Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 118,873 G→T 100% D99Y (GAC→TAC)  pdhR → pyruvate dehydrogenase complex repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273118,8730GT99.7% 1067.6 / ‑4.6 326D99Y (GAC→TAC) pdhRpyruvate dehydrogenase complex repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (143/181);  total (145/181)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 326 total aligned reads displayed.

TCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATCA  >  CP009273/118726‑119019
                                                                                                                                                   |                                                                                                                                                  
tcctTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTAc                                                                                                                                                  <  1:2226310/150‑1 (MQ=255)
 cctTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACt                                                                                                                                                 <  2:1239930/150‑1 (MQ=255)
 cctTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACt                                                                                                                                                 <  1:2502568/150‑1 (MQ=255)
 cctTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACt                                                                                                                                                 <  2:592775/150‑1 (MQ=255)
 cctTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACt                                                                                                                                                 >  2:83323/1‑150 (MQ=255)
  ctagCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACtt                                                                                                                                                >  2:2528984/4‑150 (MQ=255)
  ctTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACtt                                                                                                                                                <  1:3958053/150‑1 (MQ=255)
  ctTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTACTCTCCGACCATCCTGAGTCACAGTATTACtt                                                                                                                                                >  2:331612/1‑150 (MQ=255)
   ttGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTg                                                                                                                                               <  2:4699591/150‑1 (MQ=255)
    tGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGc                                                                                                                                              <  1:119643/150‑1 (MQ=255)
        tGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGa                                                                                                                                          <  1:3288755/150‑1 (MQ=255)
            gCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAaca                                                                                                                                      <  1:1958810/150‑1 (MQ=255)
              gATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacg                                                                                                                                    >  2:3840804/1‑150 (MQ=255)
              gATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacg                                                                                                                                    >  2:487626/1‑150 (MQ=255)
              gATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacg                                                                                                                                    >  2:3164716/1‑150 (MQ=255)
                ttCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacgac                                                                                                                                  <  1:2179144/150‑1 (MQ=255)
                     cGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGcc                                                                                                                             >  1:2849805/1‑150 (MQ=255)
                      gTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGccc                                                                                                                            >  2:4555115/1‑150 (MQ=255)
                      gTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGccc                                                                                                                            >  2:1434826/1‑150 (MQ=255)
                      gTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGccc                                                                                                                            >  1:5170795/1‑150 (MQ=255)
                       tctcGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCt                                                                                                                           <  2:1584487/150‑1 (MQ=255)
                        ctcGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTg                                                                                                                          >  1:3841483/1‑150 (MQ=255)
                           gaagcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGaa                                                                                                                       >  1:1008716/1‑150 (MQ=255)
                           gaagcgaagGGCTTGTTGCTTCGTCGCCAGAGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGaa                                                                                                                       >  1:3880285/1‑150 (MQ=255)
                              gcgacgGGCTGGGTGCGTCGTCGCCCGGGTGGCGGCACTTTTGTCCCGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGTGTCACAGTAGTACTTGCTCGAAACACGACACGCCCTGGAAGGt                                                                                                                    <  1:1443338/150‑1 (MQ=255)
                               cgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTa                                                                                                                   <  1:4840833/150‑1 (MQ=255)
                                gaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTAt                                                                                                                  >  2:1374724/1‑150 (MQ=255)
                                gaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTAt                                                                                                                  <  1:1402820/150‑1 (MQ=255)
                                 aagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATc                                                                                                                 >  2:2001883/1‑150 (MQ=255)
                                 aagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATc                                                                                                                 <  2:2744458/150‑1 (MQ=255)
                                 aagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATc                                                                                                                 >  2:867822/1‑150 (MQ=255)
                                  agGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATcg                                                                                                                <  1:4678735/150‑1 (MQ=255)
                                       ttgttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCt                                                                                                           >  2:2470314/1‑150 (MQ=255)
                                       ttgttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCt                                                                                                           >  1:4815613/1‑150 (MQ=255)
                                          ttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttat                                                                                                        <  1:4610375/150‑1 (MQ=255)
                                           tgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttatt                                                                                                       <  2:3388542/150‑1 (MQ=255)
                                           tgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttatt                                                                                                       >  1:5182828/1‑150 (MQ=255)
                                            gCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttatta                                                                                                      <  1:1820349/150‑1 (MQ=255)
                                            gCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttatta                                                                                                      <  1:4567238/150‑1 (MQ=255)
                                             cTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAc                                                                                                     >  1:2434763/1‑150 (MQ=255)
                                                 gtcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAcgccg                                                                                                 <  1:3181119/150‑1 (MQ=255)
                                                     ccAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCt                                                                                             <  1:80942/150‑1 (MQ=255)
                                                      cAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTg                                                                                            >  2:4636464/1‑150 (MQ=255)
                                                           tGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCgtag                                                                                       >  2:561622/1‑150 (MQ=255)
                                                            ggcggcACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACGTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCgtagt                                                                                      <  2:3371662/150‑1 (MQ=255)
                                                              cggcACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTAc                                                                                    <  2:740886/150‑1 (MQ=255)
                                                               ggcACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTAcc                                                                                   >  2:2331205/1‑150 (MQ=255)
                                                               ggcACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTAcc                                                                                   <  1:767715/150‑1 (MQ=255)
                                                                  aCTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGAt                                                                                <  1:254701/150‑1 (MQ=255)
                                                                       tGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGa                                                                           >  1:232733/1‑150 (MQ=255)
                                                                         tCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACa                                                                         <  1:1062243/150‑1 (MQ=255)
                                                                         tCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACa                                                                         <  1:553017/150‑1 (MQ=255)
                                                                            agagCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAgg                                                                      >  1:5028380/1‑150 (MQ=255)
                                                                            agagCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAgg                                                                      <  2:1482895/150‑1 (MQ=255)
                                                                            agagCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAgg                                                                      <  1:3380249/150‑1 (MQ=255)
                                                                             gagCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGa                                                                     >  1:4438625/1‑150 (MQ=255)
                                                                               gcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAAc                                                                   <  1:61749/150‑1 (MQ=255)
                                                                               gcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAAc                                                                   <  1:1276573/150‑1 (MQ=255)
                                                                               gcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAAc                                                                   <  2:1881381/150‑1 (MQ=255)
                                                                               gcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAAc                                                                   <  2:4388088/150‑1 (MQ=255)
                                                                                cagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACg                                                                  >  1:2795708/1‑150 (MQ=255)
                                                                                 agcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGc                                                                 >  1:2625285/1‑150 (MQ=255)
                                                                                   ccTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCAt                                                               >  1:1625176/1‑150 (MQ=255)
                                                                                   ccTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCAt                                                               >  2:2626078/1‑150 (MQ=255)
                                                                                    cTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATc                                                              <  2:3036915/150‑1 (MQ=255)
                                                                                    cTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATc                                                              <  2:635409/150‑1 (MQ=255)
                                                                                    cTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATc                                                              <  1:1733438/150‑1 (MQ=255)
                                                                                     tATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATcc                                                             <  1:3078806/150‑1 (MQ=255)
                                                                                      aTGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCg                                                            >  2:2128570/1‑150 (MQ=255)
                                                                                        ggCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTg                                                          >  2:2853632/1‑150 (MQ=255)
                                                                                          cAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGAATCCGTGat                                                        <  2:270643/150‑2 (MQ=255)
                                                                                                ttCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTccac                                                  <  1:29159/150‑1 (MQ=255)
                                                                                                 tCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTccacc                                                 >  1:1257992/1‑150 (MQ=255)
                                                                                                 tCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTccacc                                                 >  1:700960/1‑150 (MQ=255)
                                                                                                 tCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTccacc                                                 >  1:367770/1‑150 (MQ=255)
                                                                                                   aGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCcaccac                                               >  1:1127163/1‑150 (MQ=255)
                                                                                                   aGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCcaccac                                               >  2:1894287/1‑150 (MQ=255)
                                                                                                      gATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGcc                                            >  2:2235421/1‑150 (MQ=255)
                                                                                                            cTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATagag                                      <  2:4969471/150‑1 (MQ=255)
                                                                                                               gtggAGCTGCTCTCCGACCATCCTGAGTCACTGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTg                                   <  2:1126885/150‑1 (MQ=255)
                                                                                                                  gAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGgcg                                <  2:3924901/150‑1 (MQ=255)
                                                                                                                   aGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGgcgc                               <  1:4976403/150‑1 (MQ=255)
                                                                                                                      tgctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCgcagc                            <  2:2434763/150‑1 (MQ=255)
                                                                                                                       gctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCgcagca                           <  1:3754705/150‑1 (MQ=255)
                                                                                                                          ctcCGACCATCTTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTc                        <  1:4748549/150‑1 (MQ=255)
                                                                                                                          ctcCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTc                        <  1:3528205/150‑1 (MQ=255)
                                                                                                                               aCCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCg                   >  2:418070/1‑150 (MQ=255)
                                                                                                                               aCCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCg                   >  2:4782748/1‑150 (MQ=255)
                                                                                                                               aCCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCg                   >  2:1017943/1‑150 (MQ=255)
                                                                                                                                ccATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGa                  <  1:4504809/150‑1 (MQ=255)
                                                                                                                                  aTCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATc                >  2:1527611/1‑150 (MQ=255)
                                                                                                                                  aTCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCGTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATACGTGAACTACACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATc                >  1:4937720/1‑150 (MQ=255)
                                                                                                                                      tGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGa            >  1:4155473/1‑150 (MQ=255)
                                                                                                                                         gTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGAcgc         >  1:3556723/1‑150 (MQ=255)
                                                                                                                                          tCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGAcgcg        >  1:5242205/1‑150 (MQ=255)
                                                                                                                                            acaGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGa      <  2:3602985/150‑1 (MQ=255)
                                                                                                                                              aGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAAt    >  2:3496226/1‑150 (MQ=255)
                                                                                                                                              aGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAAt    >  2:5195869/1‑150 (MQ=255)
                                                                                                                                                tATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATCa  <  2:4963114/150‑1 (MQ=255)
                                                                                                                                                tATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATCa  <  1:1764320/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATCA  >  CP009273/118726‑119019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑