAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAg                                                                                                                                                      >  2:4198901/1‑150 (MQ=255)
  cagcagGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGATGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGc                                                                                                                                                     >  1:5014684/1‑150 (MQ=255)
       gggCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAg                                                                                                                                                >  1:4677930/1‑150 (MQ=255)
                tGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATac                                                                                                                                       <  1:4043274/150‑1 (MQ=255)
                 ggCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATacc                                                                                                                                      <  1:1693749/150‑1 (MQ=255)
                   cGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATaccac                                                                                                                                    <  2:45357/150‑1 (MQ=255)
                     gAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATAccacca                                                                                                                                  <  2:1124154/150‑1 (MQ=255)
                     gAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATAccacca                                                                                                                                  <  1:263748/150‑1 (MQ=255)
                       aaGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCaaa                                                                                                                                >  2:2227103/1‑150 (MQ=255)
                        aGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAg                                                                                                                               <  1:4816012/150‑1 (MQ=255)
                          cGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGaa                                                                                                                             <  1:4571533/150‑1 (MQ=255)
                           ggCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAAc                                                                                                                            <  2:531987/150‑1 (MQ=255)
                            gCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACg                                                                                                                           >  1:2799243/1‑150 (MQ=255)
                            gCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACg                                                                                                                           <  1:4230392/150‑1 (MQ=255)
                            gCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACg                                                                                                                           <  2:210187/150‑1 (MQ=255)
                              aGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGa                                                                                                                         >  1:3140653/1‑150 (MQ=255)
                              aGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGa                                                                                                                         <  1:3660490/150‑1 (MQ=255)
                              aGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGa                                                                                                                         >  1:1676762/1‑150 (MQ=255)
                               gCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCTGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                                                                                        <  2:1444575/150‑1 (MQ=255)
                               gCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                                                                                        <  2:3346281/150‑1 (MQ=255)
                                 cAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCg                                                                                                                      <  2:2849527/150‑1 (MQ=255)
                                  aGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCTGCTGTAGCGGTAATACCACCAAAGAACGGAGCGa                                                                                                                     >  2:3974425/1‑150 (MQ=255)
                                   ggAGAGCAGCGGTGCCAGTACCAGCAGGGCACGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGat                                                                                                                    >  1:2176046/1‑150 (MQ=255)
                                       agcagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGataata                                                                                                                <  1:1683658/150‑1 (MQ=255)
                                        gcagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAatat                                                                                                               <  1:1519790/150‑1 (MQ=255)
                                         cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATAtt                                                                                                              <  2:3786136/150‑1 (MQ=255)
                                         cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATAtt                                                                                                              <  2:2258897/150‑1 (MQ=255)
                                            cGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTccc                                                                                                           <  1:1766598/150‑1 (MQ=255)
                                            cGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTccc                                                                                                           <  1:713038/150‑1 (MQ=255)
                                            cGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTccc                                                                                                           <  1:2834364/150‑1 (MQ=255)
                                             ggTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTcccc                                                                                                          <  2:197601/150‑1 (MQ=255)
                                               tGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGAGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAg                                                                                                        <  1:67141/150‑1 (MQ=255)
                                                      aCCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCTGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGt                                                                                                 >  2:2745018/1‑150 (MQ=255)
                                                       ccAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGTGACGTTGCCCCGGCACGGACGTTAGCGGCAGATCGCGCGATGGCAGCTGTAGCGGTAATACCACCATAGAACGGAGCGATAATATTCCCCAGTCCCTGTc                                                                                                <  2:3165176/150‑1 (MQ=255)
                                                       ccAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTc                                                                                                <  2:1101049/150‑1 (MQ=255)
                                                         agcagGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCa                                                                                              >  2:1251131/1‑150 (MQ=255)
                                                           cagGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAAc                                                                                            <  2:4390646/150‑1 (MQ=255)
                                                            agGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAAcc                                                                                           <  1:3774336/150‑1 (MQ=255)
                                                             gggCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCa                                                                                          <  2:1825287/150‑1 (MQ=255)
                                                             gggCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCa                                                                                          <  2:4483769/150‑1 (MQ=255)
                                                                  aGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCTGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCg                                                                                     >  1:3891853/1‑150 (MQ=255)
                                                                  aGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCg                                                                                     <  2:2806263/150‑1 (MQ=255)
                                                                  aGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCg                                                                                     >  2:5216605/1‑150 (MQ=255)
                                                                      tAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGt                                                                                 >  2:3186823/1‑150 (MQ=255)
                                                                       aaCCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGtt                                                                                <  1:5095780/150‑1 (MQ=255)
                                                                         ccAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCg                                                                              <  1:1966121/150‑1 (MQ=255)
                                                                           aGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGcc                                                                            >  2:2448104/1‑150 (MQ=255)
                                                                            gAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCt                                                                           >  1:3314304/1‑150 (MQ=255)
                                                                             aaTAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt                                                                          >  1:4135895/1‑150 (MQ=255)
                                                                               tAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTtgt                                                                        >  1:982863/1‑150 (MQ=255)
                                                                                agagTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTtgtg                                                                       >  2:4544229/1‑150 (MQ=255)
                                                                                agagTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTtgtg                                                                       <  1:1073328/150‑1 (MQ=255)
                                                                                  agTGGATCCCCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGAGGGCTGCTGTAGCGGTAAGACCACCAAAGAACGGAGCGATAATAGTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGtt                                                                     <  2:883406/150‑1 (MQ=255)
                                                                                   gTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGttt                                                                    <  2:3221414/150‑1 (MQ=255)
                                                                                    tGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTc                                                                   >  2:5152974/1‑150 (MQ=255)
                                                                                    tGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTc                                                                   <  2:2683115/150‑1 (MQ=255)
                                                                                    tGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCGTGTGTTTc                                                                   >  2:5026816/1‑150 (MQ=255)
                                                                                     ggATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCTGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCg                                                                  >  2:1580420/1‑150 (MQ=255)
                                                                                      gATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGt                                                                 >  1:3596084/1‑150 (MQ=255)
                                                                                       aTCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTc                                                                >  2:3627751/1‑150 (MQ=255)
                                                                                        tCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTcc                                                               <  1:2380118/150‑1 (MQ=255)
                                                                                            cgccgAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGt                                                           <  2:3085341/150‑1 (MQ=255)
                                                                                               cgAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCAt                                                        <  2:3826758/150‑1 (MQ=255)
                                                                                                gagaTAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATa                                                       >  2:1649576/1‑150 (MQ=255)
                                                                                                gagaTAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATa                                                       >  1:1317735/1‑150 (MQ=255)
                                                                                                gagaTAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATa                                                       >  1:1564999/1‑150 (MQ=255)
                                                                                                 agaTAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATAc                                                      >  1:578963/1‑150 (MQ=255)
                                                                                                 agaTAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATAc                                                      >  1:2233394/1‑150 (MQ=255)
                                                                                                      ggggACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATcc                                                 <  1:488401/150‑1 (MQ=255)
                                                                                                      ggggACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATcc                                                 <  1:3165709/150‑1 (MQ=255)
                                                                                                       gggACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCa                                                <  2:749400/150‑1 (MQ=255)
                                                                                                           cGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGcac                                            >  1:1256976/1‑150 (MQ=255)
                                                                                                            gTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCTTCCCGGTCATACCATCCAGcacc                                           >  2:344596/1‑150 (MQ=255)
                                                                                                             ttGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCTGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGcacca                                          <  1:793741/150‑1 (MQ=255)
                                                                                                              tGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGcaccac                                         >  1:4451512/1‑150 (MQ=255)
                                                                                                              tGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTAGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGcaccac                                         >  2:1155134/1‑150 (MQ=255)
                                                                                                                ccccGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACgg                                       <  1:491472/150‑1 (MQ=255)
                                                                                                                       aCGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCgcagag                                <  1:885012/150‑1 (MQ=255)
                                                                                                                        cGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCgcagagc                               >  1:1139791/1‑150 (MQ=255)
                                                                                                                             gTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAgaga                          >  1:162757/1‑150 (MQ=255)
                                                                                                                             gTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAgaga                          >  1:93212/1‑150 (MQ=255)
                                                                                                                               tAGCGGCAGAACGCGCGATTGCAGCTGTAGCGGTAATACGACCAATGATCTGAGCGATAATATTCCCCAGTCCCTGTCCATCCAGTTGTCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGAAGAGttt                        <  2:1722189/150‑1 (MQ=255)
                                                                                                                               tAGCGGCAGAACGCGCGATGGCTGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGAtt                        <  2:2280129/150‑1 (MQ=255)
                                                                                                                                 gCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCg                      <  2:3505879/150‑1 (MQ=255)
                                                                                                                                   ggCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGAt                    <  2:5014684/150‑1 (MQ=255)
                                                                                                                                    gCAGAACGCGCGATGGCTGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGAtt                   <  1:3533271/150‑1 (MQ=255)
                                                                                                                                    gCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGAtt                   <  2:1522946/150‑1 (MQ=255)
                                                                                                                                     cAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTg                  <  2:4065533/150‑1 (MQ=255)
                                                                                                                                     cAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTg                  <  2:64472/150‑1 (MQ=255)
                                                                                                                                       gAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTgcg                >  2:4895660/1‑150 (MQ=255)
                                                                                                                                        aaCGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTgcgc               >  2:2605779/1‑150 (MQ=255)
                                                                                                                                        aaCGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTgcgc               >  2:2605907/1‑150 (MQ=255)
                                                                                                                                         aCGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGcc              >  1:2996107/1‑150 (MQ=255)
                                                                                                                                           gcgcgATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGa            >  2:4743313/1‑150 (MQ=255)
                                                                                                                                              cgATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCa         >  2:4234859/1‑150 (MQ=255)
                                                                                                                                                     aGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCAt  >  1:483497/1‑150 (MQ=255)
                                                                                                                                                     aGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCAt  >  2:2760105/1‑150 (MQ=255)
                                                                                                                                                     aGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTAGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCAt  >  1:4525407/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCAT  >  CP009273/1255109‑1255407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑