Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,328,313 C→A 30.1% R68S (CGT→AGT)  cysB → N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,328,3130CA30.1% 507.1 / 198.8 272R68S (CGT→AGT) cysBN‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (93/97);  new base A (47/35);  total (140/132)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 275 total aligned reads displayed.

ATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGA  >  CP009273/1328164‑1328461
                                                                                                                                                     |                                                                                                                                                    
aggTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTc                                                                                                                                                      <  1:3288915/148‑1 (MQ=255)
aTGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTc                                                                                                                                                      <  2:5308383/150‑1 (MQ=255)
 tGTCTCATCAACATCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCg                                                                                                                                                     <  2:4666855/150‑1 (MQ=255)
  gTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGt                                                                                                                                                    >  1:5254220/1‑150 (MQ=255)
      catcaACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTAtcg                                                                                                                                                <  2:4906322/150‑1 (MQ=255)
       atcaACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcgc                                                                                                                                               >  1:4099670/1‑150 (MQ=255)
        tcaACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTAtcgct                                                                                                                                              <  2:3556883/150‑1 (MQ=255)
         caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcgctc                                                                                                                                             >  2:4985946/1‑150 (MQ=255)
         caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTAtcgctc                                                                                                                                             <  2:3796254/150‑1 (MQ=255)
          aaCAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcgctcg                                                                                                                                            >  2:1024744/1‑150 (MQ=255)
          aaCAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcgctcg                                                                                                                                            >  2:5131075/1‑150 (MQ=255)
            cAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCTTATCGCTcgcg                                                                                                                                          <  1:2735584/150‑1 (MQ=255)
                  aaGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTcc                                                                                                                                    >  1:2772092/1‑150 (MQ=255)
                       cTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCg                                                                                                                               >  1:3570752/1‑150 (MQ=255)
                       cTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCg                                                                                                                               >  1:1451482/1‑150 (MQ=255)
                           acacATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAg                                                                                                                           >  1:1582081/1‑150 (MQ=255)
                           acacATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAg                                                                                                                           <  1:3172103/150‑1 (MQ=255)
                            cacaTCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGt                                                                                                                          >  1:3425214/1‑150 (MQ=255)
                            cacaTCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGt                                                                                                                          >  2:1824733/1‑150 (MQ=255)
                                tCACACCCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGAt                                                                                                                      <  2:3337402/150‑1 (MQ=255)
                                    aaCCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCa                                                                                                                  >  1:3648792/1‑150 (MQ=255)
                                    aaCCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCa                                                                                                                  >  2:2390112/1‑150 (MQ=255)
                                    aaCCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCa                                                                                                                  >  1:338521/1‑150 (MQ=255)
                                    aaCCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCa                                                                                                                  >  2:2113272/1‑150 (MQ=255)
                                    aaCCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCa                                                                                                                  <  1:3336216/150‑1 (MQ=255)
                                      cccGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATa                                                                                                                >  2:971286/1‑150 (MQ=255)
                                        cGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATaaa                                                                                                              <  2:4197002/150‑1 (MQ=255)
                                         gggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATaaaa                                                                                                             >  1:2992083/1‑150 (MQ=255)
                                          ggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAAt                                                                                                            <  1:2099084/150‑1 (MQ=255)
                                           gATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATc                                                                                                           >  1:2217669/1‑150 (MQ=255)
                                            aTCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCg                                                                                                          >  2:795320/1‑150 (MQ=255)
                                             tCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCgg                                                                                                         <  2:4777302/150‑1 (MQ=255)
                                              cAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGt                                                                                                        <  2:5242456/150‑1 (MQ=255)
                                               aGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGtt                                                                                                       >  2:1665019/1‑150 (MQ=255)
                                                gTAAACAAGTCAGAATGCTTGAAGACGTGCTAGTAATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGTGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATATAATCGGTTg                                                                                                      <  1:4088878/150‑1 (MQ=255)
                                                gTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTg                                                                                                      <  2:3457950/150‑1 (MQ=255)
                                                gTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTg                                                                                                      >  1:918119/1‑150 (MQ=255)
                                                gTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTg                                                                                                      >  1:1380173/1‑150 (MQ=255)
                                                    aCAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCgg                                                                                                  >  1:424870/1‑150 (MQ=255)
                                                       aGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGgaga                                                                                               <  1:3878019/150‑1 (MQ=255)
                                                        gTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGagag                                                                                              >  2:2957657/1‑150 (MQ=255)
                                                             aaTGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCacac                                                                                         >  1:2348369/1‑150 (MQ=255)
                                                                gCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTg                                                                                      <  1:1495556/150‑1 (MQ=255)
                                                                 cTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTgg                                                                                     >  2:1331428/1‑150 (MQ=255)
                                                                   ggAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGcc                                                                                   >  2:2461451/1‑150 (MQ=255)
                                                                      aGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGa                                                                                <  1:971286/150‑1 (MQ=255)
                                                                          gAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATaaa                                                                            <  1:1159797/150‑1 (MQ=255)
                                                                          gAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATaaa                                                                            <  2:262013/150‑1 (MQ=255)
                                                                          gAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATaaa                                                                            <  2:4397455/150‑1 (MQ=255)
                                                                           aGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAg                                                                           >  2:547010/1‑150 (MQ=255)
                                                                            gCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAgg                                                                          <  2:4456741/150‑1 (MQ=255)
                                                                                ggCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCa                                                                      <  1:4310192/150‑1 (MQ=255)
                                                                                 gCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCAc                                                                     <  1:3677096/150‑1 (MQ=255)
                                                                                 gCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCAc                                                                     >  2:1125971/1‑150 (MQ=255)
                                                                                  cATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACt                                                                    <  1:3134411/150‑1 (MQ=255)
                                                                                   aTTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTg                                                                   >  2:1576953/1‑150 (MQ=255)
                                                                                   aTTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTg                                                                   <  2:3401097/150‑1 (MQ=255)
                                                                                   aTTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTg                                                                   >  2:283625/1‑150 (MQ=255)
                                                                                     tCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGta                                                                 <  2:1964696/150‑1 (MQ=255)
                                                                                     tCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGta                                                                 >  2:1859550/1‑150 (MQ=255)
                                                                                        aaTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTatat                                                              <  2:755128/150‑1 (MQ=255)
                                                                                        aaTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTatat                                                              <  2:5274915/150‑1 (MQ=255)
                                                                                         aTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATc                                                             <  1:3739552/150‑1 (MQ=255)
                                                                                         aTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATc                                                             <  1:480649/150‑1 (MQ=255)
                                                                                          ttttttCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCg                                                            <  2:3912790/150‑1 (MQ=255)
                                                                                                cccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGccacca                                                      >  1:720684/1‑150 (MQ=255)
                                                                                                 ccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCcaccac                                                     >  2:4201149/1‑150 (MQ=255)
                                                                                                     aGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCAt                                                 >  1:1892715/1‑150 (MQ=255)
                                                                                                     aGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCAt                                                 >  1:4559694/1‑150 (MQ=255)
                                                                                                        ggCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCAAAcc                                              >  1:5111767/1‑150 (MQ=255)
                                                                                                        ggCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATAcc                                              >  1:5147306/1‑150 (MQ=255)
                                                                                                          cAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCa                                            >  2:2160260/1‑150 (MQ=255)
                                                                                                            aGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAgg                                          <  1:2538507/150‑1 (MQ=255)
                                                                                                              cACCTGACGCAGGTAACGGCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGTAGTCATGTCTTTAGTCGATGCCATTAATTCGGTTGCCGTATATCACACCTGGACGTATAAATGTTCACTTTATATCGCCACCAAGAATAcggagggt                                        <  2:2999037/150‑8 (MQ=255)
                                                                                                              cACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCa                                        >  1:2656816/1‑150 (MQ=255)
                                                                                                              cACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCa                                        >  2:2247553/1‑150 (MQ=255)
                                                                                                                  tGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCt                                    <  1:4924885/150‑1 (MQ=255)
                                                                                                                  tGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCt                                    >  1:4720166/1‑150 (MQ=255)
                                                                                                                   gACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTa                                   <  2:614537/150‑1 (MQ=255)
                                                                                                                   gACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTa                                   <  1:12801/150‑1 (MQ=255)
                                                                                                                       cAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCa                               <  1:1024744/150‑1 (MQ=255)
                                                                                                                       cAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCa                               <  1:5131075/150‑1 (MQ=255)
                                                                                                                           tAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTAc                           >  1:4691357/1‑150 (MQ=255)
                                                                                                                             aCGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCa                         <  2:1417318/150‑1 (MQ=255)
                                                                                                                                ccAGCAGGGCTAGTAATAATTCGTATCGCTCGCGAAGTCCTGTCTAAAGTCGATGCCATAAAATCGGTTGCCTGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAAc                      <  2:5111767/150‑1 (MQ=255)
                                                                                                                                ccAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAAc                      >  1:3034754/1‑150 (MQ=255)
                                                                                                                                 cagcagGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACg                     >  1:4981208/1‑150 (MQ=255)
                                                                                                                                  agcagGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGt                    >  1:1738985/1‑150 (MQ=255)
                                                                                                                                      gggCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGtcatc                >  2:4050348/1‑150 (MQ=255)
                                                                                                                                        gCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCaa              <  2:1811483/150‑1 (MQ=255)
                                                                                                                                           aGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAgg           >  2:2591971/1‑150 (MQ=255)
                                                                                                                                           aGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAgg           <  1:2390112/150‑1 (MQ=255)
                                                                                                                                            gAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGc          >  2:3712996/1‑150 (MQ=255)
                                                                                                                                             aaaTAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCt         <  1:4439761/150‑1 (MQ=255)
                                                                                                                                                taatTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTa      >  2:4694117/1‑150 (MQ=255)
                                                                                                                                                taatTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTa      >  1:1958772/1‑150 (MQ=255)
                                                                                                                                                 aatTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTAt     >  2:5032360/1‑150 (MQ=255)
                                                                                                                                                  atTCGTATCGCTCGCGAAGTCCTGTCGACAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTAtt    <  2:3215356/150‑1 (MQ=255)
                                                                                                                                                   ttCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTg   <  2:3648792/150‑1 (MQ=255)
                                                                                                                                                    tCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGa  <  1:3846036/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGA  >  CP009273/1328164‑1328461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑